FastQCFastQC Report
Wed 25 May 2016
SRR1780214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135665
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1383910.200862418457229No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC38342.8260789444587773No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG36002.653595253012936No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG33832.4936424280396565TruSeq Adapter, Index 12 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC9140.6737183503482844No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4200.3095861128515092TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2810.20712785169350972No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2130.1570043858032654No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2110.15553016621825821No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1740.12825710389562525No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1560.11498912763056057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT150.002220665370.011
ATAACCC150.002220665370.028
TTGCATA150.002220665370.01
TCTTTAT1550.063.2258071
GAAAGCT308.340483E-658.33333234
ATTAACG200.00693889452.49999618
GCGTACA200.00693889452.49999670
TAGTAAC200.00693889452.49999634
TTTATAC2100.051.6666643
CTTTATA1950.050.256412
TGAAAGC352.0776595E-550.033
TTGAAAG352.0776595E-550.032
AAAGCTC459.159048E-538.88888535
AAATACA400.002411944834.99999670
CTATACA552.979655E-431.818181
AAAAAGC552.979655E-431.8181868
GCTCGTA552.979655E-431.8181838
ATCACAC450.00429520731.11110917
AAAAATG450.00429520731.11110968
GTCCAAG450.00429520731.1111091
Produced by FastQC (version 0.11.5)