##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780214_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135665 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73450779493606 34.0 34.0 34.0 34.0 34.0 2 33.725596137544684 34.0 34.0 34.0 34.0 34.0 3 33.739844469833784 34.0 34.0 34.0 34.0 34.0 4 33.762569564736665 34.0 34.0 34.0 34.0 34.0 5 33.761515497733384 34.0 34.0 34.0 34.0 34.0 6 37.50696937308812 38.0 38.0 38.0 37.0 38.0 7 37.52407769137213 38.0 38.0 38.0 37.0 38.0 8 37.41733682231968 38.0 38.0 38.0 37.0 38.0 9 37.46050934286662 38.0 38.0 38.0 37.0 38.0 10-11 37.38409685622673 38.0 38.0 38.0 37.0 38.0 12-13 37.382600523347946 38.0 38.0 38.0 37.0 38.0 14-15 37.31522868831313 38.0 38.0 38.0 36.0 38.0 16-17 37.326701802233444 38.0 38.0 38.0 36.0 38.0 18-19 37.25415545645524 38.0 38.0 38.0 36.0 38.0 20-21 37.3026351675082 38.0 38.0 38.0 36.0 38.0 22-23 37.29445693436037 38.0 38.0 38.0 36.0 38.0 24-25 37.304441086499835 38.0 38.0 38.0 36.0 38.0 26-27 37.21159842258504 38.0 38.0 38.0 36.0 38.0 28-29 37.17225887295913 38.0 38.0 38.0 36.0 38.0 30-31 37.149773338738804 38.0 38.0 38.0 36.0 38.0 32-33 37.08037076622563 38.0 38.0 38.0 36.0 38.0 34-35 37.07092838978366 38.0 38.0 38.0 36.0 38.0 36-37 37.04551284413813 38.0 38.0 38.0 36.0 38.0 38-39 36.32073489846313 38.0 37.0 38.0 34.0 38.0 40-41 36.709261784542804 38.0 37.0 38.0 34.5 38.0 42-43 37.02731728891018 38.0 38.0 38.0 36.0 38.0 44-45 37.119150112409244 38.0 38.0 38.0 36.0 38.0 46-47 37.059153060848416 38.0 38.0 38.0 36.0 38.0 48-49 37.08587329082667 38.0 38.0 38.0 36.0 38.0 50-51 36.920373714664805 38.0 38.0 38.0 35.0 38.0 52-53 36.90218184498581 38.0 38.0 38.0 35.0 38.0 54-55 36.8772269929606 38.0 37.5 38.0 35.5 38.0 56-57 36.92205801054067 38.0 38.0 38.0 35.0 38.0 58-59 36.77503040577894 38.0 37.5 38.0 34.5 38.0 60-61 36.773150775808055 38.0 37.0 38.0 34.5 38.0 62-63 36.7469907492721 38.0 37.0 38.0 34.5 38.0 64-65 36.60484649688571 38.0 37.0 38.0 34.5 38.0 66-67 36.17844322411823 38.0 37.0 38.0 33.5 38.0 68-69 36.02823867615081 38.0 37.0 38.0 33.0 38.0 70-71 35.01770169166697 38.0 36.5 38.0 27.0 38.0 72-73 33.265975011978036 38.0 34.0 38.0 16.0 38.0 74-75 32.733431614639 38.0 33.0 38.0 11.0 38.0 76 32.13733829653927 37.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.031022724766096132 1101 2 0.013539620659159368 1101 3 -0.03191989413235774 1101 4 0.06540025155878482 1101 5 -0.14828063810024616 1101 6 -0.050638650174107624 1101 7 -0.15885304040963177 1101 8 -0.03652746938997353 1101 9 -0.10656403507466905 1101 10-11 -0.08080166738694317 1101 12-13 0.03865154577589891 1101 14-15 0.2410195518233209 1101 16-17 0.14880976397073908 1101 18-19 0.1388431767578524 1101 20-21 0.10136497319526683 1101 22-23 -0.0035337772529331346 1101 24-25 0.02113898662404523 1101 26-27 -0.06861736958760645 1101 28-29 -0.2039530518863657 1101 30-31 -0.11923571117127096 1101 32-33 -0.21483444653644312 1101 34-35 -0.01711270436941703 1101 36-37 0.03730939628493246 1101 38-39 0.26198276321545677 1101 40-41 -0.21686266298822687 1101 42-43 0.1089254520580738 1101 44-45 0.16218749789764075 1101 46-47 0.16390119555176597 1101 48-49 0.1435219087435442 1101 50-51 0.11814617388840531 1101 52-53 0.05481104451261842 1101 54-55 -0.024170855191457008 1101 56-57 -0.07384860125992532 1101 58-59 -0.25460806008077697 1101 60-61 -0.1009260492456292 1101 62-63 -0.05172126645927477 1101 64-65 -0.03663122428980614 1101 66-67 -0.4105130008901199 1101 68-69 -0.8588807439075765 1101 70-71 -0.3093658119480338 1101 72-73 -0.24891156386789248 1101 74-75 -0.4885053139342048 1101 76 -1.4576434740200916 1102 1 0.011159064233908111 1102 2 0.0833203506070177 1102 3 -0.10900962581818874 1102 4 0.019324177627552785 1102 5 0.05495881305007089 1102 6 0.003397172663945014 1102 7 0.0702337708498817 1102 8 -0.12543381734924708 1102 9 0.08643805207783828 1102 10-11 0.06266427552189668 1102 12-13 0.04910318018296067 1102 14-15 0.09483951706390314 1102 16-17 -0.0248722746138057 1102 18-19 -0.03844177907863866 1102 20-21 0.09694068604117234 1102 22-23 -0.12541792838499788 1102 24-25 -0.16687139358688086 1102 26-27 -0.07489469898470702 1102 28-29 0.1843446093917933 1102 30-31 0.10727588720662595 1102 32-33 0.2107899136283038 1102 34-35 0.09116924374540503 1102 36-37 0.08399256970904645 1102 38-39 0.10177595259081329 1102 40-41 0.21612625050208578 1102 42-43 0.02013020727397219 1102 44-45 0.08091552526076384 1102 46-47 0.21333020459080387 1102 48-49 0.20345810377722984 1102 50-51 -0.05300826746102416 1102 52-53 0.2214380314738662 1102 54-55 0.2454329032702418 1102 56-57 0.18866439384359524 1102 58-59 -0.013724472400532761 1102 60-61 -0.05462776896406041 1102 62-63 -0.01447789495406937 1102 64-65 0.2505585796130916 1102 66-67 0.16531094819288228 1102 68-69 0.09720572610646627 1102 70-71 0.20954950362465752 1102 72-73 -0.11632182821408321 1102 74-75 -0.4833985358469235 1102 76 0.18741362507600456 1103 1 0.005486028806863885 1103 2 0.0614991728440657 1103 3 0.08684741055044043 1103 4 0.030835025444261532 1103 5 0.12068128156590063 1103 6 -0.13176701580951544 1103 7 0.07106550218496466 1103 8 -0.25190405941444993 1103 9 -0.0512291769229094 1103 10-11 0.04385223895710055 1103 12-13 0.011541879229604035 1103 14-15 -0.003316550259256701 1103 16-17 -0.010136506243135557 1103 18-19 -0.08389668119327354 1103 20-21 0.07729691814130035 1103 22-23 -0.06868365085350803 1103 24-25 -0.14998175491922439 1103 26-27 0.03665930470315715 1103 28-29 -0.0033912766767016933 1103 30-31 -0.31245026917455476 1103 32-33 -0.25525818555173885 1103 34-35 -0.308136339055018 1103 36-37 0.03204074735430851 1103 38-39 -0.04930490581869407 1103 40-41 -0.25778798701214356 1103 42-43 -0.1909020026989552 1103 44-45 -0.13524438685819007 1103 46-47 -0.239979066074973 1103 48-49 -0.2536371137675957 1103 50-51 -0.358536142230534 1103 52-53 -0.09966911278444002 1103 54-55 -0.35897684211012404 1103 56-57 -0.408718791059691 1103 58-59 0.2481767398871213 1103 60-61 -0.0634315666807268 1103 62-63 -0.2737348293179025 1103 64-65 -0.2850803234431325 1103 66-67 -0.654568215367874 1103 68-69 -0.17897223301314824 1103 70-71 0.19245193212322675 1103 72-73 0.3822147954274868 1103 74-75 0.7274571273803545 1103 76 0.669927650734131 1104 1 0.0853692497008538 1104 2 0.06826920480050802 1104 3 0.10741789226425169 1104 4 0.18331066358683756 1104 5 0.02321018044939649 1104 6 0.1014597960419863 1104 7 -0.19826025628743338 1104 8 -0.18831468000071538 1104 9 -0.05343851136090905 1104 10-11 0.11143420131951132 1104 12-13 -0.05920811090728506 1104 14-15 0.055140071826194514 1104 16-17 -0.0777697567264255 1104 18-19 -0.07084582704879949 1104 20-21 0.08507298631504767 1104 22-23 0.03934491268457663 1104 24-25 0.08172219426608507 1104 26-27 0.13323089108418884 1104 28-29 0.07979805539415707 1104 30-31 0.177299997129154 1104 32-33 0.1282309847642793 1104 34-35 0.1496377564986986 1104 36-37 0.11278403058309294 1104 38-39 0.017555077611298486 1104 40-41 0.061729115603554874 1104 42-43 -0.1310132584688688 1104 44-45 0.19823097511759613 1104 46-47 -0.01905745799511749 1104 48-49 0.09173709225284199 1104 50-51 -0.24973431163914483 1104 52-53 -0.14759983436884738 1104 54-55 -0.21754393091747204 1104 56-57 -0.19728943058310477 1104 58-59 0.2649519006556531 1104 60-61 0.2365131999583312 1104 62-63 0.12132573011713532 1104 64-65 0.01855324265936531 1104 66-67 -0.27944689923578636 1104 68-69 -0.25649620034656095 1104 70-71 -0.39418948500700424 1104 72-73 0.7057797143528006 1104 74-75 0.45267115665403423 1104 76 0.6037896462365566 1105 1 -0.19832200457863536 1105 2 0.07947368659322507 1105 3 0.007355645143178435 1105 4 -0.04326885711032702 1105 5 0.08452359470398818 1105 6 0.02567392613846664 1105 7 -0.022100903657495508 1105 8 -0.15252258538509267 1105 9 0.05440462589399431 1105 10-11 0.06887273228745983 1105 12-13 0.0308167879411414 1105 14-15 0.0026190634228342446 1105 16-17 0.008598123759092857 1105 18-19 0.096209884124562 1105 20-21 0.13860551043580926 1105 22-23 0.03055250683335231 1105 24-25 -0.2643717988867351 1105 26-27 0.05378800281777529 1105 28-29 0.027121429188113666 1105 30-31 0.13178178984622946 1105 32-33 0.02163278993079132 1105 34-35 -0.09413753139424585 1105 36-37 0.07037817935371038 1105 38-39 -0.053951302718616034 1105 40-41 0.04188784576228244 1105 42-43 -0.019279676147050395 1105 44-45 -0.2236488879387437 1105 46-47 -0.30679477514419773 1105 48-49 -0.22074345552181995 1105 50-51 -0.44402313828091167 1105 52-53 0.10924734894892651 1105 54-55 -0.12378420480480656 1105 56-57 -0.1699006973120163 1105 58-59 0.08778103230831391 1105 60-61 0.011100812781229763 1105 62-63 -0.043784758522761535 1105 64-65 -0.3259067635653423 1105 66-67 -0.3409159312930541 1105 68-69 -0.04080992583676135 1105 70-71 -0.07462832721055435 1105 72-73 0.28008721513089085 1105 74-75 0.5763095008806189 1105 76 0.8642938479172315 1106 1 0.09213421983984915 1106 2 0.0057989336731907315 1106 3 -0.16450219652598008 1106 4 -0.021805345080778693 1106 5 0.009151726879295552 1106 6 -0.015605898160224285 1106 7 -0.06962012098803427 1106 8 0.17292415515598947 1106 9 0.03957982805032856 1106 10-11 -0.0181044065764695 1106 12-13 0.10571446189541689 1106 14-15 0.11110962946057157 1106 16-17 0.21180267602025538 1106 18-19 0.1486457431641952 1106 20-21 0.2193682138400206 1106 22-23 0.11872760915940006 1106 24-25 0.20513004797360423 1106 26-27 -0.047414952158767676 1106 28-29 0.08175355457495215 1106 30-31 0.23667846549763283 1106 32-33 0.09715440318825586 1106 34-35 -0.14397780293262485 1106 36-37 -0.013504220566424863 1106 38-39 0.030080842634291116 1106 40-41 0.15472102935019194 1106 42-43 0.2162199744469433 1106 44-45 0.08417780668040109 1106 46-47 0.029433636555907583 1106 48-49 0.1301600085891934 1106 50-51 0.231054230192683 1106 52-53 0.08256770434475413 1106 54-55 0.2931320375831348 1106 56-57 0.29975089498108076 1106 58-59 0.1940254168545863 1106 60-61 0.1754963403843064 1106 62-63 0.2971356767372981 1106 64-65 0.10758130671517563 1106 66-67 0.05121744197239764 1106 68-69 0.08542547398354827 1106 70-71 -0.09986043263133837 1106 72-73 -0.11885957532533098 1106 74-75 -0.18841546667454168 1106 76 0.16887605815981743 1107 1 -0.09974260325950723 1107 2 0.006936178439275409 1107 3 0.07785647865771494 1107 4 0.1326679407442768 1107 5 0.09332260898245437 1107 6 0.10469835173823583 1107 7 0.24710254636787 1107 8 0.2070193440198551 1107 9 0.26673339301728305 1107 10-11 0.17161608229108083 1107 12-13 0.310750831573813 1107 14-15 0.20447816322714374 1107 16-17 0.16047896742076517 1107 18-19 0.02924242743107186 1107 20-21 0.18107851181292034 1107 22-23 0.17194918727112452 1107 24-25 0.2571183801376904 1107 26-27 0.3568792545391588 1107 28-29 0.3938497034506341 1107 30-31 0.35797226603671106 1107 32-33 0.2749559761306841 1107 34-35 0.27166300495424167 1107 36-37 0.4501962557471373 1107 38-39 0.2338322734211289 1107 40-41 0.4993025011221448 1107 42-43 0.26910185607010106 1107 44-45 0.29421731485896885 1107 46-47 0.2801444145347176 1107 48-49 0.31072715403097817 1107 50-51 0.429713906004082 1107 52-53 0.33853270083701403 1107 54-55 0.32035944961282325 1107 56-57 0.03758643757237223 1107 58-59 0.01240595076884432 1107 60-61 0.09246270304723225 1107 62-63 0.3390955777822313 1107 64-65 0.5244701299622321 1107 66-67 0.547052467634245 1107 68-69 0.4501228856439923 1107 70-71 0.2701096593082539 1107 72-73 0.5632103578430758 1107 74-75 0.6507640924584948 1107 76 0.6670335739446287 1108 1 0.07136364745995394 1108 2 0.09347928883411782 1108 3 0.12982685584968578 1108 4 0.02924903893698172 1108 5 0.07082922806844749 1108 6 0.039835146182042536 1108 7 0.12386859525317817 1108 8 0.10860408750628636 1108 9 0.08101527015169552 1108 10-11 0.034403388994583395 1108 12-13 -0.026995225753232432 1108 14-15 0.196596654459249 1108 16-17 0.03007338052847075 1108 18-19 0.21626901888957661 1108 20-21 0.18031108155314257 1108 22-23 0.09816844209634468 1108 24-25 0.10833283852378628 1108 26-27 0.11362304794693756 1108 28-29 0.28568040833533104 1108 30-31 -0.0860053249997037 1108 32-33 0.13243266543655352 1108 34-35 0.10902151000009752 1108 36-37 0.2500389325438803 1108 38-39 -0.06647853583407937 1108 40-41 0.11354984389486589 1108 42-43 0.24573744181124368 1108 44-45 0.05537383226045023 1108 46-47 0.13500416665473836 1108 48-49 0.10434213426965044 1108 50-51 0.04158221497150549 1108 52-53 0.17452901717177127 1108 54-55 0.11718996263995507 1108 56-57 0.16685622768019925 1108 58-59 0.019853861439962373 1108 60-61 0.20850199547653148 1108 62-63 -5.230093786678935E-4 1108 64-65 0.24684455918597337 1108 66-67 0.0692925965625335 1108 68-69 0.2771172450315902 1108 70-71 0.4769589743767497 1108 72-73 -0.1555648234623277 1108 74-75 -0.15937366127313624 1108 76 0.3320809627631718 1109 1 -0.09079670739816947 1109 2 0.08427348449647099 1109 3 0.15523012060386066 1109 4 -0.02812563507055188 1109 5 0.11598893651415665 1109 6 -0.13208083506457058 1109 7 0.04018419311036325 1109 8 -0.13594784467241539 1109 9 0.026553167008053435 1109 10-11 -0.25218392497609443 1109 12-13 -0.0715753920372535 1109 14-15 -0.13445937537974118 1109 16-17 -0.050178348810476336 1109 18-19 0.0745932504389657 1109 20-21 0.07076275293787404 1109 22-23 -0.06542590811058346 1109 24-25 0.0036028828810472646 1109 26-27 -0.22531252628275666 1109 28-29 -0.05462600600759515 1109 30-31 -0.06510465704020874 1109 32-33 -0.03387364488598621 1109 34-35 -0.22145497767720457 1109 36-37 0.005724761252018595 1109 38-39 0.03971142025445573 1109 40-41 -0.031677477803036425 1109 42-43 -0.08033675444551136 1109 44-45 0.1500546754199945 1109 46-47 0.01879853320772895 1109 48-49 0.11714407202087074 1109 50-51 -0.0461626973170155 1109 52-53 0.1583575618522417 1109 54-55 0.05987472885412615 1109 56-57 0.050918075230889315 1109 58-59 0.17225115988188833 1109 60-61 -0.04252149084107515 1109 62-63 0.008500759279996828 1109 64-65 0.02624332134335816 1109 66-67 -0.29753530170552267 1109 68-69 -0.28888369115190926 1109 70-71 -0.11455296196277942 1109 72-73 -0.146593064997667 1109 74-75 0.07471307687040252 1109 76 0.3231797895617916 1110 1 0.05972292559744119 1110 2 0.013119275976521294 1110 3 0.072886046739157 1110 4 0.07014080547966017 1110 5 0.041761863417669076 1110 6 -0.03630022599961791 1110 7 -0.20850041792586183 1110 8 -0.07879546247764324 1110 9 -0.09040411727540487 1110 10-11 0.032095908625379366 1110 12-13 0.05753202777113131 1110 14-15 -0.040120983415569356 1110 16-17 0.19738292729800122 1110 18-19 -0.03983834208759873 1110 20-21 0.0814453057346185 1110 22-23 -0.02055773689984619 1110 24-25 -0.003941579440116527 1110 26-27 -0.009465531747238742 1110 28-29 0.06038537228812402 1110 30-31 0.16125095456129657 1110 32-33 0.264498606567102 1110 34-35 0.0042664932392924015 1110 36-37 0.11548183101474052 1110 38-39 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60-61 0.14177777154038296 2115 62-63 0.04320516682332709 2115 64-65 0.08474410256497578 2115 66-67 0.18677849503151123 2115 68-69 0.21176672126043172 2115 70-71 -0.2189314365821602 2115 72-73 -0.1587074503760988 2115 74-75 -0.4547153595962996 2115 76 -0.54392533016496 2116 1 0.08230477604814723 2116 2 0.047727654847193435 2116 3 0.08773822552576149 2116 4 -0.04286185670332543 2116 5 0.0653945755749703 2116 6 0.006341406805951522 2116 7 -0.04489292644952059 2116 8 0.06888563602313269 2116 9 0.12420519569456445 2116 10-11 0.21834363175835847 2116 12-13 0.15973416685852015 2116 14-15 -0.07806876726499468 2116 16-17 -0.3654352502742739 2116 18-19 0.06476910268378333 2116 20-21 -0.08382021198990941 2116 22-23 -0.041995315714473236 2116 24-25 0.04678001226508144 2116 26-27 0.2205564195861882 2116 28-29 0.0524572045238898 2116 30-31 0.19842810649255682 2116 32-33 0.007387775685771203 2116 34-35 0.11109242383571427 2116 36-37 -0.008274649299117698 2116 38-39 0.26198276321545677 2116 40-41 0.006784060658496571 2116 42-43 -0.2500489069162768 2116 44-45 -0.16259882688868288 2116 46-47 0.054214585865160814 2116 48-49 -0.18126441604278654 2116 50-51 -0.011483455741228 2116 52-53 0.06620705590863452 2116 54-55 0.08978925876866128 2116 56-57 0.11133658392526513 2116 58-59 0.044537239064517564 2116 60-61 0.15975771143812523 2116 62-63 0.19471747997947375 2116 64-65 0.1627989751403902 2116 66-67 0.2803701899930644 2116 68-69 0.14824176321492644 2116 70-71 0.27610427352205136 2116 72-73 0.24111692616060054 2116 74-75 0.14539781996893453 2116 76 0.11215709578048205 2117 1 0.04712683409054108 2117 2 0.04396117650444609 2117 3 -0.046406349296781 2117 4 0.018922874572929516 2117 5 -0.015198403323090304 2117 6 0.18565957764954533 2117 7 0.06421422503051843 2117 8 -0.28538062502278905 2117 9 0.10242722476404964 2117 10-11 0.08745850934781174 2117 12-13 -0.04812232065898314 2117 14-15 0.14305489877392574 2117 16-17 -0.1306943163807972 2117 18-19 0.09313841071410423 2117 20-21 -0.08410994109320313 2117 22-23 0.06046013843589293 2117 24-25 -0.03658953720616864 2117 26-27 0.055760920044924944 2117 28-29 -0.7134175359271211 2117 30-31 0.030288650217499935 2117 32-33 -0.3052661429003507 2117 34-35 -0.29178796633281223 2117 36-37 -0.13155438274834097 2117 38-39 0.049043944344433044 2117 40-41 -0.3737026842350204 2117 42-43 -0.3453939259901162 2117 44-45 -0.14520300864541014 2117 46-47 -0.003212136815804456 2117 48-49 -0.162250943639485 2117 50-51 -0.13656192404003775 2117 52-53 -0.32196958226801087 2117 54-55 -0.5086824202114997 2117 56-57 -0.36773048836215594 2117 58-59 -0.37468870134785703 2117 60-61 -0.4230082357345921 2117 62-63 -0.1141820323098699 2117 64-65 -0.37603679674622015 2117 66-67 -0.2406110259034051 2117 68-69 -0.4675653737786476 2117 70-71 -0.2137189917249458 2117 72-73 -0.18065621595152948 2117 74-75 -0.022403619018945164 2117 76 -1.1385310108398308 2118 1 0.05035524409861125 2118 2 -0.05005012293058542 2118 3 -0.12417874639120896 2118 4 -0.19512070896217892 2118 5 -0.1379102677298718 2118 6 -0.19792395745941604 2118 7 0.0049890434324524335 2118 8 -0.06119983406233587 2118 9 0.03440462589399118 2118 10-11 0.011518234932964333 2118 12-13 0.10124006836441168 2118 14-15 -0.0730952222914496 2118 16-17 0.05161399677496803 2118 18-19 -0.11431921640453879 2118 20-21 0.05103937286967408 2118 22-23 -0.12701363073278316 2118 24-25 0.006316031801098632 2118 26-27 -0.27674109771133004 2118 28-29 0.03161878368546667 2118 30-31 -0.22488487682043257 2118 32-33 -0.11386985557185625 2118 34-35 -0.1354602827169913 2118 36-37 -0.050997482021948315 2118 38-39 -0.12765500642231586 2118 40-41 -0.023985170110726983 2118 42-43 0.06209598522860915 2118 44-45 -0.01274941703927368 2118 46-47 -0.07690059525002368 2118 48-49 -0.09434133911970122 2118 50-51 -0.08169509595286684 2118 52-53 -0.3795886298870528 2118 54-55 0.14822637720577347 2118 56-57 0.07713633972501555 2118 58-59 -0.12317134864407109 2118 60-61 0.08670927838969789 2118 62-63 0.07743217269415936 2118 64-65 -0.2179173455759269 2118 66-67 0.2717295713524521 2118 68-69 0.012999597972765287 2118 70-71 0.03124468866246133 2118 72-73 0.3056956807393547 2118 74-75 0.2338756384467544 2118 76 -0.26999186636848194 2119 1 0.07488192223974721 2119 2 -0.043303134978778246 2119 3 -0.01843003726900605 2119 4 -0.05648043357491872 2119 5 -0.08815123158529303 2119 6 0.00825504426175172 2119 7 -0.08189563815102474 2119 8 0.023498961118384898 2119 9 -0.03595681988913668 2119 10-11 -0.1653578407985421 2119 12-13 -0.04805425177327294 2119 14-15 -0.22844806222260416 2119 16-17 -0.19562352473965205 2119 18-19 -0.07359632483827738 2119 20-21 0.024060026914419552 2119 22-23 0.08941494069578226 2119 24-25 -0.0694412831730844 2119 26-27 0.084928437572664 2119 28-29 -0.0682521285348443 2119 30-31 -0.1902205050476269 2119 32-33 -0.5840677901673814 2119 34-35 -0.10750847548807485 2119 36-37 -0.023656690283566206 2119 38-39 -0.48678581537240007 2119 40-41 -0.028202037580605577 2119 42-43 0.06700992670882044 2119 44-45 -0.06922962357971585 2119 46-47 0.11470869907011405 2119 48-49 -0.004943748758257982 2119 50-51 0.10626531712803455 2119 52-53 0.04650431331432259 2119 54-55 0.02836407083744774 2119 56-57 -0.19568637972420788 2119 58-59 -0.0195052557008637 2119 60-61 0.15893819405234666 2119 62-63 0.04126177682497456 2119 64-65 -0.23407913559314153 2119 66-67 -0.14119641832053276 2119 68-69 -0.2805546188947048 2119 70-71 -0.15105307381602273 2119 72-73 -0.44126645351193616 2119 74-75 -0.4754531050988575 2119 76 -1.903778441239396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 2.0 23 10.0 24 37.0 25 72.0 26 123.0 27 242.0 28 444.0 29 787.0 30 1207.0 31 1902.0 32 2983.0 33 5053.0 34 8786.0 35 14959.0 36 21659.0 37 77395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.23211587365938 14.170935760881584 12.537500460693622 39.05944790476541 2 18.45354365532746 16.98227250949029 37.98695315667268 26.57723067850956 3 18.172704824383594 15.353259868057348 34.529171120038335 31.944864187520732 4 15.084214793793535 25.549699627759555 29.24040835882505 30.12567721962186 5 15.773412449784397 27.55095271440681 40.4312092286146 16.24442560719419 6 29.30232558139535 37.555743928058085 15.146132016363836 17.995798474182727 7 26.726863966387793 22.515755721814763 20.64791950761066 30.10946080418678 8 22.79585744296613 38.05550436737552 14.0920650130837 25.056573176574652 9 21.05185567390263 14.927210407990271 14.400914016142705 49.6200199019644 10-11 26.61777171709726 26.54774628680942 21.89326650204548 24.94121549404784 12-13 25.06431282939594 16.397375889138686 25.44281870784653 33.09549257361884 14-15 22.21317215199204 15.120701728522462 18.526886079681567 44.13924003980393 16-17 30.288947038661405 23.45041093870932 15.873659381564883 30.386982641064385 18-19 33.490951977297016 21.17532156414698 16.434968488556372 28.898757969999632 20-21 25.671322743522644 18.310544355581765 16.39553311465743 39.62259978623816 22-23 23.388862271035272 23.82154571923488 17.224044521431466 35.565547488298385 24-25 30.4617992850035 25.441344488261525 15.791103084804481 28.305753141930488 26-27 31.406405484096855 26.114325728817306 19.178122581358494 23.301146205727346 28-29 26.092949544834703 21.473482475214684 22.680131205543066 29.75343677440755 30-31 22.833818597280064 25.629307485349944 20.510079976412488 31.026793940957504 32-33 22.05358788191501 19.250359341023845 31.036744923156306 27.659307853904842 34-35 29.356871706040614 22.142778166807947 24.537647882652124 23.96270224449932 36-37 25.776360888954407 32.21022371282202 18.60944237644197 23.403973021781592 38-39 21.265248958832416 23.47436700696569 26.79430951240187 28.46607452180002 40-41 22.396712490325434 16.257693583459258 28.283271293259133 33.06232263295618 42-43 28.95146132016364 15.825010135259646 27.304020933918103 27.919507610658606 44-45 21.403825599823094 23.50127151439207 31.031216599712526 24.06368628607231 46-47 26.259904912836767 18.821729996683008 27.004754358161648 27.913610732318578 48-49 26.293443408395678 16.977481295839013 21.955552279512034 34.77352301625327 50-51 23.004459514244648 17.6825268123687 27.44775734345631 31.865256329930343 52-53 17.767294438506614 17.722330741163898 37.83510853941694 26.675266280912542 54-55 19.055025246010395 18.784874507057825 31.419304905465665 30.74079534146611 56-57 24.542807651199645 20.31179744222902 23.783584564920947 31.361810341650386 58-59 20.30221501492647 20.736372682711092 31.71709726163712 27.244315040725315 60-61 25.05030774333837 26.31113404341577 24.257177606604504 24.381380606641358 62-63 19.150112409243356 37.2712195481517 18.633766999594588 24.944901043010358 64-65 17.485718497770243 39.83230752220543 17.976633619577637 24.70534036044669 66-67 17.974053735303876 39.412892050270884 17.646039877639772 24.967014336785464 68-69 17.576014447351934 39.96351306527107 17.96299708841632 24.497475398960674 70-71 17.38068035233848 38.332657649356875 18.688681679136106 25.59798031916854 72-73 17.512991560092875 34.495264069583165 20.24840600007371 27.74333837025025 74-75 17.646408432536028 32.23933954962592 22.139829727637935 27.974422290200124 76 17.664099067556112 33.15372424722662 21.651863045000553 27.530313640216708 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 2.5 19 5.5 20 8.0 21 10.0 22 16.0 23 35.0 24 53.0 25 58.0 26 89.0 27 155.5 28 226.5 29 262.0 30 350.0 31 505.0 32 685.0 33 798.0 34 932.0 35 1244.5 36 1666.0 37 1909.0 38 5260.5 39 10382.0 40 11221.0 41 8749.0 42 7208.0 43 6213.5 44 4902.0 45 4386.5 46 4188.0 47 4217.5 48 4057.0 49 3627.0 50 3387.0 51 3157.0 52 2702.0 53 2493.5 54 2510.0 55 2566.0 56 2659.0 57 2878.5 58 3061.0 59 3188.5 60 3471.0 61 3751.5 62 3877.0 63 4079.0 64 4317.5 65 4441.5 66 4534.0 67 4539.0 68 4515.0 69 4304.0 70 3836.0 71 3555.0 72 3267.0 73 2610.5 74 1846.0 75 1450.0 76 1136.0 77 582.5 78 239.5 79 136.0 80 78.0 81 12.5 82 3.0 83 1.0 84 2.0 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.78944458777135 #Duplication Level Percentage of deduplicated Percentage of total 1 56.18663823481577 22.918217668521727 2 24.01648083560728 19.59237828474551 3 9.995120805247845 12.230862787012125 4 4.02262500677666 6.563225592451996 5 1.9173428266801598 3.910367449231563 6 1.1258290113305747 2.755316404378432 7 0.7210365578184578 2.058747650462536 8 0.49334080271789216 1.609847786827848 9 0.35961472432549646 1.3201636383739357 >10 1.1167934654932505 6.286072310470645 >50 0.01987820084211287 0.5786311871153208 >100 0.01626398250718326 1.3282718460914753 >500 0.0018071091674648064 0.6737183503482844 >1k 0.0054213275023944196 7.97331662551137 >5k 0.0 0.0 >10k+ 0.0018071091674648064 10.200862418457229 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 13839 10.200862418457229 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 3834 2.8260789444587773 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 3600 2.653595253012936 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 3383 2.4936424280396565 TruSeq Adapter, Index 12 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 914 0.6737183503482844 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 420 0.3095861128515092 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 281 0.20712785169350972 No Hit CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 213 0.1570043858032654 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 211 0.15553016621825821 No Hit CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 174 0.12825710389562525 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT 156 0.11498912763056057 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005159768547525154 0.0 2 0.0 0.0 0.0 0.005159768547525154 0.0 3 0.0 0.0 0.0 0.009582427302546714 0.0 4 0.0 0.0 0.0 0.02801017211513655 0.0 5 0.0 0.0 0.0 0.029484391700143737 0.0 6 0.0 0.0 0.0 0.029484391700143737 0.0 7 0.0 0.0 0.0 0.03022150149264733 0.0 8 0.0 0.0 0.0 0.03022150149264733 0.0 9 0.0 0.0 0.0 0.03906681900269045 0.0 10 0.0 0.0 0.0 0.045700807135222794 0.0 11 0.0 0.0 0.0 0.05086057568274795 0.0 12 0.0 0.0 0.0 0.053071905060258724 0.0 13 0.0 0.0 0.0 0.053071905060258724 0.0 14 0.0 0.0 0.0 0.053071905060258724 0.0 15 0.0 0.0 0.0 0.05454612464526591 0.0 16 0.0 0.0 0.0 0.056757454022776696 0.0 17 0.0 0.0 0.0 0.059705893192791064 0.0 18 0.0 0.0 0.0 0.06633988132532341 0.0 19 0.0 0.0 0.0 0.07665941842037372 0.0 20 0.0 0.0 0.0 0.08034496738289168 0.0 21 0.0 0.0 0.0 0.08255629676040246 0.0 22 0.0 0.0 0.0 0.0891902848929348 0.0 23 0.0 0.0 0.0 0.0899273946854384 0.0 24 0.0 0.0 0.0 0.09656138281797073 0.0 25 0.0 0.0 0.0 0.10614381012051745 0.0 26 0.0 0.0 0.0 0.13120554306563964 0.0 27 0.0 0.0 0.0 0.23734935318615707 0.0 28 0.0 0.0 0.0 0.378874433346847 0.0 29 0.0 0.0 0.0 0.543987026867652 0.0 30 0.0 0.0 0.0 0.7223675966535216 0.0 31 0.0 0.0 0.0 0.9162274720819666 0.0 32 0.0 0.0 0.0 1.1248295433604836 0.0 33 0.0 0.0 0.0 1.419673460361921 0.0 34 0.0 0.0 0.0 1.8177127483138613 0.0 35 0.0 0.0 0.0 2.3631739947665205 0.0 36 0.0 0.0 0.0 3.174731876312977 0.0 37 0.0 0.0 0.0 4.250912173368223 0.0 38 0.0 0.0 0.0 5.324881141045958 0.0 39 0.0 0.0 0.0 6.256587918770501 0.0 40 0.0 0.0 0.0 7.203036892345115 0.0 41 0.0 0.0 0.0 8.178233147827369 0.0 42 0.0 0.0 0.0 9.049496922566616 0.0 43 0.0 0.0 0.0 10.054177569749013 0.0 44 0.0 0.0 0.0 10.990307006228578 0.0 45 0.0 0.0 0.0 11.963291932333322 0.0 46 0.0 0.0 0.0 12.927431540928021 0.0 47 0.0 0.0 0.0 13.796483986289758 0.0 48 0.0 0.0 0.0 14.57044926841853 0.0 49 0.0 0.0 0.0 15.347362989717318 0.0 50 0.0 0.0 0.0 16.133859138318652 0.0 51 0.0 0.0 0.0 16.86728338185973 0.0 52 0.0 0.0 0.0 17.679578373198687 0.0 53 0.0 0.0 0.0 18.366564699812038 0.0 54 0.0 7.371097925035935E-4 0.0 19.045442818707848 0.0 55 0.0 7.371097925035935E-4 0.0 19.75601665868131 0.0 56 0.0 7.371097925035935E-4 0.0 20.47985847491984 0.0 57 0.0 7.371097925035935E-4 0.0 21.121143994397965 0.0 58 0.0 7.371097925035935E-4 0.0 21.83392916374894 0.0 59 0.0 7.371097925035935E-4 0.0 22.481111561567097 0.0 60 0.0 7.371097925035935E-4 0.0 23.106917775402646 0.0 61 0.0 7.371097925035935E-4 0.0 23.779899015958428 0.0 62 0.0 7.371097925035935E-4 0.0 24.340102458261157 0.0 63 0.0 7.371097925035935E-4 0.0 24.92757896288652 0.0 64 0.0 7.371097925035935E-4 0.0 25.5585449452696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACAT 15 0.0022206653 70.0 11 ATAACCC 15 0.0022206653 70.0 28 TTGCATA 15 0.0022206653 70.0 1 TCTTTAT 155 0.0 63.225807 1 GAAAGCT 30 8.340483E-6 58.333332 34 ATTAACG 20 0.006938894 52.499996 18 GCGTACA 20 0.006938894 52.499996 70 TAGTAAC 20 0.006938894 52.499996 34 TTTATAC 210 0.0 51.666664 3 CTTTATA 195 0.0 50.25641 2 TGAAAGC 35 2.0776595E-5 50.0 33 TTGAAAG 35 2.0776595E-5 50.0 32 AAAGCTC 45 9.159048E-5 38.888885 35 AAATACA 40 0.0024119448 34.999996 70 CTATACA 55 2.979655E-4 31.81818 1 AAAAAGC 55 2.979655E-4 31.81818 68 GCTCGTA 55 2.979655E-4 31.81818 38 ATCACAC 45 0.004295207 31.111109 17 AAAAATG 45 0.004295207 31.111109 68 GTCCAAG 45 0.004295207 31.111109 1 >>END_MODULE