FastQCFastQC Report
Wed 25 May 2016
SRR1780213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470492
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC250.002360581235.1718561
TATATCA250.002360581235.1718562
TTGGGAT300.00574902329.32546610
TTAGAAC300.005763978729.3098813
TTAGAGG450.001403470324.42494
GCAGTGT551.5979528E-423.98590933
TATGCAC551.600259E-423.9808123
CGAAACT500.002583729821.99175825
TTAATAC500.00259011821.982412
GTATTAG956.900809E-720.8254411
GTACAGC755.497279E-520.5169161
ATAAAAC550.004461772320.01378840
ATATATC550.00450039419.984011
TCACAGT600.00743978518.3186766
GCTTAGG600.00743978518.3186761
TATGCAG1102.7990536E-617.99325830
TATACCC1102.810446E-617.985613
ACGAAAC750.001288840717.60089324
ATGCAGT1002.4903246E-517.58966831
ATGCACA1053.7923623E-516.7485034
Produced by FastQC (version 0.11.5)