FastQCFastQC Report
Wed 25 May 2016
SRR1780213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780213_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470492
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT215804.5866879776914375No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC64601.3730307847954906No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG55881.1876928831946134No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG40540.8616512076719689Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC12620.2682298530049395No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT4980.10584664563903319Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1550.038.3896261
GTCTTCT5750.037.4933943
TCTTCTG5850.036.85247444
TGAATCG250.002352033535.19787615
CTTTATA1750.033.9480252
CGTCTTC6550.032.91404342
ATGCCGT30400.032.05727844
TTTATAC2100.031.4333553
CCGTCTT7000.030.79814141
TTGGCTG300.00574318729.33156419
CTATCTT300.00574318729.33156425
GCATTAC300.00574318729.33156443
ATACCCT300.00574318729.33156410
CCTCTAT300.00574318729.33156422
GCTTATA407.019456E-427.5041853
TTAGGAA407.0281856E-427.49833938
ACCTAGC407.0281856E-427.4983397
TATCTTA407.0281856E-427.49833926
GCCGTCT8150.026.72231340
TATGCCG37150.026.29181943