FastQCFastQC Report
Wed 25 May 2016
SRR1780212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115373
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAAT305.8573135E-446.66666411
GAAAGTG404.5690205E-543.7525
AAAGTGG350.001251078640.00000426
GTGTTAT400.002410451635.01
TCGTGTA400.002410451635.013
TTACGCT400.002410451635.014
CCAGGGG450.004292565431.11110921
ATGTGTA450.004292565431.1111098
GTATACT450.004292565431.1111093
GGATTGT450.004292565431.1111096
TTCTACA703.463972E-530.0000022
GTATAAG604.9568753E-429.16666612
GGTCAAG500.007183928527.99999828
CTTCTAG500.007183928527.9999981
GGTGAAA500.007183928527.99999822
CGCGTCG500.007183928527.99999866
AAGCTGT500.007183928527.99999862
CTTACGC500.007183928527.99999813
CACCTTG750.001823098423.3333341
AAAAACA800.00265276821.87567
Produced by FastQC (version 0.11.5)