FastQCFastQC Report
Wed 25 May 2016
SRR1780212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115373
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT98978.578263545196883TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG26972.3376353219557435TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC25172.1816196163747152RNA PCR Primer, Index 5 (95% over 21bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG24952.162551030137034TruSeq Adapter, Index 5 (96% over 29bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6510.5642568018513864RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3380.2929628249243757TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT2700.23402355837154276No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1710.14821492030197705No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1580.13694711934334722TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT1420.1230790566250336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAA150.0022198270.014
ATCGTAT150.0022198270.040
ATATCGT150.0022198270.038
TATCGTA150.0022198270.039
TGTGCGG252.3819992E-455.99999610
CGAGTAT200.00693627152.539
GTCAGCA200.00693627152.52
GTAGTCA200.00693627152.542
TCTTTAT850.049.4117661
CTTTATA850.049.4117662
CTATACA506.136543E-848.9999961
TTAATAC451.6725389E-646.6666643
TTGTGCG305.8573135E-446.6666649
AGCTCAA305.8573135E-446.66666447
TCTTAAT551.298904E-744.5454521
CTTAATA503.4536351E-642.02
TAATACA503.4536351E-642.04
TTTATAC1200.040.8333323
CTTAAAC350.001251078640.0000043
GACCAAC350.001251078640.00000464
Produced by FastQC (version 0.11.5)