##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780212_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 115373 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74567706482453 34.0 34.0 34.0 34.0 34.0 2 33.73906373241573 34.0 34.0 34.0 34.0 34.0 3 33.757083546410335 34.0 34.0 34.0 34.0 34.0 4 33.7796018132492 34.0 34.0 34.0 34.0 34.0 5 33.780225876071526 34.0 34.0 34.0 34.0 34.0 6 37.541235817738986 38.0 38.0 38.0 37.0 38.0 7 37.543238019293945 38.0 38.0 38.0 37.0 38.0 8 37.46045435240481 38.0 38.0 38.0 37.0 38.0 9 37.48305062709646 38.0 38.0 38.0 37.0 38.0 10-11 37.430672687717234 38.0 38.0 38.0 37.0 38.0 12-13 37.42580586445702 38.0 38.0 38.0 37.0 38.0 14-15 37.36208211626637 38.0 38.0 38.0 37.0 38.0 16-17 37.37980723392822 38.0 38.0 38.0 36.5 38.0 18-19 37.31982352890191 38.0 38.0 38.0 36.0 38.0 20-21 37.35726296447176 38.0 38.0 38.0 36.5 38.0 22-23 37.36051762544096 38.0 38.0 38.0 37.0 38.0 24-25 37.335568113856795 38.0 38.0 38.0 36.5 38.0 26-27 37.23012749950162 38.0 38.0 38.0 36.0 38.0 28-29 37.23286210811888 38.0 38.0 38.0 36.0 38.0 30-31 37.2344222651747 38.0 38.0 38.0 36.0 38.0 32-33 37.07603165385315 38.0 38.0 38.0 36.0 38.0 34-35 37.120643478110125 38.0 38.0 38.0 36.0 38.0 36-37 37.14322241772338 38.0 38.0 38.0 36.0 38.0 38-39 36.31933814670677 38.0 37.0 38.0 34.0 38.0 40-41 36.724974647447844 38.0 37.0 38.0 34.5 38.0 42-43 37.08583897445676 38.0 38.0 38.0 36.0 38.0 44-45 37.19433489637957 38.0 38.0 38.0 36.0 38.0 46-47 37.125588309223126 38.0 38.0 38.0 36.0 38.0 48-49 37.14111620569804 38.0 38.0 38.0 36.0 38.0 50-51 37.000940428003084 38.0 38.0 38.0 36.0 38.0 52-53 36.98004299099443 38.0 38.0 38.0 36.0 38.0 54-55 36.96227453563659 38.0 38.0 38.0 35.5 38.0 56-57 36.99633796468844 38.0 38.0 38.0 36.0 38.0 58-59 36.8988844877051 38.0 38.0 38.0 35.5 38.0 60-61 36.88557114749551 38.0 38.0 38.0 35.0 38.0 62-63 36.85786102467648 38.0 38.0 38.0 35.0 38.0 64-65 36.74824699019701 38.0 38.0 38.0 34.5 38.0 66-67 36.36398030735094 38.0 37.5 38.0 34.0 38.0 68-69 36.22022050219722 38.0 37.0 38.0 34.0 38.0 70-71 35.367668345280094 38.0 36.5 38.0 28.5 38.0 72-73 33.860240264186594 38.0 35.5 38.0 21.5 38.0 74-75 33.40419768923405 38.0 34.0 38.0 15.0 38.0 76 32.7672765725083 38.0 32.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.006492186465337113 1101 2 -0.05941061835344641 1101 3 0.11521490949908753 1101 4 0.06558138838265393 1101 5 -0.04202125453807071 1101 6 -0.10397264428441844 1101 7 0.11721467107443573 1101 8 -0.1960117449805452 1101 9 0.10896296497208624 1101 10-11 0.11162985962801741 1101 12-13 0.06558669969015796 1101 14-15 0.23589065668106457 1101 16-17 0.24137229440010088 1101 18-19 0.0395071647472065 1101 20-21 0.20376567502186305 1101 22-23 -0.05959215465031775 1101 24-25 0.03427487328806933 1101 26-27 0.1316853971258638 1101 28-29 -0.08038258090307693 1101 30-31 -0.02560722633641177 1101 32-33 -0.4085307612212006 1101 34-35 -0.1248687936122792 1101 36-37 0.14072807616480532 1101 38-39 0.1930349220481773 1101 40-41 0.17635831693328896 1101 42-43 0.021211670847257835 1101 44-45 0.04784450073959334 1101 46-47 -0.06925198344797678 1101 48-49 0.052669276529051956 1101 50-51 0.21926161413828993 1101 52-53 0.031045810314765276 1101 54-55 0.03134949595735037 1101 56-57 -0.02592931081669292 1101 58-59 -0.15193487709355225 1101 60-61 -0.012751689164780089 1101 62-63 -0.02283767800886949 1101 64-65 -0.40631306095392716 1101 66-67 -0.402301317425767 1101 68-69 -0.6517167957572596 1101 70-71 -0.952344642112962 1101 72-73 -1.1150059928492837 1101 74-75 -0.7991970009959743 1101 76 -1.113215408388374 1102 1 -0.03777500717051652 1102 2 -0.06363310254592136 1102 3 -0.07134601644668237 1102 4 0.07772370967725806 1102 5 -0.04388600644033147 1102 6 0.019165283054555005 1102 7 -0.04039973252646689 1102 8 -0.16492182964456248 1102 9 0.004408254865992944 1102 10-11 -0.02265371126470228 1102 12-13 -0.15699394547112888 1102 14-15 0.29120627129901067 1102 16-17 0.09112580420612204 1102 18-19 -0.07921537303009529 1102 20-21 -0.05251982492455198 1102 22-23 0.04718096720157661 1102 24-25 0.0010844328208037268 1102 26-27 0.14608899802608732 1102 28-29 0.02960027195299375 1102 30-31 -0.018780519659742367 1102 32-33 0.17834095670828276 1102 34-35 0.20430814347913895 1102 36-37 -0.022839601468888304 1102 38-39 0.3951515226268967 1102 40-41 0.2740059431255446 1102 42-43 0.10640082527871897 1102 44-45 0.18810813807749582 1102 46-47 0.11993459891189673 1102 48-49 0.033207268169817894 1102 50-51 0.07955525897807547 1102 52-53 0.09277016997610588 1102 54-55 -0.034586630931521256 1102 56-57 0.21658488916186514 1102 58-59 0.34790972691458677 1102 60-61 0.28342771282856916 1102 62-63 0.15054673952408848 1102 64-65 0.1942495797062378 1102 66-67 0.30896757122496865 1102 68-69 0.26477125482680464 1102 70-71 0.7038444051488568 1102 72-73 0.361679249889967 1102 74-75 0.5252323206201339 1102 76 0.5368756858137473 1103 1 -0.20165862513383104 1103 2 0.09253388037627985 1103 3 -0.010118944972937527 1103 4 -0.07500662977034267 1103 5 -0.027820225289161726 1103 6 -0.01941789215101153 1103 7 0.19807802416760723 1103 8 0.07747749567695905 1103 9 -0.04138554652553239 1103 10-11 0.10558790796363127 1103 12-13 0.09336447746792942 1103 14-15 -0.033630330008186604 1103 16-17 -0.05143164888264096 1103 18-19 0.19037138640486262 1103 20-21 0.1049121801355355 1103 22-23 0.11161276142463805 1103 24-25 -0.03542199770839716 1103 26-27 0.08205779414668513 1103 28-29 0.08373267760342884 1103 30-31 -0.11889648002238573 1103 32-33 0.09025324850671979 1103 34-35 -0.17217298660730762 1103 36-37 0.06882179427219626 1103 38-39 0.07803155636081982 1103 40-41 -0.06970057173846556 1103 42-43 -0.00691353272254247 1103 44-45 -0.02608119356913363 1103 46-47 -0.03321741458167082 1103 48-49 0.10453343304437368 1103 50-51 -0.14513375641303128 1103 52-53 -0.22034389285332878 1103 54-55 -0.21690794655453516 1103 56-57 -0.1612245141693549 1103 58-59 0.18584984920006065 1103 60-61 0.30172836642334033 1103 62-63 0.04079552721120194 1103 64-65 0.08365501930143182 1103 66-67 -0.29846443383512167 1103 68-69 -0.22431792923391214 1103 70-71 -0.19341471355238582 1103 72-73 0.031276551176077305 1103 74-75 -0.27637004075451443 1103 76 -0.4564829479623427 1104 1 0.10553091734983155 1104 2 -0.054524943153133165 1104 3 -0.08201436980953503 1104 4 0.042640429053186324 1104 5 0.011395460985347938 1104 6 -0.06190155228173211 1104 7 -0.10257557060363354 1104 8 -0.13167283112042583 1104 9 -0.10347704979351136 1104 10-11 0.07617614325774724 1104 12-13 -0.21382506501572607 1104 14-15 0.023559212475468883 1104 16-17 -0.08411138744472879 1104 18-19 0.06455439294080634 1104 20-21 0.0820363631420733 1104 22-23 -0.017472205895614934 1104 24-25 -0.04849389313322661 1104 26-27 0.20624080068262884 1104 28-29 -0.1989470609586661 1104 30-31 -0.16301412708121177 1104 32-33 -0.12706701293119238 1104 34-35 -0.11334945719553957 1104 36-37 0.08679565048134918 1104 38-39 -0.23079197305094112 1104 40-41 -0.07296854559466937 1104 42-43 -0.09351483991208198 1104 44-45 -0.03425112820966092 1104 46-47 -0.35347885249016997 1104 48-49 -0.1046168937530112 1104 50-51 -0.29382656687054975 1104 52-53 -0.3036772261866574 1104 54-55 -0.11233278315584272 1104 56-57 -0.15632255338503853 1104 58-59 0.06330082959222239 1104 60-61 0.001074771652099571 1104 62-63 -0.11116525710252745 1104 64-65 -0.37549530749595306 1104 66-67 -0.31807227697237295 1104 68-69 -0.08216106648881549 1104 70-71 -0.2996238638791837 1104 72-73 -0.5063051481703269 1104 74-75 -0.3695072956564758 1104 76 -0.35844373227607207 1105 1 0.02887448267798476 1105 2 -0.04155745583871351 1105 3 -0.1577728947317496 1105 4 0.025172952179580932 1105 5 0.12484531376991725 1105 6 -0.05957325141931591 1105 7 -0.07068933368074681 1105 8 -0.05910919437712181 1105 9 0.034790610838371094 1105 10-11 0.05144708230429984 1105 12-13 -0.07019604791367584 1105 14-15 0.003826079605715904 1105 16-17 -0.06512999297000732 1105 18-19 0.0190428976078465 1105 20-21 0.0021745994802628843 1105 22-23 -0.0032570416616621856 1105 24-25 -0.038841363324713996 1105 26-27 0.1091725804232837 1105 28-29 0.16442970614860286 1105 30-31 -0.0879446956877814 1105 32-33 0.10714648077299671 1105 34-35 -0.07196208222426037 1105 36-37 -0.011374075342949652 1105 38-39 0.04620274389866097 1105 40-41 -0.25433065380323683 1105 42-43 -0.050530020851340396 1105 44-45 -0.13008211236051181 1105 46-47 -0.1287925988619847 1105 48-49 -0.3489631945736633 1105 50-51 -0.3779011977878781 1105 52-53 -0.12812542932307736 1105 54-55 -0.18245327011526769 1105 56-57 -0.1593503885872849 1105 58-59 0.2099472410854588 1105 60-61 -0.011939699312002006 1105 62-63 -0.32688807445097723 1105 64-65 -0.08608124581164134 1105 66-67 -0.5359824442341647 1105 68-69 -0.19330872043857283 1105 70-71 -0.1262802985510305 1105 72-73 -0.07177947421597963 1105 74-75 -0.04116467000132218 1105 76 -0.037482760027742756 1106 1 0.11150125420252266 1106 2 -7.919114789203263E-4 1106 3 -0.09496057393221236 1106 4 0.005184421008941342 1106 5 0.050799684213103546 1106 6 -0.16063207012989267 1106 7 -0.030742991418108545 1106 8 -0.0610343193054419 1106 9 0.02686483054836941 1106 10-11 0.014618828023962749 1106 12-13 0.03674054584400466 1106 14-15 0.07229601488874948 1106 16-17 -0.08175467552919002 1106 18-19 0.0794488122469943 1106 20-21 0.1298933264402038 1106 22-23 0.06846922195757088 1106 24-25 0.13998021445852515 1106 26-27 0.09095045377463862 1106 28-29 -9.518372281505094E-4 1106 30-31 -0.09067878268704277 1106 32-33 0.21512755720455345 1106 34-35 -0.2000136100361658 1106 36-37 -0.2224363756624399 1106 38-39 -0.035876186223390505 1106 40-41 -0.047830285162291375 1106 42-43 0.045782545708824784 1106 44-45 0.015899701352935836 1106 46-47 -0.22803893293260558 1106 48-49 -0.1716417806308428 1106 50-51 -0.0859017302692422 1106 52-53 0.04118828412002529 1106 54-55 0.1790196552554164 1106 56-57 0.3113119363082717 1106 58-59 0.06600319258869547 1106 60-61 0.314517456418308 1106 62-63 0.3109375583826477 1106 64-65 0.40633601477654935 1106 66-67 0.20860570191148753 1106 68-69 0.15765793803607409 1106 70-71 -0.06769198856495962 1106 72-73 -0.2054630164458402 1106 74-75 0.10262065808912268 1106 76 -0.3109324200588759 1107 1 -0.18192823297696492 1107 2 -0.13853229609430429 1107 3 -0.05521698418861831 1107 4 0.008469677419199684 1107 5 -0.1604182645048482 1107 6 -0.14797181371963575 1107 7 0.012926880376760153 1107 8 0.04465478325865746 1107 9 -0.039240938682397086 1107 10-11 -0.22532508223244463 1107 12-13 0.04839919969015938 1107 14-15 0.10995627129900498 1107 16-17 0.010833465496439487 1107 18-19 -0.20885246980429173 1107 20-21 -0.025755712021329202 1107 22-23 -0.08185129086294296 1107 24-25 -0.08117363169532155 1107 26-27 -0.33364890519971624 1107 28-29 0.013471239694929693 1107 30-31 -0.06147205191781069 1107 32-33 0.04830063412762797 1107 34-35 -0.09488540490796282 1107 36-37 -0.1677488756624399 1107 38-39 0.014203941981733692 1107 40-41 -0.12307066977767533 1107 42-43 -0.24692578762450523 1107 44-45 -0.11859549095476041 1107 46-47 -0.21549886883003921 1107 48-49 -0.06699434473340204 1107 50-51 -0.11454756360257079 1107 52-53 0.06943347642771869 1107 54-55 0.020806514229768425 1107 56-57 -0.02787075599941602 1107 58-59 0.2673653720758722 1107 60-61 -0.16188478717142374 1107 62-63 -0.006610518540426824 1107 64-65 0.025045950673977302 1107 66-67 -0.02248404167825413 1107 68-69 0.055093835471971886 1107 70-71 -0.36208301420597877 1107 72-73 0.10936070150287236 1107 74-75 -0.33031203421856503 1107 76 -0.7680638303152847 1108 1 -0.07747561859788021 1108 2 -0.047988995440711335 1108 3 0.03890066287020488 1108 4 0.045908402909390134 1108 5 -0.15200323182510544 1108 6 -0.35601919934055104 1108 7 -0.05682393661670204 1108 8 -0.04670551085898467 1108 9 -0.034849598813117666 1108 10-11 -0.1460460789644742 1108 12-13 -0.13375970553859418 1108 14-15 -0.09572183327615846 1108 16-17 -0.1592747861375443 1108 18-19 0.04821452365976597 1108 20-21 -0.15162376757688634 1108 22-23 -0.19230880720280652 1108 24-25 -0.05339585391754298 1108 26-27 0.12617544120551116 1108 28-29 0.07719672989100701 1108 30-31 0.14090744154624701 1108 32-33 -0.06007354887890415 1108 34-35 -0.015310241509268963 1108 36-37 0.16522702303036851 1108 38-39 0.10580933413859839 1108 40-41 -0.22002736912408238 1108 42-43 -0.24710961115391683 1108 44-45 -0.10941452690246933 1108 46-47 -0.04955728386271119 1108 48-49 0.06368375984175856 1108 50-51 0.031336831822265765 1108 52-53 -0.10923278174221451 1108 54-55 -0.15971840407087967 1108 56-57 -0.24292386057458515 1108 58-59 -0.09192792857771792 1108 60-61 -0.18356575122371765 1108 62-63 -0.14384499566461528 1108 64-65 0.1865961957720188 1108 66-67 -0.005980773704401088 1108 68-69 0.07143370475301936 1108 70-71 0.4732519531142785 1108 72-73 0.4969628256858769 1108 74-75 0.49323780238273685 1108 76 0.8278307775278506 1109 1 0.09448305734561302 1109 2 -0.02814923157817617 1109 3 0.028653983553311946 1109 4 -0.209373064516285 1109 5 0.12708173549515323 1109 6 -0.28083471694544215 1109 7 0.07572929973159859 1109 8 -0.08427666835424219 1109 9 -0.0826885193275615 1109 10-11 -0.1694279048130909 1109 12-13 0.017199602915965784 1109 14-15 -0.08298727708808684 1109 16-17 -0.00726129256806729 1109 18-19 -0.014699243997839062 1109 20-21 -0.07187466363423312 1109 22-23 -0.039915806991970726 1109 24-25 -0.09085105105015856 1109 26-27 -0.2845561632642344 1109 28-29 -0.24298037320829735 1109 30-31 -0.12039342288554877 1109 32-33 -0.02488484974333005 1109 34-35 -0.22794992103699485 1109 36-37 -0.03251702082373242 1109 38-39 -0.6645258967279446 1109 40-41 -0.013090831067998465 1109 42-43 -0.022631432785793493 1109 44-45 -0.029633797406368956 1109 46-47 -0.07845249786229402 1109 48-49 -0.10066369957210952 1109 50-51 -0.3768963539251473 1109 52-53 -0.0572298300238927 1109 54-55 -0.10716727609280952 1109 56-57 -0.21889898180587153 1109 58-59 -0.19725156340799543 1109 60-61 -0.24883035168755896 1109 62-63 -0.6268726153146247 1109 64-65 -0.16381493642279565 1109 66-67 -0.0829679126459979 1109 68-69 0.009932545149389682 1109 70-71 -0.012284627109210078 1109 72-73 -0.8576755888197098 1109 74-75 -0.24896122776695506 1109 76 -0.29538237870237793 1110 1 -0.11976340647854045 1110 2 -0.12002874719841117 1110 3 0.0018808076094884996 1110 4 0.05970193293970283 1110 5 0.07566217713553414 1110 6 -0.06234484211080371 1110 7 -0.13331720479673947 1110 8 -0.03575728298739733 1110 9 -0.08227130145841954 1110 10-11 -0.26097669895168707 1110 12-13 -0.09845115519942738 1110 14-15 -0.201065022076385 1110 16-17 -0.10220379791049794 1110 18-19 0.041482703697283796 1110 20-21 0.057717276937658823 1110 22-23 -0.16103110158849177 1110 24-25 -0.09142599762591885 1110 26-27 -4.7658658773030993E-4 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-0.02688556527481012 2115 52-53 0.014223401176877815 2115 54-55 0.10321140553411823 2115 56-57 -0.01770144161815068 2115 58-59 0.16731311455079378 2115 60-61 0.13509995363124716 2115 62-63 0.12469173898465158 2115 64-65 0.1576598720786606 2115 66-67 0.11572666066287951 2115 68-69 0.07098012309739232 2115 70-71 -0.41252197741668795 2115 72-73 -0.04980526839678845 2115 74-75 -0.3102137900714155 2115 76 -0.04894802763969608 2116 1 -0.0118466178566905 2116 2 0.029970710778542298 2116 3 -0.16758811820923825 2116 4 -0.16452216106877415 2116 5 -0.047832353851923415 2116 6 0.06871633061025761 2116 7 -0.09064915055107292 2116 8 0.153213632055909 2116 9 0.03340725719389326 2116 10-11 -0.019993252335538614 2116 12-13 -0.20417275048166061 2116 14-15 -0.027178608426076778 2116 16-17 -0.21843522007056748 2116 18-19 -0.10251655915136837 2116 20-21 -0.21032938040620763 2116 22-23 -0.08288221869798917 2116 24-25 -0.02361349423827619 2116 26-27 0.08141767555629542 2116 28-29 -0.023470341061077704 2116 30-31 -0.025287558790658693 2116 32-33 -0.3063170634668637 2116 34-35 -0.004765129993870687 2116 36-37 -0.16680922789611685 2116 38-39 0.012217258132935171 2116 40-41 -0.16182711307664732 2116 42-43 -0.1842432274870518 2116 44-45 -0.07675163356644532 2116 46-47 -0.029915750273332264 2116 48-49 -0.10967046157189486 2116 50-51 -0.05218179899775777 2116 52-53 -0.015859693675373876 2116 54-55 -0.06509876927538016 2116 56-57 -0.054105721635160364 2116 58-59 -0.2008154698485214 2116 60-61 -0.10540390229170526 2116 62-63 0.02890279761076897 2116 64-65 -0.3628782331748184 2116 66-67 -0.23751840593942575 2116 68-69 -0.145077985833872 2116 70-71 -0.23744813448089985 2116 72-73 -0.08072735691636979 2116 74-75 0.3383627939601226 2116 76 0.3028661525025882 2117 1 0.10696065591191939 2117 2 0.14584270390569287 2117 3 0.06422746025582171 2117 4 0.1331224551969754 2117 5 -0.002084931171509652 2117 6 0.1235559640581414 2117 7 0.22994076926564588 2117 8 -0.30569243896356113 2117 9 -0.006254827571282817 2117 10-11 0.23804297332311108 2117 12-13 0.22878114413460082 2117 14-15 0.2332201601878907 2117 16-17 0.05927096549644517 2117 18-19 0.09427253019570969 2117 20-21 0.1278901213119994 2117 22-23 0.14939870913705988 2117 24-25 0.14452081274912132 2117 26-27 0.17294831702250235 2117 28-29 -0.4031954269717417 2117 30-31 0.048076559193305 2117 32-33 0.03510618968319079 2117 34-35 0.048517372869817166 2117 36-37 0.17513306878200297 2117 38-39 0.32323171975951226 2117 40-41 0.3557487746667647 2117 42-43 0.25064365681993905 2117 44-45 0.06664756460079957 2117 46-47 0.051341408947742195 2117 48-49 0.11842232193326652 2117 50-51 0.3116677141752078 2117 52-53 0.14148208753883296 2117 54-55 -0.048811541325783026 2117 56-57 -0.26814853377720027 2117 58-59 0.20555981652032074 2117 60-61 -0.015877842726986557 2117 62-63 0.2895700370151246 2117 64-65 -0.02755821599269126 2117 66-67 0.029252069432857297 2117 68-69 0.0012743910275290204 2117 70-71 0.5351392080162398 2117 72-73 -0.0916809651637962 2117 74-75 0.04572963244810069 2117 76 -0.6934110525375061 2118 1 -0.08517300649612736 2118 2 0.0375021115712002 2118 3 -0.13500792495516833 2118 4 -0.05896281387000357 2118 5 0.11209915709794416 2118 6 0.18506390056607813 2118 7 0.28835153194470564 2118 8 0.07114650799733369 2118 9 0.17662360835593205 2118 10-11 0.12025963031110365 2118 12-13 0.15966335474242044 2118 14-15 0.029708013459284643 2118 16-17 0.20713681915497517 2118 18-19 0.05218847096226398 2118 20-21 0.044175690417695535 2118 22-23 0.12860167080953033 2118 24-25 -0.02281126235733666 2118 26-27 0.14813463138564487 2118 28-29 0.15197298185520225 2118 30-31 -0.02236599791083904 2118 32-33 0.03900185363983155 2118 34-35 0.158860239691343 2118 36-37 0.08745038391944604 2118 38-39 -0.1378539848475313 2118 40-41 0.24264143823626227 2118 42-43 0.23270182561591213 2118 44-45 0.168539744236881 2118 46-47 0.16881624441037957 2118 48-49 0.13337586432582782 2118 50-51 0.2412614520768699 2118 52-53 0.0878078231176147 2118 54-55 0.27841213269667264 2118 56-57 0.3354676586347267 2118 58-59 0.14488061597831603 2118 60-61 0.02541377554634039 2118 62-63 0.2986921818080006 2118 64-65 0.2901864123464506 2118 66-67 0.19955428584787427 2118 68-69 0.2319231037646503 2118 70-71 0.20914956419123598 2118 72-73 0.06083064923806347 2118 74-75 0.6448512933075676 2118 76 0.3241073369321015 2119 1 0.22449907198757302 2119 2 0.1438480230546304 2119 3 0.1809532285773372 2119 4 0.03819042210005108 2119 5 -0.04951400918569959 2119 6 0.006239179188163746 2119 7 0.13981074562498463 2119 8 0.023799286804759845 2119 9 0.02181845848072328 2119 10-11 0.25640142485975304 2119 12-13 0.11877818905185933 2119 14-15 -0.34460933153787465 2119 16-17 0.16787025627658636 2119 18-19 0.20076632452195042 2119 20-21 0.10428639790774952 2119 22-23 0.08446076587465257 2119 24-25 0.1546419711415581 2119 26-27 0.40617822245985025 2119 28-29 0.297159182957337 2119 30-31 0.13743087361410744 2119 32-33 -0.008481280765984422 2119 34-35 0.11770324757431183 2119 36-37 0.11488631937302074 2119 38-39 0.551992062549111 2119 40-41 0.4434408550450115 2119 42-43 0.21200594996414424 2119 44-45 0.2562161225204207 2119 46-47 0.2740068935813156 2119 48-49 0.1725003361176647 2119 50-51 0.2292578441988482 2119 52-53 0.36159881330715393 2119 54-55 0.24189472344963292 2119 56-57 0.2794652369083792 2119 58-59 0.3137527834233964 2119 60-61 0.26640643623282045 2119 62-63 0.3417272474170119 2119 64-65 0.1768965712413504 2119 66-67 0.39719680938557644 2119 68-69 0.3902002184506941 2119 70-71 0.833528687921671 2119 72-73 1.1711736447652825 2119 74-75 1.2751179303204339 2119 76 0.1546976945074121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 6.0 23 14.0 24 29.0 25 42.0 26 107.0 27 197.0 28 366.0 29 526.0 30 933.0 31 1459.0 32 2315.0 33 3975.0 34 6743.0 35 11528.0 36 16707.0 37 70422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.853743943556985 13.443353297565288 11.33627451830151 37.36662824057622 2 20.619209000372702 17.315143057734478 34.96311962070848 27.102528321184334 3 19.95354198989365 15.257469251904693 32.54574293812244 32.24324582007922 4 15.391816109488355 23.47516316642542 29.47916756953533 31.653853154550895 5 16.37731531640852 27.395491146108707 38.762102051606526 17.465091485876243 6 30.13963405649502 35.90181411595434 15.443821344682032 18.514730482868607 7 28.367122290310558 23.30527939812608 19.719518431522108 28.60807988004126 8 24.35665190295823 36.19304343303892 14.40371664080851 25.04658802319433 9 21.954010037010395 15.475024485798237 14.61693810510258 47.954027372088795 10-11 26.722890104270498 25.8791051632531 22.064521161796954 25.333483570679448 12-13 25.909441550449415 16.97970929073527 25.06305634767233 32.04779281114299 14-15 22.71501997867785 16.11338874780061 18.939006526657018 42.23258474686452 16-17 29.597912856560892 23.68838463071949 17.185996723670183 29.52770578904943 18-19 32.279649484714795 21.810562263267837 17.47679266379482 28.43299558822255 20-21 25.657216159760083 19.300442911253064 17.540499076907075 37.50184185207978 22-23 23.835299420141627 25.082124933910016 17.31817669645411 33.76439894949425 24-25 29.657285500073677 25.221238938053098 17.102354970400356 28.019120591472873 26-27 30.4802683470136 28.165602003935064 17.705182321687047 23.64894732736429 28-29 26.071524533469702 25.704454248394338 19.275307047576124 28.948714170559835 30-31 24.48579823702253 26.60154455548525 17.628041222816428 31.28461598467579 32-33 22.608409246530815 31.78776663517461 19.248871053019336 26.35495306527524 34-35 29.08479453598329 22.609276000450713 24.314180960883398 23.991748502682604 36-37 31.877475665883697 21.915439487575085 22.591074168132923 23.61601067840829 38-39 23.831832404462048 21.996914356045174 26.326783562878664 27.844469676614114 40-41 23.189134372860202 17.111022509599298 27.50946928657485 32.190373830965655 42-43 28.844703700172484 16.78815667443856 26.41215882398828 27.954980801400676 44-45 22.46236121102858 23.01925060456086 29.930312984840473 24.58807519957009 46-47 26.69385384795403 19.102823017517096 26.639248351000667 27.56407478352821 48-49 26.1603668102589 17.26833834606017 22.397788043996428 34.1735067996845 50-51 23.609076647049136 17.924037686460437 27.333951617796192 31.13293404869423 52-53 19.130559142953725 18.22913506626334 36.027926811299004 26.612378979483935 54-55 20.114758218994044 19.220701550622763 30.17170395153112 30.49283627885207 56-57 24.678217607239127 20.524299446144244 23.911573765092353 30.885909181524273 58-59 20.654745911088384 20.805127716190096 30.940948055437577 27.59917831728394 60-61 25.02015202863755 25.47173082090264 24.3215483692025 25.186568781257314 62-63 19.803160184791935 35.10960103317068 19.464692779073093 25.6225460029643 64-65 18.891335061062815 37.44810310904631 18.301075641614588 25.359486188276286 66-67 19.232402728541338 36.940185311988074 17.840829310150554 25.986582649320034 68-69 18.516897367668346 37.43076803064842 18.030215041647526 26.022119560035712 70-71 18.623508099815382 36.360326939578584 18.352214122888373 26.663950837717664 72-73 19.128825635113937 33.52344135976355 18.911703778180335 28.43602922694218 74-75 19.192965425186134 32.93231518639543 19.539233616184028 28.335485772234403 76 19.491562150589825 32.979986651989634 19.253204822618812 28.275246374801732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 3.5 16 4.0 17 1.0 18 4.0 19 6.0 20 13.0 21 21.0 22 24.5 23 36.5 24 59.0 25 73.0 26 89.0 27 149.5 28 248.0 29 302.0 30 366.0 31 490.5 32 621.0 33 691.0 34 824.5 35 1087.5 36 1333.5 37 1450.0 38 1778.5 39 4661.0 40 8489.5 41 8948.0 42 8132.0 43 7000.0 44 5443.0 45 4735.0 46 4452.0 47 4196.0 48 3800.5 49 3555.0 50 3449.0 51 3242.0 52 2883.0 53 2557.0 54 2383.0 55 2379.0 56 2256.5 57 2308.0 58 2478.0 59 2451.5 60 2533.5 61 2663.5 62 2685.0 63 2859.5 64 3107.0 65 3217.0 66 3313.5 67 3373.0 68 3335.5 69 3322.5 70 3196.5 71 3046.0 72 2934.0 73 2625.0 74 2133.0 75 1838.0 76 1626.0 77 1157.5 78 711.0 79 521.0 80 368.0 81 148.5 82 54.5 83 27.0 84 15.0 85 2.0 86 1.0 87 1.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 115373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.321531034124106 #Duplication Level Percentage of deduplicated Percentage of total 1 63.399103139013455 30.63541729867473 2 22.154260089686098 21.410555329236477 3 7.7596412556053815 11.248732372392155 4 2.921973094170404 5.647768542033231 5 1.3560538116591927 3.276329817201598 6 0.7318385650224215 2.1218135959019873 7 0.4789237668161435 1.6199630762830126 8 0.29417040358744395 1.1371811429017187 9 0.22242152466367712 0.9672973746023766 >10 0.6439461883408072 4.417844729702789 >50 0.017937219730941704 0.6639335026392656 >100 0.010762331838565023 1.0288369029148934 >500 0.0017937219730941704 0.5642568018513864 >1k 0.0053811659192825115 6.681805968467493 >5k 0.0017937219730941704 8.578263545196883 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 9897 8.578263545196883 TruSeq Adapter, Index 5 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 2697 2.3376353219557435 TruSeq Adapter, Index 5 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 2517 2.1816196163747152 RNA PCR Primer, Index 5 (95% over 21bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 2495 2.162551030137034 TruSeq Adapter, Index 5 (96% over 29bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 651 0.5642568018513864 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 338 0.2929628249243757 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 270 0.23402355837154276 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 171 0.14821492030197705 No Hit CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 158 0.13694711934334722 TruSeq Adapter, Index 5 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT 142 0.1230790566250336 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002600261759683808 0.0 2 0.0 0.0 0.0 0.004333769599473013 0.0 3 0.0 0.0 0.0 0.006067277439262219 0.0 4 0.0 0.0 0.0 0.014734816638208246 0.0 5 0.0 0.0 0.0 0.014734816638208246 0.0 6 0.0 0.0 0.0 0.014734816638208246 0.0 7 0.0 0.0 0.0 0.015601570558102849 0.0 8 0.0 0.0 0.0 0.015601570558102849 0.0 9 0.0 0.0 0.0 0.01906858623768126 0.0 10 0.0 0.0 0.0 0.02253560191725967 0.0 11 0.0 0.0 0.0 0.024269109757048875 0.0 12 0.0 0.0 0.0 0.024269109757048875 0.0 13 0.0 0.0 0.0 0.027736125436627287 0.0 14 0.0 0.0 0.0 0.027736125436627287 0.0 15 0.0 0.0 0.0 0.02860287935652189 0.0 16 0.0 0.0 0.0 0.029469633276416492 0.0 17 0.0 0.0 0.0 0.034670156795784106 0.0 18 0.0 0.0 0.0 0.03813717247536252 0.0 19 0.0 0.0 0.0 0.03987068031515172 0.0 20 0.0 0.0 0.0 0.04247094207483553 0.0 21 0.0 0.0 0.0 0.04767146559420315 0.0 22 0.0 0.0 0.0 0.055472250873254574 0.0 23 0.0 0.0 0.0 0.058939266552832985 0.0 24 0.0 0.0 0.0 0.06847355967167361 0.0 25 0.0 0.0 0.0 0.08320837630988186 0.0 26 0.0 0.0 0.0 0.09880994686798471 0.0 27 0.0 0.0 0.0 0.18201832317786656 0.0 28 0.0 0.0 0.0 0.270427223007116 0.0 29 0.0 0.0 0.0 0.39957355707141184 0.0 30 0.0 0.0 0.0 0.5252528754561292 0.0 31 0.0 0.0 0.0 0.6396643928822168 0.0 32 0.0 0.0 0.0 0.8598198885354459 0.0 33 0.0 0.0 0.0 1.084309153788148 0.0 34 0.0 0.0 0.0 1.409341873748624 0.0 35 0.0 0.0 0.0 1.8167162160990873 0.0 36 0.0 0.0 0.0 2.393107572828998 0.0 37 0.0 0.0 0.0 3.1957217026514004 0.0 38 0.0 0.0 0.0 4.046007298068005 0.0 39 0.0 0.0 0.0 4.847754673970513 0.0 40 0.0 0.0 0.0 5.697173515467224 0.0 41 0.0 0.0 0.0 6.660137120470127 0.0 42 0.0 0.0 0.0 7.685507007705442 0.0 43 0.0 0.0 0.0 8.751614329175803 0.0 44 0.0 0.0 0.0 9.737980290015862 0.0 45 0.0 0.0 0.0 10.739081067494128 0.0 46 0.0 0.0 0.0 11.624036819706516 0.0 47 0.0 0.0 0.0 12.414516394650395 0.0 48 0.0 0.0 0.0 13.178993351997434 0.0 49 0.0 0.0 0.0 13.950404340703631 0.0 50 0.0 0.0 0.0 14.633406429580578 0.0 51 0.0 0.0 0.0 15.423019250604561 0.0 52 0.0 0.0 0.0 16.239501443145276 0.0 53 0.0 0.0 0.0 17.14179227375556 0.0 54 0.0 0.0 0.0 18.060551428843837 0.0 55 0.0 0.0 0.0 18.860565296906554 0.0 56 0.0 0.0 0.0 19.58257131217876 0.0 57 0.0 0.0 0.0 20.190165810024876 0.0 58 0.0 0.0 0.0 20.84803203522488 0.0 59 0.0 0.0 0.0 21.49029668986678 0.0 60 0.0 0.0 0.0 22.120426789630155 0.0 61 0.0 0.0 0.0 22.683816837561647 0.0 62 0.0 0.0 0.0 23.302679136366393 0.0 63 0.0 0.0 0.0 23.944943791008296 0.0 64 0.0 0.0 0.0 24.646147712203028 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATAA 15 0.00221982 70.0 14 ATCGTAT 15 0.00221982 70.0 40 ATATCGT 15 0.00221982 70.0 38 TATCGTA 15 0.00221982 70.0 39 TGTGCGG 25 2.3819992E-4 55.999996 10 CGAGTAT 20 0.006936271 52.5 39 GTCAGCA 20 0.006936271 52.5 2 GTAGTCA 20 0.006936271 52.5 42 TCTTTAT 85 0.0 49.411766 1 CTTTATA 85 0.0 49.411766 2 CTATACA 50 6.136543E-8 48.999996 1 TTAATAC 45 1.6725389E-6 46.666664 3 TTGTGCG 30 5.8573135E-4 46.666664 9 AGCTCAA 30 5.8573135E-4 46.666664 47 TCTTAAT 55 1.298904E-7 44.545452 1 CTTAATA 50 3.4536351E-6 42.0 2 TAATACA 50 3.4536351E-6 42.0 4 TTTATAC 120 0.0 40.833332 3 CTTAAAC 35 0.0012510786 40.000004 3 GACCAAC 35 0.0012510786 40.000004 64 >>END_MODULE