FastQCFastQC Report
Wed 25 May 2016
SRR1780211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508892
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACAT250.00234277835.2267136
TACACAG508.412163E-526.3629155
AAACTCG450.001403758324.42450527
CTAGACG551.6071E-423.9662843
CTATGGC602.8854876E-421.98205630
GGTGTAA703.2456624E-521.9690931
GCGTCTA550.00446047120.01517535
TGTAGAT1900.019.69914637
TCTATGG708.1591244E-418.8417629
TAGATCT1658.185452E-1118.68083239
GGTATTG600.00746664518.3075791
TTTATAC600.00746664518.3075793
TAAGACC851.4442366E-418.0921944
GAAATAA750.001298258917.58218832
GTGAAAA750.001298258917.58218832
TACTGTC750.001299971417.57873243
GCCCTAC1002.5085545E-517.5752751
GTGTAGA2800.017.2988336
TAATACA902.2360808E-417.0870724
GTAGATC2007.094059E-1116.5125238
Produced by FastQC (version 0.11.5)