FastQCFastQC Report
Wed 25 May 2016
SRR1780211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508892
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT238924.69490579533575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC65221.2816078853666397No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG62931.2366081604741281No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG41940.8241434331842512TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC13950.274124961681457No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT8760.17213868561502244No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT6350.12478089653600372TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4750.036.59053444
ATGCCGT32600.035.3629944
GATATCT250.002329720235.266871
GTCTTCT4900.035.02142343
GTACAAG702.7284841E-1134.6371081
CGTCTTC5150.033.74855442
CCGTCTT5200.033.0009641
CTTTATA2200.031.9977842
GGAATCT353.216569E-431.42639734
AATATAG353.216569E-431.4263977
TCTTTAT2300.030.6668421
TATGCCG39000.029.55983443
ATTTACT300.00574111329.33418526
CGTTTAC300.00574387129.33130314
TTTATAC2750.028.7980083
GCCGTCT6250.028.1580540
CTAACTA554.9525224E-628.00081442
GGCTTAT406.9489656E-427.5522421
AAGGAAG757.000381E-826.3981725
TTAATAC858.218194E-925.8805622
Produced by FastQC (version 0.11.5)