FastQCFastQC Report
Wed 25 May 2016
SRR1780210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43164
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGTA150.002210381270.024
AGTTGGG200.006907006352.534
GCTAGGA200.006907006352.59
GGGTTGC200.006907006352.513
AACCTCT200.006907006352.534
AGAATTG200.006907006352.523
AATAAGA200.006907006352.524
CTCAGGC200.006907006352.53
GTAGATC400.002393807535.038
TAGATCT400.002393807535.039
CTGGGCC450.004263103531.11111353
GGCAACC1051.9823201E-523.33333470
AGGCAAC1301.02797225E-418.84615569
GAGGCAA1605.020561E-415.312568
CGAGGCA1900.001841657712.89473667
CTGCCGA4502.012365E-48.55555570
CGCTGCC4652.8691662E-48.2795768
ACGCTGC4753.6092894E-48.10526467
GCTGCCG4804.040015E-48.02083369
GACGCTG4955.622406E-47.77777766
Produced by FastQC (version 0.11.5)