##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43164 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.017514595496248 34.0 31.0 34.0 12.0 34.0 2 30.273607635992956 34.0 32.0 34.0 12.0 34.0 3 30.06405801130572 34.0 31.0 34.0 11.0 34.0 4 30.085765916041144 34.0 31.0 34.0 11.0 34.0 5 29.919076081920117 34.0 31.0 34.0 11.0 34.0 6 32.924080252061906 38.0 34.0 38.0 11.0 38.0 7 33.22338059494023 38.0 34.0 38.0 12.0 38.0 8 33.2106153275878 38.0 34.0 38.0 11.0 38.0 9 33.32035955889167 38.0 34.0 38.0 12.0 38.0 10-11 33.107404318413494 38.0 34.0 38.0 11.0 38.0 12-13 33.17266703734593 38.0 34.0 38.0 11.0 38.0 14-15 33.07923269391159 38.0 34.0 38.0 11.0 38.0 16-17 33.08878926883514 38.0 34.0 38.0 11.0 38.0 18-19 33.09963163747567 38.0 34.0 38.0 11.0 38.0 20-21 33.12223148920397 38.0 34.0 38.0 11.0 38.0 22-23 33.11817718469095 38.0 34.0 38.0 11.0 38.0 24-25 33.13522843109999 38.0 34.0 38.0 11.0 38.0 26-27 33.112199981466034 38.0 34.0 38.0 11.0 38.0 28-29 33.19436335835418 38.0 34.0 38.0 11.0 38.0 30-31 33.16807988138263 38.0 34.0 38.0 11.0 38.0 32-33 33.2576684273932 38.0 34.0 38.0 11.0 38.0 34-35 33.19582290797887 38.0 34.0 38.0 11.0 38.0 36-37 33.1381822815309 38.0 34.0 38.0 11.0 38.0 38-39 33.10512232415903 38.0 34.0 38.0 11.0 38.0 40-41 33.181806598091 38.0 34.0 38.0 11.0 38.0 42-43 33.23124594569549 38.0 34.0 38.0 11.0 38.0 44-45 33.30042396441479 38.0 34.0 38.0 11.0 38.0 46-47 33.19247521082384 38.0 34.0 38.0 11.0 38.0 48-49 33.1610138078028 38.0 34.0 38.0 11.0 38.0 50-51 33.087700398480216 38.0 34.0 38.0 11.0 38.0 52-53 33.16892549346677 38.0 34.0 38.0 11.0 38.0 54-55 33.1134973589102 38.0 34.0 38.0 11.0 38.0 56-57 33.07456445185803 38.0 34.0 38.0 11.0 38.0 58-59 33.1250579186359 38.0 34.0 38.0 11.0 38.0 60-61 33.15318320822908 38.0 34.0 38.0 11.0 38.0 62-63 33.04399499582986 38.0 34.0 38.0 11.0 38.0 64-65 32.97128394032063 38.0 34.0 38.0 11.0 38.0 66-67 32.93720461495691 38.0 32.5 38.0 11.0 38.0 68-69 32.86754007969604 38.0 33.0 38.0 11.0 38.0 70-71 32.78352330645909 38.0 33.0 38.0 11.0 38.0 72-73 32.888576128255025 38.0 33.0 38.0 11.0 38.0 74-75 32.92560930404967 38.0 33.0 38.0 11.0 38.0 76 31.27594754888333 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6642745917392432 1101 2 -0.2242156107966906 1101 3 0.07062533280734584 1101 4 0.05568162774613228 1101 5 -1.0341622258932937 1101 6 -0.4067487439325106 1101 7 -0.5317877189752167 1101 8 -1.1381772348894472 1101 9 -0.34186032843896896 1101 10-11 -0.3776286532786699 1101 12-13 -0.07984576768782148 1101 14-15 -0.03381215851806729 1101 16-17 0.34723608210941137 1101 18-19 -0.19589911149206785 1101 20-21 -0.5467115048505065 1101 22-23 -0.36614204907415626 1101 24-25 -0.31803589254010234 1101 26-27 -0.6785804857582747 1101 28-29 -1.154920838255638 1101 30-31 -0.501486054760214 1101 32-33 -1.5016101747744877 1101 34-35 -0.7782565984969949 1101 36-37 -0.39256734102124824 1101 38-39 -0.5980318844792762 1101 40-41 -0.43252831707564354 1101 42-43 -0.7466005721162006 1101 44-45 -0.49752076404825374 1101 46-47 -0.48387348712654443 1101 48-49 -0.9403532786897344 1101 50-51 -1.1646533210169565 1101 52-53 -0.669946294359427 1101 54-55 -0.4300045008067883 1101 56-57 -0.4172806876819948 1101 58-59 -0.036302382338192274 1101 60-61 -0.4746421693302523 1101 62-63 -0.6031886713818508 1101 64-65 -0.5033366661113021 1101 66-67 -0.6914651849699851 1101 68-69 -0.2794106302335848 1101 70-71 -0.4937083601697623 1101 72-73 -0.7959259312574929 1101 74-75 -0.9544237040218455 1101 76 -2.219249309727587 1102 1 0.2230559537464103 1102 2 -0.23986674580266154 1102 3 -0.3831978452093807 1102 4 0.7552634676505505 1102 5 -0.8737082235038081 1102 6 -0.06642616328735329 1102 7 -0.6370445887482106 1102 8 -0.6942106876970939 1102 9 -0.6372008302310803 1102 10-11 0.02114673501284159 1102 12-13 -1.3078624940916441 1102 14-15 -0.4402339028430333 1102 16-17 -1.0309897243422022 1102 18-19 -0.8536350732602855 1102 20-21 -0.7352718393785835 1102 22-23 -1.2571516070191961 1102 24-25 -0.5484719737826396 1102 26-27 -0.7797752288885036 1102 28-29 -0.5141203603583833 1102 30-31 -0.5424060069704879 1102 32-33 -0.09468066461916891 1102 34-35 -0.9317810907311639 1102 36-37 -0.6489293481897107 1102 38-39 -0.41335446512443497 1102 40-41 -0.27093930871244964 1102 42-43 -0.10571048848418485 1102 44-45 -0.660125304072146 1102 46-47 -1.6036166173535449 1102 48-49 -0.5487463491795808 1102 50-51 -0.16904400202054148 1102 52-53 -0.4474253863546451 1102 54-55 -0.45049434549017775 1102 56-57 -0.6908469959257246 1102 58-59 -0.33349473598215695 1102 60-61 -0.4897855385058705 1102 62-63 -0.41770480041410707 1102 64-65 0.06371829207268576 1102 66-67 -0.029219067885158267 1102 68-69 -0.013401072288537819 1102 70-71 0.13693680112055517 1102 72-73 -0.6286021558692028 1102 74-75 -0.08480004810787278 1102 76 0.2681461502485192 1103 1 -0.237843113259526 1103 2 -0.15120403729262577 1103 3 -0.0909727957324229 1103 4 1.0196683305286953 1103 5 -0.2807763603429372 1103 6 0.04960437106379345 1103 7 0.10980302222153426 1103 8 -0.9601422681324863 1103 9 -0.34678522378494137 1103 10-11 -0.2739903868418381 1103 12-13 -0.03472141408033025 1103 14-15 -1.0140817965382567 1103 16-17 -1.0607607167086144 1103 18-19 -0.175122209251235 1103 20-21 -0.7144349719768286 1103 22-23 -0.731169135998563 1103 24-25 -0.9837278403362149 1103 26-27 -0.33654650398039365 1103 28-29 -0.4185874228407158 1103 30-31 -0.4204099651271136 1103 32-33 -0.9228118492389044 1103 34-35 -1.3650578789697825 1103 36-37 -0.7223814262219364 1103 38-39 -0.7728964498572637 1103 40-41 -0.5552197723822232 1103 42-43 -1.0851563465559906 1103 44-45 -1.3657798135434476 1103 46-47 -0.76954819778895 1103 48-49 -0.4104653200588544 1103 50-51 -0.44928149141833273 1103 52-53 -0.6638517363687839 1103 54-55 -0.5763354537740355 1103 56-57 -0.7080649772658418 1103 58-59 -0.5545860563101215 1103 60-61 -0.03210954189857773 1103 62-63 -0.18106357904006387 1103 64-65 -0.7209862598074395 1103 66-67 -0.09616846002538892 1103 68-69 -0.8294598791870449 1103 70-71 -0.8997044202534852 1103 72-73 -0.5626705754337991 1103 74-75 -0.8036295646473093 1103 76 0.4258504194031687 1104 1 0.18428871935874014 1104 2 1.03891130231845 1104 3 0.44059236836185534 1104 4 0.47556625218691195 1104 5 0.7698170535981106 1104 6 0.6664205520411173 1104 7 -0.21309216917535423 1104 8 -0.19845507807286822 1104 9 -0.35269150338599076 1104 10-11 0.9414495195492094 1104 12-13 0.40278903334820626 1104 14-15 0.12062038209884207 1104 16-17 0.45587158952641005 1104 18-19 0.3420394333631265 1104 20-21 -0.3346500497057008 1104 22-23 0.21765239406217773 1104 24-25 0.20159561205137777 1104 26-27 0.6488270960641884 1104 28-29 0.7887452790036065 1104 30-31 0.5726250825131629 1104 32-33 0.4271630769326009 1104 34-35 0.6786541620398552 1104 36-37 0.8788889810884726 1104 38-39 0.30051414801425125 1104 40-41 -0.14049864907470777 1104 42-43 0.48482749475453346 1104 44-45 0.6790744796531882 1104 46-47 0.34250395577432613 1104 48-49 0.3431703850729164 1104 50-51 0.30435421371343807 1104 52-53 0.4461945109148857 1104 54-55 0.33047348365283113 1104 56-57 0.42504919223323867 1104 58-59 0.5276839609629675 1104 60-61 0.7476029448680066 1104 62-63 0.9472587032355264 1104 64-65 1.0647996654168352 1104 66-67 0.5903808239775046 1104 68-69 0.3913569704257043 1104 70-71 0.41722877192347596 1104 72-73 0.6427955191781081 1104 74-75 0.9139834068799715 1104 76 0.9044262259446505 1105 1 -0.5279194491373893 1105 2 0.43658222224935983 1105 3 0.22963789129047996 1105 4 0.14180573510884997 1105 5 0.3833457770616455 1105 6 0.14120742449890855 1105 7 0.14033737336657026 1105 8 -0.7311346345447021 1105 9 0.011993402169260037 1105 10-11 0.6878416742268669 1105 12-13 -0.5728893530116324 1105 14-15 0.1118724019350239 1105 16-17 0.3056514970318389 1105 18-19 0.2141907678480024 1105 20-21 -0.3365723765569868 1105 22-23 -0.35330654057871413 1105 24-25 -0.5066286036949919 1105 26-27 -0.35944726733917065 1105 28-29 -0.19339658314606112 1105 30-31 -0.2830053849744445 1105 32-33 -0.17472024618547266 1105 34-35 -0.28490520721405943 1105 36-37 -0.027724937672324756 1105 38-39 -0.17747660252901198 1105 40-41 0.1776046550986976 1105 42-43 0.3843093022989663 1105 44-45 0.4166629345481567 1105 46-47 0.1044976037377694 1105 48-49 0.05900032879610251 1105 50-51 -0.18210591889925354 1105 52-53 0.67584292011977 1105 54-55 0.4160309584396984 1105 56-57 -0.43325581696050364 1105 58-59 -0.18054025478340208 1105 60-61 -0.024475954112318732 1105 62-63 0.19679901637978503 1105 64-65 0.019471755459740336 1105 66-67 0.12520558577613627 1105 68-69 -0.27602476468322124 1105 70-71 -0.006574649261125387 1105 72-73 -0.49015149146433856 1105 74-75 -0.4257669692274604 1105 76 -0.6046839317418673 1106 1 0.31194484263529887 1106 2 0.5156888097528913 1106 3 0.34272808071654737 1106 4 0.6441523565394398 1106 5 3.6585057026528034E-4 1106 6 0.355796058934871 1106 7 0.04443689273326612 1106 8 0.13541894193254223 1106 9 1.4294658364355826 1106 10-11 -0.47514956128345176 1106 12-13 0.4365819503527959 1106 14-15 -0.5291227917319219 1106 16-17 -0.1606193539718319 1106 18-19 0.37969826007304874 1106 20-21 0.5758392717325194 1106 22-23 0.713218763351172 1106 24-25 0.8663428410321785 1106 26-27 0.005409956296681173 1106 28-29 0.18217593593791293 1106 30-31 0.15018658562210874 1106 32-33 0.2386526687141597 1106 34-35 0.28303372408365846 1106 36-37 -0.07485527411563453 1106 38-39 0.16442331265334076 1106 40-41 0.488319950546817 1106 42-43 -0.1094141921878844 1106 44-45 -0.05642160036844501 1106 46-47 0.08156856783163846 1106 48-49 -0.2117093121425455 1106 50-51 0.21243747946093805 1106 52-53 0.522944984015723 1106 54-55 0.09024639525055989 1106 56-57 0.3869307818520511 1106 58-59 0.014653412165991142 1106 60-61 0.7546589059385695 1106 62-63 -0.006593689302995642 1106 64-65 0.6526071809615743 1106 66-67 -0.15514499381108493 1106 68-69 -0.30228996117742923 1106 70-71 -0.4001002359164829 1106 72-73 0.3010274737604277 1106 74-75 -0.38480004810786994 1106 76 0.6162942983966673 1107 1 -0.3372615065710498 1107 2 -0.11182441776033514 1107 3 -0.00912377113530738 1107 4 0.2944169068039919 1107 5 0.056979607184022285 1107 6 0.19706590020471282 1107 7 -0.1836054353090617 1107 8 -0.5444752379616418 1107 9 -0.19593098896124417 1107 10-11 0.27617319003930163 1107 12-13 0.12970364347449248 1107 14-15 -0.6923503049594331 1107 16-17 0.24758170422922632 1107 18-19 0.5937194240942176 1107 20-21 0.16763821353146113 1107 22-23 0.8621605622929707 1107 24-25 -0.3156677409783981 1107 26-27 -0.12686517597845182 1107 28-29 -0.3432208894589124 1107 30-31 -0.8532525678170444 1107 32-33 -0.6817177016562113 1107 34-35 -0.42252183147190436 1107 36-37 -0.009246808507164417 1107 38-39 -0.6244655762355507 1107 40-41 -0.11353190130503776 1107 42-43 -0.27687450964820215 1107 44-45 0.29331384936701 1107 46-47 -0.6395954533323831 1107 48-49 -0.07149767193090639 1107 50-51 -0.11031384329038474 1107 52-53 -0.24239893132818935 1107 54-55 0.04183369683786253 1107 56-57 0.0655022104234817 1107 58-59 -0.6324365349239542 1107 60-61 -0.10565855437889127 1107 62-63 -0.28675241946172747 1107 64-65 -0.5010965227421238 1107 66-67 -0.7612296498957463 1107 68-69 -0.4700148289023005 1107 70-71 0.07106378524754575 1107 72-73 -0.6886550659221129 1107 74-75 -0.26733973064754935 1107 76 -0.6466686645662953 1108 1 0.3135321442226022 1108 2 -0.08801489395080964 1108 3 -0.05674281875435483 1108 4 0.6912423036293873 1108 5 0.3982494484538641 1108 6 0.4748436779824914 1108 7 -0.0010657527693780366 1108 8 0.011080317593915368 1108 9 0.3199420269117752 1108 10-11 1.3634747773408904 1108 12-13 0.9947830085538598 1108 14-15 0.5497131871040537 1108 16-17 1.2991690058165304 1108 18-19 1.260386090760882 1108 20-21 0.33430488019813254 1108 22-23 0.5248589749913819 1108 24-25 0.4898878145771519 1108 26-27 0.7659919668786941 1108 28-29 0.5853505391125111 1108 30-31 0.7142077496432719 1108 32-33 0.5166949967564918 1108 34-35 0.5417638828138109 1108 36-37 0.6494833502229866 1108 38-39 0.601724899954931 1108 40-41 0.2753569875838551 1108 42-43 0.7469350141613234 1108 44-45 0.694107500160662 1108 46-47 1.5231029593660281 1108 48-49 0.9046928042595752 1108 50-51 0.31032107734453973 1108 52-53 0.047283608354348416 1108 54-55 0.6053257603299258 1108 56-57 0.9504228453441144 1108 58-59 0.9429602904728753 1108 60-61 1.0252938265734883 1108 62-63 0.10213646942715826 1108 64-65 0.32430030265469867 1108 66-67 0.6395640008979058 1108 68-69 0.3276042187167505 1108 70-71 0.32106378524754575 1108 72-73 0.1922973150302667 1108 74-75 0.5104380471302292 1108 76 0.32952181162417915 1109 1 -0.7066931501962408 1109 2 -0.2484091591837867 1109 3 -0.4616327317087823 1109 4 -0.5814151464474158 1109 5 -1.2115815807320018 1109 6 -0.011587453609934073 1109 7 -0.29791675123328076 1109 8 -0.6462417510184792 1109 9 -1.0515377477938088 1109 10-11 -0.44214478231092613 1109 12-13 -0.2814586709136293 1109 14-15 -0.5660702230341954 1109 16-17 -0.27776391789058863 1109 18-19 -0.24831846633077248 1109 20-21 -0.37735666614083385 1109 22-23 -0.15243237165480394 1109 24-25 -0.3704552473788141 1109 26-27 -0.2229353244679544 1109 28-29 -0.6710498705137056 1109 30-31 -0.4934215386311749 1109 32-33 0.07903498651582908 1109 34-35 -0.36696627591634723 1109 36-37 -0.6707931474728639 1109 38-39 -0.3762576909308848 1109 40-41 -0.7188186396562912 1109 42-43 -0.9966005721162006 1109 44-45 -0.3523594737256701 1109 46-47 -0.2500025193846085 1109 48-49 -0.9040629561090938 1109 50-51 -0.8098146113395401 1109 52-53 -0.6739785524239394 1109 54-55 -0.5630690169358203 1109 56-57 -0.42937746187553927 1109 58-59 -0.5363023823381852 1109 60-61 -0.5552873306205726 1109 62-63 -0.6031886713818508 1109 64-65 -0.6888205370790459 1109 66-67 -0.09065873335708119 1109 68-69 -0.47699127539487307 1109 70-71 -0.22757932791169821 1109 72-73 -0.8039904473865249 1109 74-75 -0.006843058860560802 1109 76 -0.7111847935985551 1110 1 -0.2859478776712123 1110 2 1.0288433435970816 1110 3 0.9257421292478369 1110 4 0.5960042083912924 1110 5 0.3629456717707775 1110 6 0.8490910780919592 1110 7 0.7312823588890822 1110 8 0.9116002957000973 1110 9 1.2999639207267748 1110 10-11 0.2515426481673799 1110 12-13 1.3459050665724632 1110 14-15 0.5860432363651356 1110 16-17 1.0560792411750342 1110 18-19 0.9983444925123877 1110 20-21 0.5219759256277996 1110 22-23 0.6936466049859078 1110 24-25 0.8311576558469937 1110 26-27 0.945863340715583 1110 28-29 0.4390724876620524 1110 30-31 0.28980982955570056 1110 32-33 0.48782252822884686 1110 34-35 0.439068206842272 1110 36-37 0.6574882763313639 1110 38-39 0.13750760384107963 1110 40-41 0.15993553164515362 1110 42-43 0.6866586047469809 1110 44-45 1.3724736742164865 1110 46-47 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2116 44-45 -2.3846175382417982 2116 46-47 -1.995970261320096 2116 48-49 -2.882557579765006 2116 50-51 -1.4643845038126564 2116 52-53 -1.6672581223164116 2116 54-55 -2.190757188978832 2116 56-57 -2.2438935909078026 2116 58-59 -2.2379152855639965 2116 60-61 -2.6560937822334765 2116 62-63 -2.2241564133173313 2116 64-65 -2.157906558584422 2116 66-67 -2.4226479806689127 2116 68-69 -2.6423138560400368 2116 70-71 -2.1200524461912664 2116 72-73 -2.174958189322009 2116 74-75 -2.6708215534842097 2116 76 -1.7918299548888754 2117 1 1.0792651040732082 2117 2 1.0799589622583383 2117 3 1.6817259020673099 2117 4 0.0018865613324692276 2117 5 0.05483208150708663 2117 6 0.8747503073008858 2117 7 0.7036961519925242 2117 8 0.2591662186209902 2117 9 0.7575704115989836 2117 10-11 0.8207110051653501 2117 12-13 0.521767135537992 2117 14-15 0.7429904980284263 2117 16-17 0.28665733448132613 2117 18-19 -0.008754898968348357 2117 20-21 -0.0662086585506998 2117 22-23 0.02868717293723222 2117 24-25 0.3576282440822922 2117 26-27 0.008895795076639956 2117 28-29 -0.17076524053268116 2117 30-31 -0.5245410832232054 2117 32-33 -0.17485495655817118 2117 34-35 -0.02873098179870226 2117 36-37 -0.034550263222385524 2117 38-39 -0.207472112183261 2117 40-41 -0.344577652939023 2117 42-43 0.12261195160296268 2117 44-45 -0.4161165894751946 2117 46-47 -0.16788676985899542 2117 48-49 -0.217155935236228 2117 50-51 0.06755730516900371 2117 52-53 -0.05505065868580061 2117 54-55 0.23991959786494022 2117 56-57 -0.19607575409565925 2117 58-59 -0.12804811288847162 2117 60-61 0.20956086672288166 2117 62-63 -0.4059400945317506 2117 64-65 -0.30338410444147 2117 66-67 0.09278528941331388 2117 68-69 0.8301719124609122 2117 70-71 -0.3336514341735608 2117 72-73 -0.5525672974814029 2117 74-75 -0.11813338144120422 2117 76 0.923919570727822 2118 1 0.8792651040732089 2118 2 0.43290013872892885 2118 3 1.1523141373614258 2118 4 0.8607100907442344 2118 5 0.13718502268355692 2118 6 -0.19583792799323163 2118 7 0.4566373284631169 2118 8 1.2356368068562844 2118 9 1.239923352775456 2118 10-11 0.45012276987123556 2118 12-13 0.7982377237732763 2118 14-15 -0.17465656079510694 2118 16-17 0.7043043933048594 2118 18-19 0.6030098069140024 2118 20-21 0.6279089885081248 2118 22-23 1.2522165847019338 2118 24-25 0.9105694205528749 2118 26-27 0.5853663833119285 2118 28-29 1.129234759467323 2118 30-31 0.7989883285415047 2118 32-33 1.3133803375594724 2118 34-35 0.5418572534954151 2118 36-37 0.8830967956011406 2118 38-39 0.38076318193438397 2118 40-41 0.9554223470609813 2118 42-43 1.399082539838254 2118 44-45 0.5485892928777432 2118 46-47 0.8909367595527726 2118 48-49 0.5593146529990705 2118 50-51 0.3087337757572328 2118 52-53 1.1508316942553805 2118 54-55 0.39286077433552435 2118 56-57 -0.2078404599780086 2118 58-59 0.13077541652328506 2118 60-61 0.09779616084053089 2118 62-63 0.8293540231153074 2118 64-65 0.8554394249702924 2118 66-67 0.6045499952956632 2118 68-69 0.7831130889315006 2118 70-71 1.3839956246499696 2118 72-73 0.26507976134212896 2118 74-75 0.8289254420882131 2118 76 0.4415666295513532 2119 1 0.8856822163726719 2119 2 0.4148711089351913 2119 3 0.9555226935111598 2119 4 1.4206795330666182 2119 5 1.1688122190166368 2119 6 1.3803270834658932 2119 7 0.2023974506938231 2119 8 0.232581039094633 2119 9 0.1633763703461213 2119 10-11 0.34760176146787103 2119 12-13 0.7711177848886379 2119 14-15 1.0172456546365254 2119 16-17 0.4261531328006498 2119 18-19 1.3877309681057568 2119 20-21 1.236541532434785 2119 22-23 0.30263675276916757 2119 24-25 1.094144668834005 2119 26-27 0.7353281862149146 2119 28-29 0.07957853334050924 2119 30-31 1.1911197265552502 2119 32-33 1.012005242066735 2119 34-35 0.6427739838239148 2119 36-37 1.220911921651556 2119 38-39 0.8567754050054361 2119 40-41 1.0376224998493697 2119 42-43 1.1838037010300084 2119 44-45 0.799085855139019 2119 46-47 0.6244738107368804 2119 48-49 0.8852122847790511 2119 50-51 0.50224026926373 2119 52-53 0.372680433751178 2119 54-55 0.7860616910658607 2119 56-57 1.166873061794341 2119 58-59 0.3552979528105311 2119 60-61 1.3330138842935497 2119 62-63 0.601775719066417 2119 64-65 0.6702598986142974 2119 66-67 0.14389300522691428 2119 68-69 0.7449923861049186 2119 70-71 0.23953420371795886 2119 72-73 0.7251255978585505 2119 74-75 0.9584899951821768 2119 76 1.7624222445246112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 106.0 14 402.0 15 792.0 16 1069.0 17 1165.0 18 1108.0 19 1007.0 20 734.0 21 493.0 22 408.0 23 283.0 24 294.0 25 255.0 26 291.0 27 302.0 28 379.0 29 445.0 30 592.0 31 712.0 32 941.0 33 1317.0 34 1825.0 35 2922.0 36 5742.0 37 19574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.53822629969419 18.81197294041331 14.115929941618017 29.533870818274487 2 21.434528773978315 18.5687146696321 31.762579927717542 28.234176628672042 3 19.75025484199796 22.29635807617459 26.334445371142618 31.61894171068483 4 16.272819942544714 17.903808729496802 27.244926327495133 38.578445000463354 5 18.8907422852377 30.337781484570474 28.926883514039474 21.84459271615235 6 31.32239829487536 31.232045222870912 15.705217310721897 21.740339171531833 7 29.332313965341488 26.605504587155966 17.906125474932814 26.156055972569735 8 25.55138541377073 31.234361968306924 17.143916226485032 26.07033639143731 9 23.779075155221943 17.319988879621906 18.22351959966639 40.67741636548976 10-11 27.856547122602173 22.1376610138078 22.259290149198407 27.746501714391623 12-13 25.93017329255861 19.00194606616625 22.523399128903716 32.54448151237142 14-15 25.536326568436664 20.093133166527664 20.652627189324438 33.717913075711245 16-17 26.86266333055324 21.948846260772868 20.861134278565473 30.327356130108424 18-19 26.22787508108609 21.070799740524514 21.69632100824761 31.005004170141788 20-21 25.637104994903158 21.536465573162822 22.10059308683162 30.725836345102397 22-23 27.498609952738395 20.70127884348068 21.095125567602633 30.704985636178293 24-25 26.490825688073393 22.190946158836066 20.530998053933832 30.787230099156705 26-27 25.63131313131313 22.50602353813363 21.71601334445371 30.146649986099526 28-29 26.26610138078028 22.089009359651563 22.599851728292094 29.045037531276062 30-31 26.512834769715504 21.053424149754424 22.651978500602354 29.78176257992772 32-33 25.26410897970531 22.078584005189512 22.72611435455472 29.931192660550458 34-35 25.617412658697063 21.860809934204433 22.662403855064404 29.859373552034103 36-37 25.865304420350295 20.81016587897322 23.264757668427393 30.0597720322491 38-39 24.81466036511908 20.884301732925586 25.098461681030486 29.202576220924847 40-41 24.8586785284033 20.489296636085626 24.84477805578723 29.807246779723844 42-43 23.565934575108887 20.898202205541654 24.606153275878047 30.929709943471412 44-45 22.990223334260033 20.508988972291725 26.13288851820962 30.367899175238627 46-47 23.28792512278751 21.353442683717912 25.997358910202944 29.361273283291634 48-49 23.63080344731721 21.477388564544526 25.060235381336298 29.831572606801966 50-51 23.14081178760078 22.504865165415623 24.1532295431378 30.2010935038458 52-53 22.488647947363543 22.153878231859885 24.701139838754518 30.656333982022055 54-55 22.698313409322584 21.802891298304143 24.34088592345473 31.157909368918542 56-57 22.920720971179687 22.115651932165694 24.0721434528774 30.891483643777224 58-59 23.73389861921972 22.083217496061533 23.30298396812158 30.879899916597164 60-61 23.13501992401075 21.146093967194883 24.06055972569734 31.658326383097023 62-63 22.72611435455472 22.02414048744324 23.923871744972665 31.325873413029377 64-65 24.02696691687517 21.777407098508018 22.657770364192384 31.537855620424427 66-67 23.155870632934853 22.954313780001854 21.71948846260773 32.17032712445557 68-69 23.24969882309332 23.223056250579184 21.81679177092021 31.710453155407286 70-71 24.43123899545918 23.264757668427393 21.895561115744602 30.408442220368826 72-73 23.862477990918357 22.784032990455007 21.38935223797609 31.96413678065054 74-75 22.91145398943564 23.96557316282087 21.718330089889722 31.404642757853768 76 22.868594198869427 22.66703734593643 21.45537948290242 33.008988972291725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 2.0 17 4.0 18 3.0 19 4.0 20 6.0 21 6.0 22 7.5 23 15.5 24 26.0 25 30.0 26 51.0 27 91.0 28 133.5 29 157.0 30 176.5 31 244.5 32 354.5 33 416.0 34 459.5 35 623.5 36 750.0 37 756.0 38 887.0 39 1135.0 40 1382.0 41 1573.0 42 1634.0 43 1697.5 44 1785.0 45 1856.5 46 1904.0 47 1908.5 48 1798.0 49 1590.5 50 1498.0 51 1417.5 52 1229.5 53 1035.0 54 948.0 55 940.0 56 847.0 57 761.0 58 760.0 59 804.5 60 860.5 61 961.0 62 1050.0 63 1055.5 64 1137.5 65 1237.5 66 1329.0 67 1397.0 68 1389.0 69 1421.5 70 1420.0 71 1378.0 72 1409.0 73 1357.0 74 1172.0 75 1070.0 76 942.0 77 715.0 78 503.5 79 391.0 80 309.5 81 177.0 82 88.5 83 51.0 84 35.0 85 14.5 86 7.5 87 5.0 88 3.5 89 3.0 90 3.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 2.0 98 3.0 99 4.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 43164.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.27754610323417 #Duplication Level Percentage of deduplicated Percentage of total 1 84.652679576347 67.95709387452507 2 10.54226429251681 16.926142155499953 3 2.813771608322992 6.776480400333611 4 0.9436957086375575 3.0303030303030303 5 0.46463305532307875 1.8649800759892505 6 0.24530316585379933 1.1815401723658605 7 0.12409454272603966 0.6973403762394588 8 0.10677902513635969 0.6857566490594014 9 0.046174713572479875 0.3336113427856547 >10 0.06060431156387983 0.5467519228987119 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.002316745436011491 0.0 5 0.0 0.0 0.0 0.009266981744045964 0.0 6 0.0 0.0 0.0 0.011583727180057455 0.0 7 0.0 0.0 0.0 0.011583727180057455 0.0 8 0.0 0.0 0.0 0.011583727180057455 0.0 9 0.0 0.0 0.0 0.013900472616068946 0.0 10 0.0 0.0 0.0 0.016217218052080436 0.0 11 0.0 0.0 0.0 0.016217218052080436 0.0 12 0.0 0.0 0.0 0.016217218052080436 0.0 13 0.0 0.0 0.0 0.016217218052080436 0.0 14 0.0 0.0 0.0 0.016217218052080436 0.0 15 0.0 0.0 0.0 0.016217218052080436 0.0 16 0.0 0.0 0.0 0.016217218052080436 0.0 17 0.0 0.0 0.0 0.01853396348809193 0.0 18 0.0 0.0 0.0 0.01853396348809193 0.0 19 0.0 0.0 0.0 0.027800945232137893 0.0 20 0.0 0.0 0.0 0.03243443610416087 0.0 21 0.0 0.0 0.0 0.041701417848206836 0.0 22 0.0 0.0 0.0 0.04633490872022982 0.0 23 0.0 0.0 0.0 0.048651654156241314 0.0 24 0.0 0.0 0.0 0.0509683995922528 0.0 25 0.0 0.0 0.0 0.05791863590028728 0.0 26 0.0 0.0 0.0 0.06718561764433324 0.0 27 0.0 0.0 0.0 0.13668798072467797 0.0 28 0.0 0.0 0.0 0.2270410527291261 0.0 29 0.0 0.0 0.0 0.29886016124548237 0.0 30 0.0 0.0 0.0 0.386896487813919 0.0 31 0.0 0.0 0.0 0.4841997961264016 0.0 32 0.0 0.0 0.0 0.6023538133629877 0.0 33 0.0 0.0 0.0 0.7668427393198035 0.0 34 0.0 0.0 0.0 1.0448521916411824 0.0 35 0.0 0.0 0.0 1.3182281530905384 0.0 36 0.0 0.0 0.0 1.769993513112779 0.0 37 0.0 0.0 0.0 2.3839310536558243 0.0 38 0.0 0.0 0.0 2.935316467426559 0.0 39 0.0 0.0 0.0 3.4310999907330184 0.0 40 0.0 0.0 0.0 3.943100732091558 0.0 41 0.0 0.0 0.0 4.473635436938189 0.0 42 0.0 0.0 0.0 5.004170141784821 0.0 43 0.0 0.0 0.0 5.594940227967751 0.0 44 0.0 0.0 0.0 6.1463256417384855 0.0 45 0.0 0.0 0.0 6.801964600129738 0.0 46 0.0 0.0 0.0 7.422852376980817 0.0 47 0.0 0.0 0.0 8.0043554814197 0.0 48 0.0 0.0 0.0 8.551107404318413 0.0 49 0.0 0.0 0.0 9.049207673060884 0.0 50 0.0 0.0 0.0 9.459271615234918 0.0 51 0.0 0.0 0.0 10.045408210545824 0.0 52 0.0 0.0 0.0 10.54814197016032 0.0 53 0.0 0.0 0.0 11.03234176628672 0.0 54 0.0 0.0 0.0 11.479473635436937 0.0 55 0.0 0.0 0.0 11.977573904179408 0.0 56 0.0 0.0 0.0 12.410805300713557 0.0 57 0.0 0.0 0.0 12.806968770271522 0.0 58 0.0 0.0 0.0 13.25178389398573 0.0 59 0.0 0.0 0.0 13.703549254007969 0.0 60 0.0 0.0 0.0 14.15531461403021 0.0 61 0.0 0.0 0.0 14.57464553794829 0.0 62 0.0 0.0 0.0 15.068112315818738 0.0 63 0.0 0.0 0.0 15.524511166713001 0.0 64 0.0 0.0 0.0 15.992493744787323 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTGTA 15 0.0022103812 70.0 24 AGTTGGG 20 0.0069070063 52.5 34 GCTAGGA 20 0.0069070063 52.5 9 GGGTTGC 20 0.0069070063 52.5 13 AACCTCT 20 0.0069070063 52.5 34 AGAATTG 20 0.0069070063 52.5 23 AATAAGA 20 0.0069070063 52.5 24 CTCAGGC 20 0.0069070063 52.5 3 GTAGATC 40 0.0023938075 35.0 38 TAGATCT 40 0.0023938075 35.0 39 CTGGGCC 45 0.0042631035 31.111113 53 GGCAACC 105 1.9823201E-5 23.333334 70 AGGCAAC 130 1.02797225E-4 18.846155 69 GAGGCAA 160 5.020561E-4 15.3125 68 CGAGGCA 190 0.0018416577 12.894736 67 CTGCCGA 450 2.012365E-4 8.555555 70 CGCTGCC 465 2.8691662E-4 8.27957 68 ACGCTGC 475 3.6092894E-4 8.105264 67 GCTGCCG 480 4.040015E-4 8.020833 69 GACGCTG 495 5.622406E-4 7.777777 66 >>END_MODULE