FastQCFastQC Report
Wed 25 May 2016
SRR1780210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43164
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT31047.191177833379668No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG10202.363080344731721RNA PCR Primer, Index 15 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG7971.8464461125011582No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6491.5035677879714577No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2290.5305347048466315No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT1530.35446205170975814RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT790.1830228894449078No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT600.13900472616068948No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG580.13437123528866646No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG500.11583727180057456No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC470.10888703549254007No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAA150.002210381270.042
CTTTATA207.842596E-570.02
AGACAGG200.006907006352.58
AGAGACA200.006907006352.56
TCTTTAT305.816375E-446.6666641
TTTATAC400.002393807535.03
ATCTCGT6100.030.40983837
TCAATCT6100.030.40983834
TCTCGTA6100.030.40983838
AATCTCG6150.030.16260136
GTCAATC6150.030.16260133
CAATCTC6150.030.16260135
CGTCAAT6150.030.16260132
CTCGTAT6400.030.07812539
TCGTATG6400.030.07812540
GTCTTCT6450.029.8449649
TTCTGCT6350.029.76377952
CGTATGC6500.029.61538341
CCGTCTT6500.029.61538347
TCTGCTT6400.029.5312553
Produced by FastQC (version 0.11.5)