##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43164 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.57297748123436 34.0 34.0 34.0 34.0 34.0 2 33.62413122046149 34.0 34.0 34.0 34.0 34.0 3 33.65401723658604 34.0 34.0 34.0 34.0 34.0 4 33.68754054304513 34.0 34.0 34.0 34.0 34.0 5 33.6694930960986 34.0 34.0 34.0 34.0 34.0 6 37.33238346770457 38.0 38.0 38.0 36.0 38.0 7 37.305022704105276 38.0 38.0 38.0 36.0 38.0 8 37.17709202112872 38.0 38.0 38.0 36.0 38.0 9 37.21093967194884 38.0 38.0 38.0 36.0 38.0 10-11 37.15778194791956 38.0 38.0 38.0 36.0 38.0 12-13 37.15549995366509 38.0 38.0 38.0 36.0 38.0 14-15 37.055810397553515 38.0 38.0 38.0 36.0 38.0 16-17 37.066224168288386 38.0 38.0 38.0 35.5 38.0 18-19 36.991984060791395 38.0 38.0 38.0 35.0 38.0 20-21 37.04034612176814 38.0 38.0 38.0 35.0 38.0 22-23 37.02923732740247 38.0 38.0 38.0 35.5 38.0 24-25 37.020364192382544 38.0 38.0 38.0 35.5 38.0 26-27 36.8745945695487 38.0 37.5 38.0 34.5 38.0 28-29 36.82883884718747 38.0 37.0 38.0 34.5 38.0 30-31 36.819757205078304 38.0 37.0 38.0 34.5 38.0 32-33 36.71528356964137 38.0 37.0 38.0 34.0 38.0 34-35 36.6939463441757 38.0 37.0 38.0 34.0 38.0 36-37 36.708576591604114 38.0 37.0 38.0 34.0 38.0 38-39 36.06408117876008 38.0 37.0 38.0 34.0 38.0 40-41 36.36520016680567 38.0 37.0 38.0 34.0 38.0 42-43 36.64897831526272 38.0 37.0 38.0 34.0 38.0 44-45 36.74584144194236 38.0 37.0 38.0 34.0 38.0 46-47 36.67516912241683 38.0 37.0 38.0 34.0 38.0 48-49 36.68021962746734 38.0 37.0 38.0 34.0 38.0 50-51 36.465885923454735 38.0 37.0 38.0 34.0 38.0 52-53 36.48180196460013 38.0 37.0 38.0 34.0 38.0 54-55 36.41284403669725 38.0 37.0 38.0 34.0 38.0 56-57 36.47135344268372 38.0 37.0 38.0 34.0 38.0 58-59 36.34914512093411 38.0 37.0 38.0 34.0 38.0 60-61 36.34104809563525 38.0 37.0 38.0 34.0 38.0 62-63 36.28962793068298 38.0 37.0 38.0 34.0 38.0 64-65 36.1663539060328 38.0 37.0 38.0 33.0 38.0 66-67 35.72149244740988 38.0 37.0 38.0 31.0 38.0 68-69 35.58554582522473 38.0 37.0 38.0 31.0 38.0 70-71 34.71968538596979 38.0 35.5 38.0 25.5 38.0 72-73 33.580680659809104 38.0 34.0 38.0 20.0 38.0 74-75 33.236956723195256 38.0 34.0 38.0 15.0 38.0 76 32.39294319340191 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2524830694544349 1101 2 -0.15523943031067233 1101 3 -0.10973537323365434 1101 4 0.1834479796584887 1101 5 -0.29263214640222657 1101 6 -0.14587201710484976 1101 7 0.17985690791031317 1101 8 -0.1570798135161624 1101 9 0.30470090488246626 1101 10-11 0.1054069001316833 1101 12-13 -0.057227731449629005 1101 14-15 0.2761742744497653 1101 16-17 0.13237399900855706 1101 18-19 0.06369031333276354 1101 20-21 0.10549629088809098 1101 22-23 -0.15959545183468293 1101 24-25 -0.03285122417558739 1101 26-27 -0.13033300962726457 1101 28-29 -0.10500843420693684 1101 30-31 0.06393437326291718 1101 32-33 0.12481892795877769 1101 34-35 0.16152929158374718 1101 36-37 0.1626572517688487 1101 38-39 0.22125637451224378 1101 40-41 -0.06852213506139293 1101 42-43 -0.3552514425492319 1101 44-45 0.04587581495309223 1101 46-47 -0.1806226064028067 1101 48-49 0.24234900491064337 1101 50-51 0.22558511793980784 1101 52-53 0.1729733991308251 1101 54-55 -0.16000859905675924 1101 56-57 -0.25452135715565305 1101 58-59 -0.35916994053354756 1101 60-61 -0.44733962263055815 1101 62-63 -0.5360072868289905 1101 64-65 -0.24416754010821506 1101 66-67 -0.2793406584695788 1101 68-69 -1.4822621991452252 1101 70-71 -0.5879537947161353 1101 72-73 -0.9703278114732683 1101 74-75 -0.869923968606571 1101 76 -0.933171842030788 1102 1 -0.6916109069693661 1102 2 -0.4061952248148586 1102 3 -0.11672462054548305 1102 4 -0.24851139907507758 1102 5 0.047511222773401585 1102 6 0.15012559340888743 1102 7 0.2131902412436446 1102 8 0.04375650667499542 1102 9 0.04848824060528756 1102 10-11 -0.04286072232948612 1102 12-13 0.24773045254080728 1102 14-15 0.06751238675562377 1102 16-17 0.23718284010772095 1102 18-19 0.09645614367924082 1102 20-21 -0.1901130281083212 1102 22-23 0.01725640001716755 1102 24-25 0.1657449526463921 1102 26-27 -0.02749549469297108 1102 28-29 0.08892824440715685 1102 30-31 -0.003658219329672363 1102 32-33 0.36412597694324944 1102 34-35 0.29318998453475587 1102 36-37 0.07218532823240764 1102 38-39 0.404261153484768 1102 40-41 0.4419617359063537 1102 42-43 0.12634951324527322 1102 44-45 -0.09728428062636141 1102 46-47 0.08242996229492405 1102 48-49 -0.04845147298660635 1102 50-51 0.12866158150014684 1102 52-53 0.19489693557049037 1102 54-55 -0.0655641546123178 1102 56-57 0.15521579935569463 1102 58-59 0.1903820307926125 1102 60-61 -0.02168251390892806 1102 62-63 0.011603226910551712 1102 64-65 -0.38048773126711666 1102 66-67 -0.5571483048256098 1102 68-69 0.01684174350710066 1102 70-71 -0.33807326902915946 1102 72-73 0.28926000214680414 1102 74-75 0.17248941251648375 1102 76 0.489588014600038 1103 1 -0.16919077691564866 1103 2 0.26380760243988277 1103 3 0.1972702903959913 1103 4 0.10003256473606115 1103 5 0.2524306460134369 1103 6 0.11139220353045687 1103 7 0.15313935065840667 1103 8 0.3347941091629849 1103 9 -0.22626437460534277 1103 10-11 0.10220571815680302 1103 12-13 0.27218620600419285 1103 14-15 0.23301987898067722 1103 16-17 0.21255745136019755 1103 18-19 0.1868434775780301 1103 20-21 0.206918354419237 1103 22-23 0.33181676190577747 1103 24-25 0.022092139527934762 1103 26-27 0.4790920088410928 1103 28-29 0.3670735653005721 1103 30-31 0.4484763579957374 1103 32-33 0.5024352786113297 1103 34-35 0.31136923248499926 1103 36-37 0.64600494938027 1103 38-39 0.4519569408752133 1103 40-41 0.5320380717842141 1103 42-43 0.4494764569829002 1103 44-45 0.3655938646945316 1103 46-47 0.2368546159562186 1103 48-49 0.6758629177399982 1103 50-51 -0.2501905530771822 1103 52-53 0.15542845946079353 1103 54-55 0.37243414903484506 1103 56-57 0.4624818440263141 1103 58-59 0.6240546346942253 1103 60-61 0.3193126797580135 1103 62-63 0.4743965545893758 1103 64-65 0.6567189410540593 1103 66-67 0.2608895803878468 1103 68-69 0.2704464932950543 1103 70-71 0.8649518935379916 1103 72-73 -0.19530318699654003 1103 74-75 -0.27167230645439844 1103 76 0.3045159196042775 1104 1 0.208253921362612 1104 2 0.21143116069474388 1104 3 0.21125591473432337 1104 4 0.1979876547232422 1104 5 0.2050132503484221 1104 6 0.39857111922667343 1104 7 0.39518537506359763 1104 8 0.08344290840519619 1104 9 0.16889916247605896 1104 10-11 0.37865860721904454 1104 12-13 0.048081898877654794 1104 14-15 0.05407632295459308 1104 16-17 0.1485913288884717 1104 18-19 0.06236584900501185 1104 20-21 0.2386785371795952 1104 22-23 0.355915497070427 1104 24-25 0.1486592538088729 1104 26-27 0.20840610033270934 1104 28-29 -0.022155832370351902 1104 30-31 0.280175249175322 1104 32-33 0.5279270042479283 1104 34-35 0.3155203440913965 1104 36-37 0.1372569692164518 1104 38-39 0.42707813104627235 1104 40-41 0.11787414466547119 1104 42-43 0.1269713029181574 1104 44-45 0.1120695988005096 1104 46-47 0.019548102332770156 1104 48-49 0.07423033290687187 1104 50-51 0.19300329547689188 1104 52-53 0.06778149896599928 1104 54-55 0.2724731527680646 1104 56-57 0.05575648602776795 1104 58-59 0.36077943549658187 1104 60-61 0.6090825577320516 1104 62-63 0.45639911769184494 1104 64-65 0.28420542904647306 1104 66-67 0.3692642391051777 1104 68-69 0.13646866970336902 1104 70-71 0.573984043758081 1104 72-73 0.8293248845474466 1104 74-75 0.9760849788857726 1104 76 1.180261169506771 1105 1 -0.14629001355687166 1105 2 0.05770073221087557 1105 3 0.09803364917461721 1105 4 0.24507073267498924 1105 5 0.0997588902882498 1105 6 0.36330060047702517 1105 7 -0.12166980964693863 1105 8 -0.03925169236373449 1105 9 0.048544785700002535 1105 10-11 0.022053046401076415 1105 12-13 0.18058315256908486 1105 14-15 0.030729802644792414 1105 16-17 0.28507653532966515 1105 18-19 0.0723396607841309 1105 20-21 0.1573000338085535 1105 22-23 0.30128241076074147 1105 24-25 0.44575626166533766 1105 26-27 0.4065729248716323 1105 28-29 0.6991346340028599 1105 30-31 0.437025976316356 1105 32-33 0.28106123280980455 1105 34-35 0.33808678973690576 1105 36-37 0.3330278501436226 1105 38-39 0.6771477805698751 1105 40-41 0.31066402598268184 1105 42-43 0.251003174540152 1105 44-45 0.0946014982823229 1105 46-47 0.2444882037424776 1105 48-49 0.24838200170945868 1105 50-51 0.2841605919609904 1105 52-53 0.16687884114018203 1105 54-55 -0.020695621957521837 1105 56-57 0.5960696302858608 1105 58-59 0.5858866957629232 1105 60-61 0.6590073362465603 1105 62-63 0.4629461729099873 1105 64-65 0.20633726166474275 1105 66-67 0.550965916265703 1105 68-69 0.2742632871881838 1105 70-71 0.3344175423929556 1105 72-73 0.758895286285906 1105 74-75 0.8962666248433138 1105 76 0.4877220264745077 1106 1 0.07135205599359296 1106 2 0.20121218259254903 1106 3 0.20920130538044646 1106 4 0.12185897129528911 1106 5 0.19565937092155394 1106 6 0.2241996674829636 1106 7 0.13170875976216223 1106 8 0.02153428445277683 1106 9 0.38182157393861615 1106 10-11 0.13491705544829102 1106 12-13 0.3699526747630273 1106 14-15 0.348993868237109 1106 16-17 0.3557013586262485 1106 18-19 0.36312281034591365 1106 20-21 0.44322030522500455 1106 22-23 0.2542934370542085 1106 24-25 0.4064856933871397 1106 26-27 0.2206526534551756 1106 28-29 0.3815208369997478 1106 30-31 0.2593047436332867 1106 32-33 0.32708893990620425 1106 34-35 0.4709677623125401 1106 36-37 0.3573705134175924 1106 38-39 0.36352041274403035 1106 40-41 0.4604802544248727 1106 42-43 0.13005321694897276 1106 44-45 0.31012312678104337 1106 46-47 0.2453929252578888 1106 48-49 0.23673371219857842 1106 50-51 0.16569861853717782 1106 52-53 0.4171191577927118 1106 54-55 0.17147288242472314 1106 56-57 0.26262320676310225 1106 58-59 0.20149314190372536 1106 60-61 0.6635026712762482 1106 62-63 0.4782698935772203 1106 64-65 0.6491418983625081 1106 66-67 0.45025910258178925 1106 68-69 1.0761010027663573 1106 70-71 0.4619267309708448 1106 72-73 0.5374081502949508 1106 74-75 0.6613783014053709 1106 76 1.4969954220074442 1107 1 0.250717135358677 1107 2 0.18692646830683657 1107 3 0.22295791913705898 1107 4 0.1620706115069268 1107 5 0.25703503229721036 1107 6 0.343247286530584 1107 7 0.3306505587039652 1107 8 0.15910042201891628 1107 9 0.5267951189121618 1107 10-11 0.06534033587157495 1107 12-13 0.06783627264663039 1107 14-15 0.42888804813128445 1107 16-17 0.2718389247638058 1107 18-19 -0.012538565315459493 1107 20-21 0.3249663369710376 1107 22-23 -0.014754181993417603 1107 24-25 0.11442220132364866 1107 26-27 0.09208122488374926 1107 28-29 0.34474835022726324 1107 30-31 0.4508391351676764 1107 32-33 0.33449634731361755 1107 34-35 0.1998037411485143 1107 36-37 0.7359419419890116 1107 38-39 0.2092876085112252 1107 40-41 0.20624745019205903 1107 42-43 0.4387833756791366 1107 44-45 0.3347263013842223 1107 46-47 -0.018628238763284344 1107 48-49 0.45445857992343974 1107 50-51 0.3694023222408802 1107 52-53 0.420029210702765 1107 54-55 0.11380092475275916 1107 56-57 -0.1101281159882177 1107 58-59 0.29514393555452045 1107 60-61 0.3484233061968922 1107 62-63 0.3848836501909787 1107 64-65 0.5121048613254757 1107 66-67 1.0211585734812658 1107 68-69 1.0922385689039231 1107 70-71 0.38388440292851556 1107 72-73 0.033704446591244164 1107 74-75 -0.34867460864753497 1107 76 0.6895880146000337 1108 1 -0.1778542932127536 1108 2 0.06787884925921617 1108 3 -0.007200811021668585 1108 4 -0.06015161071529462 1108 5 0.1221143973765777 1108 6 -0.33135588807259353 1108 7 0.0687457967991989 1108 8 0.17497343789192854 1108 9 0.026795118912161797 1108 10-11 0.11295938349061885 1108 12-13 -0.3845446797343186 1108 14-15 -0.01158814234490535 1108 16-17 0.24802940095428028 1108 18-19 0.39619159341469157 1108 20-21 -0.048049536044828756 1108 22-23 0.1400077227684946 1108 24-25 0.1937872806887242 1108 26-27 -0.1261727433702191 1108 28-29 0.34474835022726324 1108 30-31 -0.15233546800692466 1108 32-33 -0.2686782558609906 1108 34-35 -0.1573391159943469 1108 36-37 0.25975146579853714 1108 38-39 0.3957955450191619 1108 40-41 0.20227919622381307 1108 42-43 -0.4897880528923011 1108 44-45 0.10456757122549476 1108 46-47 0.20756223742719726 1108 48-49 0.31160143706630095 1108 50-51 -0.007581804743246323 1108 52-53 -0.24266920199565334 1108 54-55 -0.3703260593742215 1108 56-57 0.0089195030594027 1108 58-59 0.13244552285610212 1108 60-61 0.06270902048260041 1108 62-63 0.10313761844494707 1108 64-65 0.11924771846832982 1108 66-67 0.4656030179257087 1108 68-69 0.334302060967417 1108 70-71 0.7727732918174013 1108 72-73 0.775767938654738 1108 74-75 0.9965634865905599 1108 76 0.9911753161873378 1109 1 0.31203305957782135 1109 2 0.19959927936674404 1109 3 0.2047807557986019 1109 4 0.09473830223913637 1109 5 0.27188398263003677 1109 6 0.11219249902418227 1109 7 0.0508246498457936 1109 8 0.24614599293545325 1109 9 0.22405574359214597 1109 10-11 -0.09620600309412453 1109 12-13 0.26535291371165215 1109 14-15 0.24794846799815673 1109 16-17 0.4105998054601727 1109 18-19 0.5999806359134183 1109 20-21 0.21033500056551446 1109 22-23 0.3565335804233811 1109 24-25 0.3219874855018361 1109 26-27 0.5753121516630557 1109 28-29 0.4232173722446717 1109 30-31 0.4832892119725969 1109 32-33 0.29014150860393784 1109 34-35 -0.14089006325496456 1109 36-37 0.025560477575297114 1109 38-39 0.14867572935095552 1109 40-41 0.09680044558377432 1109 42-43 0.24958726712818446 1109 44-45 0.10232742785631643 1109 46-47 0.5734096516617058 1109 48-49 0.6899296500719316 1109 50-51 0.1852625372946477 1109 52-53 0.3745863023566329 1109 54-55 0.10208817513679236 1109 56-57 0.48338186865079535 1109 58-59 0.5480881239825806 1109 60-61 0.008305538659762135 1109 62-63 0.1051217454290736 1109 64-65 0.2598647179562974 1109 66-67 0.3254980512078376 1109 68-69 0.025802316983799756 1109 70-71 0.2910784633483843 1109 72-73 -1.240489101795852 1109 74-75 -0.5715368718323859 1109 76 0.12327977087243625 1110 1 0.3974056958403409 1110 2 0.00466045845462304 1110 3 0.030976528879804732 1110 4 -0.146084834579554 1110 5 0.19846907717953144 1110 6 -0.0037696811760383753 1110 7 -0.14772929898624199 1110 8 0.2881370941590333 1110 9 0.32249845771895025 1110 10-11 -0.09120044135885763 1110 12-13 0.3131838369597091 1110 14-15 0.06093511983352329 1110 16-17 0.14711259745127592 1110 18-19 -0.16114283460938594 1110 20-21 0.10452298721734365 1110 22-23 -0.4440782104553733 1110 24-25 0.19796078917258342 1110 26-27 0.2955568680145575 1110 28-29 0.19087588169962544 1110 30-31 0.046692993952575534 1110 32-33 -0.13102089406569206 1110 34-35 -0.496145903076993 1110 36-37 0.1502823908122224 1110 38-39 0.20860342679255695 1110 40-41 -0.029589988231585096 1110 42-43 8.386575620065173E-4 1110 44-45 -0.27378491919596826 1110 46-47 -0.3542877899400736 1110 48-49 0.17852809278605974 1110 50-51 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8 0.16012448755910924 2116 9 0.24824929197924206 2116 10-11 0.1054069001316833 2116 12-13 -0.3287331077937239 2116 14-15 -0.22651389759324303 2116 16-17 -0.7103679364753077 2116 18-19 -0.2951806544091653 2116 20-21 -0.25606284889685327 2116 22-23 -0.10448792495296289 2116 24-25 0.15666490485667595 2116 26-27 -0.17468784833694428 2116 28-29 0.49310984536295166 2116 30-31 7.623302521650999E-4 2116 32-33 -0.08485849139606216 2116 34-35 -0.2739545793839966 2116 36-37 -0.11288038263975864 2116 38-39 -1.2800877115092604 2116 40-41 -1.069866221082897 2116 42-43 -0.6778320877105202 2116 44-45 -0.3318123570899232 2116 46-47 0.04921610327460968 2116 48-49 -0.2200165864872119 2116 50-51 -0.049952516468792396 2116 52-53 0.2616830765501774 2116 54-55 0.22977634717980067 2116 56-57 0.09762918047875502 2116 58-59 -0.4680409082754835 2116 60-61 -0.3088987624155024 2116 62-63 -0.33977072768920635 2116 64-65 -0.46459764763509526 2116 66-67 -0.5226202283620509 2116 68-69 -0.7483912314032963 2116 70-71 -0.7680613215978553 2116 72-73 -2.8910267362044557 2116 74-75 -1.8860530008646386 2116 76 -2.4358600140738 2117 1 -0.256752519169801 2117 2 -0.1870231115250931 2117 3 -0.4618226597611681 2117 4 0.05432084493363476 2117 5 -0.08712930010241138 2117 6 -0.4254735351314167 2117 7 0.17397455496913494 2117 8 -0.6847837983358929 2117 9 -0.4974812583053918 2117 10-11 -0.11584547178482296 2117 12-13 -0.0076072380909977255 2117 14-15 0.026553781091131157 2117 16-17 -0.0695709725284388 2117 18-19 -0.2534349238588831 2117 20-21 -0.4325966882390446 2117 22-23 -0.08165427536409453 2117 24-25 -0.5163901236063282 2117 26-27 -0.1388244707278261 2117 28-29 -1.970331014852107 2117 30-31 -0.2345950385017872 2117 32-33 -0.2713860056275621 2117 34-35 -0.27129803289443544 2117 36-37 -0.5851131467131268 2117 38-39 -1.2445405894346209 2117 40-41 -1.4698029699760014 2117 42-43 -0.3104696588680227 2117 44-45 -0.7212494222385644 2117 46-47 -0.6190950921713068 2117 48-49 -1.491806592812317 2117 50-51 -0.7286368934453904 2117 52-53 -0.7301575306604491 2117 54-55 -0.14530271670381723 2117 56-57 -0.7195118694896223 2117 58-59 -0.4756310411028153 2117 60-61 -1.337804518266232 2117 62-63 -0.9875253133944568 2117 64-65 -0.6284180144915226 2117 66-67 -0.18024198674282133 2117 68-69 -2.0093020473425725 2117 70-71 -0.5650885195738198 2117 72-73 -0.6721779063499298 2117 74-75 -0.3547437029519287 2117 76 -0.5993008742888506 2118 1 -0.5273407544639213 2118 2 -0.681140758583922 2118 3 -0.09711677740822466 2118 4 0.12490908022775216 2118 5 -0.18124694716123457 2118 6 -0.22547353513141388 2118 7 -0.17896662150145204 2118 8 -0.4730190924535407 2118 9 -0.05042243477597452 2118 10-11 -0.35113958943188806 2118 12-13 -0.3546660616204136 2118 14-15 -0.7263873953794544 2118 16-17 -0.6225121489990286 2118 18-19 -0.6475525709177035 2118 20-21 -0.1914202176508084 2118 22-23 -0.49341898124644246 2118 24-25 -1.304625417723969 2118 26-27 -0.5094127060219478 2118 28-29 -0.5879780736756288 2118 30-31 -0.3051832737959046 2118 32-33 -0.23020953503932162 2118 34-35 -0.6007097976003166 2118 36-37 -0.1439366761248948 2118 38-39 -0.6974817659052093 2118 40-41 -0.7050970876230593 2118 42-43 -0.5692931882797794 2118 44-45 -0.15066118694443986 2118 46-47 -0.6249774451124921 2118 48-49 -0.8271007104593835 2118 50-51 -1.01098983462186 2118 52-53 -1.5419222365428027 2118 54-55 -1.0982438931744056 2118 56-57 -1.537158928313147 2118 58-59 -0.6873957469851604 2118 60-61 -0.9789809888544738 2118 62-63 -1.0875253133944511 2118 64-65 -0.6872415439032906 2118 66-67 -1.40377139850753 2118 68-69 -0.7387138120484593 2118 70-71 -1.7003826372208763 2118 72-73 -0.5545308475264079 2118 74-75 -1.1370966441283947 2118 76 -1.2345949919359107 2119 1 -0.1482727636312262 2119 2 0.14147192285228982 2119 3 0.2979939941731331 2119 4 0.20680365623997687 2119 5 -0.4168466415844634 2119 6 -0.058628615345320156 2119 7 0.25128547933888257 2119 8 0.4635737264922213 2119 9 -0.008558416441367456 2119 10-11 0.026018546549785526 2119 12-13 0.32180452661488346 2119 14-15 -0.8561047368615036 2119 16-17 -0.24367334074845814 2119 18-19 0.04373849096159432 2119 20-21 -0.01594275393804878 2119 22-23 -0.060569477808705585 2119 24-25 -0.37475528785384427 2119 26-27 -0.16044402764150334 2119 28-29 0.04785080332971603 2119 30-31 -0.2591175747890304 2119 32-33 -1.3668023539850793 2119 34-35 0.07606636741114414 2119 36-37 0.331540787587862 2119 38-39 -0.5327758835522687 2119 40-41 -0.4268694329248177 2119 42-43 -0.0031358162400607625 2119 44-45 0.4187811354390547 2119 46-47 0.4698277496927119 2119 48-49 0.27083664630151105 2119 50-51 0.328276781115342 2119 52-53 -0.1488741082005589 2119 54-55 -0.39052807957623514 2119 56-57 -0.6545004103605123 2119 58-59 -0.6990840586734777 2119 60-61 -0.009080301306724436 2119 62-63 -0.023125007817682786 2119 64-65 -0.6834218342012193 2119 66-67 -1.0589280066053206 2119 68-69 -0.03222030555494371 2119 70-71 -1.638121369077254 2119 72-73 0.2999382128250119 2119 74-75 0.4428116828387587 2119 76 -1.0954047703927472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 4.0 23 7.0 24 15.0 25 40.0 26 69.0 27 131.0 28 206.0 29 372.0 30 568.0 31 864.0 32 1237.0 33 2078.0 34 3329.0 35 4998.0 36 7002.0 37 22243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.40765452692058 15.360022240756185 12.051709758131777 36.18061347419145 2 20.0606987304235 18.20730238161431 35.506440552312114 26.225558335650078 3 19.690019460661663 16.64118246687054 31.822815309053844 31.845982763413954 4 16.21953479751645 23.27865814104346 28.8712816235752 31.630525437864886 5 17.243536280233528 28.180891483643776 37.33435270132518 17.241219534797516 6 31.093040496710223 34.841534612176815 14.933741080530073 19.131683810582892 7 28.783245297006765 22.724955981836718 20.308590492076732 28.18320822907979 8 24.344361041608746 35.128811046242234 14.704383282364933 25.82244462978408 9 22.368177184690946 14.54221110184413 15.464275785376703 47.62533592808822 10-11 28.056945602817162 24.98957464553795 21.80057455286813 25.15290519877676 12-13 26.20818274487999 16.928458900935965 24.141645815957745 32.7217125382263 14-15 23.504540821054583 16.19405059772032 18.352098971365027 41.949309609860066 16-17 29.91150032434436 23.355110740431844 17.127699008432952 29.605689926790845 18-19 32.19001946066166 21.27467333889352 17.47521082383468 29.06009637661014 20-21 26.556852932999718 19.505838198498747 17.404550088036327 36.5327587804652 22-23 24.88879621907145 23.258965804837363 18.50963766101381 33.342600315077384 24-25 30.087572977481237 23.037716615698265 19.80817347789825 27.066536928922254 26-27 31.659484755815033 24.53201742192568 18.027754610323417 25.780743211935874 28-29 25.596561949772962 20.356083773514968 23.878695208970438 30.168659067741636 30-31 24.337410805300713 19.651793160967472 30.006486887220834 26.00430914651098 32-33 27.452275044018165 18.303447317208786 23.242748586785282 31.00152905198777 34-35 26.676165322954315 17.955935501807062 24.851728292095267 30.516170883143364 36-37 25.53053470484663 26.570753405615793 20.75224724307293 27.146464646464647 38-39 23.438513576128255 22.80835881753313 25.492308405152443 28.260819201186173 40-41 24.666388657214345 17.73352793994996 25.805069039013993 31.795014363821707 42-43 29.82693911592994 17.16360856269113 24.925864146047633 28.083588175331293 44-45 23.568251320544896 22.60680196460013 28.16351589287369 25.66143082198128 46-47 27.96775090353072 18.954452784728016 24.952506718561764 28.125289593179502 48-49 27.561162079510705 17.37095727921416 21.353442683717912 33.71443795755722 50-51 25.2212491891391 17.918867574830877 25.470299323510332 31.38958391251969 52-53 21.74728940783987 17.784496339542212 33.51288110462423 26.9553331479937 54-55 22.50602353813363 19.26373830043555 27.82527105921601 30.40496710221481 56-57 26.561486423871745 20.573857844500047 22.530349365211748 30.33430636641646 58-59 22.84426837179131 21.107867667500695 28.577054953201742 27.470809007506254 60-61 26.672690204800297 25.477249559818365 22.31489203966268 25.535168195718654 62-63 22.09480122324159 33.19896209804467 19.303122972847746 25.403113705865998 64-65 21.31405801130572 35.13576128255027 18.396117134649245 25.154063571494767 66-67 21.462329719210455 34.152302844963394 18.389166898341212 25.996200537484942 68-69 20.78815679733111 34.51255676026318 19.04596422945047 25.653322212955242 70-71 21.15883606709295 32.61861736632379 19.819757205078307 26.402789361504958 72-73 21.04415716801038 29.281345565749234 21.007089241034198 28.667408025206193 74-75 21.140302103604856 28.235335001390048 21.671995181169493 28.952367713835603 76 21.37661013807803 27.780094523213787 21.571216754702995 29.27207858400519 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 2.5 17 5.0 18 2.5 19 2.0 20 2.0 21 0.0 22 5.5 23 13.0 24 16.5 25 18.0 26 33.0 27 55.5 28 80.0 29 97.0 30 116.5 31 172.0 32 261.5 33 315.0 34 334.0 35 406.0 36 529.5 37 600.0 38 740.0 39 1585.0 40 2768.0 41 3143.5 42 3041.0 43 2705.5 44 2095.5 45 1715.0 46 1609.0 47 1567.0 48 1416.0 49 1232.5 50 1158.0 51 1080.0 52 911.5 53 773.0 54 725.0 55 707.5 56 694.0 57 665.0 58 632.0 59 675.0 60 764.5 61 850.0 62 889.0 63 918.0 64 1004.0 65 1150.0 66 1233.5 67 1228.0 68 1300.0 69 1424.0 70 1487.0 71 1498.0 72 1418.5 73 1305.0 74 1174.5 75 1078.0 76 927.0 77 664.5 78 464.5 79 376.0 80 303.0 81 162.5 82 66.0 83 37.0 84 28.5 85 11.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 43164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.172180520804375 #Duplication Level Percentage of deduplicated Percentage of total 1 78.02951259502161 48.51264943008062 2 14.655686391414518 18.22351959966639 3 3.8120435236249812 7.110091743119266 4 1.6693993143538528 4.1516078213325915 5 0.849605008197943 2.6410897970531 6 0.39871813981219256 1.4873505699193772 7 0.18259055000745267 0.7946436845519415 8 0.14160083469965717 0.7042906125474934 9 0.0521687285735579 0.2919099249374479 >10 0.1751378744969444 1.8371791307571124 >50 0.011179013265762408 0.4563988508942638 >100 0.007452675510508272 0.8849967565563897 >500 0.007452675510508272 3.350013900472616 >1k 0.007452675510508272 9.55425817811139 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 3104 7.191177833379668 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 1020 2.363080344731721 RNA PCR Primer, Index 15 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 797 1.8464461125011582 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 649 1.5035677879714577 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 229 0.5305347048466315 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 153 0.35446205170975814 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 79 0.1830228894449078 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT 60 0.13900472616068948 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 58 0.13437123528866646 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 50 0.11583727180057456 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 47 0.10888703549254007 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.002316745436011491 0.0 5 0.0 0.0 0.0 0.009266981744045964 0.0 6 0.0 0.0 0.0 0.011583727180057455 0.0 7 0.0 0.0 0.0 0.011583727180057455 0.0 8 0.0 0.0 0.0 0.011583727180057455 0.0 9 0.0 0.0 0.0 0.013900472616068946 0.0 10 0.0 0.0 0.0 0.016217218052080436 0.0 11 0.0 0.0 0.0 0.016217218052080436 0.0 12 0.0 0.0 0.0 0.016217218052080436 0.0 13 0.0 0.0 0.0 0.016217218052080436 0.0 14 0.0 0.0 0.0 0.016217218052080436 0.0 15 0.0 0.0 0.0 0.016217218052080436 0.0 16 0.0 0.0 0.0 0.01853396348809193 0.0 17 0.0 0.0 0.0 0.020850708924103418 0.0 18 0.0 0.0 0.0 0.020850708924103418 0.0 19 0.0 0.0 0.0 0.03706792697618386 0.0 20 0.0 0.0 0.0 0.041701417848206836 0.0 21 0.0 0.0 0.0 0.041701417848206836 0.0 22 0.0 0.0 0.0 0.04633490872022982 0.0 23 0.0 0.0 0.0 0.048651654156241314 0.0 24 0.0 0.0 0.0 0.048651654156241314 0.0 25 0.0 0.0 0.0 0.055601890464275786 0.0 26 0.0 0.0 0.0 0.06486887220832174 0.0 27 0.0 0.0 0.0 0.132054489852655 0.0 28 0.0 0.0 0.0 0.22009081642109166 0.0 29 0.0 0.0 0.0 0.29422667037345934 0.0 30 0.0 0.002316745436011491 0.0 0.3799462515058845 0.0 31 0.0 0.002316745436011491 0.0 0.4633490872022982 0.0 32 0.0 0.002316745436011491 0.0 0.5815031044388843 0.0 33 0.0 0.002316745436011491 0.0 0.7344083032156427 0.0 34 0.0 0.002316745436011491 0.0 1.005467519228987 0.0 35 0.0 0.002316745436011491 0.0 1.2765267352423315 0.0 36 0.0 0.002316745436011491 0.0 1.7259753498285608 0.0 37 0.0 0.002316745436011491 0.0 2.316745436011491 0.0 38 0.0 0.002316745436011491 0.0 2.85886386803818 0.0 39 0.0 0.002316745436011491 0.0 3.3430636641645814 0.0 40 0.0 0.002316745436011491 0.0 3.85274766008711 0.0 41 0.0 0.002316745436011491 0.0 4.3601149105736265 0.0 42 0.0 0.004633490872022982 0.0 4.8790658882402 0.0 43 0.0 0.004633490872022982 0.0 5.444351774627004 0.0 44 0.0 0.004633490872022982 0.0 5.988786952089704 0.002316745436011491 45 0.0 0.004633490872022982 0.0 6.614308219812807 0.002316745436011491 46 0.0 0.004633490872022982 0.0 7.22592901491984 0.002316745436011491 47 0.0 0.004633490872022982 0.0 7.800481883050691 0.002316745436011491 48 0.0 0.004633490872022982 0.0 8.32869984246131 0.002316745436011491 49 0.0 0.004633490872022982 0.0 8.805949402279678 0.002316745436011491 50 0.0 0.004633490872022982 0.0 9.220646835325734 0.002316745436011491 51 0.0 0.004633490872022982 0.0 9.799833194328608 0.002316745436011491 52 0.0 0.004633490872022982 0.0 10.297933463071077 0.002316745436011491 53 0.0 0.004633490872022982 0.0 10.784450004633491 0.002316745436011491 54 0.0 0.004633490872022982 0.0 11.222314892039662 0.002316745436011491 55 0.0 0.004633490872022982 0.0 11.708831433602075 0.002316745436011491 56 0.0 0.004633490872022982 0.0 12.142062830136224 0.002316745436011491 57 0.0 0.004633490872022982 0.0 12.535909554258177 0.002316745436011491 58 0.0 0.004633490872022982 0.0 12.985358168844407 0.002316745436011491 59 0.0 0.004633490872022982 0.0 13.425539801686591 0.002316745436011491 60 0.0 0.004633490872022982 0.0 13.856454452784728 0.002316745436011491 61 0.0 0.004633490872022982 0.0 14.257251413214716 0.002316745436011491 62 0.0 0.004633490872022982 0.0 14.736817718469094 0.002316745436011491 63 0.0 0.004633490872022982 0.0 15.188583078491336 0.002316745436011491 64 0.0 0.004633490872022982 0.0 15.656565656565656 0.002316745436011491 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGAA 15 0.0022103812 70.0 42 CTTTATA 20 7.842596E-5 70.0 2 AGACAGG 20 0.0069070063 52.5 8 AGAGACA 20 0.0069070063 52.5 6 TCTTTAT 30 5.816375E-4 46.666664 1 TTTATAC 40 0.0023938075 35.0 3 ATCTCGT 610 0.0 30.409838 37 TCAATCT 610 0.0 30.409838 34 TCTCGTA 610 0.0 30.409838 38 AATCTCG 615 0.0 30.162601 36 GTCAATC 615 0.0 30.162601 33 CAATCTC 615 0.0 30.162601 35 CGTCAAT 615 0.0 30.162601 32 CTCGTAT 640 0.0 30.078125 39 TCGTATG 640 0.0 30.078125 40 GTCTTCT 645 0.0 29.84496 49 TTCTGCT 635 0.0 29.763779 52 CGTATGC 650 0.0 29.615383 41 CCGTCTT 650 0.0 29.615383 47 TCTGCTT 640 0.0 29.53125 53 >>END_MODULE