FastQCFastQC Report
Wed 25 May 2016
SRR1780209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496721
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG353.2387624E-431.3893876
GACGTTA300.00575704229.31741725
CGTTACT300.00575704229.31741727
CCGGAAT450.001392453624.45828413
ACGAGAG602.866317E-422.0035824
ACAAAGT602.889853E-421.97699541
TTGTGTG500.00259706321.9725697
TACAAAG550.00445228120.02141840
CAATGTG708.171396E-418.83742532
CAGCAAA600.00732629718.36779420
AATTGCA600.00733056618.36593816
GCAAACC600.007420691718.32707822
GCATTAG600.00745093518.31416331
GTATTAT600.00745959418.3104741
TACCTCA600.00745959418.3104745
CTAGCCT600.00745959418.3104744
CTAAGCC800.00200276116.4794253
ACATCCC800.00200276116.4794258
TAGATCT1401.5639853E-615.73111439
TCAAAGT850.002953479315.54761114
Produced by FastQC (version 0.11.5)