FastQCFastQC Report
Wed 25 May 2016
SRR1780209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496721
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT220974.448573746630402No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC58831.1843670793060894No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG57471.1569875241835959No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG42940.8644691889410756RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC13990.28164704129682455No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT5970.12018819417741548TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT2050.036.5755121
TCTTCTG5150.033.74695644
CTTTATA2200.033.002722
ATGCCGT30750.032.55225444
GTCTTCT5600.031.03514543
CGTCTTC5900.030.20283542
GATCCTA300.00567481229.4038411
ATTCGCA300.00574231129.33279839
CAGTATT300.00574231129.33279814
AATATCG752.1609594E-929.33279436
TTATCAA300.00574513729.32984232
TTCTAGC407.0228754E-427.5022662
TATTACA407.0228754E-427.5022664
ATCGTAT804.303729E-927.49949839
ATATCGT804.303729E-927.49949837
TATGCCG37500.026.69284443
TCTAGCA508.32705E-526.4021763
TTTATAC2800.025.9307063
TATCGTA858.212737E-925.8818838
CCGTCTT7100.024.47842641