Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780209_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 496721 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 61 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT | 22097 | 4.448573746630402 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC | 5883 | 1.1843670793060894 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG | 5747 | 1.1569875241835959 | No Hit |
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG | 4294 | 0.8644691889410756 | RNA PCR Primer, Index 13 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC | 1399 | 0.28164704129682455 | No Hit |
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT | 597 | 0.12018819417741548 | TruSeq Adapter, Index 8 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTTTAT | 205 | 0.0 | 36.575512 | 1 |
TCTTCTG | 515 | 0.0 | 33.746956 | 44 |
CTTTATA | 220 | 0.0 | 33.00272 | 2 |
ATGCCGT | 3075 | 0.0 | 32.552254 | 44 |
GTCTTCT | 560 | 0.0 | 31.035145 | 43 |
CGTCTTC | 590 | 0.0 | 30.202835 | 42 |
GATCCTA | 30 | 0.005674812 | 29.403841 | 1 |
ATTCGCA | 30 | 0.005742311 | 29.332798 | 39 |
CAGTATT | 30 | 0.005742311 | 29.332798 | 14 |
AATATCG | 75 | 2.1609594E-9 | 29.332794 | 36 |
TTATCAA | 30 | 0.005745137 | 29.329842 | 32 |
TTCTAGC | 40 | 7.0228754E-4 | 27.502266 | 2 |
TATTACA | 40 | 7.0228754E-4 | 27.502266 | 4 |
ATCGTAT | 80 | 4.303729E-9 | 27.499498 | 39 |
ATATCGT | 80 | 4.303729E-9 | 27.499498 | 37 |
TATGCCG | 3750 | 0.0 | 26.692844 | 43 |
TCTAGCA | 50 | 8.32705E-5 | 26.402176 | 3 |
TTTATAC | 280 | 0.0 | 25.930706 | 3 |
TATCGTA | 85 | 8.212737E-9 | 25.88188 | 38 |
CCGTCTT | 710 | 0.0 | 24.478426 | 41 |