FastQCFastQC Report
Wed 25 May 2016
SRR1780208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90624
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGAG150.00221827770.0000142
ATCGATA150.00221827770.0000160
GTTTGTC200.00693148952.535
TAGAGGA200.00693148952.541
CGGAAGA200.00693148952.562
TGTAATC200.00693148952.556
TAAGAGA400.002407729435.04
CTGTAAT450.004287745331.11111355
GAGACAG450.004287745331.1111137
CGGACCG450.004287745331.1111135
TTAGGGA500.007175911727.99999868
GCGGATC500.007175911727.99999831
AGAGACA500.007175911727.9999986
AGGCAAC1905.4387783E-1022.10526570
ACCTGTG900.005240173519.44444570
GGCAACC1351.3943425E-418.14814870
GAGGCAA2259.120413E-817.1111169
CGAGGCA2758.89484E-714.068
ACGAGGC4001.4188117E-1013.99999970
GACGAGG5004.836693E-911.269
Produced by FastQC (version 0.11.5)