##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 90624 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.623267567090394 34.0 32.0 34.0 21.0 34.0 2 30.67685160663842 34.0 32.0 34.0 21.0 34.0 3 30.61282883121469 34.0 32.0 34.0 21.0 34.0 4 30.484904661016948 34.0 32.0 34.0 11.0 34.0 5 30.490013682909606 34.0 32.0 34.0 11.0 34.0 6 33.34035134180791 37.0 34.0 38.0 21.0 38.0 7 33.6192509710452 38.0 34.0 38.0 21.0 38.0 8 33.5819209039548 38.0 34.0 38.0 21.0 38.0 9 33.990013682909606 38.0 34.0 38.0 21.0 38.0 10-11 33.73239980579096 38.0 34.0 38.0 21.0 38.0 12-13 33.83352643891243 38.0 34.0 38.0 21.0 38.0 14-15 33.82104630120057 38.0 34.0 38.0 21.0 38.0 16-17 33.837692002118644 38.0 34.0 38.0 21.0 38.0 18-19 33.86449505649718 38.0 34.0 38.0 21.0 38.0 20-21 33.88285112111582 38.0 34.0 38.0 21.0 38.0 22-23 33.87136409781074 38.0 34.0 38.0 21.0 38.0 24-25 33.88829118114407 38.0 34.5 38.0 21.0 38.0 26-27 33.885902189265536 38.0 34.0 38.0 21.0 38.0 28-29 33.98786193502825 38.0 35.0 38.0 21.0 38.0 30-31 34.01477533545197 38.0 35.0 38.0 21.0 38.0 32-33 34.057523393361585 38.0 35.0 38.0 21.0 38.0 34-35 33.990063338629945 38.0 35.0 38.0 21.0 38.0 36-37 33.93758827683616 38.0 35.0 38.0 21.0 38.0 38-39 33.93102268714689 38.0 35.0 38.0 21.0 38.0 40-41 33.98705089159604 38.0 35.0 38.0 21.0 38.0 42-43 34.04919226694915 38.0 35.0 38.0 21.0 38.0 44-45 34.11355159781073 38.0 35.0 38.0 21.0 38.0 46-47 34.031117584745765 38.0 35.0 38.0 21.0 38.0 48-49 33.997594456214685 38.0 35.0 38.0 21.0 38.0 50-51 33.906967249293785 38.0 35.0 38.0 21.0 38.0 52-53 33.954449152542374 38.0 35.0 38.0 21.0 38.0 54-55 33.919243246822035 38.0 35.0 38.0 21.0 38.0 56-57 33.880561440677965 38.0 35.0 38.0 21.0 38.0 58-59 33.91971773481639 38.0 35.0 38.0 21.0 38.0 60-61 33.93129855225989 38.0 35.0 38.0 21.0 38.0 62-63 33.78572453213277 38.0 34.0 38.0 20.5 38.0 64-65 33.769409869350284 38.0 34.0 38.0 20.0 38.0 66-67 33.70942024187853 38.0 34.0 38.0 20.0 38.0 68-69 33.630081435381356 38.0 34.0 38.0 20.0 38.0 70-71 33.55003641419492 38.0 34.0 38.0 19.0 38.0 72-73 33.630980755649716 38.0 34.0 38.0 19.0 38.0 74-75 33.69371799964689 38.0 34.0 38.0 20.0 38.0 76 31.820047669491526 37.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.9506210214046433 1101 2 -0.4635062291309282 1101 3 -0.34494925921688946 1101 4 -0.8060253149880019 1101 5 -0.8742373682319737 1101 6 -0.8333995572467927 1101 7 -1.3680773723144029 1101 8 -0.9123458423992119 1101 9 -1.0037007913292086 1101 10-11 -1.4303625379816367 1101 12-13 -1.4184827213222775 1101 14-15 -1.3802673860418437 1101 16-17 -0.7758425067655139 1101 18-19 -0.9835459118318539 1101 20-21 -0.7909768028205093 1101 22-23 -0.8507800902566274 1101 24-25 -1.0968103892088124 1101 26-27 -1.4092084877469517 1101 28-29 -1.197786520387048 1101 30-31 -1.1990356383733953 1101 32-33 -1.3100482081886895 1101 34-35 -0.7784864233265409 1101 36-37 -0.5232950049632663 1101 38-39 -1.1511880287505463 1101 40-41 -0.9871798775134479 1101 42-43 -1.1803907559167897 1101 44-45 -0.8054222058070906 1101 46-47 -0.7857894482514425 1101 48-49 -1.321107904477337 1101 50-51 -0.8154638131050049 1101 52-53 -0.7137826833725995 1101 54-55 -0.7309633592797056 1101 56-57 -1.211668493990345 1101 58-59 -0.8670880637153431 1101 60-61 -0.8015519527943127 1101 62-63 -1.328403072162338 1101 64-65 -1.221904535069612 1101 66-67 -1.3594357956468386 1101 68-69 -0.8745380650598449 1101 70-71 -1.0642309445649403 1101 72-73 -0.8858081600105265 1101 74-75 -1.0461260024308316 1101 76 -1.6177187944448796 1102 1 0.25356692021173544 1102 2 0.2712273827108369 1102 3 0.8156056946229171 1102 4 0.472508905446265 1102 5 0.020793319271248834 1102 6 0.7102850061654493 1102 7 -2.7862044478865755E-4 1102 8 0.13817249273927246 1102 9 0.45551577476496163 1102 10-11 0.18784237201887777 1102 12-13 0.652659421129627 1102 14-15 0.6185979499734202 1102 16-17 0.8862145874631722 1102 18-19 0.5927666880959421 1102 20-21 0.48503021146593994 1102 22-23 0.2858824503591819 1102 24-25 0.6317792750338 1102 26-27 0.6358299361016293 1102 28-29 0.3921098125852538 1102 30-31 0.873879416554658 1102 32-33 0.5227113461978092 1102 34-35 0.6277103892991747 1102 36-37 0.7981501443481918 1102 38-39 0.7450160044641692 1102 40-41 0.5281574271438245 1102 42-43 0.7076643268621083 1102 44-45 0.9570397571616027 1102 46-47 0.5563181901001997 1102 48-49 0.509833608751812 1102 50-51 0.4281537019808752 1102 52-53 0.6465053665526099 1102 54-55 0.7220125548984342 1102 56-57 0.7920642926412569 1102 58-59 0.6410737806293909 1102 60-61 0.8523239562262575 1102 62-63 0.8966987897181085 1102 64-65 0.909703593250569 1102 66-67 0.5846175335604471 1102 68-69 0.7522026126440124 1102 70-71 0.5397777717174961 1102 72-73 0.6666829431923205 1102 74-75 0.5123801092819988 1102 76 -0.4142142300011251 1103 1 -0.16097298827834905 1103 2 -0.09647724362368137 1103 3 -0.2717608534197886 1103 4 0.05474073056065265 1103 5 -0.1270841591222407 1103 6 -1.1247556649072479 1103 7 -0.5442160886705096 1103 8 -0.3625011219023193 1103 9 -0.37787263397931525 1103 10-11 -0.11760373880979813 1103 12-13 0.27613425590339347 1103 14-15 -0.3529640734124442 1103 16-17 -0.27894809682762656 1103 18-19 -0.4501349387469702 1103 20-21 0.25454597150660874 1103 22-23 -0.2542738790765071 1103 24-25 -0.387571258774031 1103 26-27 -0.8262633531713774 1103 28-29 -0.6098983216292879 1103 30-31 -0.3547809799883055 1103 32-33 -0.6393183945240963 1103 34-35 -0.7895112680470362 1103 36-37 -0.4437918993732026 1103 38-39 -1.0452356477981581 1103 40-41 -0.5252233557743224 1103 42-43 -0.44490938945715897 1103 44-45 -0.42827417268080126 1103 46-47 -0.4539830300319778 1103 48-49 -0.4186234324276512 1103 50-51 -0.9634451795646228 1103 52-53 -1.0138085632897855 1103 54-55 -0.45407412532525626 1103 56-57 0.10704268613388024 1103 58-59 -0.49410669725570955 1103 60-61 -0.22336872298065202 1103 62-63 -0.6723212916240371 1103 64-65 -0.40588486633255627 1103 66-67 -0.8268529799118483 1103 68-69 -0.6642119781033244 1103 70-71 -0.3617982323496136 1103 72-73 -0.25187958858195714 1103 74-75 -0.8116539527413877 1103 76 -0.7486194155628922 1104 1 -0.1519612115246396 1104 2 -0.3270327991792392 1104 3 -0.2522268032405748 1104 4 0.26121794510238416 1104 5 -0.02154140438593899 1104 6 -0.023129960862185328 1104 7 -0.09205275021685111 1104 8 -0.5656885258961708 1104 9 0.037245133557803456 1104 10-11 -0.1779275995368792 1104 12-13 -0.1174269320126271 1104 14-15 -0.19708209696594992 1104 16-17 -0.29475710860438653 1104 18-19 -0.2872509141693911 1104 20-21 -0.611999087377157 1104 22-23 -0.1853990710887956 1104 24-25 0.0015416444517768468 1104 26-27 0.23879681067910496 1104 28-29 -0.1585158331500196 1104 30-31 -0.27683679156024255 1104 32-33 -0.5032264846418641 1104 34-35 0.02325114874763301 1104 36-37 -0.4813751046983512 1104 38-39 -0.10817727606237071 1104 40-41 -0.028807585165004923 1104 42-43 -0.1129137417357029 1104 44-45 0.15676807002273563 1104 46-47 -0.1668670546095541 1104 48-49 -0.5526608108198658 1104 50-51 0.27118103651320524 1104 52-53 -0.00502080087299106 1104 54-55 -0.1995234340810086 1104 56-57 0.19847253764437767 1104 58-59 0.1493136816485361 1104 60-61 0.17530639222671596 1104 62-63 0.19723195978405528 1104 64-65 0.1418557890693961 1104 66-67 0.14761578608917603 1104 68-69 -0.295081153730564 1104 70-71 0.285718408715411 1104 72-73 0.16168287941599147 1104 74-75 0.014318909521797707 1104 76 0.4450441789071533 1105 1 0.18970480108556842 1105 2 0.25407020163179084 1105 3 0.022764694025468657 1105 4 0.4439482383812994 1105 5 0.9958303351426174 1105 6 0.8793371400979666 1105 7 0.6197307309332416 1105 8 0.1333539354490938 1105 9 0.8991607341124492 1105 10-11 0.4372059587918713 1105 12-13 -0.34912643231349705 1105 14-15 0.52822727173875 1105 16-17 0.32702166333920957 1105 18-19 0.6709156347671126 1105 20-21 0.4713473270853399 1105 22-23 0.5022824295783437 1105 24-25 0.5572280112989603 1105 26-27 0.7737757500611551 1105 28-29 0.2938034510505929 1105 30-31 0.9133889887291105 1105 32-33 0.6428635658512931 1105 34-35 0.5008693367496306 1105 36-37 0.6714844301367009 1105 38-39 0.4078144043394829 1105 40-41 0.4948496369264106 1105 42-43 0.3662611006366845 1105 44-45 1.021947412303561 1105 46-47 0.697959097183869 1105 48-49 0.49605331418339915 1105 50-51 0.4718259361809416 1105 52-53 0.5234568173150151 1105 54-55 0.4751881270209424 1105 56-57 0.71672725339144 1105 58-59 0.6795637928381311 1105 60-61 0.6740113604395788 1105 62-63 0.5653872589494782 1105 64-65 0.04454526922532409 1105 66-67 0.7379671452184908 1105 68-69 0.6694559051083573 1105 70-71 0.42813920247833437 1105 72-73 0.6671115480186671 1105 74-75 0.36053061451617197 1105 76 0.6900406470022773 1106 1 -0.5556934851727604 1106 2 -0.33669463492802976 1106 3 0.36881885672513803 1106 4 0.7048442502293888 1106 5 -0.3416286725797981 1106 6 0.7934120369561057 1106 7 -0.4043092563723789 1106 8 0.0876541576007881 1106 9 -7.76029234117459E-5 1106 10-11 0.1149273170908316 1106 12-13 0.057966554040042695 1106 14-15 0.22480507772626623 1106 16-17 0.244085029466369 1106 18-19 -0.11035750603474526 1106 20-21 0.2289507334113665 1106 22-23 0.056828605395544685 1106 24-25 0.39956642238539075 1106 26-27 0.03463209196319639 1106 28-29 0.2714163781636785 1106 30-31 0.4078484195976202 1106 32-33 0.47980686427507635 1106 34-35 0.12368748971693577 1106 36-37 0.26656006750049954 1106 38-39 0.4919279132784382 1106 40-41 0.37151577466045893 1106 42-43 0.03337736002523428 1106 44-45 0.41921547535233117 1106 46-47 0.6145728705891429 1106 48-49 0.31113847233425673 1106 50-51 0.474391259358768 1106 52-53 0.38766659198972064 1106 54-55 0.3288192494159432 1106 56-57 0.2937662886183574 1106 58-59 0.31588295077741435 1106 60-61 -0.05698673540301513 1106 62-63 0.0810172176927324 1106 64-65 0.4465737257999507 1106 66-67 -0.09675463622654945 1106 68-69 0.4551720798676939 1106 70-71 0.3506241278988327 1106 72-73 0.3170903907141138 1106 74-75 0.270902983076418 1106 76 0.9619913504826556 1107 1 0.1435818771460795 1107 2 0.10895753898501326 1107 3 0.49925363933383693 1107 4 -0.8205180686111895 1107 5 -0.008295339246465971 1107 6 -0.38050100652215235 1107 7 -0.09996143028541837 1107 8 0.4789585054268741 1107 9 -0.05804861741616918 1107 10-11 -0.28000021914105844 1107 12-13 0.24093756853279302 1107 14-15 -0.0759195599548903 1107 16-17 0.35096908743738453 1107 18-19 0.21572945048698955 1107 20-21 0.20358841457078825 1107 22-23 -0.039185887358073046 1107 24-25 -0.04427415732475026 1107 26-27 0.07811035283276624 1107 28-29 0.5558366680187419 1107 30-31 0.1469788543802295 1107 32-33 0.03777787876782668 1107 34-35 -0.09008062622508817 1107 36-37 0.1288789080802104 1107 38-39 0.06076849298859344 1107 40-41 -0.023411761571423995 1107 42-43 -0.2238690167863524 1107 44-45 0.12392562027986287 1107 46-47 0.10913808798044045 1107 48-49 -0.1598760204193681 1107 50-51 -0.1741594652789118 1107 52-53 0.38766659198972064 1107 54-55 -0.00632567812028384 1107 56-57 0.0890561436908186 1107 58-59 -0.08810255646896081 1107 60-61 -0.11133456148996856 1107 62-63 -0.19072191274205608 1107 64-65 0.01903749391589571 1107 66-67 -0.01704449129901775 1107 68-69 0.17618657262131876 1107 70-71 0.17489949021766904 1107 72-73 0.034481695061941764 1107 74-75 -0.10228542272067642 1107 76 0.17938265483048 1108 1 -0.10167379690368605 1108 2 -0.19411875305764426 1108 3 0.3235221654481357 1108 4 0.31929598662534175 1108 5 0.7283740619020165 1108 6 0.8612955480307605 1108 7 0.3248539921920184 1108 8 0.16829402634566293 1108 9 -0.12025807872325345 1108 10-11 0.24294208329263256 1108 12-13 0.1966868165502973 1108 14-15 0.5276626112236684 1108 16-17 0.2581812832262784 1108 18-19 0.3882410720347025 1108 20-21 0.49231551328558965 1108 22-23 0.18625846593697304 1108 24-25 0.3386079865089897 1108 26-27 0.1747811212221535 1108 28-29 0.2732553171847343 1108 30-31 0.6421866749982215 1108 32-33 0.44764252191795606 1108 34-35 0.10637821982358275 1108 36-37 -0.05430402875325768 1108 38-39 0.45510127942555556 1108 40-41 0.4653426819615234 1108 42-43 0.3943426321061807 1108 44-45 0.2147514337882086 1108 46-47 0.3255654874882339 1108 48-49 0.2155409880575263 1108 50-51 0.606029213966373 1108 52-53 0.6570472318584635 1108 54-55 0.43194339489037503 1108 56-57 0.24905751093172057 1108 58-59 0.2972679658170634 1108 60-61 0.3500477190678595 1108 62-63 0.5223215655188156 1108 64-65 0.25937793690194866 1108 66-67 0.47938017372696606 1108 68-69 0.6030050030287555 1108 70-71 0.6414362689980919 1108 72-73 -0.06033646189732167 1108 74-75 0.5211627588489094 1108 76 0.18150187823764696 1109 1 -0.09350965313440796 1109 2 -0.49771298506854933 1109 3 -0.014536518819028288 1109 4 0.29550661866494465 1109 5 -0.20849371914396997 1109 6 0.4760455300954689 1109 7 -0.07041109138642554 1109 8 0.2317275581628664 1109 9 0.1236618581445299 1109 10-11 0.2279625295226353 1109 12-13 0.2470060647029655 1109 14-15 -0.08548036890402244 1109 16-17 0.03670915466612712 1109 18-19 0.4227126984067553 1109 20-21 0.24685314152290516 1109 22-23 -0.017873824758197543 1109 24-25 -0.073645403260727 1109 26-27 0.059973746231122504 1109 28-29 -0.1539776263549797 1109 30-31 -0.05177989362219648 1109 32-33 0.10966992704027234 1109 34-35 0.07905201277860385 1109 36-37 0.13127599850837868 1109 38-39 0.5991288280301958 1109 40-41 0.2561454655184505 1109 42-43 -0.2237863584957296 1109 44-45 -0.05620057137716117 1109 46-47 0.05853054954434356 1109 48-49 -0.5659210967405244 1109 50-51 0.053026846508160475 1109 52-53 -0.035936482898691224 1109 54-55 0.3342678084064161 1109 56-57 0.22168825919200685 1109 58-59 0.13989656184259758 1109 60-61 0.40368858283140696 1109 62-63 0.2470363944161562 1109 64-65 0.1604038354599524 1109 66-67 0.3743080738632756 1109 68-69 -0.4844115978751802 1109 70-71 0.4297281120284424 1109 72-73 -0.12820359727288633 1109 74-75 0.1639707077692023 1109 76 -0.3625113221354077 1110 1 -0.5578344601398229 1110 2 -0.16541663756307656 1110 3 0.04717854051960657 1110 4 0.47790090372082616 1110 5 -0.4901793972174815 1110 6 -0.08059982075140226 1110 7 0.0900912706368473 1110 8 0.08057247117127275 1110 9 -0.07072977683645831 1110 10-11 0.22979885859280103 1110 12-13 0.22932689659604222 1110 14-15 0.19573722528885895 1110 16-17 -0.3587641141436393 1110 18-19 -0.05576264437466705 1110 20-21 -0.2142310847704465 1110 22-23 -0.2790574288600567 1110 24-25 0.07076207455929762 1110 26-27 -0.036761188669217404 1110 28-29 -0.14409745582841538 1110 30-31 -0.2716640968714188 1110 32-33 -0.0256279183336261 1110 34-35 0.19788050684474 1110 36-37 -0.2080776136589222 1110 38-39 -0.3367940893566015 1110 40-41 0.24750391695295093 1110 42-43 0.4510321689843977 1110 44-45 -0.47702958393621486 1110 46-47 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0.35777969235206797 2116 24-25 0.3650329078926404 2116 26-27 0.021950932542907253 2116 28-29 0.2226165593231002 2116 30-31 0.37535294858312795 2116 32-33 0.0030179149997167087 2116 34-35 0.16852444623867768 2116 36-37 0.4091098863410778 2116 38-39 0.31495780458278944 2116 40-41 0.12581831089234896 2116 42-43 -0.11075760374287569 2116 44-45 0.2511901130334877 2116 46-47 0.1151389937775491 2116 48-49 -0.10031986099907897 2116 50-51 0.060215534721088204 2116 52-53 -0.426531777575498 2116 54-55 0.04190801753188822 2116 56-57 -0.31334421862802486 2116 58-59 -0.3026632448747577 2116 60-61 -0.3521633658377965 2116 62-63 -0.36101176781451016 2116 64-65 -0.18386105680873754 2116 66-67 0.08802797246909932 2116 68-69 -0.011086978103321599 2116 70-71 0.0071005771741994295 2116 72-73 -0.07070399334385513 2116 74-75 -0.18516585750328574 2116 76 0.12027939396091725 2117 1 0.08561086265332563 2117 2 0.18504449550675162 2117 3 0.39780436397151675 2117 4 0.7193370038525764 2117 5 -0.49199099142037994 2117 6 0.22819464565176162 2117 7 -0.051048386807160284 2117 8 0.3104802445573114 2117 9 0.7988354405548463 2117 10-11 0.6964490562212546 2117 12-13 0.19111872795308216 2117 14-15 0.11248623714656247 2117 16-17 -0.26497294154812323 2117 18-19 0.37967872584930973 2117 20-21 -0.12068694774805522 2117 22-23 0.2579155619172866 2117 24-25 0.5906896107911876 2117 26-27 0.30999441080378176 2117 28-29 -0.06191695516965723 2117 30-31 0.13792088336573727 2117 32-33 0.2687561396373894 2117 34-35 -0.44424729289175957 2117 36-37 0.022900647210640557 2117 38-39 -0.0035431012143121166 2117 40-41 -0.2725059644699783 2117 42-43 0.13210924408321034 2117 44-45 -0.1441903217491216 2117 46-47 0.16348591406740098 2117 48-49 -0.23686877404255569 2117 50-51 0.15464488254717423 2117 52-53 0.07516659198972064 2117 54-55 -0.0036082868159397208 2117 56-57 0.16333150600965496 2117 58-59 -0.36437067241099186 2117 60-61 -0.1520954310551872 2117 62-63 -0.2178958257855257 2117 64-65 -0.27715815825801826 2117 66-67 -0.1818995637627836 2117 68-69 -0.015842412885930912 2117 70-71 -0.05426717644899526 2117 72-73 -0.9510255513148707 2117 74-75 -0.5461260024308245 2117 76 -0.07967531618401225 2118 1 0.8891584753500439 2118 2 0.4371117134315341 2118 3 0.7629017232673299 2118 4 0.4844076893687159 2118 5 0.3099228522712707 2118 6 0.484863090570407 2118 7 0.9157094152733976 2118 8 -0.1701479229540226 2118 9 0.018994149543907213 2118 10-11 -0.3209889272409967 2118 12-13 0.3251044574809612 2118 14-15 0.39829578635968943 2118 16-17 0.331152691954145 2118 18-19 0.3812958237420787 2118 20-21 0.5788996086670011 2118 22-23 0.1186117475667885 2118 24-25 0.18183058171676691 2118 26-27 -0.017926368070590115 2118 28-29 0.2028536503251388 2118 30-31 -0.02145495018987731 2118 32-33 0.05361543544961478 2118 34-35 0.1767683112693561 2118 36-37 0.14008189554353123 2118 38-39 0.08603078515538698 2118 40-41 -0.006818447798863758 2118 42-43 0.3485502950301296 2118 44-45 0.3326701743831819 2118 46-47 0.0664266982765227 2118 48-49 0.54222231691503 2118 50-51 0.085042988708814 2118 52-53 0.13563271628952833 2118 54-55 -0.022796737069334938 2118 56-57 -0.01538281781002837 2118 58-59 0.0928345823235972 2118 60-61 0.12296125072661823 2118 62-63 0.2652663507948958 2118 64-65 -0.18565042286191158 2118 66-67 0.09390731807237529 2118 68-69 -0.016742652949943704 2118 70-71 0.5148012417958725 2118 72-73 0.5850507356949919 2118 74-75 -0.09550583705338767 2118 76 0.27445245122063255 2119 1 0.8503823230769605 2119 2 1.1772407050942668 2119 3 0.9256750440161099 2119 4 0.8386235144434373 2119 5 1.4136667566286718 2119 6 0.36782675267517106 2119 7 0.9047765853221605 2119 8 0.8267845923833974 2119 9 1.6568343257276439 2119 10-11 1.0076251989191434 2119 12-13 1.054064658833802 2119 14-15 0.8707080877597164 2119 16-17 0.8849713170917823 2119 18-19 0.8584273323153013 2119 20-21 0.7129250923857313 2119 22-23 0.8245264872238636 2119 24-25 0.7486466899439179 2119 26-27 1.2078483684403452 2119 28-29 0.6851566234256552 2119 30-31 0.6135340383267192 2119 32-33 0.7147566970509942 2119 34-35 0.728620600084831 2119 36-37 0.7085890530077421 2119 38-39 0.34240171483920534 2119 40-41 0.542084336533371 2119 42-43 0.5693305372827666 2119 44-45 0.6398119079052833 2119 46-47 0.3188649553160019 2119 48-49 0.58998462618041 2119 50-51 0.0886610475415992 2119 52-53 0.6497903378091152 2119 54-55 0.8532060944549684 2119 56-57 0.687857704448902 2119 58-59 0.9659665628175489 2119 60-61 0.4116587495468167 2119 62-63 0.6101420783393294 2119 64-65 0.6943440713963867 2119 66-67 0.7386689981101284 2119 68-69 0.293800842409496 2119 70-71 0.5099050643536813 2119 72-73 0.7712431220407581 2119 74-75 1.1425585014710649 2119 76 0.6230838811404027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 14.0 13 152.0 14 675.0 15 1114.0 16 1495.0 17 1649.0 18 1661.0 19 1483.0 20 1153.0 21 874.0 22 645.0 23 578.0 24 552.0 25 577.0 26 707.0 27 750.0 28 902.0 29 1162.0 30 1411.0 31 1798.0 32 2440.0 33 3152.0 34 4666.0 35 7144.0 36 14079.0 37 39791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.1309145480226 21.534030720338983 10.93308615819209 28.40196857344633 2 20.735125353107346 19.190280720338983 30.024055437853107 30.05053848870056 3 21.49651306497175 19.936219985875706 25.57821327683616 32.98905367231638 4 17.09922316384181 16.900600282485875 28.89742231638418 37.10275423728814 5 19.053451624293785 29.69853460451977 29.75039724576271 21.49761652542373 6 32.78160310734463 28.254104872881356 15.751897951977401 23.21239406779661 7 30.3760593220339 23.95060911016949 19.740907485875706 25.932424081920903 8 26.30429025423729 27.970515536723163 18.06254413841808 27.66265007062147 9 24.563029661016948 14.999337923728815 18.828345692090394 41.60928672316384 10-11 28.855490819209038 20.803539901129945 22.38866084039548 27.95230843926554 12-13 27.05078125 18.57068767655367 22.427833686440678 31.95069738700565 14-15 25.944010416666668 19.886012535310734 20.416225282485875 33.75375176553672 16-17 27.481682556497177 21.20464777542373 21.21788930084746 30.09578036723164 18-19 26.92995233050847 20.71691825564972 21.78672316384181 30.56640625 20-21 26.213254766949152 21.20850988700565 22.219279661016948 30.35895568502825 22-23 28.0907927259887 20.57898569915254 21.338718220338983 29.99150335451977 24-25 27.44306144067797 21.601893538135595 20.702021539548024 30.25302348163842 26-27 26.26291048728814 21.920793608757062 22.17127913135593 29.64501677259887 28-29 26.98181497175141 21.231682556497177 23.14508298022599 28.641419491525422 30-31 27.014367055084747 20.88243732344633 22.409074858757062 29.69412076271186 32-33 25.95007944915254 22.01513947740113 22.702043608757062 29.332737464689266 34-35 26.67284604519774 21.55665165960452 22.769354696327685 29.00114759887006 36-37 26.88250353107345 20.60602048022599 22.78866525423729 29.72281073446328 38-39 25.56552348163842 20.958576094632768 24.46923552259887 29.00666490112994 40-41 25.59476518361582 20.538709392655367 24.47309763418079 29.39342778954802 42-43 25.073931850282488 20.38367319915254 24.14757680084746 30.394818149717512 44-45 23.9892302259887 20.72905632062147 25.20082980225989 30.08088365112994 46-47 24.399717514124294 21.15664724576271 25.40662517655367 29.037010063559322 48-49 24.145369879943505 21.282993467514125 25.28910663841808 29.28253001412429 50-51 24.030058262711865 21.978173552259886 24.31309586864407 29.67867231638418 52-53 23.43694826977401 22.09182997881356 24.373234463276834 30.09798728813559 54-55 23.532397598870055 21.583686440677965 24.40192443502825 30.48199152542373 56-57 23.45405190677966 21.910862464689266 24.21930173022599 30.415783898305083 58-59 24.051024011299436 21.563272422316384 23.91309145480226 30.47261211158192 60-61 23.81212482344633 21.00933527542373 23.855711511299436 31.32282838983051 62-63 23.735986052259886 21.38230490819209 23.805504060734464 31.07620497881356 64-65 24.451028425141246 21.51085805084746 22.805768891242938 31.23234463276836 66-67 24.24468132062147 21.86562058615819 21.842999646892654 32.04669844632768 68-69 24.161370056497177 22.781492761299436 21.88879325564972 31.16834392655367 70-71 25.426487464689266 22.1337614759887 21.98920815677966 30.45054290254237 72-73 25.00772422316384 21.899276129943505 21.6504457980226 31.44255384887006 74-75 24.23199152542373 22.91059763418079 21.915276306497177 30.942134533898308 76 23.7939177259887 22.314177259887007 21.06395656779661 32.82794844632768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.5 16 2.0 17 2.0 18 3.0 19 7.0 20 11.0 21 12.0 22 19.5 23 46.5 24 59.5 25 53.0 26 96.5 27 173.0 28 238.0 29 270.0 30 336.5 31 478.0 32 700.0 33 847.0 34 945.0 35 1211.0 36 1483.5 37 1588.0 38 1819.5 39 2352.5 40 2809.5 41 3177.5 42 3390.0 43 3502.0 44 3719.0 45 3832.5 46 3841.0 47 3888.5 48 3780.5 49 3429.5 50 3234.0 51 3093.5 52 2641.0 53 2178.0 54 2027.0 55 1974.5 56 1768.0 57 1622.0 58 1630.0 59 1618.5 60 1677.0 61 1803.5 62 1860.0 63 2005.0 64 2223.5 65 2446.5 66 2578.0 67 2560.0 68 2708.5 69 2862.5 70 3004.0 71 3140.0 72 3115.5 73 2932.0 74 2605.5 75 2438.0 76 2297.0 77 1870.0 78 1326.5 79 1069.0 80 894.0 81 577.5 82 331.5 83 227.0 84 176.0 85 83.0 86 28.0 87 15.0 88 13.0 89 8.0 90 2.5 91 1.0 92 2.0 93 1.5 94 2.5 95 4.0 96 4.0 97 3.5 98 3.0 99 11.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 90624.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.59803142655367 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75495106727287 57.10297492937853 2 12.386530014641288 17.737023305084744 3 3.857594205132157 8.285884533898304 4 1.565847268243816 4.484463276836158 5 0.8738537412344918 3.1283103813559325 6 0.5055097480157201 2.1716101694915255 7 0.33135547507127994 1.6607079802259888 8 0.22963705016567776 1.315324858757062 9 0.1448716960776759 0.9335275423728813 >10 0.3498497341450258 3.1801730225988702 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005517302259887006 0.0 2 0.0 0.0 0.0 0.007724223163841808 0.0 3 0.0 0.0 0.0 0.00882768361581921 0.0 4 0.0 0.0 0.0 0.05075918079096045 0.0 5 0.0 0.0 0.0 0.05406956214689265 0.0 6 0.0 0.0 0.0 0.05406956214689265 0.0011034604519774012 7 0.0 0.0 0.0 0.05517302259887006 0.0011034604519774012 8 0.0 0.0 0.0 0.05517302259887006 0.0011034604519774012 9 0.0 0.0 0.0 0.05737994350282486 0.0011034604519774012 10 0.0 0.0 0.0 0.05848340395480226 0.0011034604519774012 11 0.0 0.0 0.0 0.05848340395480226 0.0011034604519774012 12 0.0 0.0 0.0 0.05848340395480226 0.0011034604519774012 13 0.0 0.0 0.0 0.05848340395480226 0.0011034604519774012 14 0.0 0.0 0.0 0.06289724576271187 0.0011034604519774012 15 0.0 0.0 0.0 0.06400070621468927 0.0011034604519774012 16 0.0 0.0 0.0 0.06510416666666667 0.0011034604519774012 17 0.0 0.0 0.0 0.06841454802259887 0.0011034604519774012 18 0.0 0.0 0.0 0.07282838983050847 0.0011034604519774012 19 0.0 0.0 0.0 0.08386299435028248 0.0011034604519774012 20 0.0 0.0 0.0 0.08496645480225988 0.0011034604519774012 21 0.0 0.0 0.0 0.0882768361581921 0.0011034604519774012 22 0.0 0.0 0.0 0.0904837570621469 0.0011034604519774012 23 0.0 0.0 0.0 0.0937941384180791 0.0011034604519774012 24 0.0 0.0 0.0 0.0993114406779661 0.0011034604519774012 25 0.0 0.0 0.0 0.1026218220338983 0.0011034604519774012 26 0.0 0.0 0.0 0.1092425847457627 0.0011034604519774012 27 0.0 0.0 0.0 0.1710363700564972 0.0011034604519774012 28 0.0 0.0 0.0 0.22510593220338984 0.0011034604519774012 29 0.0 0.0 0.0 0.2846927966101695 0.0011034604519774012 30 0.0 0.0 0.0 0.3762800141242938 0.0011034604519774012 31 0.0 0.0 0.0 0.4733845338983051 0.0011034604519774012 32 0.0 0.0 0.0 0.5660752118644068 0.0011034604519774012 33 0.0 0.0 0.0 0.7073181497175142 0.0011034604519774012 34 0.0 0.0 0.0 0.9169756355932204 0.0011034604519774012 35 0.0 0.0 0.0 1.1751853813559323 0.0011034604519774012 36 0.0 0.0 0.0 1.5613965395480225 0.0011034604519774012 37 0.0 0.0 0.0 2.054643361581921 0.0011034604519774012 38 0.0 0.0 0.0 2.5953389830508473 0.0011034604519774012 39 0.0 0.0 0.0 3.1481726694915255 0.0011034604519774012 40 0.0 0.0 0.0 3.726385946327684 0.0011034604519774012 41 0.0 0.0 0.0 4.378531073446328 0.0011034604519774012 42 0.0 0.0 0.0 4.966675494350283 0.0011034604519774012 43 0.0 0.0 0.0 5.692752471751413 0.0011034604519774012 44 0.0 0.0 0.0 6.350414901129944 0.0011034604519774012 45 0.0 0.0 0.0 7.033456920903955 0.0022069209039548023 46 0.0 0.0 0.0 7.717602401129944 0.0022069209039548023 47 0.0 0.0 0.0 8.391816737288135 0.0022069209039548023 48 0.0 0.0 0.0 8.97996115819209 0.0022069209039548023 49 0.0 0.0 0.0 9.537208686440678 0.0022069209039548023 50 0.0 0.0 0.0 10.074593926553673 0.0022069209039548023 51 0.0 0.0 0.0 10.562323446327683 0.0022069209039548023 52 0.0 0.0 0.0 11.185778601694915 0.0022069209039548023 53 0.0 0.0 0.0 11.805923375706215 0.0022069209039548023 54 0.0 0.0 0.0 12.354343220338983 0.0022069209039548023 55 0.0 0.0 0.0 12.892831920903955 0.0022069209039548023 56 0.0 0.0 0.0 13.456700211864407 0.0022069209039548023 57 0.0 0.0 0.0 13.976430084745763 0.0022069209039548023 58 0.0 0.0 0.0 14.427745409604519 0.0022069209039548023 59 0.0 0.0 0.0 14.906647245762711 0.0022069209039548023 60 0.0 0.0 0.0 15.413135593220339 0.0022069209039548023 61 0.0 0.0 0.0 15.899761652542374 0.0022069209039548023 62 0.0 0.0 0.0 16.441560734463277 0.0022069209039548023 63 0.0 0.0 0.0 16.97232521186441 0.0022069209039548023 64 0.0 0.0 0.0 17.5538488700565 0.0022069209039548023 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGGAG 15 0.002218277 70.00001 42 ATCGATA 15 0.002218277 70.00001 60 GTTTGTC 20 0.006931489 52.5 35 TAGAGGA 20 0.006931489 52.5 41 CGGAAGA 20 0.006931489 52.5 62 TGTAATC 20 0.006931489 52.5 56 TAAGAGA 40 0.0024077294 35.0 4 CTGTAAT 45 0.0042877453 31.111113 55 GAGACAG 45 0.0042877453 31.111113 7 CGGACCG 45 0.0042877453 31.111113 5 TTAGGGA 50 0.0071759117 27.999998 68 GCGGATC 50 0.0071759117 27.999998 31 AGAGACA 50 0.0071759117 27.999998 6 AGGCAAC 190 5.4387783E-10 22.105265 70 ACCTGTG 90 0.0052401735 19.444445 70 GGCAACC 135 1.3943425E-4 18.148148 70 GAGGCAA 225 9.120413E-8 17.11111 69 CGAGGCA 275 8.89484E-7 14.0 68 ACGAGGC 400 1.4188117E-10 13.999999 70 GACGAGG 500 4.836693E-9 11.2 69 >>END_MODULE