FastQCFastQC Report
Wed 25 May 2016
SRR1780208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90624
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT41204.546257062146893No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG15681.7302259887005649TruSeq Adapter, Index 2 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG11361.2535310734463279No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC10991.2127030367231637No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC3200.3531073446327684No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2270.2504855225988701TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT1380.15227754237288135No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT1290.14234639830508475No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1030.11365642655367231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA150.00221827770.0000157
ATAATGC150.00221827770.0000125
TACGGCC150.00221827770.0000168
GTAATGT150.00221827770.0000148
TAGTTGC150.00221827770.000018
AGAGTCC150.00221827770.0000131
AATGCCG150.00221827770.0000127
TATAGTT150.00221827770.000016
TAGGCGT150.00221827770.0000162
TAATGCC150.00221827770.0000126
GTTGCTA150.00221827770.000011
TTTCAGG252.3792591E-455.99999637
TTAGGTG200.00693148952.522
TTAGGCG200.00693148952.561
GCAAGCA200.00693148952.513
ATTAGGT200.00693148952.521
AGGCGTA200.00693148952.563
AGGTTTA200.00693148952.543
TGCAGTA200.00693148952.544
CAATCTT200.00693148952.54
Produced by FastQC (version 0.11.5)