FastQCFastQC Report
Wed 25 May 2016
SRR1780207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518228
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA407.0546684E-427.48129743
TTCGGTA450.001398744324.43961526
CGGTAAG450.001398744324.43961528
TAAGTAG500.002559779722.02756517
CTGTAAG500.002569861222.01266314
TTGTATA755.5099532E-520.5114482
ACGAGAG550.004470311620.00764324
TCCTGTA550.004482828519.9979812
AAGCATT550.00449537419.98832732
GTAAGTA600.00735731418.35452816
CAGTGTA600.007365527618.35097934
GGCTTGT600.007414967818.32971230
TCGGTAA600.007414967818.32971227
TAGATTG600.007456375318.3120255
GACGAAA1254.844369E-717.59822323
GAAACTC750.001291227617.59652326
GTAAGCA750.001291227617.59652330
CAGTCAT750.001298771817.5812421
CTGCCGA5050.017.44757718
ATAATAC902.2320365E-417.0912253
Produced by FastQC (version 0.11.5)