FastQCFastQC Report
Wed 25 May 2016
SRR1780207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518228
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT171083.3012496430142715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC48780.9412845311330149No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG44130.851555685914308No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG42320.8166289741194994RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC10910.21052509706152503No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT6630.1279359664086078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1550.038.333512
TCTTCTG4850.037.1957144
GTCTTCT4850.037.1957143
AATTATC301.300881E-436.66296830
CGTCTTC5650.033.09720242
CCGTCTT5700.032.03494641
TTTATAC2050.031.1308273
ATGCCGT25650.030.61975144
GTATACC300.00567743829.4012971
CTACGTA300.00574485529.33037414
TACGTAC300.00574485529.33037415
TCTTTAT2050.029.0427441
GCCGTCT6500.027.75372540
TTATCAA407.030817E-427.49722732
TATGCCG29850.026.31144143
ACAGTCG450.001397958824.44197836
TTAATAC852.3408211E-723.300762
TGCCGTC8000.022.549939
CTTAATA903.9827137E-722.0509721
GATTCTA500.002544043622.0509721
Produced by FastQC (version 0.11.5)