FastQCFastQC Report
Wed 25 May 2016
SRR1780206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38683
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC150.00220862870.0000117
CGTACAT150.00220862870.0000137
ATAACGT150.00220862870.0000117
AACTCCC150.00220862870.0000118
CGATCCC150.00220862870.0000147
TGAACTC200.006901569752.516
GGATATA200.006901569752.51
GAGCCTG200.006901569752.567
TCTACAC400.002390719735.03
GACGAGG501.6801522E-435.070
CGACGAG851.0204349E-732.94117770
GATTTTT500.0071258327.99999835
CCGACGA1202.0802E-623.33333469
TGCCGAC1252.9659295E-622.40000267
GCCGACG1407.918032E-620.068
CTGCCGA1407.918032E-620.066
CGCTGCC1704.212222E-516.47058964
GCTGCCG1806.86959E-515.55555465
ACGCTGC1901.089609E-414.73684163
GACGCTG2153.1115097E-413.02325662
Produced by FastQC (version 0.11.5)