##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780206_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38683 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.203655352480418 34.0 31.0 34.0 12.0 34.0 2 30.65147480805522 34.0 32.0 34.0 21.0 34.0 3 30.741643616058735 34.0 32.0 34.0 21.0 34.0 4 30.48207222811054 34.0 32.0 34.0 21.0 34.0 5 30.192720316418065 34.0 31.0 34.0 11.0 34.0 6 33.409275392291185 37.0 34.0 38.0 21.0 38.0 7 33.6741721169506 37.0 34.0 38.0 21.0 38.0 8 33.639815939818526 37.0 34.0 38.0 21.0 38.0 9 34.255538608691154 38.0 35.0 38.0 23.0 38.0 10-11 34.004511025515086 38.0 34.5 38.0 22.0 38.0 12-13 34.00749683323424 38.0 34.0 38.0 21.5 38.0 14-15 34.15661918672285 38.0 35.0 38.0 22.5 38.0 16-17 34.20512886797818 38.0 35.0 38.0 22.5 38.0 18-19 34.22798128376806 38.0 35.5 38.0 22.0 38.0 20-21 34.25636584546183 38.0 35.0 38.0 23.0 38.0 22-23 34.27000232660342 38.0 35.5 38.0 23.0 38.0 24-25 34.3346431248869 38.0 35.5 38.0 23.0 38.0 26-27 34.39607838068402 38.0 36.0 38.0 23.0 38.0 28-29 34.53549362769175 38.0 36.0 38.0 24.0 38.0 30-31 34.523976940775015 38.0 36.0 38.0 24.0 38.0 32-33 34.579453506708376 38.0 36.0 38.0 24.0 38.0 34-35 34.52371842928419 38.0 36.0 38.0 24.0 38.0 36-37 34.506708373187195 38.0 36.0 38.0 24.0 38.0 38-39 34.52692397177054 38.0 36.0 38.0 24.0 38.0 40-41 34.58048755267171 38.0 36.0 38.0 24.5 38.0 42-43 34.61727373781764 38.0 36.0 38.0 24.5 38.0 44-45 34.625171263862676 38.0 36.0 38.0 25.0 38.0 46-47 34.53708347336039 38.0 36.0 38.0 24.5 38.0 48-49 34.57344311454644 38.0 36.0 38.0 24.0 38.0 50-51 34.481503502830705 38.0 36.0 38.0 24.0 38.0 52-53 34.49731148049531 38.0 36.0 38.0 24.0 38.0 54-55 34.50418788615154 38.0 36.0 38.0 24.0 38.0 56-57 34.39441098156813 38.0 36.0 38.0 24.0 38.0 58-59 34.46256753612698 38.0 36.0 38.0 24.0 38.0 60-61 34.49198614378409 38.0 36.0 38.0 24.0 38.0 62-63 34.32342372618463 38.0 35.0 38.0 24.0 38.0 64-65 34.2496445467001 38.0 35.0 38.0 23.5 38.0 66-67 34.18138458754491 38.0 35.0 38.0 23.5 38.0 68-69 34.19728304423131 38.0 35.0 38.0 23.5 38.0 70-71 34.15142310575705 38.0 35.0 38.0 23.0 38.0 72-73 34.21976061835949 38.0 35.0 38.0 23.0 38.0 74-75 34.3044231316082 38.0 35.0 38.0 24.0 38.0 76 32.59129333298865 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013088166022036773 1101 2 0.31667519570813596 1101 3 0.40671013534097256 1101 4 1.0443294983126137 1101 5 0.015237311269398646 1101 6 0.030252008410315057 1101 7 -0.05346244257106747 1101 8 -0.030103782800146917 1101 9 0.08980216984307532 1101 10-11 -1.0726090958999563 1101 12-13 -0.5077605819522972 1101 14-15 -0.19704229765337544 1101 16-17 -0.3083061002925902 1101 18-19 -0.208275903787559 1101 20-21 0.04896293469231239 1101 22-23 -0.41622040976011476 1101 24-25 -0.8774491727723088 1101 26-27 -0.5923241129347474 1101 28-29 -0.352005792401755 1101 30-31 -0.610716375749206 1101 32-33 -0.3521881879274176 1101 34-35 -0.012019329956771685 1101 36-37 -0.598955084558213 1101 38-39 -0.035332702993088105 1101 40-41 0.2581208565112192 1101 42-43 -0.628133701997541 1101 44-45 0.5480874626411847 1101 46-47 0.2067221640211585 1101 48-49 -0.22528377132191935 1101 50-51 0.214561768174093 1101 52-53 -0.3268559162004223 1101 54-55 -0.9214279433335335 1101 56-57 -0.10571813328810009 1101 58-59 0.12412647655507669 1101 60-61 0.1730146727195674 1101 62-63 0.29377504449663405 1101 64-65 -0.40491744282111597 1101 66-67 -0.5628047278340773 1101 68-69 -0.4851665494451325 1101 70-71 -0.07382598111707495 1101 72-73 0.020978961749669622 1101 74-75 -0.3082718659591137 1101 76 -0.14910928078526808 1102 1 0.15546497761624067 1102 2 -0.35694799269766264 1102 3 -0.2837826182822134 1102 4 0.49714109251551264 1102 5 0.19355615184910846 1102 6 0.4801650518885765 1102 7 0.5767404559796603 1102 8 0.18282375343173385 1102 9 0.3028456481039399 1102 10-11 0.7970720635203321 1102 12-13 0.6143553600766865 1102 14-15 0.4746388617669197 1102 16-17 -0.02045102782881969 1102 18-19 -0.1044208313237931 1102 20-21 0.2321803259966515 1102 22-23 -0.14642330831083683 1102 24-25 0.1700001025900093 1102 26-27 0.26292226387684536 1102 28-29 0.8101391351344773 1102 30-31 0.8124720300478927 1102 32-33 0.20526108743490568 1102 34-35 0.5711400903330812 1102 36-37 0.05168259660121066 1102 38-39 0.6057977317895222 1102 40-41 0.18606288549672456 1102 42-43 0.41125760235028963 1102 44-45 0.2214787669890157 1102 46-47 0.23550477271681558 1102 48-49 0.3504553591128641 1102 50-51 0.6102429275943777 1102 52-53 0.792941185248857 1102 54-55 0.8359633610142936 1102 56-57 0.456571721784357 1102 58-59 0.649169954815946 1102 60-61 0.5330726437340587 1102 62-63 0.17896345029373606 1102 64-65 0.1484738615267105 1102 66-67 0.2971662866586726 1102 68-69 0.31857258098964536 1102 70-71 0.4908696710568421 1102 72-73 0.7879644689960443 1102 74-75 0.12219190215682829 1102 76 -0.270790440205559 1103 1 0.7692280261618976 1103 2 -0.4914366924037523 1103 3 -0.014045109414269064 1103 4 0.8650847430678574 1103 5 0.7473212273533143 1103 6 0.17688137903969192 1103 7 -0.2237561488647728 1103 8 0.377057056360691 1103 9 -0.6556523756114743 1103 10-11 0.26428600899514976 1103 12-13 0.26756808937637544 1103 14-15 0.06947518486411042 1103 16-17 0.32430928432280126 1103 18-19 -0.14077940029105207 1103 20-21 -0.07604405831468597 1103 22-23 0.1855418280021226 1103 24-25 0.19617320485006218 1103 26-27 -0.1464220150326554 1103 28-29 -0.1342715266674901 1103 30-31 -0.468059033091869 1103 32-33 -0.37520916694838746 1103 34-35 0.049071579134135845 1103 36-37 -0.38449354609667097 1103 38-39 -0.5371788568392404 1103 40-41 -0.2865225001321434 1103 42-43 -0.10158824745208506 1103 44-45 0.1264650850188147 1103 46-47 -0.18704007374108045 1103 48-49 -0.27443062446877065 1103 50-51 -0.07464102902871161 1103 52-53 -0.06914962249413037 1103 54-55 0.28640422449863223 1103 56-57 0.038030118460149254 1103 58-59 -0.34079660036800874 1103 60-61 -0.16567064196575387 1103 62-63 -0.39002915130756577 1103 64-65 0.3996140257103491 1103 66-67 -0.014301231330584585 1103 68-69 0.39998030370171733 1103 70-71 -0.11083297412406523 1103 72-73 0.37261532538602893 1103 74-75 0.247280581593337 1103 76 0.43332428564829684 1104 1 1.0470192005047956 1104 2 0.10012347157020329 1104 3 -0.18997951983143935 1104 4 1.2755019121057174 1104 5 -0.21703855079956824 1104 6 0.8959071808241106 1104 7 0.9377099712220343 1104 8 0.5723789758205413 1104 9 0.24842285949824827 1104 10-11 0.5249081454793512 1104 12-13 -0.07962265091781262 1104 14-15 0.6938542540707644 1104 16-17 0.6500387272936194 1104 18-19 0.1695171996607172 1104 20-21 0.0238594864164412 1104 22-23 0.6477795902398853 1104 24-25 0.6461370341242443 1104 26-27 0.37291726637559464 1104 28-29 0.39999420759824034 1104 30-31 0.823766382871483 1104 32-33 0.7176049155208588 1104 34-35 0.6020496355604692 1104 36-37 0.2873897430279939 1104 38-39 0.7787362625241556 1104 40-41 -0.043672246937056514 1104 42-43 -0.08716818475615895 1104 44-45 0.3799495316067052 1104 46-47 0.7921014743659853 1104 48-49 0.39940588385049836 1104 50-51 0.2535962509327092 1104 52-53 0.09231649759268379 1104 54-55 0.5392617118388756 1104 56-57 0.3345577287808581 1104 58-59 0.322333373106801 1104 60-61 0.7834974313402583 1104 62-63 0.5850853893242203 1104 64-65 1.0448756606271559 1104 66-67 0.33057458251074934 1104 68-69 0.626143795382454 1104 70-71 0.13734643267603275 1104 72-73 0.643461720370361 1104 74-75 0.5721419271443438 1104 76 0.8875803743871415 1105 1 0.16736631171740157 1105 2 0.7741586394167435 1105 3 0.5088558307052118 1105 4 0.6997996969881122 1105 5 0.2285883046468804 1105 6 0.9784374388738968 1105 7 1.3964448421971483 1105 8 0.862399528458127 1105 9 0.8633121036179077 1105 10-11 0.9242915663516982 1105 12-13 0.32140498096160997 1105 14-15 0.7359643248631897 1105 16-17 0.3171773434160272 1105 18-19 0.5444658180667403 1105 20-21 0.34742651085124976 1105 22-23 0.9080444909021352 1105 24-25 0.5735177146449075 1105 26-27 0.43448383408512115 1105 28-29 0.4410538102472543 1105 30-31 1.1131246838534423 1105 32-33 0.22963962664211834 1105 34-35 0.43137139852005646 1105 36-37 0.037309816104041715 1105 38-39 0.5371970983314185 1105 40-41 0.785352644590688 1105 42-43 0.6657206026382241 1105 44-45 0.1034252109855558 1105 46-47 0.19310626998142055 1105 48-49 0.7740804670886803 1105 50-51 0.7622968675118358 1105 52-53 0.806018255985002 1105 54-55 0.5589694076598448 1105 56-57 0.38230835677811825 1105 58-59 0.590431112316665 1105 60-61 1.0927232819910913 1105 62-63 0.3024637862184889 1105 64-65 0.0502878551921242 1105 66-67 0.7143608350798303 1105 68-69 0.6859062982369792 1105 70-71 0.2629687208696865 1105 72-73 0.5553365776437076 1105 74-75 0.2964168757627448 1105 76 0.16490396424784137 1106 1 -0.5369549994455873 1106 2 -1.3731089769537377 1106 3 -0.41677187904751634 1106 4 -0.12067769593199174 1106 5 -0.9961008182270064 1106 6 -0.12899979015083574 1106 7 -1.4742969749451689 1106 8 -1.3757728475483475 1106 9 -0.35480214670369037 1106 10-11 -0.6715443476985214 1106 12-13 -0.42534331576524664 1106 14-15 -0.6504523695958184 1106 16-17 0.015233468052734622 1106 18-19 -0.43923993256453286 1106 20-21 -0.36361260487603886 1106 22-23 -0.38315566155868197 1106 24-25 0.13869471211977924 1106 26-27 -0.4169859834383445 1106 28-29 -0.19496982117873785 1106 30-31 -0.8358962318643108 1106 32-33 -0.10482128145259395 1106 34-35 0.01048426716553763 1106 36-37 -0.005688897507852175 1106 38-39 -0.24664205551107443 1106 40-41 0.12407769104358835 1106 42-43 -0.7611696732205644 1106 44-45 -0.7728909546250051 1106 46-47 0.13705309927296128 1106 48-49 -0.12991686484709675 1106 50-51 0.031914286159704375 1106 52-53 0.5004534363175637 1106 54-55 -0.16560060520403397 1106 56-57 -0.17055266566220695 1106 58-59 -0.3969670486247878 1106 60-61 0.16700028423036173 1106 62-63 -0.5323544519062438 1106 64-65 -0.28114765864845026 1106 66-67 -1.0395817062513473 1106 68-69 -0.3128787796609629 1106 70-71 -0.3408475638508861 1106 72-73 0.03415881786477115 1106 74-75 -0.1427179091245776 1106 76 0.4568187767686851 1107 1 -0.11297065750737545 1107 2 -0.5003836278212788 1107 3 -0.8354663352472613 1107 4 -0.5487881487462083 1107 5 0.0959431936223396 1107 6 -0.8702185798249786 1107 7 0.32812579272304987 1107 8 0.3761315113174959 1107 9 0.9927433463136595 1107 10-11 0.391008551158869 1107 12-13 0.640357065106528 1107 14-15 0.9141635846995726 1107 16-17 0.7374880173544724 1107 18-19 0.646165272683028 1107 20-21 0.8985805817511334 1107 22-23 0.9735148843575274 1107 24-25 0.4839920036982761 1107 26-27 0.3622935341240776 1107 28-29 0.7286706781864751 1107 30-31 0.1451659771919651 1107 32-33 0.3942529885431796 1107 34-35 0.01865714063146129 1107 36-37 -0.11780802573467497 1107 38-39 0.2122555323010289 1107 40-41 0.9671796800406227 1107 42-43 0.24533688623775163 1107 44-45 0.07761687440589782 1107 46-47 0.5323398110799857 1107 48-49 0.5566574051486697 1107 50-51 0.1968264740564365 1107 52-53 -0.09815003384748167 1107 54-55 1.1296014684311686 1107 56-57 1.1906936314177798 1107 58-59 0.6883323589080135 1107 60-61 1.2813676138960375 1107 62-63 1.0226868092025185 1107 64-65 0.6406413807082956 1107 66-67 1.1178717427541542 1107 68-69 0.296833450554864 1107 70-71 0.6284975482946962 1107 72-73 0.8288319029261402 1107 74-75 0.8548751928644194 1107 76 0.4110083662735562 1108 1 0.17632954533238276 1108 2 0.15529588536330508 1108 3 0.19622737672028379 1108 4 1.6117088086574434 1108 5 1.1243407595452588 1108 6 1.3407347670310088 1108 7 1.1204685919116955 1108 8 0.25341345857916053 1108 9 0.7087676870844533 1108 10-11 0.8964598696172814 1108 12-13 0.8807221766683853 1108 14-15 0.8024749437259402 1108 16-17 0.35348700315568493 1108 18-19 0.4376206479365763 1108 20-21 0.5635146588302362 1108 22-23 0.6719175212743664 1108 24-25 0.2625163444690699 1108 26-27 0.32033105947904517 1108 28-29 0.6344769662189336 1108 30-31 0.4823870725266488 1108 32-33 0.6555359500036175 1108 34-35 0.7434289459053005 1108 36-37 0.09859663957972487 1108 38-39 0.1813224694207065 1108 40-41 0.40201740823535204 1108 42-43 0.12059043593349372 1108 44-45 -0.04332633046225709 1108 46-47 0.25761871574530204 1108 48-49 0.06578519419532114 1108 50-51 0.41221694058788216 1108 52-53 -0.27233867482111407 1108 54-55 0.4720203325285297 1108 56-57 0.2604197977463798 1108 58-59 0.2835402696585163 1108 60-61 0.41194570720232804 1108 62-63 0.16784401001387295 1108 64-65 0.21987566062715302 1108 66-67 0.7779883756141999 1108 68-69 0.9692472436583088 1108 70-71 0.5287257430208641 1108 72-73 0.8322548238186371 1108 74-75 0.30403847886847757 1108 76 0.3841320985250718 1109 1 0.36598471774617636 1109 2 -0.15504894222290133 1109 3 -0.2692898646590258 1109 4 -0.4400153292735922 1109 5 -0.4791075163168088 1109 6 -0.35754109503795917 1109 7 0.1635720401875531 1109 8 -0.9793451621104907 1109 9 -0.07571507153623713 1109 10-11 -0.013022889003401872 1109 12-13 0.2427911421856308 1109 14-15 0.3369577023466306 1109 16-17 0.31900424453500165 1109 18-19 0.5367585789710603 1109 20-21 0.2617905208992042 1109 22-23 -0.3237721338980464 1109 24-25 -0.6728284831171365 1109 26-27 0.00998623189283876 1109 28-29 -0.14138510274658245 1109 30-31 -0.31502672057679604 1109 32-33 -0.5384295672377633 1109 34-35 0.20894618728461012 1109 36-37 0.2106656050969633 1109 38-39 0.45718453838622963 1109 40-41 0.03563809789052641 1109 42-43 0.33610767731281044 1109 44-45 0.1161564281584262 1109 46-47 0.2058945778142629 1109 48-49 1.0183714010918763 1109 50-51 0.44669969920856545 1109 52-53 -0.4792352265452493 1109 54-55 0.18322722908025924 1109 56-57 0.5276611770567214 1109 58-59 -0.13025283378975416 1109 60-61 0.2697043278919793 1109 62-63 0.2842233203587057 1109 64-65 0.3319446261444057 1109 66-67 -0.006494383006490523 1109 68-69 0.15459207124451524 1109 70-71 0.3864843637105153 1109 72-73 0.504668616922082 1109 74-75 0.5583488236960648 1109 76 -0.21931617733699227 1110 1 1.0558771568477248 1110 2 0.14896877368978423 1110 3 0.5334624289189556 1110 4 -0.8292117860910544 1110 5 -0.4623406703819768 1110 6 1.4025822836396742 1110 7 0.6018036124748036 1110 8 0.4369787860071881 1110 9 -0.12854645401014153 1110 10-11 0.4667303536413243 1110 12-13 0.4035788675889833 1110 14-15 0.8163155005117702 1110 16-17 0.5635471107165912 1110 18-19 0.2869167567628992 1110 20-21 0.8619170631326725 1110 22-23 0.3285135351940127 1110 24-25 0.06167009328273565 1110 26-27 0.5676024925698329 1110 28-29 0.31100338191016874 1110 30-31 0.27185243159024 1110 32-33 0.27812373867809015 1110 34-35 0.6583292938964433 1110 36-37 0.7513568420472936 1110 38-39 0.8414379392087454 1110 40-41 0.8224144344928632 1110 42-43 0.22368281176393623 1110 44-45 0.3114269121824762 1110 46-47 0.4452175768651969 1110 48-49 0.5236703571184478 1110 50-51 0.35558929110986526 1110 52-53 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0.41047247332191716 2116 36-37 0.011471144949986467 2116 38-39 0.08388041176101524 2116 40-41 0.2094651188062997 2116 42-43 0.2471449865270472 2116 44-45 0.6598251675592195 2116 46-47 0.9523615082834525 2116 48-49 0.7508473762190633 2116 50-51 0.6078076698134325 2116 52-53 1.2109145756028497 2116 54-55 0.6349654992894145 2116 56-57 -0.2055214119766262 2116 58-59 0.5476018863911349 2116 60-61 0.1162277874736688 2116 62-63 -0.5302905292738629 2116 64-65 -0.07711416413259542 2116 66-67 -0.7157227606209631 2116 68-69 -0.7154616314123459 2116 70-71 0.7838133631452209 2116 72-73 0.11553633879885439 2116 74-75 0.594810101254005 2116 76 0.014431702821291026 2117 1 -1.4494024000156998 2117 2 -0.622192728820167 2117 3 -0.7126860910741186 2117 4 -2.511745973385498 2117 5 -0.9423475943909807 2117 6 -2.156012142533079 2117 7 -1.0457643293635215 2117 8 -0.9149339714793925 2117 9 -2.3932166980814564 2117 10-11 -0.8783449449565595 2117 12-13 -2.8094209593107884 2117 14-15 -1.3941743731250682 2117 16-17 -2.1546457229340916 2117 18-19 -1.6254079792592577 2117 20-21 -1.6962446124775 2117 22-23 -1.6182581456091754 2117 24-25 -1.7946567199421182 2117 26-27 -1.6594184525573894 2117 28-29 -1.4849114527791158 2117 30-31 -0.8295843002775101 2117 32-33 -1.5282636596255301 2117 34-35 -0.8977551790133731 2117 36-37 -0.6493701788978328 2117 38-39 -1.0625779860119522 2117 40-41 -1.7978036717906676 2117 42-43 -1.376888418978666 2117 44-45 -2.1751578203776845 2117 46-47 -1.8266363265448788 2117 48-49 -2.242038488303052 2117 50-51 -0.9472495525806295 2117 52-53 -1.8345540294079683 2117 54-55 -0.8153902074844837 2117 56-57 -0.7608879446088608 2117 58-59 -0.8393452215581334 2117 60-61 -0.13400419520495888 2117 62-63 -0.8546023139939294 2117 64-65 -0.3708042352739511 2117 66-67 -0.9825028410416223 2117 68-69 -1.1418457947281553 2117 70-71 -2.2782033396076393 2117 72-73 -3.255171981646555 2117 74-75 -2.0964982810534494 2117 76 -2.850090412860741 2118 1 1.7134359921089946 2118 2 0.2662404130994389 2118 3 2.100275352732279 2118 4 0.48989471570391885 2118 5 1.961672093878093 2118 6 -0.15026973072011884 2118 7 1.5694940791680665 2118 8 0.7552875215476789 2118 9 0.8898021698430725 2118 10-11 -0.1884351828564732 2118 12-13 -0.19723884282186077 2118 14-15 0.391305528433584 2118 16-17 0.45056346492480515 2118 18-19 1.855724096212441 2118 20-21 0.28652815208361204 2118 22-23 1.5456056771963986 2118 24-25 1.1663769141842124 2118 26-27 1.7339367566304702 2118 28-29 1.1217333380330246 2118 30-31 1.4501531894681818 2118 32-33 1.049463985985632 2118 34-35 1.7269371917823548 2118 36-37 1.3487840458765703 2118 38-39 0.062319470919959485 2118 40-41 -0.0929226217496506 2118 42-43 0.791692384958985 2118 44-45 -0.13721688518489827 2118 46-47 1.463765642282027 2118 48-49 0.024368402591129268 2118 50-51 0.7986487246958234 2118 52-53 -0.03676895967868887 2118 54-55 -0.022732291159620388 2118 56-57 -0.14850074198376007 2118 58-59 0.6926482156855087 2118 60-61 0.35553641185000373 2118 62-63 -0.5963988685468493 2118 64-65 0.3876042963093198 2118 66-67 0.2935430982528757 2118 68-69 -0.6814274190103546 2118 70-71 1.4691305406220607 2118 72-73 -0.39319495129380755 2118 74-75 0.777989003606109 2118 76 -0.0026744981765745024 2119 1 0.4456735318756948 2119 2 -0.7252760238040601 2119 3 -0.15953376709805056 2119 4 -1.4528412333947038 2119 5 -1.4370553716574328 2119 6 0.31844713036154104 2119 7 -0.6881941498881403 2119 8 -0.5352745145074636 2119 9 -0.183368561864242 2119 10-11 -1.694267632485321 2119 12-13 -1.5379069234157114 2119 14-15 0.1357381901515069 2119 16-17 -0.6334768319999071 2119 18-19 -1.2418368793973116 2119 20-21 -1.5830370653076926 2119 22-23 -2.674171629272312 2119 24-25 -1.120473563016212 2119 26-27 -0.3662753324469463 2119 28-29 -0.7658594509383363 2119 30-31 -0.6399846684321346 2119 32-33 -1.1035052610981424 2119 34-35 -0.31123884215189435 2119 36-37 -1.0936867918752853 2119 38-39 -1.883332702993087 2119 40-41 -1.9542693873912214 2119 42-43 -0.7634507751682662 2119 44-45 -1.4837174154075896 2119 46-47 -2.071229055491038 2119 48-49 -1.6042106005902141 2119 50-51 -1.5457309147527383 2119 52-53 -1.4604168918101763 2119 54-55 -0.7838669677237817 2119 56-57 -1.896644962556401 2119 58-59 -1.4102149868595646 2119 60-61 -1.6192780102072604 2119 62-63 -0.9869078823326376 2119 64-65 -1.1786735403820927 2119 66-67 -1.8766583863706572 2119 68-69 -1.4775567933475742 2119 70-71 -2.215386956726828 2119 72-73 -2.686142989469843 2119 74-75 -1.7368572318127633 2119 76 -1.718475134443807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 41.0 14 165.0 15 406.0 16 495.0 17 565.0 18 518.0 19 428.0 20 350.0 21 274.0 22 221.0 23 221.0 24 212.0 25 250.0 26 287.0 27 359.0 28 437.0 29 490.0 30 687.0 31 840.0 32 1060.0 33 1668.0 34 2156.0 35 3639.0 36 7200.0 37 15713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.313160819998444 18.020836026161362 10.560194400641109 29.10580875319908 2 22.97650130548303 20.02688519504692 30.424217356461497 26.572396143008557 3 19.160871700747098 23.116097510534342 28.534498358452034 29.188532430266523 4 14.3292919370266 17.95879326836078 32.05283974872683 35.65907504588579 5 14.344802626476746 30.8145697076235 32.93953416229351 21.901093503606237 6 27.575420727451334 34.937827986453996 18.70072124705943 18.786030039035236 7 27.93475169971305 27.87787917172918 21.782178217821784 22.405190910735982 8 25.08854018561125 31.290230850761315 21.068686503115064 22.55254246051237 9 23.824418995424345 18.449965100948738 20.58009978543546 37.14551611819145 10-11 24.63226740428612 23.49869451697128 26.43667761031978 25.43236046842282 12-13 24.66199622573223 21.63999689786211 25.604270609828607 28.093736266577054 14-15 23.259571387948196 22.845953002610965 23.551689372592612 30.34278623684823 16-17 23.81149342088256 25.37807305534731 24.80288498823773 26.007548535532404 18-19 22.496962489982682 25.38841351498074 25.72577101052142 26.38885298451516 20-21 22.690846108109504 25.415557221518497 25.995915518444797 25.897681151927205 22-23 23.81795620815345 24.791251971150118 25.55515342656981 25.83563839412662 24-25 23.06181009745883 25.72577101052142 25.325078199725976 25.88734069229377 26-27 22.242328671509448 26.15877775767133 26.00237830571569 25.596515265103537 28-29 23.140656102163742 25.020034640539773 26.860636455290436 24.97867280200605 30-31 23.11997518289688 24.916630044205466 26.32034743944368 25.64304733345397 32-33 22.44008996199881 25.940335547915105 26.160070315125505 25.45950417496058 34-35 22.92092133495334 25.484062766589975 26.27898560090996 25.316030297546728 36-37 23.100586821084196 24.885608665305174 26.326810226714574 25.686994286896052 38-39 22.480159243078354 25.16867874777034 27.077786107592484 25.273375901558826 40-41 22.36253651474808 24.703358064265956 27.623245353255953 25.31086006773001 42-43 22.014838559573974 25.24881730992943 26.624098441175708 26.11224568932089 44-45 21.87394979706848 24.885608665305174 27.25615903626916 25.984282501357185 46-47 21.96055114649846 25.080784840886178 27.235478117002298 25.72318589561306 48-49 22.144094304991857 24.475867952330482 27.46038311402942 25.919654628648242 50-51 22.025179019207403 25.255280097200323 27.11397771630949 25.605563167282785 52-53 21.53012951425691 25.03166765762738 27.10234469922188 26.33585812889383 54-55 21.42543236046842 24.859757516221595 27.037716826512938 26.677093296797043 56-57 21.481012330998112 24.845539384225628 27.1437065377556 26.52974174702066 58-59 21.453868624460355 25.04459323216917 26.7649872036812 26.73655093968927 60-61 21.36209704521366 24.663288783186413 27.18248326138097 26.792130910218958 62-63 20.931675412972105 25.291471705917328 26.830907633844326 26.94594524726624 64-65 21.870072124705946 24.925677946384717 26.461236201949177 26.743013726960164 66-67 21.289713827779643 25.546105524390562 25.538350179665485 27.62583046816431 68-69 20.86187731044645 25.615903626916214 25.782643538505283 27.73957552413205 70-71 22.104025023912314 24.970917457280976 25.949383450094356 26.97567406871235 72-73 22.344440710389577 25.035545329989915 25.400046532068348 27.219967427552156 74-75 22.063955742832768 25.19970012667063 25.472429749502368 27.263914380994237 76 22.291445854768245 25.427190238606105 24.44226145852183 27.83910244810382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.5 16 2.0 17 3.0 18 3.5 19 7.5 20 12.0 21 13.0 22 13.5 23 18.5 24 31.5 25 40.0 26 66.5 27 109.0 28 152.0 29 179.0 30 219.5 31 325.0 32 471.5 33 553.0 34 630.5 35 807.5 36 984.5 37 1062.0 38 1167.0 39 1504.5 40 1874.0 41 2055.0 42 2099.0 43 2156.0 44 2367.0 45 2456.0 46 2391.0 47 2310.5 48 2223.5 49 2059.0 50 1901.0 51 1805.5 52 1500.5 53 1199.0 54 1107.0 55 1066.0 56 925.5 57 741.0 58 656.0 59 607.0 60 508.5 61 472.5 62 486.0 63 485.0 64 487.5 65 535.5 66 591.0 67 602.0 68 604.5 69 620.5 70 609.0 71 584.0 72 548.5 73 442.5 74 339.0 75 306.0 76 257.5 77 151.5 78 70.5 79 47.0 80 35.0 81 17.5 82 9.0 83 6.0 84 4.5 85 2.5 86 3.0 87 4.0 88 3.0 89 2.0 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.93643202440349 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53094208548947 58.73639583279476 2 11.64670021974906 16.98937517772665 3 3.852697242503721 8.430059716154382 4 1.7615368256893742 5.139208437815061 5 0.9002622811370242 3.283095933614249 6 0.48911887715318636 2.140475144120156 7 0.27645849578223575 1.4114727399632914 8 0.21620472106046643 1.261536075278546 9 0.10278585099595945 0.6747149910813536 >10 0.2232934004394981 1.933665951451542 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0025851149083576763 0.0 6 0.0 0.0 0.0 0.0025851149083576763 0.0 7 0.0 0.0 0.0 0.0025851149083576763 0.0 8 0.0 0.0 0.0 0.0025851149083576763 0.0 9 0.0 0.0 0.0 0.0025851149083576763 0.0 10 0.0 0.0 0.0 0.0025851149083576763 0.0 11 0.0 0.0 0.0 0.0025851149083576763 0.0 12 0.0 0.0 0.0 0.0025851149083576763 0.0 13 0.0 0.0 0.0 0.0025851149083576763 0.0 14 0.0 0.0 0.0 0.0025851149083576763 0.0 15 0.0 0.0 0.0 0.0025851149083576763 0.0 16 0.0 0.0 0.0 0.0025851149083576763 0.0 17 0.0 0.0 0.0 0.0025851149083576763 0.0 18 0.0 0.0 0.0 0.0025851149083576763 0.0 19 0.0 0.0 0.0 0.010340459633430705 0.0 20 0.0 0.0 0.0 0.010340459633430705 0.0 21 0.0 0.0 0.0 0.010340459633430705 0.0 22 0.0 0.0 0.0 0.018095804358503737 0.0 23 0.0 0.0 0.0 0.018095804358503737 0.0 24 0.0 0.0 0.0 0.018095804358503737 0.0 25 0.0 0.0 0.0 0.018095804358503737 0.0 26 0.0 0.0 0.0 0.02068091926686141 0.0 27 0.0 0.0 0.0 0.023266034175219087 0.0 28 0.0 0.0 0.0 0.02843626399193444 0.0 29 0.0 0.0 0.0 0.046532068350438174 0.0 30 0.0 0.0 0.0 0.062042757800584235 0.0 31 0.0 0.0 0.0 0.06462787270894191 0.0 32 0.0 0.0 0.0 0.0775534472507303 0.0 33 0.0 0.0 0.0 0.09564925160923403 0.0 34 0.0 0.0 0.0 0.12408551560116847 0.0 35 0.0 0.0 0.0 0.175787813768322 0.0 36 0.0 0.0 0.0 0.23266034175219089 0.0 37 0.0 0.0 0.0 0.3283095933614249 0.0 38 0.0 0.0 0.0 0.46532068350438177 0.0 39 0.0 0.0 0.0 0.6281829227309154 0.0 40 0.0 0.0 0.0 0.863428379391464 0.0 41 0.0 0.0 0.0 1.222759351653181 0.0 42 0.0 0.0 0.0 1.6337926220820516 0.0 43 0.0 0.0 0.0 2.0034640539771993 0.0 44 0.0 0.0 0.0 2.4041568647726392 0.0 45 0.0 0.0 0.0 2.7609027221259983 0.0 46 0.0 0.0 0.0 3.1254039242044307 0.0 47 0.0 0.0 0.0 3.588139492800455 0.0 48 0.0 0.0 0.0 3.9552258097872453 0.0 49 0.0 0.0 0.0 4.3610888503994 0.0 50 0.0 0.0 0.0 4.720419822661118 0.0 51 0.0 0.0 0.0 5.170229816715353 0.0 52 0.0 0.0 0.0 5.6743272238451 0.0 53 0.0 0.0 0.0 6.284414342217511 0.0 54 0.0 0.0 0.0 6.873820541323062 0.0 55 0.0 0.0 0.0 7.396013752811313 0.0 56 0.0 0.0 0.0 7.850993976682264 0.0 57 0.0 0.0 0.0 8.368016958353799 0.0 58 0.0 0.0 0.0 8.815241837499677 0.0 59 0.0 0.0 0.0 9.226275107928547 0.0 60 0.0 0.0 0.0 9.606286999457126 0.0 61 0.0 0.0 0.0 9.98112866116899 0.0 62 0.0 0.0 0.0 10.474885608665305 0.0 63 0.0 0.0 0.0 11.061706692862497 0.0 64 0.0 0.0 0.0 11.612336168342683 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACTCC 15 0.002208628 70.00001 17 CGTACAT 15 0.002208628 70.00001 37 ATAACGT 15 0.002208628 70.00001 17 AACTCCC 15 0.002208628 70.00001 18 CGATCCC 15 0.002208628 70.00001 47 TGAACTC 20 0.0069015697 52.5 16 GGATATA 20 0.0069015697 52.5 1 GAGCCTG 20 0.0069015697 52.5 67 TCTACAC 40 0.0023907197 35.0 3 GACGAGG 50 1.6801522E-4 35.0 70 CGACGAG 85 1.0204349E-7 32.941177 70 GATTTTT 50 0.00712583 27.999998 35 CCGACGA 120 2.0802E-6 23.333334 69 TGCCGAC 125 2.9659295E-6 22.400002 67 GCCGACG 140 7.918032E-6 20.0 68 CTGCCGA 140 7.918032E-6 20.0 66 CGCTGCC 170 4.212222E-5 16.470589 64 GCTGCCG 180 6.86959E-5 15.555554 65 ACGCTGC 190 1.089609E-4 14.736841 63 GACGCTG 215 3.1115097E-4 13.023256 62 >>END_MODULE