FastQCFastQC Report
Wed 25 May 2016
SRR1780206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38683
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG12643.2675852441641027RNA PCR Primer, Index 23 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5151.3313341778042034No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1270.3283095933614249No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1070.2766072951942714No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT980.2533412610190523RNA PCR Primer, Index 47 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC510.13184086032624148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA150.00220862870.000011
AGGTATC150.00220862870.0000123
GACAGCC150.00220862870.000019
ACTATAA150.00220862870.0000133
GGCGTCG150.00220862870.0000115
TATCTTT150.00220862870.0000126
GTCCGTA150.00220862870.0000113
CATAAGT150.00220862870.000013
CGTAAAG150.00220862870.0000116
TCTTTAT150.00220862870.000011
CTTTATA150.00220862870.000012
TTCCTGT150.00220862870.0000170
TAAAAGC200.006901569752.537
TATTGTG200.006901569752.544
CCGTAAA200.006901569752.515
GTATCTT200.006901569752.525
CTTTACT200.006901569752.529
CAACTAT200.006901569752.545
GTGGAGT350.001240775240.08
AGAGACA400.002390719735.06
Produced by FastQC (version 0.11.5)