##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780206_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38683 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.27448750096942 34.0 34.0 34.0 33.0 34.0 2 33.472171238011526 34.0 34.0 34.0 34.0 34.0 3 33.52788046428664 34.0 34.0 34.0 34.0 34.0 4 33.577023498694516 34.0 34.0 34.0 34.0 34.0 5 33.53679911072047 34.0 34.0 34.0 34.0 34.0 6 37.07031512550733 38.0 37.0 38.0 35.0 38.0 7 37.043559186205826 38.0 37.0 38.0 35.0 38.0 8 36.80862394333428 38.0 37.0 38.0 34.0 38.0 9 36.90329085127834 38.0 37.0 38.0 35.0 38.0 10-11 36.800028436263986 38.0 37.0 38.0 34.0 38.0 12-13 36.790114520590436 38.0 37.0 38.0 34.0 38.0 14-15 36.719605511464984 38.0 37.0 38.0 34.0 38.0 16-17 36.74808055218054 38.0 37.0 38.0 34.0 38.0 18-19 36.711410697205494 38.0 37.0 38.0 34.0 38.0 20-21 36.7470465062172 38.0 37.0 38.0 34.0 38.0 22-23 36.68801540728485 38.0 37.0 38.0 34.0 38.0 24-25 36.69552516609363 38.0 37.0 38.0 34.0 38.0 26-27 36.54088359227568 38.0 37.0 38.0 34.0 38.0 28-29 36.506475712845436 38.0 37.0 38.0 34.0 38.0 30-31 36.407853579091594 38.0 37.0 38.0 34.0 38.0 32-33 36.22181578471164 38.0 37.0 38.0 34.0 38.0 34-35 36.38268748545873 38.0 37.0 38.0 34.0 38.0 36-37 36.45562650259804 38.0 37.0 38.0 34.0 38.0 38-39 36.174197968099676 38.0 37.0 38.0 34.0 38.0 40-41 36.27623245353256 38.0 37.0 38.0 34.0 38.0 42-43 36.296396349817755 38.0 37.0 38.0 34.0 38.0 44-45 36.335483287232115 38.0 37.0 38.0 34.0 38.0 46-47 36.22802006049169 38.0 37.0 38.0 34.0 38.0 48-49 36.23951089625934 38.0 37.0 38.0 34.0 38.0 50-51 36.04614430111418 38.0 37.0 38.0 33.0 38.0 52-53 36.10085825814957 38.0 37.0 38.0 34.0 38.0 54-55 36.01373988573792 38.0 37.0 38.0 33.0 38.0 56-57 36.04966005738955 38.0 37.0 38.0 33.5 38.0 58-59 35.9020112193987 38.0 37.0 38.0 32.0 38.0 60-61 35.97510534343252 38.0 37.0 38.0 32.0 38.0 62-63 35.88030917974304 38.0 36.5 38.0 31.5 38.0 64-65 35.67598169738645 38.0 36.5 38.0 31.5 38.0 66-67 35.087583693095155 38.0 36.0 38.0 27.5 38.0 68-69 35.07268050564848 38.0 36.0 38.0 27.0 38.0 70-71 34.72891192513507 38.0 35.5 38.0 26.0 38.0 72-73 34.55738955096554 38.0 35.0 38.0 24.5 38.0 74-75 34.434687071840344 38.0 35.0 38.0 24.0 38.0 76 33.42752630354419 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.14656941829452563 1101 2 -0.05192032989994999 1101 3 -0.1080703840007331 1101 4 0.17951501414320603 1101 5 -0.22561797498333647 1101 6 -0.3666898311824056 1101 7 -0.39611892483179645 1101 8 -0.380885059728584 1101 9 -0.3428307753896789 1101 10-11 0.025309479266780954 1101 12-13 0.07208057071954954 1101 14-15 0.2955417755943941 1101 16-17 0.30605544971476917 1101 18-19 -0.10868687986877035 1101 20-21 0.12501175478691096 1101 22-23 0.19085330230350195 1101 24-25 0.15009095417601515 1101 26-27 -0.12616713473405383 1101 28-29 -0.3154636279013445 1101 30-31 0.3113095850222294 1101 32-33 -0.6044030576795265 1101 34-35 -0.4403183701881517 1101 36-37 0.11184370621002415 1101 38-39 -0.2585848975934937 1101 40-41 0.058159899719946395 1101 42-43 -0.16274179816711154 1101 44-45 -0.11967191138483457 1101 46-47 -0.5042807953110326 1101 48-49 -0.0275322103894311 1101 50-51 -0.31128955699946914 1101 52-53 -0.35464343264752785 1101 54-55 -0.19858316984183944 1101 56-57 -0.24808514989542374 1101 58-59 -0.4812494146723765 1101 60-61 -1.1927744786447008 1101 62-63 -0.7874796968607782 1101 64-65 -0.7810297332384337 1101 66-67 -0.3069954539107087 1101 68-69 -1.5279472923740798 1101 70-71 -0.22459820542932363 1101 72-73 -0.24622229639357585 1101 74-75 -1.0068481932276896 1101 76 -1.0166432194868236 1102 1 0.24716971214025563 1102 2 0.2604854672015051 1102 3 0.13082816672390862 1102 4 0.31081936196929405 1102 5 0.1992515902340557 1102 6 0.42206379200599997 1102 7 0.3806926693711006 1102 8 0.16172363592358607 1102 9 0.43728516663930606 1102 10-11 0.41832397202040283 1102 12-13 0.1910081069514291 1102 14-15 0.1774838045799001 1102 16-17 -0.37797353579247783 1102 18-19 -0.10544050305718145 1102 20-21 0.1440842185550295 1102 22-23 -2.771324791055463E-4 1102 24-25 -0.10469165451964102 1102 26-27 -0.17344249705289627 1102 28-29 0.44201463296821686 1102 30-31 0.407570454587443 1102 32-33 0.40759694232047394 1102 34-35 0.2447540935799566 1102 36-37 0.5174089236013302 1102 38-39 0.35115423284128866 1102 40-41 0.47004395769096163 1102 42-43 0.2942437090792609 1102 44-45 0.3849657697745883 1102 46-47 0.3409365959933126 1102 48-49 0.27617793453810435 1102 50-51 -0.27207216569512127 1102 52-53 0.34738555285971984 1102 54-55 0.13759074320163478 1102 56-57 0.5471612269161668 1102 58-59 0.4910984114145762 1102 60-61 0.35203711555819694 1102 62-63 0.647071027776903 1102 64-65 0.6602746145876495 1102 66-67 1.21877266203132 1102 68-69 1.0049512583505589 1102 70-71 0.44001049022285343 1102 72-73 0.15734292099772773 1102 74-75 0.3826300676418768 1102 76 1.4088060558754947 1103 1 -0.19764634137144554 1103 2 0.439212537232919 1103 3 0.2181394062090618 1103 4 0.03489962952782122 1103 5 0.2215988082334448 1103 6 0.4607787002861272 1103 7 0.5283565996437289 1103 8 0.5049890661455407 1103 9 0.5537846092257084 1103 10-11 0.16933745129475142 1103 12-13 0.2820945567335329 1103 14-15 0.15419911825173216 1103 16-17 0.14272677838610548 1103 18-19 0.2684320012501047 1103 20-21 0.25055021632537233 1103 22-23 -0.02688096343076296 1103 24-25 -0.28529366120859834 1103 26-27 0.508350347783427 1103 28-29 0.2674174909797742 1103 30-31 0.4555333612459975 1103 32-33 0.5999046346281673 1103 34-35 0.05226904239926 1103 36-37 0.27022132858765247 1103 38-39 -0.3384170654256593 1103 40-41 0.007390668950712609 1103 42-43 0.06595750253218569 1103 44-45 0.1288595571466331 1103 46-47 0.4540408830106486 1103 48-49 0.16849576163854607 1103 50-51 -0.174702144412052 1103 52-53 -0.014223852227949862 1103 54-55 0.15324899799033176 1103 56-57 -0.11393130374158744 1103 58-59 0.06437296294999584 1103 60-61 0.2059947521245249 1103 62-63 0.09467414929306983 1103 64-65 0.5854038331951301 1103 66-67 0.7532562943410355 1103 68-69 0.4121925677657785 1103 70-71 0.26754165471054137 1103 72-73 -0.1825020166732969 1103 74-75 -0.19406497644447285 1103 76 0.2688952420516344 1104 1 0.18060299549857461 1104 2 0.24794173906556694 1104 3 0.24172271944754442 1104 4 0.005721910694930443 1104 5 -0.01679038877643535 1104 6 0.08103430674862722 1104 7 0.03146728206475302 1104 8 0.1101494230300375 1104 9 0.4243416384034262 1104 10-11 0.3869646516805716 1104 12-13 0.39263229485747786 1104 14-15 0.20119694800818877 1104 16-17 0.2899175186802836 1104 18-19 0.28731312013122334 1104 20-21 0.3924600306489836 1104 22-23 0.24919812988971302 1104 24-25 0.04043578176222695 1104 26-27 0.3568673480245721 1104 28-29 0.3450880962365872 1104 30-31 0.3931026884704991 1104 32-33 0.5869072871480583 1104 34-35 0.6879574918808089 1104 36-37 0.38922301655485114 1104 38-39 0.012173723096161382 1104 40-41 -0.17563320372832436 1104 42-43 0.05836165010874339 1104 44-45 0.1844660196496477 1104 46-47 -0.02083251944895892 1104 48-49 0.3275022723691947 1104 50-51 -0.17694472941325756 1104 52-53 0.27087380873177835 1104 54-55 -0.20768661811770528 1104 56-57 -0.29801618437818433 1104 58-59 0.37985403360348613 1104 60-61 -0.028222754506771253 1104 62-63 0.3312789238288829 1104 64-65 0.005177163313284439 1104 66-67 0.3705217874685971 1104 68-69 0.1348113283155712 1104 70-71 0.03498800146723369 1104 72-73 0.4619156346409028 1104 74-75 0.09604835849645355 1104 76 0.20046022878903358 1105 1 -0.22158928584419613 1105 2 0.029033312484159524 1105 3 -0.16809687406695417 1105 4 0.19434945122930003 1105 5 0.06089858130805226 1105 6 0.4988465926586514 1105 7 0.22242412152581892 1105 8 0.47183017205949085 1105 9 0.2287718736169424 1105 10-11 0.03836908191578914 1105 12-13 0.18018653098445014 1105 14-15 0.08637621268048434 1105 16-17 0.37696935699953826 1105 18-19 0.576054841985524 1105 20-21 0.4446408938597557 1105 22-23 0.18918442813131975 1105 24-25 0.07843532503694206 1105 26-27 0.3370911434116479 1105 28-29 0.6253575641516349 1105 30-31 0.35480627376394125 1105 32-33 0.5532923065588804 1105 34-35 0.45425116623568584 1105 36-37 0.16771125587889912 1105 38-39 0.22014357922770245 1105 40-41 -0.5764096367039002 1105 42-43 -0.13871530810089183 1105 44-45 0.2695996117939714 1105 46-47 0.14326887356314444 1105 48-49 0.17328898166355344 1105 50-51 -0.04093194110542697 1105 52-53 0.2133035872200182 1105 54-55 0.18740358512503974 1105 56-57 0.3743519361972858 1105 58-59 0.8456909826786116 1105 60-61 0.4884705544678809 1105 62-63 0.8279905018147176 1105 64-65 0.472612650867525 1105 66-67 0.37255421496346486 1105 68-69 0.6348341645795585 1105 70-71 1.1193223243719999 1105 72-73 0.8755260479772886 1105 74-75 0.7050458465074101 1105 76 0.46960843614231607 1106 1 0.03478309969108295 1106 2 -0.046625365871172164 1106 3 -0.4929336933532511 1106 4 -0.2711324678711833 1106 5 0.04989281638356857 1106 6 0.030029593278023015 1106 7 -0.15439230612675914 1106 8 -0.25397858490844527 1106 9 -0.13298904877097328 1106 10-11 -0.12643152792746548 1106 12-13 -0.09960288251786409 1106 14-15 0.0417576029325204 1106 16-17 -0.16076469417011907 1106 18-19 -0.026355944616980764 1106 20-21 -0.08508896463754922 1106 22-23 -0.2503553307900219 1106 24-25 -0.04760688755060016 1106 26-27 -0.22956281818728996 1106 28-29 0.20609032893319323 1106 30-31 -0.3674530049058333 1106 32-33 0.09464730203270477 1106 34-35 -0.08333995292196761 1106 36-37 0.21259190764887137 1106 38-39 -0.738930221334499 1106 40-41 0.018735439288292355 1106 42-43 0.15734453276813554 1106 44-45 0.14593959940653178 1106 46-47 0.07428035576810288 1106 48-49 -0.01576242621676016 1106 50-51 -0.371462218869965 1106 52-53 -0.48313264128061917 1106 54-55 0.03937366469052961 1106 56-57 -0.37735133694578593 1106 58-59 0.06804554935639828 1106 60-61 0.11551329113947162 1106 62-63 0.6945634686068516 1106 64-65 0.6448695473371018 1106 66-67 0.5050045460892889 1106 68-69 0.5772325637410276 1106 70-71 0.7383370463692458 1106 72-73 -0.030567620134590356 1106 74-75 -0.015164739990275677 1106 76 -0.17825473027819072 1107 1 0.32837175817606123 1107 2 -0.060273271076418666 1107 3 -0.027952736941905698 1107 4 -0.16872027997443695 1107 5 -0.186617974983335 1107 6 0.3484278158764198 1107 7 0.2171163692858471 1107 8 0.45264435203611697 1107 9 0.13975745990443755 1107 10-11 0.259809479266778 1107 12-13 0.3732864530724882 1107 14-15 0.2894829520649793 1107 16-17 0.5897907438324239 1107 18-19 0.15937194366063778 1107 20-21 0.3551882253751444 1107 22-23 0.20208859642115584 1107 24-25 0.3264144835877829 1107 26-27 0.5184799240894762 1107 28-29 0.5419187250398281 1107 30-31 0.7234272320810504 1107 32-33 0.52524400114401 1107 34-35 0.4448286886353756 1107 36-37 0.2507848826806125 1107 38-39 0.5536798082888552 1107 40-41 0.4036010761905402 1107 42-43 0.2137876135975958 1107 44-45 0.41768102979163757 1107 46-47 0.4926309693948525 1107 48-49 0.2626442601988046 1107 50-51 0.03403397241230266 1107 52-53 0.49359186147011513 1107 54-55 0.48541683015815806 1107 56-57 0.47006190892810196 1107 58-59 0.44777999709233285 1107 60-61 0.4531666978258855 1107 62-63 0.4323438325509912 1107 64-65 0.9969114432321504 1107 66-67 1.052798663736347 1107 68-69 1.5263174135082664 1107 70-71 0.9515194416295074 1107 72-73 0.791454174194655 1107 74-75 0.596504747948785 1107 76 0.5426508981602325 1108 1 -0.06767286657039051 1108 2 -0.1003341230033925 1108 3 -0.28758762538004135 1108 4 -0.20979533068437917 1108 5 0.04183030087873618 1108 6 -0.3982760380789614 1108 7 -0.0478430627628299 1108 8 0.2239425264783108 1108 9 -0.45152043056209124 1108 10-11 -0.3975181069401188 1108 12-13 -0.15650563617700897 1108 14-15 -0.09794098302629806 1108 16-17 -0.004910067526608941 1108 18-19 0.08989932702777992 1108 20-21 0.09935658237311884 1108 22-23 -0.08011221493787701 1108 24-25 0.1619875059001572 1108 26-27 0.37928114112801126 1108 28-29 0.6442260272710669 1108 30-31 0.5241371712291141 1108 32-33 0.49380383887219637 1108 34-35 0.2388195608463306 1108 36-37 0.6262919820720896 1108 38-39 -0.2912745527659055 1108 40-41 -0.21873665200419623 1108 42-43 0.31698233976391776 1108 44-45 0.3413625713737858 1108 46-47 0.6679605839993172 1108 48-49 0.5499160654726438 1108 50-51 0.27046906369018586 1108 52-53 0.7889772570076445 1108 54-55 0.4423133818823004 1108 56-57 0.6562941604493986 1108 58-59 -0.01152527674134518 1108 60-61 -0.020464133817114316 1108 62-63 0.36489961348404876 1108 64-65 0.7172461288305314 1108 66-67 0.7377631667789402 1108 68-69 0.715845811074189 1108 70-71 0.8729190359499981 1108 72-73 0.8782949449857327 1108 74-75 0.2313931860826628 1108 76 0.4625291943062777 1109 1 0.16508575411926785 1109 2 0.046217601134536324 1109 3 0.12620547806823623 1109 4 -0.37358843413265674 1109 5 -0.03575590601781897 1109 6 -1.0362070725617158 1109 7 -0.43577409724559146 1109 8 0.12049425061623964 1109 9 0.048479569437908765 1109 10-11 -0.2940698310780476 1109 12-13 -0.005643567211485845 1109 14-15 -0.07638925888836923 1109 16-17 0.46922786350787504 1109 18-19 0.3097269132346696 1109 20-21 0.2933220996144996 1109 22-23 0.04488778506212299 1109 24-25 0.14474612658980845 1109 26-27 0.043074244576288834 1109 28-29 0.13991568244347974 1109 30-31 0.12758544709119235 1109 32-33 0.5067348733549579 1109 34-35 -0.4465252667398829 1109 36-37 0.040085085520374264 1109 38-39 -0.3947228286279767 1109 40-41 -0.4385642382110859 1109 42-43 0.06267199493633058 1109 44-45 -0.1284650148331039 1109 46-47 0.15502954951655568 1109 48-49 0.12750227236919187 1109 50-51 -0.3329792121718782 1109 52-53 0.04759794666281891 1109 54-55 -0.13096248018666756 1109 56-57 -0.46008514989542704 1109 58-59 0.04019886118969396 1109 60-61 0.6433289696311562 1109 62-63 0.3864513376219847 1109 64-65 -0.0629262849625789 1109 66-67 0.2291424771237729 1109 68-69 0.9356733972810929 1109 70-71 0.38585007043274544 1109 72-73 -0.8329119515659897 1109 74-75 -0.16946888288286033 1109 76 0.16080505637524567 1110 1 0.2208434257421743 1110 2 0.003272330650510469 1110 3 -0.1133547876704597 1110 4 0.06171684900559171 1110 5 0.09893248373226271 1110 6 -0.41013937246680854 1110 7 -0.21630241107033044 1110 8 0.6268213622897605 1110 9 -0.33541793135297837 1110 10-11 0.12391498385393618 1110 12-13 0.27314479090303223 1110 14-15 -0.3811187748643192 1110 16-17 0.029651779990004457 1110 18-19 0.17171678985599215 1110 20-21 -0.01832769475437601 1110 22-23 0.03243128395488526 1110 24-25 0.09155884408427539 1110 26-27 -0.3227909879450621 1110 28-29 -0.0487113343233645 1110 30-31 0.3169242639213081 1110 32-33 -0.07405443382631205 1110 34-35 -0.5988504802798928 1110 36-37 0.010229027310941774 1110 38-39 -0.49627297098798806 1110 40-41 0.14641678045389028 1110 42-43 -0.37694363302949796 1110 44-45 -0.1837086086325428 1110 46-47 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-0.4908046751820976 2116 14-15 0.04924669362718248 2116 16-17 -0.15315766503933048 2116 18-19 -0.50612950281959 2116 20-21 -0.3995128353770241 2116 22-23 0.16508281050022333 2116 24-25 -0.08794183270923384 2116 26-27 0.14878368493807415 2116 28-29 -0.22031608691774096 2116 30-31 -0.5420018903876098 2116 32-33 -0.40387846751558953 2116 34-35 -0.5570396816635679 2116 36-37 -0.548877605265389 2116 38-39 -0.006650471363990107 2116 40-41 -0.1716761658538246 2116 42-43 -0.03802048669170688 2116 44-45 -0.265901419581553 2116 46-47 0.6117847784594588 2116 48-49 0.401385822397458 2116 50-51 0.17500552496774446 2116 52-53 -0.3897253998606445 2116 54-55 -0.02277989115331991 2116 56-57 0.37598042387507036 2116 58-59 -0.3788231851641797 2116 60-61 0.02440584922415212 2116 62-63 -0.04656166407389151 2116 64-65 -0.9974231758613925 2116 66-67 0.33287339854830833 2116 68-69 0.9372986092652624 2116 70-71 1.6123198273575667 2116 72-73 1.537777703606423 2116 74-75 0.9498075444772311 2116 76 0.46309448543120624 2117 1 -0.5387203616907499 2117 2 0.289060802175527 2117 3 -0.49659868588752687 2117 4 0.23083576886018875 2117 5 -0.29746703158711085 2117 6 -0.20276530288052186 2117 7 0.079352773281407 2117 8 -1.1491869465210414 2117 9 -0.22705719048401818 2117 10-11 0.25391325285168875 2117 12-13 0.022495665059174996 2117 14-15 0.2077681906887321 2117 16-17 0.408395072356285 2117 18-19 0.23636972390481503 2117 20-21 -0.1355165470998827 2117 22-23 0.5596080192846316 2117 24-25 0.03763812398733535 2117 26-27 -0.44586524794159743 2117 28-29 -0.5778787222409747 2117 30-31 0.21704543407882682 2117 32-33 0.15287996118839686 2117 34-35 -0.7282428984900378 2117 36-37 0.18557955526662795 2117 38-39 0.3199811401423531 2117 40-41 0.04948065443692684 2117 42-43 0.12374876787062306 2117 44-45 0.773535635784981 2117 46-47 0.5021342990285902 2117 48-49 0.3851470348935919 2117 50-51 0.865540631679778 2117 52-53 0.33931883150341946 2117 54-55 0.07975645279967125 2117 56-57 -0.20536816876334996 2117 58-59 -0.3415513014648255 2117 60-61 0.3148481628647346 2117 62-63 -0.37412120629473833 2117 64-65 -0.5281995445591932 2117 66-67 -2.0044294161748653 2117 68-69 -2.2120982357703127 2117 70-71 -2.441654809202909 2117 72-73 -2.037693994506782 2117 74-75 -1.080659513982404 2117 76 -0.8341526534490882 2118 1 -0.8180476791640885 2118 2 0.028601409230489594 2118 3 0.21053831165144032 2118 4 0.006471535882340618 2118 5 -0.3007484097659443 2118 6 -0.1793854833563202 2118 7 -0.17003196831005596 2118 8 0.5530279837496721 2118 9 -0.6351786014766319 2118 10-11 -0.6396470424723546 2118 12-13 -0.3995716031935004 2118 14-15 -0.29715387657951453 2118 16-17 -0.1533358546330561 2118 18-19 -0.06920861899921249 2118 20-21 0.3977074069608264 2118 22-23 -0.5111466976964962 2118 24-25 -0.9633873066935479 2118 26-27 -0.5937323521253575 2118 28-29 -0.7101592800752599 2118 30-31 -1.375038241064729 2118 32-33 -0.08153349246213537 2118 34-35 -0.4255357614924975 2118 36-37 -0.7651997720508419 2118 38-39 -0.20319359324566477 2118 40-41 -0.6640140133235306 2118 42-43 -1.0716983199062398 2118 44-45 0.08424113209343176 2118 46-47 -0.21341123009364082 2118 48-49 -1.0535322103894273 2118 50-51 -1.565550426564684 2118 52-53 -1.0837738674301391 2118 54-55 0.09411248233207203 2118 56-57 -0.8731286281563015 2118 58-59 -1.5306407190202052 2118 60-61 -0.7095570873403574 2118 62-63 -1.1971318707738234 2118 64-65 0.5515789624137355 2118 66-67 0.14630889391537494 2118 68-69 -1.1979472923740815 2118 70-71 -0.4621634228206233 2118 72-73 -0.5817875137848745 2118 74-75 0.42610832851143954 2118 76 -1.8230780020955208 2119 1 -0.5428621012213526 2119 2 -0.5010910616072621 2119 3 -0.13304599375683068 2119 4 0.004880867801745126 2119 5 -0.9274716335199216 2119 6 -0.8023971482555794 2119 7 -0.6790457541000876 2119 8 -0.9867387182651726 2119 9 -0.09912345831650526 2119 10-11 -0.6075685695137096 2119 12-13 -1.081968209768256 2119 14-15 -1.737238712210484 2119 16-17 -0.9786274771144932 2119 18-19 -0.6004917579175526 2119 20-21 -0.3817199525301618 2119 22-23 -0.5172442586721004 2119 24-25 -0.938201728750812 2119 26-27 -1.9497768908316147 2119 28-29 -1.4734148474135438 2119 30-31 -1.3450806588802138 2119 32-33 -1.9216713503624518 2119 34-35 0.127486507860624 2119 36-37 -0.7545953181802219 2119 38-39 -1.3535117268617896 2119 40-41 -0.9203766856459055 2119 42-43 0.14330698232068784 2119 44-45 -0.8274767894336108 2119 46-47 -1.2730612831159078 2119 48-49 -0.5718248933162542 2119 50-51 0.4492958088541883 2119 52-53 0.02730778686466806 2119 54-55 -0.6365343893540398 2119 56-57 0.3813782647387214 2119 58-59 0.2355310731324991 2119 60-61 0.0605913750138356 2119 62-63 -1.0807479895437027 2119 64-65 -1.7493224161652634 2119 66-67 -2.2510930148863153 2119 68-69 -1.7406302192033465 2119 70-71 -2.9706469859171207 2119 72-73 -1.901246686637478 2119 74-75 -2.0449945346910994 2119 76 -2.412155414608776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 5.0 23 5.0 24 13.0 25 24.0 26 66.0 27 102.0 28 185.0 29 374.0 30 548.0 31 835.0 32 1389.0 33 2340.0 34 3474.0 35 4741.0 36 8203.0 37 16376.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.84784013649407 17.956208153452422 10.686865031150635 29.50908667890288 2 24.010547268826098 23.57366284931365 28.319933821058346 24.095856060801903 3 20.841196391179587 20.988547940955975 28.263061293074475 29.90719437478996 4 15.629604735930513 21.36338960266784 33.376418581805964 29.63058707959569 5 15.071219915725253 30.806814362898432 37.155856577824885 16.96610914355143 6 29.45221415091901 34.33032598298995 18.20437918465476 18.01308068143629 7 29.216968694258462 27.389292454049585 23.079905901817334 20.313832949874623 8 25.530594834940413 32.81286353178399 17.837292867667966 23.819248765607632 9 24.188920197502778 16.981619833001577 19.48142594938345 39.348034020112195 10-11 27.27166972571931 25.55386086911563 24.45518703306362 22.71928237210144 12-13 26.858051340382076 21.088074864927748 24.818395677687874 27.235478117002298 14-15 22.900240415686476 21.74340149419642 21.77442287309671 33.5819352170204 16-17 24.815810562779518 26.761109531318667 22.89636274332394 25.526717162577878 18-19 26.082516867874777 26.606002636817205 23.86578083395807 23.44569966134995 20-21 24.50042654395988 26.21306517074684 23.56590750458858 25.7206007807047 22-23 23.471550810433524 26.98084429852907 23.68611534782721 25.861489543210197 24-25 24.291032236382907 27.459090556575237 25.453041387689684 22.79683581935217 26-27 27.194116278468577 26.36687950779412 24.02476540082207 22.414238812915237 28-29 22.83044231316082 27.442287309670917 26.741721169505983 22.98554920766228 30-31 24.668459013003126 27.482356590750456 25.19711501176227 22.652069384484143 32-33 24.612879042473438 24.947651423105757 26.361709277977408 24.077760256443398 34-35 24.168239278235916 24.994183491456194 27.203164180647832 23.634413049660058 36-37 26.152314970400436 25.47501486441072 25.954553679911076 22.41811648527777 38-39 22.64948426957578 27.183775818835148 27.298813432257063 22.867926479332006 40-41 24.373755913450353 23.523253108600677 26.425044593232165 25.677946384716797 42-43 25.356099578626267 23.545226585321718 25.953261122456894 25.145412713595118 44-45 22.493084817620144 24.850709614042344 27.995501900059455 24.660703668278057 46-47 23.723599513998398 23.755913450352867 28.804642866375413 23.71584416927332 48-49 23.372023886461754 23.156166791613888 27.3944626838663 26.077346638058064 50-51 24.425458211617507 23.54393402786754 26.253134451826384 25.77747330868857 52-53 21.646459685133003 23.21950210686865 31.582348835405732 23.551689372592612 54-55 23.166507251247317 25.14282759868676 26.95757826435385 24.73308688571207 56-57 22.44008996199881 27.03513171160458 25.865367215572732 24.659411110823875 58-59 21.760204741100743 27.3518082878784 26.96533360907892 23.92265336194194 60-61 22.35090349766047 28.291497557066414 25.884755577385416 23.4728433678877 62-63 20.697722513765736 30.543132642245947 25.102758317607215 23.656386526381098 64-65 21.148825065274153 30.822325052348575 24.368585683633636 23.660264198743633 66-67 20.879973114804955 29.79603443373058 24.58573533593568 24.738257115528786 68-69 20.57492955561875 29.777938629372073 24.760230592249826 24.886901222759352 70-71 21.314272419409043 28.777499159837657 24.638730191557016 25.269498229196287 72-73 21.235426414704133 28.083395806943617 24.440968901067652 26.240208877284594 74-75 21.298761729958894 28.069177674947653 24.407362407259004 26.22469818783445 76 21.47196442881886 28.07434790476437 24.126877439702195 26.326810226714574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 1.5 15 0.5 16 3.0 17 6.0 18 6.5 19 5.5 20 8.5 21 13.0 22 14.5 23 22.5 24 40.5 25 52.0 26 66.0 27 112.0 28 172.5 29 201.0 30 227.0 31 302.0 32 421.0 33 491.0 34 586.0 35 774.0 36 917.0 37 967.0 38 1136.5 39 1686.5 40 2322.5 41 2619.0 42 2660.0 43 2556.0 44 2527.0 45 2483.0 46 2364.0 47 2307.0 48 2146.5 49 1931.5 50 1820.0 51 1640.0 52 1323.5 53 1091.5 54 996.0 55 923.0 56 741.5 57 617.5 58 602.0 59 552.5 60 450.5 61 417.5 62 437.0 63 430.5 64 451.5 65 520.5 66 575.5 67 589.0 68 605.5 69 603.5 70 585.0 71 585.0 72 521.5 73 419.0 74 336.0 75 292.0 76 232.5 77 133.5 78 67.0 79 40.0 80 27.0 81 11.0 82 5.5 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38683.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.67050125378073 #Duplication Level Percentage of deduplicated Percentage of total 1 74.04007377598927 45.66088462632164 2 15.006706908115358 18.509422743840965 3 5.059523809523809 9.360701083163146 4 2.4857478202548626 6.131892562624409 5 1.324614352783367 4.084481555205128 6 0.6748826291079812 2.4972210014735157 7 0.4610999329309189 1.9905384794354108 8 0.3101945003353454 1.5303880257477445 9 0.19701542588866533 1.0935036062352972 >10 0.414989939637827 3.551947884083447 >50 0.008383635144198525 0.38518212134529384 >100 0.008383635144198525 0.6049168885556964 >500 0.0041918175720992625 1.3313341778042034 >1k 0.0041918175720992625 3.2675852441641027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG 1264 3.2675852441641027 RNA PCR Primer, Index 23 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 515 1.3313341778042034 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 127 0.3283095933614249 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 107 0.2766072951942714 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 98 0.2533412610190523 RNA PCR Primer, Index 47 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 51 0.13184086032624148 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0025851149083576763 0.0 6 0.0 0.0 0.0 0.0025851149083576763 0.0 7 0.0 0.0 0.0 0.0025851149083576763 0.0 8 0.0 0.0 0.0 0.0025851149083576763 0.0 9 0.0 0.0 0.0 0.0025851149083576763 0.0 10 0.0 0.0 0.0 0.0025851149083576763 0.0 11 0.0 0.0 0.0 0.0025851149083576763 0.0 12 0.0 0.0 0.0 0.0025851149083576763 0.0 13 0.0 0.0 0.0 0.0025851149083576763 0.0 14 0.0 0.0 0.0 0.0025851149083576763 0.0 15 0.0 0.0 0.0 0.0025851149083576763 0.0 16 0.0 0.0 0.0 0.0025851149083576763 0.0 17 0.0 0.0 0.0 0.0025851149083576763 0.0 18 0.0 0.0 0.0 0.0025851149083576763 0.0 19 0.0 0.0 0.0 0.007755344725073029 0.0 20 0.0 0.0 0.0 0.007755344725073029 0.0 21 0.0 0.0 0.0 0.007755344725073029 0.0 22 0.0 0.0 0.0 0.012925574541788382 0.0 23 0.0 0.0 0.0 0.015510689450146059 0.0 24 0.0 0.0 0.0 0.018095804358503737 0.0 25 0.0 0.0 0.0 0.023266034175219087 0.0 26 0.0 0.0 0.0 0.025851149083576764 0.0 27 0.0 0.0 0.0 0.02843626399193444 0.0 28 0.0 0.0 0.0 0.033606493808649794 0.0 29 0.0 0.0 0.0 0.046532068350438174 0.0 30 0.0 0.0 0.0 0.05945764289222656 0.0 31 0.0 0.0 0.0 0.05945764289222656 0.0 32 0.0 0.0 0.0 0.06979810252565727 0.0 33 0.0 0.0 0.0 0.09047902179251868 0.0 34 0.0 0.0 0.0 0.11633017087609544 0.0 35 0.0 0.0 0.0 0.15769200940981826 0.0 36 0.0 0.0 0.0 0.20163896285189878 0.0 37 0.0 0.0 0.0 0.2947030995527751 0.0 38 0.0 0.0 0.0 0.4058630406121552 0.0 39 0.0 0.0 0.0 0.5713103947470465 0.0 40 0.0 0.0 0.0 0.798800506682522 0.0 41 0.0 0.0 0.0 1.1400356745857354 0.0 42 0.0 0.0 0.0 1.5458987151978905 0.0 43 0.0 0.0 0.0 1.8897189980094615 0.0 44 0.0 0.0 0.0 2.277486234263113 0.0 45 0.0 0.0 0.0 2.6109660574412534 0.0 46 0.0 0.0 0.0 2.9832226042447587 0.0 47 0.0 0.0 0.0 3.3994261044903444 0.0 48 0.0 0.0 0.0 3.7690975363854924 0.0 49 0.0 0.0 0.0 4.177545691906006 0.0 50 0.0 0.0 0.0 4.526536204534292 0.0 51 0.0 0.0 0.0 4.9685908538634544 0.0 52 0.0 0.0 0.0 5.446837111909624 0.0 53 0.0 0.0 0.0 6.0207326215650285 0.0 54 0.0 0.0 0.0 6.610138820670579 0.0 55 0.0 0.0 0.0 7.119406457617041 0.0 56 0.0 0.0 0.0 7.5537057622211305 0.0 57 0.0 0.0 0.0 8.037122250084016 0.0 58 0.0 0.0 0.0 8.463666209963034 0.0 59 0.0 0.0 0.0 8.838507871674896 0.0 60 0.0 0.0 0.0 9.187498384303183 0.0 61 0.0 0.0 0.0 9.554584701289972 0.0 62 0.0 0.0 0.0 10.022490499702712 0.0 63 0.0 0.0 0.0 10.60414135408319 0.0 64 0.0 0.0 0.0 11.128919680479797 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATAA 15 0.002208628 70.00001 1 AGGTATC 15 0.002208628 70.00001 23 GACAGCC 15 0.002208628 70.00001 9 ACTATAA 15 0.002208628 70.00001 33 GGCGTCG 15 0.002208628 70.00001 15 TATCTTT 15 0.002208628 70.00001 26 GTCCGTA 15 0.002208628 70.00001 13 CATAAGT 15 0.002208628 70.00001 3 CGTAAAG 15 0.002208628 70.00001 16 TCTTTAT 15 0.002208628 70.00001 1 CTTTATA 15 0.002208628 70.00001 2 TTCCTGT 15 0.002208628 70.00001 70 TAAAAGC 20 0.0069015697 52.5 37 TATTGTG 20 0.0069015697 52.5 44 CCGTAAA 20 0.0069015697 52.5 15 GTATCTT 20 0.0069015697 52.5 25 CTTTACT 20 0.0069015697 52.5 29 CAACTAT 20 0.0069015697 52.5 45 GTGGAGT 35 0.0012407752 40.0 8 AGAGACA 40 0.0023907197 35.0 6 >>END_MODULE