FastQCFastQC Report
Wed 25 May 2016
SRR1780205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476937
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC250.002363917735.16182733
TATCGCG406.9885765E-427.5250237
CATGGTT701.1199118E-625.16837914
CGTTACA450.001388737524.46925714
TTCGTTA551.5829797E-424.01931412
GTTAAGT500.002547415222.04549616
TCGTTAC703.1850337E-522.02233113
ACGGTTT602.8536704E-422.01770212
GACCATA500.002597622321.9715331
GCTCCTA1057.827839E-820.9252721
GATCCTA856.169392E-620.6790921
TGGAATT654.9168646E-420.31336223
GACGGTT654.930811E-420.30483411
TATACCG654.969337E-420.2814166
TCTATAC654.969337E-420.2814163
TTACATG550.0044264420.0413616
CCGTTTA550.004453462520.020313
TCTAAGC901.0111155E-519.5302523
AAAAAGC1151.971639E-719.16999815
CGATACG708.063985E-418.874340
Produced by FastQC (version 0.11.5)