FastQCFastQC Report
Wed 25 May 2016
SRR1780205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476937
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT152813.20398710940858No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG42310.8871192631311892Illumina PCR Primer Index 2 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC40090.8405722349073358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG40090.8405722349073358No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC10190.2136550529734535No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT5230.10965808901385297Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4850.037.6477844
CGTCTTC4900.036.365742
CCGTCTT4950.035.9983741
GTCTTCT5000.035.63838643
TATTGGA250.002351891235.19840626
TCTTTAT1700.033.687921
CTTTATA1800.031.7763392
GCGCTAT353.21384E-431.43044519
TATCGAT353.215817E-431.4271542
ATGCCGT23300.029.92997644
AAATCGT454.065696E-529.33200532
CTATAAC602.9402145E-729.3320054
TCTCGTT300.005742841429.33200534
AATCGTA454.065696E-529.33200533
GCTATAC407.027707E-427.4987553
TCGATTA407.027707E-427.49875544
GTTCGCA407.027707E-427.49875535
AAAATCG508.3336E-526.39880631
GCCGTCT6750.026.39880440
TATGCCG26850.025.97275543