Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780205_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 476937 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT | 15281 | 3.20398710940858 | No Hit |
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG | 4231 | 0.8871192631311892 | Illumina PCR Primer Index 2 (95% over 24bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC | 4009 | 0.8405722349073358 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG | 4009 | 0.8405722349073358 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC | 1019 | 0.2136550529734535 | No Hit |
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT | 523 | 0.10965808901385297 | Illumina PCR Primer Index 2 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTTCTG | 485 | 0.0 | 37.64778 | 44 |
CGTCTTC | 490 | 0.0 | 36.3657 | 42 |
CCGTCTT | 495 | 0.0 | 35.99837 | 41 |
GTCTTCT | 500 | 0.0 | 35.638386 | 43 |
TATTGGA | 25 | 0.0023518912 | 35.198406 | 26 |
TCTTTAT | 170 | 0.0 | 33.68792 | 1 |
CTTTATA | 180 | 0.0 | 31.776339 | 2 |
GCGCTAT | 35 | 3.21384E-4 | 31.430445 | 19 |
TATCGAT | 35 | 3.215817E-4 | 31.42715 | 42 |
ATGCCGT | 2330 | 0.0 | 29.929976 | 44 |
AAATCGT | 45 | 4.065696E-5 | 29.332005 | 32 |
CTATAAC | 60 | 2.9402145E-7 | 29.332005 | 4 |
TCTCGTT | 30 | 0.0057428414 | 29.332005 | 34 |
AATCGTA | 45 | 4.065696E-5 | 29.332005 | 33 |
GCTATAC | 40 | 7.027707E-4 | 27.498755 | 3 |
TCGATTA | 40 | 7.027707E-4 | 27.498755 | 44 |
GTTCGCA | 40 | 7.027707E-4 | 27.498755 | 35 |
AAAATCG | 50 | 8.3336E-5 | 26.398806 | 31 |
GCCGTCT | 675 | 0.0 | 26.398804 | 40 |
TATGCCG | 2685 | 0.0 | 25.972755 | 43 |