FastQCFastQC Report
Wed 25 May 2016
SRR1780204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70563
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG200.006925155452.5000045
ATTTCGG200.006925155452.50000443
TGGTTCA350.001247775340.00000413
CGGGGGC400.002404125635.00000415
CCTTATT450.004281367631.11111312
CCTCACT450.004281367631.11111368
TATCACA500.007165301628.00000241
TAGAGGG500.007165301628.00000239
GGGTGGA700.001215176125.0000021
GTAGAGG700.001215176125.00000238
ACCCATC750.001816111623.3333346
TACCCAT850.00375612720.5882365
TGTAGAT1000.00960316617.50000237
ATACCCA1000.00960316617.5000024
AGGCAAC2553.7524114E-715.09803970
GAGGCAA2758.814404E-714.069
GGCAACC2052.117516E-413.65853770
CGAGGCA3103.3881443E-612.41935468
ACGAGGC3752.2827535E-611.20000170
GACGAGG4258.712959E-710.70588269
Produced by FastQC (version 0.11.5)