FastQCFastQC Report
Wed 25 May 2016
SRR1780204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70563
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT34594.902002465881552No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG14862.1059195328996783Illumina PCR Primer Index 9 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC9321.3208055213072005No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG8851.2541983759193913No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2990.4237348185309581No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1890.2678457548573615Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT1350.1913183963266868No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1210.1714779700409563No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC760.10770517126539406No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT740.10487082465314684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT200.006925155452.50000417
AGGAGTG200.006925155452.5000045
GTCTAGG200.006925155452.5000042
TTGACTT200.006925155452.50000443
GGGGCCG200.006925155452.50000436
TCTTTAT350.001247775340.0000041
CTTTATA350.001247775340.0000042
GATTAAG350.001247775340.00000428
AACAAAT459.114372E-538.8888970
AGTGGGT400.002404125635.00000469
TTTATAC652.0763338E-532.3076933
TCCAATT450.004281367631.11111321
GTCTTCT9200.028.15217249
TTCTGCT9100.028.07692352
CCGTCTT9350.028.07486747
TCCATCC500.007165301628.0000028
GGTGTGA500.007165301628.00000229
GTGAGGA500.007165301628.00000232
TCTTCTG9300.027.84946350
CGTCTTC9300.027.84946348