##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780204_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70563 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.5404532120233 34.0 34.0 34.0 34.0 34.0 2 33.59685670960702 34.0 34.0 34.0 34.0 34.0 3 33.64881028300951 34.0 34.0 34.0 34.0 34.0 4 33.67372418973116 34.0 34.0 34.0 34.0 34.0 5 33.65063843657441 34.0 34.0 34.0 34.0 34.0 6 37.30513158454147 38.0 38.0 38.0 36.0 38.0 7 37.283363802559414 38.0 38.0 38.0 36.0 38.0 8 37.126992899961735 38.0 38.0 38.0 36.0 38.0 9 37.1676232586483 38.0 38.0 38.0 36.0 38.0 10-11 37.10402052066947 38.0 38.0 38.0 36.0 38.0 12-13 37.08534217649476 38.0 38.0 38.0 36.0 38.0 14-15 36.98430480563468 38.0 37.0 38.0 35.0 38.0 16-17 37.020577356404914 38.0 38.0 38.0 35.0 38.0 18-19 36.9673695846265 38.0 37.5 38.0 35.0 38.0 20-21 37.01142950271389 38.0 37.5 38.0 35.0 38.0 22-23 36.98387256777632 38.0 37.0 38.0 35.0 38.0 24-25 36.98320649632244 38.0 37.5 38.0 35.0 38.0 26-27 36.83149100803537 38.0 37.0 38.0 34.5 38.0 28-29 36.80481980641412 38.0 37.0 38.0 34.5 38.0 30-31 36.78137975993084 38.0 37.0 38.0 34.0 38.0 32-33 36.69276391309893 38.0 37.0 38.0 34.0 38.0 34-35 36.757330328925924 38.0 37.0 38.0 34.0 38.0 36-37 36.74359791958959 38.0 37.0 38.0 34.5 38.0 38-39 36.08994798973967 38.0 37.0 38.0 34.0 38.0 40-41 36.35523574677947 38.0 37.0 38.0 34.0 38.0 42-43 36.62425775548092 38.0 37.0 38.0 34.0 38.0 44-45 36.7236512053059 38.0 37.0 38.0 34.0 38.0 46-47 36.630450802828676 38.0 37.0 38.0 34.0 38.0 48-49 36.654018394909514 38.0 37.0 38.0 34.0 38.0 50-51 36.48568654960815 38.0 37.0 38.0 34.0 38.0 52-53 36.51941527429389 38.0 37.0 38.0 34.0 38.0 54-55 36.44873375565098 38.0 37.0 38.0 34.0 38.0 56-57 36.50724884146082 38.0 37.0 38.0 34.0 38.0 58-59 36.36244915890764 38.0 37.0 38.0 34.0 38.0 60-61 36.36507092952397 38.0 37.0 38.0 34.0 38.0 62-63 36.32919518727945 38.0 37.0 38.0 34.0 38.0 64-65 36.226818587645084 38.0 37.0 38.0 33.5 38.0 66-67 35.83132094723864 38.0 37.0 38.0 32.0 38.0 68-69 35.72503294927937 38.0 37.0 38.0 31.0 38.0 70-71 35.111892918384996 38.0 36.5 38.0 28.0 38.0 72-73 34.25288749061123 38.0 35.5 38.0 22.5 38.0 74-75 33.99919929708204 38.0 35.0 38.0 21.5 38.0 76 33.200912659609145 37.0 34.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3177964965453626 1101 2 0.17232028401430455 1101 3 0.213312976062916 1101 4 0.09837787515965601 1101 5 -0.11454484658931818 1101 6 -0.11569769349442538 1101 7 0.2783395651157221 1101 8 -0.08090983552335729 1101 9 0.00598590880479577 1101 10-11 0.23178945688415098 1101 12-13 0.239954534043342 1101 14-15 0.5547107776777551 1101 16-17 0.23997413436838855 1101 18-19 0.2826643648770215 1101 20-21 0.15435558552535156 1101 22-23 0.32536810386098125 1101 24-25 0.21504893370964595 1101 26-27 0.10224983045043956 1101 28-29 0.17216961285277677 1101 30-31 0.3853184078434282 1101 32-33 0.21267640949461963 1101 34-35 0.1433769862773957 1101 36-37 0.31993286642065044 1101 38-39 0.05294385931986767 1101 40-41 0.5601618607892007 1101 42-43 0.4506620525104523 1101 44-45 0.41392265079852564 1101 46-47 0.3989363980783125 1101 48-49 0.3934477553588067 1101 50-51 0.42631277642979626 1101 52-53 0.319989916848229 1101 54-55 0.6055484920726144 1101 56-57 0.10674697183148396 1101 58-59 0.24384101357568966 1101 60-61 0.27296846878746095 1101 62-63 0.32058384765121417 1101 64-65 0.09172806952562951 1101 66-67 0.12662866430247988 1101 68-69 -0.3964144783393593 1101 70-71 -0.8206010003773017 1101 72-73 -0.7825157135692677 1101 74-75 -0.8898178793503959 1101 76 -1.7149712036553737 1102 1 0.04682494655126135 1102 2 0.12380180921793738 1102 3 -0.11982360757537691 1102 4 0.12425439505105373 1102 5 0.059436973738854704 1102 6 0.3303181251810585 1102 7 0.008359633584611004 1102 8 0.1474221205621049 1102 9 -0.15855192616156444 1102 10-11 0.24942609954735673 1102 12-13 0.27133217540799137 1102 14-15 0.38281843911087776 1102 16-17 0.21808769829237207 1102 18-19 -0.006663931664121492 1102 20-21 0.13104074666335208 1102 22-23 0.07662533441227737 1102 24-25 0.20798955465168234 1102 26-27 0.33359205686998195 1102 28-29 0.37620691659495975 1102 30-31 0.3355840199317299 1102 32-33 0.7852652419819321 1102 34-35 0.35250223713325823 1102 36-37 0.5247847139002815 1102 38-39 0.24403109813464852 1102 40-41 0.634002021336947 1102 42-43 0.3834444866976767 1102 44-45 0.43310574606472585 1102 46-47 0.3872966861198677 1102 48-49 0.45263793361403515 1102 50-51 0.4971898865702755 1102 52-53 0.622398133115496 1102 54-55 0.6930706264133093 1102 56-57 0.7824169045430835 1102 58-59 0.8522697705111142 1102 60-61 0.7835103174476004 1102 62-63 0.7962301703994186 1102 64-65 0.7747170908926364 1102 66-67 0.9275258429118551 1102 68-69 1.1005280313997474 1102 70-71 0.9391676219813334 1102 72-73 0.4558150620180257 1102 74-75 0.413168781020282 1102 76 1.0700600795461384 1103 1 0.1456363160603047 1103 2 0.01723850172439967 1103 3 0.025292671494391072 1103 4 -0.01920813724868964 1103 5 0.18357134066392433 1103 6 0.15615791846271065 1103 7 0.30913482738306186 1103 8 0.19972186216417498 1103 9 0.33622843559554383 1103 10-11 0.22689379980575808 1103 12-13 0.26363191701005917 1103 14-15 0.4671078447956347 1103 16-17 0.25855281457143775 1103 18-19 0.2745763784133999 1103 20-21 0.29698389911812484 1103 22-23 0.20205169100142228 1103 24-25 0.19961746162842076 1103 26-27 0.25772642379505584 1103 28-29 0.29594851866213645 1103 30-31 0.38793544112036216 1103 32-33 0.5274874642041496 1103 34-35 0.2579285937224043 1103 36-37 0.3212188224274186 1103 38-39 0.05204143405196504 1103 40-41 0.4654748895540024 1103 42-43 0.2388966830801067 1103 44-45 0.5219429553670523 1103 46-47 0.43546206079687266 1103 48-49 0.2759971067406468 1103 50-51 0.3529004808855163 1103 52-53 0.34208805559611477 1103 54-55 0.34872954114198507 1103 56-57 0.1883600569978654 1103 58-59 0.24007338808216616 1103 60-61 -0.008892783327603127 1103 62-63 0.14191492492137314 1103 64-65 0.7652080469650002 1103 66-67 0.3795671865811059 1103 68-69 0.6726210546555578 1103 70-71 0.7099686555730642 1103 72-73 0.5631018837234549 1103 74-75 0.5780783417437902 1103 76 0.4440135679182333 1104 1 0.01802596598420081 1104 2 -0.03505982399858709 1104 3 0.21743879021585144 1104 4 0.20958942743928333 1104 5 0.08305383685874546 1104 6 0.10440753794520674 1104 7 0.13196587761137124 1104 8 0.03375534770450628 1104 9 -0.03010337566777821 1104 10-11 0.07715255170834467 1104 12-13 0.06679782264172474 1104 14-15 -0.26519048092735176 1104 16-17 -0.06710928588518783 1104 18-19 -0.019852845330888158 1104 20-21 -0.021768003469389896 1104 22-23 0.19483707912927883 1104 24-25 0.017334356605587686 1104 26-27 0.10332764145106665 1104 28-29 0.026329036165940067 1104 30-31 0.16760058571701109 1104 32-33 0.29622414609151804 1104 34-35 0.2314445754575658 1104 36-37 -0.013667022321442346 1104 38-39 0.2459531539910742 1104 40-41 -0.14575798715832633 1104 42-43 0.04732895096443457 1104 44-45 0.0855959234035808 1104 46-47 -0.16094585396721328 1104 48-49 -0.13319619615128886 1104 50-51 0.11688830432539987 1104 52-53 -0.02339139645868471 1104 54-55 -0.05757182872103073 1104 56-57 -0.3060082839458147 1104 58-59 -0.027628286194847362 1104 60-61 -0.04441789748285174 1104 62-63 -0.21650212226278853 1104 64-65 0.18268141073972544 1104 66-67 0.43567068734214587 1104 68-69 0.25618269849118036 1104 70-71 0.21712238464460398 1104 72-73 0.4588096462805282 1104 74-75 0.5297982808609945 1104 76 1.263009001708184 1105 1 -0.1619538715298816 1105 2 0.014871979357877763 1105 3 0.14182946803119023 1105 4 0.20976733172678053 1105 5 -0.04580816871558824 1105 6 0.3307610930658882 1105 7 0.06151577976401512 1105 8 0.04537842782074364 1105 9 0.41513175449885864 1105 10-11 0.3905013034132594 1105 12-13 0.24244865582679864 1105 14-15 0.15270669039448137 1105 16-17 0.12140995742857541 1105 18-19 0.16626469010171263 1105 20-21 0.13813830027253715 1105 22-23 0.16560147455120244 1105 24-25 0.05208499409595646 1105 26-27 0.3521564382250659 1105 28-29 0.36108560379921784 1105 30-31 0.3089455421304592 1105 32-33 0.12336048007716727 1105 34-35 0.04061257640638871 1105 36-37 0.422257783466371 1105 38-39 0.015389197399720445 1105 40-41 0.08824545232456416 1105 42-43 0.17592410010752957 1105 44-45 0.19752737095146955 1105 46-47 0.11344185877666746 1105 48-49 0.4020288527723963 1105 50-51 0.25630596429100194 1105 52-53 0.34442571793377397 1105 54-55 -0.004170891758441542 1105 56-57 0.3994711681089811 1105 58-59 0.35317584118462264 1105 60-61 0.4814968270620099 1105 62-63 0.4492453722518235 1105 64-65 0.607516849273793 1105 66-67 0.23330456031848001 1105 68-69 0.45703663907114134 1105 70-71 0.2876021332065477 1105 72-73 0.23773391835548807 1105 74-75 -0.43675571309025685 1105 76 -0.895207211302548 1106 1 -0.06513050710088208 1106 2 0.08809922159920092 1106 3 -0.0255993472849525 1106 4 0.061605634269305654 1106 5 0.16466680232276332 1106 6 0.04504680735160349 1106 7 0.11382966306381803 1106 8 0.37487053196073106 1106 9 0.395132973936704 1106 10-11 0.16075921451623998 1106 12-13 0.19925007037469555 1106 14-15 0.07158358814461252 1106 16-17 0.2015575094071167 1106 18-19 0.05880173052607773 1106 20-21 -0.004017665827099393 1106 22-23 0.12148831071973376 1106 24-25 0.1980212174969651 1106 26-27 0.19844629859943552 1106 28-29 0.17813944197982323 1106 30-31 0.373819635173561 1106 32-33 0.2772840213246468 1106 34-35 0.08375018996653694 1106 36-37 0.34150051256825975 1106 38-39 0.044686191485446614 1106 40-41 0.14754061725353296 1106 42-43 0.5538888583539787 1106 44-45 0.24203685208066616 1106 46-47 0.2113932032068888 1106 48-49 0.460378953376015 1106 50-51 0.41922285960226446 1106 52-53 0.3534495579435273 1106 54-55 0.5977436256490307 1106 56-57 0.47693595684137335 1106 58-59 0.39381360717450065 1106 60-61 0.39458139507615897 1106 62-63 0.38376156028913755 1106 64-65 0.3560687668397975 1106 66-67 0.24610865762962675 1106 68-69 0.6308370170968729 1106 70-71 0.5204537885934073 1106 72-73 0.5669516489816715 1106 74-75 0.1548545545763389 1106 76 0.9074877463219835 1107 1 -0.04604827175331394 1107 2 -0.24207537436367232 1107 3 0.13331110467411378 1107 4 0.19496492982760572 1107 5 0.13126207287078273 1107 6 0.10802683807357027 1107 7 0.30114711616954537 1107 8 0.38038750476530225 1107 9 0.32561911659912823 1107 10-11 -0.08520041422394087 1107 12-13 0.06558788219182787 1107 14-15 0.21366186425287026 1107 16-17 0.015419174018440174 1107 18-19 -0.09166018072637883 1107 20-21 -0.32164385305801346 1107 22-23 0.13858010882015748 1107 24-25 0.3514146966514673 1107 26-27 0.09067061222311423 1107 28-29 0.20887222577939468 1107 30-31 0.17823754045471674 1107 32-33 0.10646339866395493 1107 34-35 0.1877289009420693 1107 36-37 0.1839530774197371 1107 38-39 0.12403324152763417 1107 40-41 0.04467611843265473 1107 42-43 0.5525474767309007 1107 44-45 0.3142010198831784 1107 46-47 0.13599457487777045 1107 48-49 -0.1217806710371292 1107 50-51 0.23040176096743892 1107 52-53 0.02694212625663539 1107 54-55 0.4064561156427615 1107 56-57 -0.22188059840407703 1107 58-59 0.1041184469659413 1107 60-61 -0.11814009515556023 1107 62-63 0.36929843746617763 1107 64-65 0.33541798040073445 1107 66-67 0.4535251998939529 1107 68-69 0.6375980131809058 1107 70-71 0.7556931819427533 1107 72-73 0.27069020937629773 1107 74-75 0.23815002633160276 1107 76 -0.12520302194351984 1108 1 -0.09089484925134883 1108 2 0.0846639759791401 1108 3 0.03309754954317157 1108 4 0.15488400367271993 1108 5 -0.032905624105723064 1108 6 -0.049262135737826895 1108 7 -0.09249118887709784 1108 8 0.050995574901300245 1108 9 -0.20404256711448454 1108 10-11 -0.09901405927283946 1108 12-13 -0.04704558976501261 1108 14-15 0.06347640848126446 1108 16-17 -0.026325234209046755 1108 18-19 0.14319426459226747 1108 20-21 0.11720070128615134 1108 22-23 -0.1585987155026487 1108 24-25 0.10498331528695815 1108 26-27 -0.22216517512094214 1108 28-29 -0.08936313445439481 1108 30-31 -0.27065534478749953 1108 32-33 -0.22692987996929048 1108 34-35 -0.12662425180604941 1108 36-37 -0.1395941857026699 1108 38-39 -0.3501265876282602 1108 40-41 -0.10054137060860313 1108 42-43 0.007948173595018204 1108 44-45 -0.45708143487684794 1108 46-47 0.3807737139134062 1108 48-49 0.11724371920676901 1108 50-51 -0.23712661938547797 1108 52-53 0.12517748649042204 1108 54-55 -0.05094525560598129 1108 56-57 0.24174759087320297 1108 58-59 -0.0587341999937081 1108 60-61 0.09712347683500155 1108 62-63 -0.11488724310030562 1108 64-65 0.17539774886201087 1108 66-67 0.44601705107974965 1108 68-69 0.5311576400214122 1108 70-71 0.4443859997465083 1108 72-73 0.4526953796584081 1108 74-75 0.32485340949446595 1108 76 0.5815745435279851 1109 1 0.05026943975003206 1109 2 -0.012551854669730744 1109 3 0.18963229413589744 1109 4 0.13125307868422453 1109 5 0.18736588993017023 1109 6 -0.28356954484965513 1109 7 -0.00721737387479493 1109 8 -0.14187142924043883 1109 9 0.08627036431671797 1109 10-11 -0.26981479558208576 1109 12-13 0.04477447466205575 1109 14-15 0.20149987833860905 1109 16-17 0.1507226258921932 1109 18-19 0.19341285640082617 1109 20-21 -0.20457794574560495 1109 22-23 -0.24373447252059321 1109 24-25 0.08329670691144031 1109 26-27 0.09689833155186278 1109 28-29 0.04350617065374962 1109 30-31 -0.045471267475036825 1109 32-33 -0.04869702216902283 1109 34-35 0.019406581143790902 1109 36-37 -0.11346671216377757 1109 38-39 0.29501837325531 1109 40-41 0.2463868392395412 1109 42-43 0.24291135813252396 1109 44-45 0.09562220065006954 1109 46-47 0.16111195597506622 1109 48-49 -0.03687501065977017 1109 50-51 0.03888580583310386 1109 52-53 -0.015238988429040035 1109 54-55 0.1449245096954499 1109 56-57 -0.0282017878658678 1109 58-59 -0.024979022819294983 1109 60-61 0.18321413491139538 1109 62-63 -0.03293832455451451 1109 64-65 -0.27838734087567474 1109 66-67 -0.06758166037906221 1109 68-69 -0.35851102081613817 1109 70-71 -0.2979978014499949 1109 72-73 -0.8990993741381814 1109 74-75 -0.22259251779499323 1109 76 0.4123154547106864 1110 1 0.20178807757792327 1110 2 -0.07631163377695316 1110 3 0.03797559832366204 1110 4 -0.07926233779069491 1110 5 -0.018271477764258748 1110 6 -0.5517011601280757 1110 7 -0.22907655473076005 1110 8 0.06562972124276456 1110 9 -0.3796523232120421 1110 10-11 -0.2282823519557624 1110 12-13 -0.03241144342354829 1110 14-15 -0.5292065183480048 1110 16-17 -0.3141301122578355 1110 18-19 -0.11290329638335095 1110 20-21 0.18793240860322413 1110 22-23 -0.10250115452703312 1110 24-25 -0.06087034324962559 1110 26-27 -0.05387249219410961 1110 28-29 -0.25765581738122023 1110 30-31 -0.35114314966554616 1110 32-33 0.38526524198192647 1110 34-35 -0.3924779103426346 1110 36-37 -0.15422833204413422 1110 38-39 -0.5013460998233796 1110 40-41 -0.4298096632915289 1110 42-43 -0.128637192258644 1110 44-45 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-0.483252572828583 2116 2 0.10612739061328824 2116 3 -0.1947073285056078 2116 4 -0.35031924835980277 2116 5 0.16579356288614377 2116 6 -0.4682865259817319 2116 7 -0.010865172616938423 2116 8 0.11749963994195411 2116 9 -0.5326604532933459 2116 10-11 0.07911602202798207 2116 12-13 -0.35192363854549313 2116 14-15 -0.1906699329821464 2116 16-17 -0.49478051876189255 2116 18-19 -0.33875695491992985 2116 20-21 0.18142834356257254 2116 22-23 -0.5179483089985766 2116 24-25 -0.11038253837157441 2116 26-27 -0.056718020649391576 2116 28-29 0.25039296310658443 2116 30-31 0.14015766334257762 2116 32-33 -0.1614014246847404 2116 34-35 -0.08873807294425973 2116 36-37 -0.39878117757258735 2116 38-39 -0.23573634372581154 2116 40-41 -0.6478584437793344 2116 42-43 -1.1688810946976673 2116 44-45 -0.3480570446329452 2116 46-47 -0.4056490503142456 2116 48-49 -0.3917806710371252 2116 50-51 -0.5926550746700343 2116 52-53 -0.5745786110705566 2116 54-55 -0.5146037921913447 2116 56-57 -0.9871954985576821 2116 58-59 -0.6021488341400527 2116 60-61 -0.768892783327594 2116 62-63 -1.1491961861897337 2116 64-65 -0.7725697308127906 2116 66-67 -0.0782105911966724 2116 68-69 -0.17404561201110624 2116 70-71 -0.6378091222047146 2116 72-73 -0.42689811627654706 2116 74-75 0.18363389729934454 2116 76 -0.0026530987484321145 2117 1 -0.10134781092381928 2117 2 0.040413104899002406 2117 3 0.009578385780102394 2117 4 -0.33222401026456083 2117 5 -0.16896834187576104 2117 6 -0.05257224026744467 2117 7 0.07580149404972758 2117 8 -0.5615479791056686 2117 9 0.045434784801891226 2117 10-11 -0.10755064463868536 2117 12-13 -0.08406649568835434 2117 14-15 -0.013527075839292024 2117 16-17 -0.2928757568571356 2117 18-19 -0.14304266920564146 2117 20-21 -0.13880975167551668 2117 22-23 0.35586121481094324 2117 24-25 -0.19466825265728716 2117 26-27 0.2123295983982274 2117 28-29 -0.658178465464843 2117 30-31 -0.3507947176098014 2117 32-33 -0.2968776151609305 2117 34-35 0.3103095461033618 2117 36-37 -0.33401927281068566 2117 38-39 -0.11859348658295943 2117 40-41 -0.32928701520790327 2117 42-43 -0.44030966612624667 2117 44-45 0.006942955367051695 2117 46-47 -0.6432680979332872 2117 48-49 -0.5217806710371278 2117 50-51 -0.1957503127652771 2117 52-53 -0.3667214682134059 2117 54-55 -0.3296037921913424 2117 56-57 -0.505290736652924 2117 58-59 0.5957083087170929 2117 60-61 -0.15270230713711896 2117 62-63 0.3455657185721748 2117 64-65 -0.5070935403365908 2117 66-67 -0.19082963881572113 2117 68-69 -0.42023608820158387 2117 70-71 0.22861944922385646 2117 72-73 -0.15523144960987878 2117 74-75 0.44506246872791877 2117 76 -1.0945578606531967 2118 1 -0.08717114789219949 2118 2 -1.115857342211136 2118 3 -0.5020355727804144 2118 4 -0.16166784886871 2118 5 0.0013151913848687968 2118 6 -0.3569888160580703 2118 7 -0.2547328570953056 2118 8 -0.4575130827043594 2118 9 -0.538207527084694 2118 10-11 -0.0461002629592997 2118 12-13 -0.490854936255424 2118 14-15 -0.09662413145542814 2118 16-17 -0.4718288648178728 2118 18-19 -0.4405890159886354 2118 20-21 -0.5844749643254659 2118 22-23 -0.1535717186677914 2118 24-25 -0.541451240661651 2118 26-27 -0.5062091147969809 2118 28-29 -0.6803703956720426 2118 30-31 -0.7136082399653105 2118 32-33 -0.6300019335905915 2118 34-35 -0.7677711518246682 2118 36-37 -0.8046844854606263 2118 38-39 -0.27001115288612 2118 40-41 -0.07503401629841022 2118 42-43 -0.8318581939343161 2118 44-45 -0.6455379606634821 2118 46-47 -0.42669230731169705 2118 48-49 -0.5988799076783522 2118 50-51 -0.6439782298863221 2118 52-53 -0.6830518935133014 2118 54-55 0.05722826887735977 2118 56-57 -0.7315720888884769 2118 58-59 -1.1503931089492099 2118 60-61 -0.5970861675515167 2118 62-63 -0.6651758299556363 2118 64-65 -0.2041218936626592 2118 66-67 -0.4843683520109252 2118 68-69 -1.5128751285505402 2118 70-71 -0.7769694275482237 2118 72-73 -0.2485520602969018 2118 74-75 -0.10117526300599877 2118 76 -0.39079559238711425 2119 1 0.0879669393665381 2119 2 0.1253143824832037 2119 3 -0.012430905741382503 2119 4 -0.004465589823219318 2119 5 0.07603746532517164 2119 6 -0.21511579427441774 2119 7 -0.24045866855189502 2119 8 0.10302809522650591 2119 9 -0.9308718354071672 2119 10-11 -0.16324170154926065 2119 12-13 -0.6128992483015949 2119 14-15 -1.4625398516813348 2119 16-17 -0.4482764537212418 2119 18-19 -0.2917650850012308 2119 20-21 -0.2088155588764451 2119 22-23 -0.2398995285107759 2119 24-25 -0.6104638391845825 2119 26-27 -0.36281558162500716 2119 28-29 -0.2308265490885404 2119 30-31 -0.2608992472265257 2119 32-33 -0.8220518311888014 2119 34-35 -0.26727465831011443 2119 36-37 0.11975540779327076 2119 38-39 -0.18020788844126656 2119 40-41 -0.2997283624785183 2119 42-43 -0.12050711095783129 2119 44-45 -0.2830976950394515 2119 46-47 0.021424120417471215 2119 48-49 -0.02828473607777937 2119 50-51 -0.4411916600358836 2119 52-53 -0.09433470863152849 2119 54-55 -0.7907826539799672 2119 56-57 -0.40134184002110374 2119 58-59 0.007119458542874213 2119 60-61 -0.1817383117828868 2119 62-63 -0.3124482187100597 2119 64-65 -0.3140331454469276 2119 66-67 -0.9507309163999267 2119 68-69 -0.5013626851818387 2119 70-71 0.20292258511236128 2119 72-73 0.0364352170567912 2119 74-75 0.30371519811235004 2119 76 -1.3273685459029068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 8.0 23 15.0 24 35.0 25 59.0 26 98.0 27 174.0 28 335.0 29 511.0 30 824.0 31 1292.0 32 2035.0 33 3310.0 34 5211.0 35 7801.0 36 11689.0 37 37165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.0628091209274 18.298541728668 12.547652452418406 33.0909966979862 2 19.192778084831993 20.25282371781245 35.633405609171945 24.92099258818361 3 18.590479429729463 19.66611396907728 32.16558252908748 29.57782407210578 4 14.713093264175276 23.18920680810056 33.12359168402704 28.97410824369712 5 14.822215608746795 31.0261751909641 38.52018763374573 15.631421566543372 6 28.35196916230886 37.32834488329578 17.129373751116024 17.19031220327934 7 26.96313932230773 26.009381687286538 23.080084463529044 23.947394526876693 8 23.112679449569885 35.98769893570285 17.366041693238664 23.5335799214886 9 22.33465130450803 16.415118404829727 18.240437623116932 43.00979266754531 10-11 26.29281634851126 26.70521378059323 23.826226209203124 23.175743661692387 12-13 25.157660530306252 19.76531610050593 25.72736419936794 29.349659169819876 14-15 22.38566954352848 19.44290917336281 21.440414948344035 36.73100633476468 16-17 26.427447812593 25.808143077816986 20.340688462792116 27.423720646797893 18-19 28.519195612431442 24.58299675467313 21.08187010189476 25.815937531000664 20-21 24.154301829570738 23.170783555120956 21.06557260887434 31.609342006433966 22-23 24.133752816631944 25.532502869775946 20.7360798152006 29.597664498391506 24-25 26.350920454062326 25.654379774102576 23.203378541161797 24.7913212306733 26-27 26.766860819409604 28.86852883239091 21.810297181242294 22.554313166957186 28-29 26.47421453169508 24.67723877953035 22.30843359834474 26.540113090429827 30-31 25.510536683531026 22.414721596304013 28.285362016921052 23.78937970324391 32-33 21.87903008658929 28.71830846194181 25.878293156470107 23.524368294998794 34-35 22.431019089324433 27.60582741663478 23.119765316100505 26.84338817794028 36-37 27.297592222552897 26.01859331377634 22.562107620140868 24.121706843529893 38-39 22.84554228136559 24.896192055326445 27.056672760511884 25.201592902796083 40-41 22.7080764706716 21.005342743364086 28.007596048920824 28.278984737043494 42-43 26.06748579283761 20.651758003486247 27.37695392769582 25.903802275980333 44-45 21.74652438246673 24.74242875161203 29.869053186514176 23.641993679407054 46-47 24.097614897325794 22.5670677267123 28.118844153451526 25.216473222510384 48-49 24.1791023624279 21.243427858792852 25.002480053285716 29.574989725493527 50-51 23.045363717529018 21.482221560874677 27.45206411292037 28.020350608675937 52-53 19.289854456301462 21.685585930303418 34.08868670549722 24.935872907897906 54-55 20.341397049445177 22.899394866998286 29.827955160636595 26.931252922919946 56-57 22.549353060385755 24.00620721908082 25.8208976375721 27.62354208296133 58-59 20.330059662996188 23.979280926264472 30.05470288961637 25.635956521122967 60-61 22.94828734605955 26.93267009622607 26.1362186981846 23.98282385952978 62-63 19.769567620424304 32.96345110043507 22.715870923855277 24.551110355285346 64-65 19.10845627311764 34.75759250598755 21.80037696809943 24.333574252795373 66-67 19.34866714850559 33.9108314555787 21.82730326091578 24.913198134999927 68-69 19.05318651417882 34.34377790059946 21.922253872426058 24.680781712795657 70-71 19.550614344628205 32.97053696696569 22.253872426058983 25.22497626234712 72-73 19.85530660544478 30.67046469112708 22.807987188753312 26.666241514674834 74-75 19.13254821932174 30.37285829684112 23.847483808794976 26.647109675042163 76 19.483298612587333 30.70305967716792 23.072998596998424 26.74064311324632 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 3.0 17 6.0 18 7.0 19 8.0 20 9.5 21 11.0 22 17.5 23 39.5 24 47.5 25 40.0 26 93.5 27 145.5 28 189.5 29 235.0 30 311.5 31 455.0 32 641.0 33 760.0 34 869.0 35 1090.0 36 1279.0 37 1356.0 38 1692.5 39 3048.5 40 4833.5 41 5499.5 42 5400.0 43 4974.0 44 4392.5 45 3995.5 46 3754.0 47 3790.0 48 3526.5 49 3088.5 50 2950.0 51 2727.5 52 2199.5 53 1746.5 54 1599.0 55 1574.5 56 1432.5 57 1315.5 58 1316.0 59 1225.0 60 1157.5 61 1206.0 62 1231.0 63 1272.0 64 1291.5 65 1281.5 66 1332.5 67 1372.0 68 1342.0 69 1281.0 70 1191.0 71 1132.0 72 1025.0 73 773.5 74 530.5 75 432.0 76 326.0 77 164.5 78 78.0 79 47.0 80 29.0 81 8.5 82 3.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70563.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.204781542734864 #Duplication Level Percentage of deduplicated Percentage of total 1 68.54287811212104 34.4118022192934 2 16.366510472534294 16.433541657809332 3 5.916558459888218 8.911185748905234 4 3.232089425845424 6.490653742046115 5 1.9307852989329872 4.846732706942732 6 1.2420256308925648 3.7413375281663193 7 0.7085191667137131 2.4899734988591753 8 0.5222153220798283 2.0974164930629366 9 0.38389883136679276 1.7346201266952934 >10 1.120645853328064 7.81712795657781 >50 0.011291142099023316 0.388305485877868 >100 0.011291142099023316 1.0543769397559628 >500 0.005645571049511658 2.575003897226592 >1k 0.005645571049511658 7.007921998781232 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 3459 4.902002465881552 No Hit CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG 1486 2.1059195328996783 Illumina PCR Primer Index 9 (96% over 25bp) CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 932 1.3208055213072005 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 885 1.2541983759193913 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 299 0.4237348185309581 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 189 0.2678457548573615 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 135 0.1913183963266868 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 121 0.1714779700409563 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 76 0.10770517126539406 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 74 0.10487082465314684 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007085866530618029 0.0 2 0.0 0.0 0.0 0.011337386448988847 0.0 3 0.0 0.0 0.0 0.011337386448988847 0.0 4 0.0 0.0 0.0 0.06660714538780947 0.0 5 0.0 0.0 0.0 0.06802431869393308 0.0 6 0.0 0.0 0.0 0.06944149200005668 0.0 7 0.0 0.0 0.0 0.0722758386123039 0.0 8 0.0 0.0 0.0 0.0736930119184275 0.0 9 0.0 0.0 0.0 0.07511018522455111 0.0 10 0.0 0.0 0.0 0.07652735853067472 0.0 11 0.0 0.0 0.0 0.07652735853067472 0.0 12 0.0 0.0 0.0 0.07652735853067472 0.0 13 0.0 0.0 0.0 0.08077887844904554 0.0 14 0.0 0.0 0.0 0.08077887844904554 0.0 15 0.0 0.0 0.0 0.08077887844904554 0.0 16 0.0 0.0 0.0 0.08503039836741635 0.0 17 0.0 0.0 0.0 0.08644757167353996 0.0 18 0.0 0.0 0.0 0.09211626489803439 0.0 19 0.0 0.0 0.0 0.0992021314286524 0.0 20 0.0 0.0 0.0 0.10912234457151765 0.0 21 0.0 0.0 0.0 0.11053951787764126 0.0 22 0.0 0.0 0.0 0.11337386448988847 0.0 23 0.0 0.0 0.0 0.1218769043266301 0.0 24 0.0 0.0 0.0 0.12471125093887732 0.0 25 0.0 0.0 0.0 0.12612842424500093 0.0 26 0.0 0.0 0.0 0.13604863738786616 0.0 27 0.0 0.0 0.0 0.20265578277567564 0.0 28 0.0 0.0 0.0 0.25509119510224904 0.0 29 0.0 0.0 0.0 0.32453268710230576 0.0 30 0.0 0.0 0.0 0.3826367926533736 0.0 31 0.0 0.0 0.0 0.4634156711024191 0.0 32 0.0 0.0 0.0 0.5526975893882062 0.0 33 0.0 0.0 0.0 0.6476482008984878 0.0 34 0.0 0.0 0.0 0.823377690857815 0.0 35 0.0 0.0 0.0 1.1379901648172555 0.0 36 0.0 0.0 0.0 1.5673936765727081 0.0 37 0.0 0.0 0.0 2.0988336663690603 0.0 38 0.0 0.0 0.0 2.694046454940975 0.0 39 0.0 0.0 0.0 3.304848149880249 0.0 40 0.0 0.0 0.0 3.9780054702889616 0.0 41 0.0 0.0 0.0 4.673837563595652 0.0 42 0.0 0.0 0.0 5.359749443759477 0.0 43 0.0 0.0 0.0 6.065501750209033 0.0 44 0.0 0.0 0.0 6.742910590536117 0.0 45 0.0 0.0 0.0 7.430239644006066 0.0 46 0.0 0.0 0.0 8.157249550047474 0.0 47 0.0 0.0 0.0 8.840327083599053 0.0 48 0.0 0.0 0.0 9.462466164987317 0.0 49 0.0 0.0 0.0 10.060513300171477 0.0 50 0.0 0.0 0.0 10.71808171421283 0.0 51 0.0 0.0 0.0 11.398324901152161 0.0 52 0.0 0.0 0.0 12.121083287275201 0.0 53 0.0 0.0 0.0 12.771565834785935 0.0 54 0.0 0.0 0.0 13.389453396255828 0.0 55 0.0 0.0 0.0 13.984666184827743 0.0 56 0.0 0.0 0.0 14.59688505307314 0.0 57 0.0 0.0 0.0 15.211938267930785 0.0 58 0.0 0.0 0.0 15.783059110298598 0.0 59 0.0 0.0 0.0 16.36126581919703 0.0 60 0.0 0.0 0.0 16.93238666156484 0.0 61 0.0 0.0 0.0 17.472329691197938 0.0 62 0.0 0.0 0.0 18.04770205348412 0.0 63 0.0 0.0 0.0 18.650000708586653 0.0 64 0.0 0.0 0.0 19.18285787168913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 20 0.0069251554 52.500004 17 AGGAGTG 20 0.0069251554 52.500004 5 GTCTAGG 20 0.0069251554 52.500004 2 TTGACTT 20 0.0069251554 52.500004 43 GGGGCCG 20 0.0069251554 52.500004 36 TCTTTAT 35 0.0012477753 40.000004 1 CTTTATA 35 0.0012477753 40.000004 2 GATTAAG 35 0.0012477753 40.000004 28 AACAAAT 45 9.114372E-5 38.88889 70 AGTGGGT 40 0.0024041256 35.000004 69 TTTATAC 65 2.0763338E-5 32.307693 3 TCCAATT 45 0.0042813676 31.111113 21 GTCTTCT 920 0.0 28.152172 49 TTCTGCT 910 0.0 28.076923 52 CCGTCTT 935 0.0 28.074867 47 TCCATCC 50 0.0071653016 28.000002 8 GGTGTGA 50 0.0071653016 28.000002 29 GTGAGGA 50 0.0071653016 28.000002 32 TCTTCTG 930 0.0 27.849463 50 CGTCTTC 930 0.0 27.849463 48 >>END_MODULE