FastQCFastQC Report
Wed 25 May 2016
SRR1780203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213882
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGCA250.00235571635.1779342
CTTGTAC300.00575218629.3149413
CTACACG300.00575218629.31494131
CCATCTG406.9813E-427.52136440
CAGAACT407.0388516E-427.4827564
TCAGATG407.0388516E-427.4827564
TGACGCT2200.024.99601413
TAGCCCG450.001399562924.4291175
TGCCGAC2250.022.51163119
CTGCCGA2350.022.49080718
ACGCTGC2450.022.47168215
GACGCTG2500.022.01709214
ATCTGAC2000.021.99649210
ACAGAAC500.002582952121.9862063
TCTGACG2350.021.52848211
CATCTGA2050.021.4499579
CTATCCC654.9347576E-420.2949589
ACATCTG2300.020.0743628
ATCCCTA550.00447588619.9968111
GCTGCCG2900.019.74408517
Produced by FastQC (version 0.11.5)