FastQCFastQC Report
Wed 25 May 2016
SRR1780203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213882
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT51322.399453904489391No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG39001.8234353522035514RNA PCR Primer, Index 36 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG9600.4488456251577973No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC6120.28613908603809574No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT3730.1743952272748525RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC3230.15101785096455056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT207.8462035E-443.99532334
TCTTCTG4050.038.5728244
GTCTTCT4250.036.7576343
GCCTTTA301.2859076E-436.722871
CGTCTTC4300.036.33021542
GTATACC250.002330889235.253961
CTATGGC250.002347008335.204493
CGTTTTC250.002347008335.2044936
CATCGTT250.002349703635.19626232
AGCGTCA451.0308922E-634.226593
CCGTCTT4800.032.54581541
TTAATAC551.3632416E-732.004082
TCTTTAT701.0095391E-931.4767461
ACTTGAG353.2066787E-431.43257944
GCCGTCT5250.030.17527840
TCCGCTT300.005731005729.3370743
ACGTTTT300.005731005729.33707435
TTTCAAC300.005731005729.33707442
TCTATGG300.005731005729.3370742
CTGAGTC300.005737561329.3302149
Produced by FastQC (version 0.11.5)