FastQCFastQC Report
Wed 25 May 2016
SRR1780202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37858
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT26486.994558613767235No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG16034.234243752971631RNA PCR Primer, Index 32 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG6691.767129800834698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4201.1094088435733531No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3060.8082835860320143No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT1750.4622536848222304RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1320.3486713508373395No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1200.31697395530667233No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1190.3143325056791167No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG960.2535791642453379No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC870.2298061175973374No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG750.19810872206667018No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC590.15584552802578056No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT550.14527972951555815RNA PCR Primer, Index 42 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT460.12150668286755771RNA PCR Primer, Index 42 (95% over 24bp)
CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC420.1109408843573353No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC400.1056579851022241RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT380.1003750858471129No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC150.002208273270.03
TCTTTAT650.059.2307661
CTTTATA700.055.02
ATTTATG200.00690046952.518
AACTCGT305.807244E-446.66666437
TTTATAC950.044.2105263
ATGGCCT350.001240448740.018
CGAACTC450.004256530731.11111335
GAACTCG450.004256530731.11111336
ACTCGTA500.007123987727.99999838
TGCTTGA7300.027.32876655
TTGAAAA7300.027.32876658
TGAAAAA7350.027.14285559
CGTCTTC7250.027.03448348
TTCTGCT7250.027.03448352
GAAAAAA7400.026.9594660
GTCTTCT7300.026.84931449
CTTGAAA7450.026.77852657
CTGCTTG7200.026.73611354
TCTTCTG7200.026.73611350
Produced by FastQC (version 0.11.5)