##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37858 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.36282952084104 34.0 34.0 34.0 33.0 34.0 2 33.484441861693696 34.0 34.0 34.0 34.0 34.0 3 33.549500766020394 34.0 34.0 34.0 34.0 34.0 4 33.61572190818321 34.0 34.0 34.0 34.0 34.0 5 33.57554545934809 34.0 34.0 34.0 34.0 34.0 6 37.15941148502298 38.0 37.0 38.0 36.0 38.0 7 37.147419303713875 38.0 38.0 38.0 36.0 38.0 8 36.98235511648793 38.0 37.0 38.0 35.0 38.0 9 37.02263722330815 38.0 37.0 38.0 35.0 38.0 10-11 36.95026150351313 38.0 37.0 38.0 35.0 38.0 12-13 36.892334513180835 38.0 37.0 38.0 35.0 38.0 14-15 36.82472660996355 38.0 37.0 38.0 34.0 38.0 16-17 36.83883195097469 38.0 37.0 38.0 34.0 38.0 18-19 36.747622695335195 38.0 37.0 38.0 34.0 38.0 20-21 36.789714195150296 38.0 37.0 38.0 34.0 38.0 22-23 36.76706376459401 38.0 37.0 38.0 34.0 38.0 24-25 36.76068466374346 38.0 37.0 38.0 34.0 38.0 26-27 36.588818743726556 38.0 37.0 38.0 34.0 38.0 28-29 36.502337682920384 38.0 37.0 38.0 34.0 38.0 30-31 36.437093877119764 38.0 37.0 38.0 34.0 38.0 32-33 36.19348618521845 38.0 37.0 38.0 34.0 38.0 34-35 36.3072666279254 38.0 37.0 38.0 34.0 38.0 36-37 36.40346822336098 38.0 37.0 38.0 34.0 38.0 38-39 35.239817211685775 38.0 36.0 38.0 27.0 38.0 40-41 35.75236409741666 38.0 36.5 38.0 32.0 38.0 42-43 36.24521897617412 38.0 37.0 38.0 34.0 38.0 44-45 36.409411485022986 38.0 37.0 38.0 34.0 38.0 46-47 36.29939510803529 38.0 37.0 38.0 34.0 38.0 48-49 36.31681546832902 38.0 37.0 38.0 34.0 38.0 50-51 35.99400390934545 38.0 37.0 38.0 32.5 38.0 52-53 36.03933118495431 38.0 37.0 38.0 33.0 38.0 54-55 35.94520312747636 38.0 37.0 38.0 32.5 38.0 56-57 36.054162924613024 38.0 37.0 38.0 33.0 38.0 58-59 35.85000528289926 38.0 37.0 38.0 31.5 38.0 60-61 35.816406043636746 38.0 37.0 38.0 31.5 38.0 62-63 35.805668550900734 38.0 36.5 38.0 31.5 38.0 64-65 35.57162290665117 38.0 36.5 38.0 30.0 38.0 66-67 34.89288921760262 38.0 35.5 38.0 26.0 38.0 68-69 34.74716044165038 38.0 36.0 38.0 25.0 38.0 70-71 33.834592424322466 37.5 34.5 38.0 22.5 38.0 72-73 32.51893919382957 37.0 31.5 38.0 11.0 38.0 74-75 32.155964393259026 37.0 30.5 38.0 11.0 38.0 76 31.290876432986423 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12316136537612721 1101 2 -0.1270587178367677 1101 3 -0.09367098602130142 1101 4 0.1371766467177693 1101 5 0.14256964744971867 1101 6 0.06336358950978394 1101 7 0.04186578980537803 1101 8 0.059013347554405016 1101 9 0.20763333338293677 1101 10-11 0.13676081412469188 1101 12-13 0.1818485664226941 1101 14-15 0.34455957892798494 1101 16-17 0.38261610701614757 1101 18-19 0.524290155195537 1101 20-21 0.20299549674842865 1101 22-23 0.2629050517232656 1101 24-25 0.08994895271865033 1101 26-27 0.06555613899600132 1101 28-29 -0.3426304597752008 1101 30-31 -0.43746920176661064 1101 32-33 0.08756621541910903 1101 34-35 0.3840307778259131 1101 36-37 0.21877130808109513 1101 38-39 0.658627262624357 1101 40-41 -0.2422451331384181 1101 42-43 0.32656006922212555 1101 44-45 -0.040593414923051796 1101 46-47 0.13173874134226793 1101 48-49 -0.04961081278111834 1101 50-51 -0.0559653645750231 1101 52-53 0.2989140186162871 1101 54-55 0.6377801802765575 1101 56-57 -0.19223131559748907 1101 58-59 -0.1709775214817597 1101 60-61 -0.05579286206669565 1101 62-63 -0.04893671778403785 1101 64-65 -0.10562259480701641 1101 66-67 0.17845869725656627 1101 68-69 -0.17419818846045843 1101 70-71 0.17702398700016175 1101 72-73 -0.3888048315562358 1101 74-75 -0.12897357633247708 1101 76 -0.4532115236017411 1102 1 0.042925591145618114 1102 2 -0.21291379030053292 1102 3 -0.3192941744270996 1102 4 -0.1163305996590438 1102 5 -0.2475752800865152 1102 6 0.08150851704601791 1102 7 -0.13564145657143456 1102 8 -0.16562433360501672 1102 9 -0.24674347821126474 1102 10-11 0.24305066919716012 1102 12-13 0.3870949432342883 1102 14-15 0.09389291226131036 1102 16-17 0.26186248382774124 1102 18-19 0.41510174939843125 1102 20-21 0.2805896996469741 1102 22-23 0.32435432708558665 1102 24-25 0.3593112715592284 1102 26-27 0.2920199071119356 1102 28-29 0.6351956271813108 1102 30-31 0.6178641315667193 1102 32-33 0.09446476614375143 1102 34-35 0.12348005318823141 1102 36-37 0.3092060906897913 1102 38-39 0.44523595827653395 1102 40-41 0.5629432726586856 1102 42-43 0.49627021414966066 1102 44-45 0.5036964401494046 1102 46-47 0.6496807703277767 1102 48-49 0.4589399118565609 1102 50-51 0.4086143455699016 1102 52-53 0.4718995258626606 1102 54-55 0.7061859773780128 1102 56-57 0.3674498438227971 1102 58-59 0.4920949422863501 1102 60-61 0.27446800749852684 1102 62-63 0.8704835720710378 1102 64-65 -0.10721679770556136 1102 66-67 0.1428355088507729 1102 68-69 -0.21880688411263094 1102 70-71 -0.20219340430418242 1102 72-73 -0.6080222228605763 1102 74-75 -0.5214373444484188 1102 76 -0.12550137867420474 1103 1 0.052605057981537584 1103 2 -0.24057696601195033 1103 3 -0.1058169714227617 1103 4 0.13560000438199893 1103 5 0.24359154525993887 1103 6 -0.1505342207091971 1103 7 0.34621615476888223 1103 8 -0.06507424368646753 1103 9 0.05802749396688256 1103 10-11 0.01436665354074762 1103 12-13 0.19919163211612556 1103 14-15 0.578077827103165 1103 16-17 0.38600296833001835 1103 18-19 -0.08762225356358755 1103 20-21 -0.014581145587335698 1103 22-23 0.4053868035480903 1103 24-25 0.32664968264565886 1103 26-27 0.3418189127186224 1103 28-29 0.1906542117576393 1103 30-31 0.22431182013119866 1103 32-33 0.6579895730833485 1103 34-35 -0.29731228786751984 1103 36-37 0.003588826329270489 1103 38-39 -0.3775187227770971 1103 40-41 -0.08761739591214024 1103 42-43 -0.05241803296765113 1103 44-45 0.29143578215723664 1103 46-47 0.37132998221817815 1103 48-49 0.6607541507225321 1103 50-51 0.2541660222862987 1103 52-53 0.37722058795935 1103 54-55 0.5250794503495513 1103 56-57 0.04546211505944342 1103 58-59 0.596876493116774 1103 60-61 0.28890786786031697 1103 62-63 0.6125231362305641 1103 64-65 0.8521876241710871 1103 66-67 0.5406192811981754 1103 68-69 0.22621057066363193 1103 70-71 0.34295099429942866 1103 72-73 -1.15411870016937 1103 74-75 -1.1789005836317443 1103 76 -0.36579546520757944 1104 1 0.2692861870714296 1104 2 0.2622559674779197 1104 3 -0.08315350350382289 1104 4 -0.022431744890624827 1104 5 0.09893328381335209 1104 6 0.24199296013915728 1104 7 -0.19908525914566866 1104 8 -0.2696579811169215 1104 9 0.2373955711451785 1104 10-11 0.18814542950930502 1104 12-13 -0.26175982518569185 1104 14-15 -0.46671314834474487 1104 16-17 0.00986086226090066 1104 18-19 0.13794050484587927 1104 20-21 0.01969479744772684 1104 22-23 0.12500295382117343 1104 24-25 -0.2184985997289033 1104 26-27 -0.18429001485015561 1104 28-29 -0.14444864159338522 1104 30-31 -0.13330136959877592 1104 32-33 -0.29724497339207545 1104 34-35 -0.17552166972654248 1104 36-37 0.11933074864053594 1104 38-39 -0.9282818282847316 1104 40-41 -0.9466087695020562 1104 42-43 -0.06071265805060477 1104 44-45 0.13585413752448972 1104 46-47 -0.43654097893745103 1104 48-49 0.027172404002094197 1104 50-51 -0.3235737561834071 1104 52-53 -0.06757549187322098 1104 54-55 -0.6955065330101533 1104 56-57 -0.9293222246883985 1104 58-59 -0.19864185714609306 1104 60-61 -0.15484181311563816 1104 62-63 -0.13159406044137967 1104 64-65 -0.36730091648533403 1104 66-67 -0.6227720719741967 1104 68-69 -0.9799044821667522 1104 70-71 -1.5408221668459916 1104 72-73 -1.3872104259618254 1104 74-75 -0.7706518980107973 1104 76 -0.3031136215038366 1105 1 0.10029728124041526 1105 2 0.4115277483286448 1105 3 0.08094555533207881 1105 4 0.008274391078671783 1105 5 0.2219681436903187 1105 6 0.5029425368782015 1105 7 0.270918421384323 1105 8 0.1191637234942533 1105 9 0.29286641608970854 1105 10-11 0.42238487427506755 1105 12-13 0.47195382958058474 1105 14-15 0.30191296238663057 1105 16-17 0.1982251295725348 1105 18-19 0.11159842587222357 1105 20-21 0.3129804591544385 1105 22-23 0.6063636983398126 1105 24-25 0.18357301286902583 1105 26-27 0.3154208006501307 1105 28-29 0.6802266830819335 1105 30-31 0.5909217756770033 1105 32-33 0.8990850124115966 1105 34-35 0.39609092820185055 1105 36-37 0.16373371409613213 1105 38-39 -1.0253426621876685 1105 40-41 0.04689772400444525 1105 42-43 0.42971796395897144 1105 44-45 -0.021856572817796405 1105 46-47 0.04689663607911143 1105 48-49 0.028434300000832025 1105 50-51 -0.02970972547727513 1105 52-53 0.4203576276388432 1105 54-55 0.1869531126073838 1105 56-57 0.5098438723724357 1105 58-59 0.4528119522024525 1105 60-61 0.68342518304609 1105 62-63 0.3651986205618343 1105 64-65 0.8152796608320827 1105 66-67 1.1765338852264975 1105 68-69 1.2686890295846567 1105 70-71 0.07543000203775563 1105 72-73 0.022984642127980237 1105 74-75 0.062304619156243746 1105 76 -0.35558746345136427 1106 1 0.05518449928553082 1106 2 0.23107662050909994 1106 3 -0.0844679785025022 1106 4 0.09098115799596229 1106 5 0.24452453466776092 1106 6 -0.10608001951277402 1106 7 0.2107680454444747 1106 8 0.3146524452987691 1106 9 0.1048964912776782 1106 10-11 0.35095630284649815 1106 12-13 -0.23105368921640235 1106 14-15 0.1214618345670857 1106 16-17 0.14935294912140762 1106 18-19 0.5552074484286109 1106 20-21 0.5874165493800021 1106 22-23 0.30185242014432134 1106 24-25 0.36026474219233506 1106 26-27 0.19136065027419136 1106 28-29 0.19150487857066167 1106 30-31 0.3653578659025669 1106 32-33 0.8088594485018135 1106 34-35 0.1366924319612437 1106 36-37 -0.03551440620461932 1106 38-39 0.1927024505942896 1106 40-41 0.38524358866609276 1106 42-43 0.042499918846189644 1106 44-45 0.12851936703182787 1106 46-47 -0.06588531880810677 1106 48-49 -0.12946043684126352 1106 50-51 0.25600456023700957 1106 52-53 -0.04580778589498635 1106 54-55 -0.22282132348284023 1106 56-57 0.8369115415453692 1106 58-59 0.6670976664881678 1106 60-61 -0.019582335750897073 1106 62-63 -0.14607957492689394 1106 64-65 0.25136988639599167 1106 66-67 0.4848045619182244 1106 68-69 1.2085386536448013 1106 70-71 0.5340766185791068 1106 72-73 -0.6762634781727712 1106 74-75 -0.14446229813698608 1106 76 0.7797508824132962 1107 1 0.1348714215091249 1107 2 0.42940029855667206 1107 3 -0.19681852700490765 1107 4 0.25442254835711253 1107 5 0.23306145072840678 1107 6 0.5844783436081471 1107 7 0.40724283898570235 1107 8 0.5016362983740805 1107 9 0.7537316940386773 1107 10-11 0.4989903223214185 1107 12-13 0.5560780746194212 1107 14-15 0.6335103986001087 1107 16-17 0.676747254557128 1107 18-19 0.38255245027750107 1107 20-21 0.7353889393713828 1107 22-23 0.6737247238544128 1107 24-25 0.6044407559973379 1107 26-27 0.5392282701435391 1107 28-29 0.6480252779297118 1107 30-31 0.23944883102027603 1107 32-33 1.280910477714194 1107 34-35 0.9072766794652551 1107 36-37 0.9164762261138861 1107 38-39 0.5478731642637058 1107 40-41 0.29631224391076216 1107 42-43 0.5641010528286898 1107 44-45 0.5834065850769434 1107 46-47 0.77964038068653 1107 48-49 1.1382580396779005 1107 50-51 1.088526438703667 1107 52-53 0.7748812317310367 1107 54-55 0.8087637868339357 1107 56-57 0.46337524177955913 1107 58-59 0.8132519867149597 1107 60-61 0.19784648219560097 1107 62-63 0.624997708445477 1107 64-65 0.4707708478159418 1107 66-67 1.248917713650009 1107 68-69 0.5965886967854388 1107 70-71 0.6499092329017984 1107 72-73 -0.2600179463103345 1107 74-75 0.2846329810445667 1107 76 -0.5904574252410839 1108 1 -0.22383933147781931 1108 2 -0.06712651444693307 1108 3 -0.014823528394181551 1108 4 -0.2070945397229096 1108 5 0.09816286778870165 1108 6 -0.4760940376088598 1108 7 0.03630646777148172 1108 8 0.414945550944239 1108 9 0.09285367236599029 1108 10-11 -0.058595118078699215 1108 12-13 -0.20065990815358248 1108 14-15 0.06140703655509583 1108 16-17 0.44529407311784297 1108 18-19 0.45778168061926294 1108 20-21 0.36730058149419165 1108 22-23 0.11697284833343957 1108 24-25 0.21106759678644238 1108 26-27 0.4187086813688836 1108 28-29 0.13482716734343825 1108 30-31 0.3269036795893214 1108 32-33 0.24749841880893797 1108 34-35 0.6364375574869285 1108 36-37 0.3228391046912691 1108 38-39 -0.273813415341742 1108 40-41 0.024500629573445565 1108 42-43 0.4504583743068764 1108 44-45 0.299508279992196 1108 46-47 0.296450605749051 1108 48-49 0.09005020416802978 1108 50-51 -0.05060943237162974 1108 52-53 -0.29013682884134795 1108 54-55 -0.0034062604014053477 1108 56-57 0.10265004033470859 1108 58-59 -0.10450294521057657 1108 60-61 -0.11904709935483027 1108 62-63 0.14326667204647237 1108 64-65 0.18742825265061214 1108 66-67 0.19730615488368386 1108 68-69 0.3646492691666623 1108 70-71 0.502854495474736 1108 72-73 0.28346635488444605 1108 74-75 0.15695862705734953 1108 76 0.13377152724571673 1109 1 0.024530942316182802 1109 2 0.05557376079570986 1109 3 0.16082474047442474 1109 4 0.027296304837427954 1109 5 -0.11862693374686017 1109 6 -0.30812358997739864 1109 7 0.1746521145917015 1109 8 0.03337232191337591 1109 9 -0.22258888883928307 1109 10-11 -0.9101451687812911 1109 12-13 -0.1906642540901231 1109 14-15 -0.7437139253455172 1109 16-17 0.11786397026401119 1109 18-19 0.46350383040920917 1109 20-21 0.013867291620222488 1109 22-23 -0.07624024742202806 1109 24-25 0.1801027988724968 1109 26-27 -0.12446095502109245 1109 28-29 -0.4968184939632394 1109 30-31 -0.16205039834780877 1109 32-33 0.2804551043079968 1109 34-35 -0.039935034139901404 1109 36-37 0.43090806021784545 1109 38-39 -0.6963470963500029 1109 40-41 0.08291726002397581 1109 42-43 -0.17959377693171774 1109 44-45 -0.061892560222204906 1109 46-47 -0.37010741250389145 1109 48-49 0.019790896620591525 1109 50-51 -0.06017049278014497 1109 52-53 -0.2152056395033739 1109 54-55 -0.4608522983559169 1109 56-57 -0.10142789679407116 1109 58-59 -0.08092623943047528 1109 60-61 -0.24652790480173792 1109 62-63 -0.48124441009172614 1109 64-65 0.04412099493657706 1109 66-67 0.5011595519574215 1109 68-69 0.8009812987190301 1109 70-71 1.5147333887095655 1109 72-73 -0.015027053778460697 1109 74-75 -0.30367443103332903 1109 76 0.28196796357774545 1110 1 -0.29185701755003635 1110 2 -0.24914567435850898 1110 3 0.12998118789174384 1110 4 0.05613316845689553 1110 5 0.09735225614537057 1110 6 -0.059071193098908736 1110 7 -0.028395079759839348 1110 8 0.10249160842396776 1110 9 -0.19167101444315193 1110 10-11 -0.10115222935356627 1110 12-13 -0.26145578140339865 1110 14-15 -0.3655273775937573 1110 16-17 0.1067900200596199 1110 18-19 0.17524667693466256 1110 20-21 0.25812593153103336 1110 22-23 -0.14231233958108191 1110 24-25 -0.34431191684657136 1110 26-27 -0.19566125230835496 1110 28-29 -0.32350002499259745 1110 30-31 0.1823568851899111 1110 32-33 -0.31278161066784804 1110 34-35 0.024204690869389367 1110 36-37 -0.2603591267015162 1110 38-39 -0.133024911288679 1110 40-41 -0.24067991574711556 1110 42-43 -0.31604862643004594 1110 44-45 0.5399283242073807 1110 46-47 0.5272170022118345 1110 48-49 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2116 14-15 0.15608196698768495 2116 16-17 -1.0737421019390752 2116 18-19 -0.4952919343567075 2116 20-21 -0.04966121966948123 2116 22-23 0.11842743978296966 2116 24-25 -0.4570958234007563 2116 26-27 -0.7244737117502709 2116 28-29 -0.2088991164916223 2116 30-31 -0.06994681370690614 2116 32-33 -1.3323143815958147 2116 34-35 -0.1608050430696082 2116 36-37 -0.5077958560980136 2116 38-39 0.46859741187809334 2116 40-41 0.12259068775710347 2116 42-43 -0.1319473934644435 2116 44-45 -0.3419665492514099 2116 46-47 -0.5222314079114625 2116 48-49 -1.0328943948706737 2116 50-51 -0.7603235735302434 2116 52-53 -0.9227576231747534 2116 54-55 -0.39266758091746823 2116 56-57 -0.13217161410495493 2116 58-59 -0.5570073722280284 2116 60-61 -0.9575242053502748 2116 62-63 -0.6172949267392553 2116 64-65 0.19318337534224383 2116 66-67 -0.5177204072210415 2116 68-69 -0.14142206905747656 2116 70-71 -0.5959312368804319 2116 72-73 0.7445086012795841 2116 74-75 1.4987876176973742 2116 76 1.675385491323631 2117 1 -0.8664946987094595 2117 2 -0.2897253845034342 2117 3 0.13432901397870012 2117 4 -0.13082335328223138 2117 5 -0.22076368588361106 2117 6 -0.07863641049021908 2117 7 -0.3834675435279564 2117 8 -1.1076533191122593 2117 9 -0.7003666666170645 2117 10-11 -0.0272391858753096 2117 12-13 -0.6701514335773027 2117 14-15 -0.014440421072016818 2117 16-17 -0.10371722631718683 2117 18-19 0.17995682186219852 2117 20-21 -0.19767116991824452 2117 22-23 -0.18542828161006497 2117 24-25 -0.34105104728134705 2117 26-27 -0.738777194337338 2117 28-29 -2.2709637931085425 2117 30-31 -0.6571358684332722 2117 32-33 -1.725100451247556 2117 34-35 -0.2613025555074202 2117 36-37 -0.6128953585855754 2117 38-39 0.4959605959576905 2117 40-41 -0.9185784664717502 2117 42-43 -0.39177326411120816 2117 44-45 -0.14992674825639085 2117 46-47 -0.02459459199106817 2117 48-49 -0.8312774794477846 2117 50-51 0.04303463542498065 2117 52-53 -0.23208598138371173 2117 54-55 -0.0988864863901 2117 56-57 0.5214353510691794 2117 58-59 -1.2463108548150927 2117 60-61 -1.0787928620666918 2117 62-63 0.2443966155492987 2117 64-65 -1.3289559281403456 2117 66-67 -1.3118746360767588 2117 68-69 -2.0945315217937903 2117 70-71 -0.9619760129998411 2117 72-73 -1.6678048315562322 2117 74-75 -0.5989735763324759 2117 76 -0.37187819026840785 2118 1 -0.32663962624568654 2118 2 -0.749145674358509 2118 3 -0.2395840294995608 2118 4 0.3256983858482059 2118 5 0.006047908319281703 2118 6 -0.2373320626641302 2118 7 -0.32404725367288023 2118 8 -0.24533447853254842 2118 9 -0.21775797096488958 2118 10-11 0.0075434228203477005 2118 12-13 -0.25058621618600085 2118 14-15 -0.40248389933288564 2118 16-17 -0.19103606689689911 2118 18-19 0.10350754649987692 2118 20-21 0.19725636631364551 2118 22-23 0.08160070389718044 2118 24-25 -0.7421380038030847 2118 26-27 -1.2804438610040023 2118 28-29 -0.5278478510795566 2118 30-31 -0.8024257235057419 2118 32-33 -0.4779990019721936 2118 34-35 -0.6192735700001748 2118 36-37 -0.5820982571362947 2118 38-39 0.21697508871131532 2118 40-41 -0.46241904618189267 2118 42-43 -0.06170080034308967 2118 44-45 -0.7100716757926193 2118 46-47 -0.28147864996207517 2118 48-49 -0.09178472582460273 2118 50-51 -1.105878408053286 2118 52-53 -0.25273815529676114 2118 54-55 -0.514828515375612 2118 56-57 -1.0745791416844455 2118 58-59 -0.5368905649600251 2118 60-61 -0.11683634032755918 2118 62-63 -0.8758932395231582 2118 64-65 -0.7847530295896235 2118 66-67 -0.8752804331782116 2118 68-69 -1.1318503623735054 2118 70-71 -0.017410795608533647 2118 72-73 1.1963256032263772 2118 74-75 -0.12288661981074256 2118 76 -0.8791245670800016 2119 1 0.35320227098750934 2119 2 0.3723958276177797 2119 3 0.1616017412514239 2119 4 -0.1990051714640515 2119 5 -0.19652126164118755 2119 6 0.4168181349643305 2119 7 -0.46831602837644226 2119 8 -0.6228048342637749 2119 9 -0.7049121211625149 2119 10-11 -0.6999664586025816 2119 12-13 -0.5065150699409458 2119 14-15 -1.1712586028901981 2119 16-17 -0.9158384384383993 2119 18-19 -0.38216439025901394 2119 20-21 -0.1219135941606666 2119 22-23 0.15472323354145345 2119 24-25 0.23167622544592348 2119 26-27 0.026374320814177565 2119 28-29 -0.06490318704793197 2119 30-31 0.14816716186975043 2119 32-33 -0.7107065118536156 2119 34-35 -0.932514676719542 2119 36-37 -0.19622869191890402 2119 38-39 1.1739908989880021 2119 40-41 0.6556639577706775 2119 42-43 0.04156006922212896 2119 44-45 0.5152247668951304 2119 46-47 -0.4776248950213713 2119 48-49 -0.2464289945992988 2119 50-51 0.17030736269770586 2119 52-53 0.32927765497991857 2119 54-55 -0.10721981972343997 2119 56-57 0.37749595712978135 2119 58-59 0.5915679330636934 2119 60-61 0.8075707742969485 2119 62-63 0.46106328221596726 2119 64-65 0.5339228597384391 2119 66-67 1.0441859699838432 2119 68-69 0.25698362972136124 2119 70-71 1.3743876233637948 2119 72-73 2.9231042593528613 2119 74-75 2.6192082418493428 2119 76 -0.5233933417835566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 7.0 23 8.0 24 23.0 25 32.0 26 85.0 27 162.0 28 271.0 29 453.0 30 683.0 31 1029.0 32 1537.0 33 2639.0 34 3903.0 35 5057.0 36 6527.0 37 15440.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.01547889481748 16.406043636747846 13.304981773997572 37.27349569443711 2 18.072798351735432 20.603307094933697 36.628981985313544 24.694912568017326 3 17.584130170637646 17.745258597918536 34.16715093243172 30.503460299012097 4 14.160811453325586 27.251835807491155 31.034391674150775 27.552961065032488 5 14.876644302393153 29.25933752443341 40.855301389402506 15.008716783770934 6 27.449944529557825 37.315758888477994 16.089069681441178 19.145226900523006 7 25.909979396692904 24.589254582915103 22.904009720534628 26.59675629985736 8 22.185535421839507 36.6791695282371 15.28078609540916 25.854508954514237 9 21.10254107454171 16.56717206402874 16.07322098367584 46.25706587775371 10-11 26.38279888002536 27.821068202229384 23.220983675841303 22.575149241903958 12-13 25.19282582281156 17.922235722964764 25.76866184161868 31.116276612605 14-15 21.373289661366158 17.353003328226528 19.81615510592213 41.45755190448518 16-17 27.740503988588937 25.87696127634846 18.166569813513654 28.21596492154895 18-19 31.31438533467167 24.107190025886208 18.579956680226108 25.998467959216015 20-21 25.04358391885467 21.369327486924824 18.490147392889217 35.09694120133129 22-23 22.229119340694172 27.475038301019598 18.372602884462992 31.923239473823234 24-25 27.735221089333827 25.184901473928896 20.50029055945903 26.57958687727825 26-27 28.175022452321834 29.2421681018543 19.207300966770564 23.375508479053305 28-29 22.69401447514396 26.640340218712026 21.854033493581277 28.81161181256273 30-31 23.302868614295523 24.018701463363094 25.092450736964445 27.585979185376935 32-33 20.62179724232659 25.104337260288446 22.93702784087907 31.33683765650589 34-35 21.554228960853717 20.456706640604363 27.700882244175602 30.288182154366318 36-37 29.619895398594746 22.885519573141742 22.97532886047863 24.51925616778488 38-39 22.495905753077288 24.62623487770088 27.53711236726716 25.34074700195467 40-41 23.357018331660413 18.688256114955887 27.629563104231604 30.325162449152092 42-43 28.985947487981406 17.975064715515877 26.46732526810714 26.571662528395585 44-45 21.3468751650906 24.539067039991547 30.20629721591209 23.907760579005757 46-47 25.99186433514713 20.094828041629246 28.237096518569395 25.676211104654236 48-49 25.846584605631573 18.07411907654921 23.53399545670664 32.54530086111258 50-51 24.55623646257066 18.99862644619367 26.867504886681807 29.57763220455386 52-53 19.377674467747898 18.614295525384332 37.81499286808601 24.193037138781765 54-55 21.069522954197264 21.057636430873263 29.806117597337416 28.066723017592054 56-57 24.539067039991547 23.378149928680863 24.167943367319985 27.91483966400761 58-59 20.384066775846584 23.8575730360822 31.30514026097522 24.45321992709599 60-61 24.60510328068044 28.44973321358762 24.630197052142215 22.31496645358973 62-63 19.422579111416347 37.93517882613979 20.010301653547465 22.631940408896405 64-65 18.241851127898993 39.720798774367374 19.579745364255903 22.45760473347773 66-67 18.070156902107875 38.38950816207935 19.884832796238573 23.655502139574196 68-69 17.78488034233187 38.57176818638068 19.870304823287018 23.773046648000424 70-71 18.25505837603677 36.589360240900206 20.30746473664747 24.84811664641555 72-73 18.57335305615722 33.1567964498917 20.999524539067043 27.27032595488404 74-75 18.010724285487875 32.22040255692324 21.73913043478261 28.02974272280628 76 18.42146970257277 32.51624491520947 21.30064979660838 27.761635585609383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 2.0 17 3.0 18 4.0 19 3.5 20 5.0 21 8.0 22 11.5 23 20.0 24 29.5 25 34.0 26 47.0 27 70.0 28 119.0 29 158.0 30 159.0 31 203.0 32 304.5 33 363.0 34 412.5 35 517.0 36 602.0 37 632.0 38 752.0 39 1077.5 40 2008.5 41 3131.0 42 3528.0 43 3392.5 44 2978.5 45 2388.5 46 2077.0 47 1928.0 48 1569.5 49 1285.5 50 1211.0 51 1137.0 52 911.5 53 751.0 54 742.0 55 698.0 56 638.0 57 598.0 58 574.0 59 579.5 60 609.0 61 659.5 62 686.0 63 720.0 64 783.5 65 863.0 66 934.0 67 955.0 68 944.0 69 918.5 70 887.0 71 870.0 72 747.0 73 545.5 74 411.5 75 356.0 76 282.0 77 143.0 78 50.5 79 23.0 80 16.5 81 6.5 82 1.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37858.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.845528025780546 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2361176804465 41.45755190448518 2 15.954025163182292 17.819219187490095 3 5.193453788667108 8.700935073168155 4 2.0669756882035757 4.6172539489671935 5 0.9317945322107652 2.6018278831422688 6 0.6054299498628323 2.028633313962703 7 0.35947403273105666 1.4052512018595804 8 0.20811654526534862 0.9297902688995721 9 0.09932835114937091 0.4992339796080089 >10 0.27906536751489924 2.6018278831422688 >50 0.023649607416516883 0.9826192614506842 >100 0.028379528899820265 3.359923926250726 >500 0.004729921483303377 1.767129800834698 >1k 0.009459842966606754 11.228802366738865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 2648 6.994558613767235 No Hit CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG 1603 4.234243752971631 RNA PCR Primer, Index 32 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 669 1.767129800834698 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 420 1.1094088435733531 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 306 0.8082835860320143 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 175 0.4622536848222304 RNA PCR Primer, Index 42 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 132 0.3486713508373395 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 120 0.31697395530667233 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 119 0.3143325056791167 No Hit CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 96 0.2535791642453379 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 87 0.2298061175973374 No Hit CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 75 0.19810872206667018 No Hit CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 59 0.15584552802578056 No Hit TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT 55 0.14527972951555815 RNA PCR Primer, Index 42 (95% over 21bp) ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT 46 0.12150668286755771 RNA PCR Primer, Index 42 (95% over 24bp) CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 42 0.1109408843573353 No Hit CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 40 0.1056579851022241 RNA PCR Primer, Index 32 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT 38 0.1003750858471129 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0026414496275556027 0.0 2 0.0 0.0 0.0 0.007924348882666807 0.0 3 0.0 0.0 0.0 0.007924348882666807 0.0 4 0.0 0.0 0.0 0.01056579851022241 0.0 5 0.0 0.0026414496275556027 0.0 0.01056579851022241 0.0 6 0.0 0.0026414496275556027 0.0 0.01056579851022241 0.0 7 0.0 0.0026414496275556027 0.0 0.01056579851022241 0.0 8 0.0 0.0026414496275556027 0.0 0.01056579851022241 0.0 9 0.0 0.0026414496275556027 0.0 0.013207248137778013 0.0 10 0.0 0.0026414496275556027 0.0 0.018490147392889216 0.0 11 0.0 0.0026414496275556027 0.0 0.02113159702044482 0.0 12 0.0 0.0026414496275556027 0.0 0.02113159702044482 0.0 13 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 14 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 15 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 16 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 17 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 18 0.0 0.0026414496275556027 0.0 0.026414496275556025 0.0 19 0.0 0.0026414496275556027 0.0 0.04226319404088964 0.0 20 0.0 0.0026414496275556027 0.0 0.047546093296000846 0.0 21 0.0 0.0026414496275556027 0.0 0.05547044217866765 0.0 22 0.0 0.0026414496275556027 0.0 0.058111891806223254 0.0 23 0.0 0.0026414496275556027 0.0 0.060753341433778855 0.0 24 0.0 0.0026414496275556027 0.0 0.06603624068889007 0.0 25 0.0 0.0026414496275556027 0.0 0.06867769031644566 0.0 26 0.0 0.0026414496275556027 0.0 0.07924348882666808 0.0 27 0.0 0.0026414496275556027 0.0 0.11358233398489091 0.0 28 0.0 0.0026414496275556027 0.0 0.13999683026044693 0.0 29 0.0 0.0026414496275556027 0.0 0.17697712504622537 0.0 30 0.0 0.0026414496275556027 0.0 0.23773046648000423 0.0 31 0.0 0.0026414496275556027 0.0 0.2932009086586719 0.0 32 0.0 0.0026414496275556027 0.0 0.34602990120978394 0.0 33 0.0 0.0026414496275556027 0.0 0.4279148396640076 0.0 34 0.0 0.0026414496275556027 0.0 0.5230070262560093 0.0 35 0.0 0.0026414496275556027 0.0 0.771303291246236 0.0 36 0.0 0.0026414496275556027 0.0 1.0222410058640181 0.0 37 0.0 0.0026414496275556027 0.0 1.481853241058693 0.0 38 0.0 0.0026414496275556027 0.0 1.8463732896613663 0.0 39 0.0 0.0026414496275556027 0.0 2.195044640498706 0.0 40 0.0 0.0026414496275556027 0.0 2.60711078239738 0.0 41 0.0 0.0026414496275556027 0.0 3.082571715357388 0.0 42 0.0 0.0026414496275556027 0.0 3.642559036399176 0.0 43 0.0 0.0026414496275556027 0.0 4.236885202599186 0.0 44 0.0 0.0026414496275556027 0.0 4.717629034814306 0.0 45 0.0 0.0026414496275556027 0.0 5.203655766284537 0.0 46 0.0 0.0026414496275556027 0.0 5.668550900734323 0.0 47 0.0 0.0026414496275556027 0.0 6.220613872893444 0.0 48 0.0 0.0026414496275556027 0.0 6.690791906598341 0.0 49 0.0 0.0026414496275556027 0.0 7.155687041048127 0.0 50 0.0 0.0026414496275556027 0.0 7.591526229594802 0.0 51 0.0 0.0026414496275556027 0.0 8.127740503988589 0.0 52 0.0 0.0026414496275556027 0.0 8.603201436948597 0.0 53 0.0 0.0026414496275556027 0.0 9.20281050240372 0.0 54 0.0 0.0026414496275556027 0.0 9.802419567858841 0.0 55 0.0 0.0026414496275556027 0.0 10.335992392625073 0.0 56 0.0 0.0026414496275556027 0.0 10.87220666701886 0.0 57 0.0 0.0026414496275556027 0.0 11.411062391040202 0.0 58 0.0 0.0026414496275556027 0.0 11.836335781076654 0.0 59 0.0 0.0026414496275556027 0.0 12.272174969623329 0.0 60 0.0 0.0026414496275556027 0.0 12.763484600348672 0.0 61 0.0 0.0026414496275556027 0.0 13.24422843256379 0.0 62 0.0 0.0026414496275556027 0.0 13.814781552115802 0.0 63 0.0 0.0026414496275556027 0.0 14.33778857837181 0.0 64 0.0 0.0026414496275556027 0.0 14.802683712821597 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAATAC 15 0.0022082732 70.0 3 TCTTTAT 65 0.0 59.230766 1 CTTTATA 70 0.0 55.0 2 ATTTATG 20 0.006900469 52.5 18 AACTCGT 30 5.807244E-4 46.666664 37 TTTATAC 95 0.0 44.210526 3 ATGGCCT 35 0.0012404487 40.0 18 CGAACTC 45 0.0042565307 31.111113 35 GAACTCG 45 0.0042565307 31.111113 36 ACTCGTA 50 0.0071239877 27.999998 38 TGCTTGA 730 0.0 27.328766 55 TTGAAAA 730 0.0 27.328766 58 TGAAAAA 735 0.0 27.142855 59 CGTCTTC 725 0.0 27.034483 48 TTCTGCT 725 0.0 27.034483 52 GAAAAAA 740 0.0 26.95946 60 GTCTTCT 730 0.0 26.849314 49 CTTGAAA 745 0.0 26.778526 57 CTGCTTG 720 0.0 26.736113 54 TCTTCTG 720 0.0 26.736113 50 >>END_MODULE