FastQCFastQC Report
Wed 25 May 2016
SRR1780201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267368
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATC250.002338949435.23237240
GGTCCAA300.00575861429.3108921
TGAGTGC450.001393984424.44860528
ATATCGG450.001398553124.43488142
GCCTAGA500.00258670321.9831682
GACGAAA654.9313455E-420.2997523
ATCACTT550.00447022620.00340526
TATTAGA550.004494503619.9846972
GTCAAGG550.004494503619.9846972
GAGACAG1051.7951625E-618.8427167
ATAAGAG708.1425346E-418.8427143
CGACGAA1509.544237E-917.59311522
GTATTAG750.001293928617.5865351
TCTAGAG750.001293928617.5865353
AGAGACA750.001293928617.5865356
CGCTGCC3950.017.27522916
TGACGCT3550.016.7258513
GACGAGA1854.1836756E-1016.64213623
CATCTGA4250.016.5520329
TATAAGA800.001993069716.4873752
Produced by FastQC (version 0.11.5)