Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780201_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 267368 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 61 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT | 13797 | 5.160303402052602 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG | 3749 | 1.4021872475389725 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC | 3426 | 1.2813799706771192 | No Hit |
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG | 2967 | 1.109706471978696 | Illumina PCR Primer Index 4 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC | 1025 | 0.38336674545944166 | No Hit |
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT | 456 | 0.17055144968732233 | RNA PCR Primer, Index 27 (95% over 21bp) |
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG | 273 | 0.10210646001017326 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCTTATA | 25 | 4.4240867E-5 | 44.01609 | 1 |
TCGTTTA | 20 | 7.845962E-4 | 43.999622 | 32 |
TTGCTAA | 20 | 7.845962E-4 | 43.999622 | 39 |
TCTTCTG | 330 | 0.0 | 39.333 | 44 |
GTCTTCT | 350 | 0.0 | 37.0854 | 43 |
CCTCAAA | 25 | 0.0023496249 | 35.1997 | 14 |
GTATAGC | 25 | 0.0023496249 | 35.1997 | 13 |
CGTTTAT | 25 | 0.0023496249 | 35.1997 | 33 |
TCTTTAT | 145 | 0.0 | 34.90931 | 1 |
CTTTATA | 155 | 0.0 | 32.644882 | 2 |
ATGCCGT | 2035 | 0.0 | 32.540264 | 44 |
CGTCTTC | 405 | 0.0 | 32.049107 | 42 |
TTTATAC | 175 | 0.0 | 31.428305 | 3 |
CCGTCTT | 445 | 0.0 | 29.66267 | 41 |
TTTCGTT | 30 | 0.0057373554 | 29.333082 | 30 |
GCTTAGT | 30 | 0.0057373554 | 29.333082 | 18 |
TTCGGTC | 30 | 0.0057373554 | 29.333082 | 28 |
TTCTGAT | 30 | 0.0057373554 | 29.333082 | 19 |
ATCCCTA | 40 | 7.018068E-4 | 27.499765 | 39 |
GCCGTCT | 490 | 0.0 | 26.489573 | 40 |