FastQCFastQC Report
Wed 25 May 2016
SRR1780201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267368
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT137975.160303402052602No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG37491.4021872475389725No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC34261.2813799706771192No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG29671.109706471978696Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC10250.38336674545944166No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT4560.17055144968732233RNA PCR Primer, Index 27 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG2730.10210646001017326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA254.4240867E-544.016091
TCGTTTA207.845962E-443.99962232
TTGCTAA207.845962E-443.99962239
TCTTCTG3300.039.33344
GTCTTCT3500.037.085443
CCTCAAA250.002349624935.199714
GTATAGC250.002349624935.199713
CGTTTAT250.002349624935.199733
TCTTTAT1450.034.909311
CTTTATA1550.032.6448822
ATGCCGT20350.032.54026444
CGTCTTC4050.032.04910742
TTTATAC1750.031.4283053
CCGTCTT4450.029.6626741
TTTCGTT300.005737355429.33308230
GCTTAGT300.005737355429.33308218
TTCGGTC300.005737355429.33308228
TTCTGAT300.005737355429.33308219
ATCCCTA407.018068E-427.49976539
GCCGTCT4900.026.48957340