FastQCFastQC Report
Wed 25 May 2016
SRR1780200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71374
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATA200.006925476252.520
TACGACA200.006925476252.549
GGCATAA200.006925476252.521
GATCTCG200.006925476252.541
AGATCTC305.8422016E-446.66666840
GTGTAGA556.6113553E-638.1818236
TCTCGGT501.6944873E-435.00000443
TGTAGAT501.6944873E-435.00000437
TAGATCT400.002404308235.039
TATACAA400.002404308235.010
TATGAGG450.00428169131.11111316
GCATAAT500.007165839428.00000222
CGGTGGT500.007165839428.00000246
AGGCAAC1750.001001608614.00000170
GGCAACC1500.005863521714.070
GAGGCAA2052.1180467E-413.65853770
GACGAGG3658.312782E-913.42465770
CGACGAG3902.102206E-812.56410269
CGAGGCA2305.598995E-412.17391369
CCGACGA5057.4859236E-79.702970568
Produced by FastQC (version 0.11.5)