##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71374 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.917967327037857 34.0 33.0 34.0 21.0 34.0 2 31.134194524616806 34.0 34.0 34.0 21.0 34.0 3 30.991215288480397 34.0 34.0 34.0 21.0 34.0 4 30.86583349679155 34.0 33.0 34.0 21.0 34.0 5 30.917126684787178 34.0 33.0 34.0 21.0 34.0 6 33.852103006697114 38.0 34.0 38.0 21.0 38.0 7 34.236752879199706 38.0 36.0 38.0 21.0 38.0 8 34.11874071790848 38.0 36.0 38.0 21.0 38.0 9 34.382772438142744 38.0 36.0 38.0 21.0 38.0 10-11 34.20510970381372 38.0 36.0 38.0 21.0 38.0 12-13 34.26211225376187 38.0 36.0 38.0 21.0 38.0 14-15 34.22233586460055 38.0 36.0 38.0 21.0 38.0 16-17 34.24591587973212 38.0 36.0 38.0 21.0 38.0 18-19 34.28913890212122 38.0 36.0 38.0 21.0 38.0 20-21 34.28310729397259 38.0 36.0 38.0 21.5 38.0 22-23 34.278560820466836 38.0 36.0 38.0 21.0 38.0 24-25 34.33627791632807 38.0 36.0 38.0 21.0 38.0 26-27 34.321076302294955 38.0 36.0 38.0 21.0 38.0 28-29 34.40171491019139 38.0 36.0 38.0 21.5 38.0 30-31 34.40481828116681 38.0 36.0 38.0 21.5 38.0 32-33 34.441070978227366 38.0 37.0 38.0 21.5 38.0 34-35 34.41980973463727 38.0 36.0 38.0 22.0 38.0 36-37 34.35781236864965 38.0 36.0 38.0 21.5 38.0 38-39 34.37722419928825 38.0 36.0 38.0 21.5 38.0 40-41 34.4337924174069 38.0 36.5 38.0 22.0 38.0 42-43 34.48518368033177 38.0 37.0 38.0 22.0 38.0 44-45 34.53043825482669 38.0 37.0 38.0 23.0 38.0 46-47 34.47592961022221 38.0 37.0 38.0 22.0 38.0 48-49 34.45549499817861 38.0 37.0 38.0 21.5 38.0 50-51 34.4245663687057 38.0 37.0 38.0 21.5 38.0 52-53 34.47464062543783 38.0 37.0 38.0 22.0 38.0 54-55 34.42577829461709 38.0 37.0 38.0 22.0 38.0 56-57 34.416068876621736 38.0 36.5 38.0 22.0 38.0 58-59 34.46801356236165 38.0 37.0 38.0 22.5 38.0 60-61 34.48485442878359 38.0 37.0 38.0 23.0 38.0 62-63 34.36829237537479 38.0 36.0 38.0 22.0 38.0 64-65 34.34890856614453 38.0 36.0 38.0 22.0 38.0 66-67 34.29973099447979 38.0 36.0 38.0 22.0 38.0 68-69 34.258770700815425 38.0 36.0 38.0 22.0 38.0 70-71 34.188808249502614 38.0 36.0 38.0 21.0 38.0 72-73 34.246196093815676 38.0 36.0 38.0 21.5 38.0 74-75 34.28852943648948 38.0 36.0 38.0 22.0 38.0 76 33.04609521674559 37.0 33.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0011929171654010418 1101 2 -0.12637644173481632 1101 3 0.007252884158802431 1101 4 -0.06417550121442872 1101 5 -0.7061794745892698 1101 6 0.07179399407748122 1101 7 -0.800482184573788 1101 8 0.04326449876447924 1101 9 -0.5777776208735972 1101 10-11 -0.5523308546255663 1101 12-13 -0.590919159939169 1101 14-15 -0.37047875282850384 1101 16-17 -0.5175512434639415 1101 18-19 -0.6010233613778482 1101 20-21 -0.6867038086038662 1101 22-23 -0.6646377338552512 1101 24-25 -0.42991968984007656 1101 26-27 -0.7137754504172307 1101 28-29 -0.549658437984121 1101 30-31 -0.6007474128557249 1101 32-33 -0.4193248164566228 1101 34-35 -0.30964950994734153 1101 36-37 -0.5600170584902742 1101 38-39 -0.5807685173368355 1101 40-41 -0.691250080264453 1101 42-43 -0.7355393094955005 1101 44-45 -0.7371314950983674 1101 46-47 -0.6525946717489433 1101 48-49 -0.7534550468082202 1101 50-51 -1.1366298397040495 1101 52-53 -0.7207167833447272 1101 54-55 -0.7862828227739556 1101 56-57 -0.494641881391793 1101 58-59 -0.5299446969749155 1101 60-61 -0.5650394659111484 1101 62-63 -0.5823529884641232 1101 64-65 -0.6022553291309052 1101 66-67 -0.5341009428216594 1101 68-69 -0.7546800464203969 1101 70-71 -0.6864553839413503 1101 72-73 -0.7416706351478197 1101 74-75 -0.7044268410686882 1101 76 -1.7171469479450643 1102 1 0.38085476866910284 1102 2 -0.20446199573201085 1102 3 0.5009415237099972 1102 4 0.4389100527827665 1102 5 -0.49604623475757137 1102 6 0.343252619603426 1102 7 -0.36426198822034905 1102 8 0.02576800507583954 1102 9 0.1449432767421115 1102 10-11 -0.30203281815992966 1102 12-13 0.20068322435255936 1102 14-15 0.18970708167359618 1102 16-17 -0.09598742859718357 1102 18-19 0.18418000468105333 1102 20-21 -0.15798711856179182 1102 22-23 -0.15957111393939982 1102 24-25 -0.0037976702047330946 1102 26-27 -0.03034277159535037 1102 28-29 -0.2545848054455462 1102 30-31 -0.06848934833959675 1102 32-33 0.13779299560508917 1102 34-35 -0.4846495099473387 1102 36-37 -0.03140555778901444 1102 38-39 -0.4116941835359995 1102 40-41 0.03289087345223152 1102 42-43 -0.09355123095412665 1102 44-45 -0.12740148107311455 1102 46-47 -0.27999649503084356 1102 48-49 -0.07415981539166694 1102 50-51 -0.18312352834360013 1102 52-53 0.17391856027378338 1102 54-55 -0.01231718462528164 1102 56-57 -0.17307806652502933 1102 58-59 0.07894380232382048 1102 60-61 0.34235885105940156 1102 62-63 0.38076061602395583 1102 64-65 0.15137370312715603 1102 66-67 -0.047635304673001144 1102 68-69 -0.3385334826055342 1102 70-71 -0.2447302787520087 1102 72-73 0.2692339931831711 1102 74-75 -0.21699696729589846 1102 76 -0.41234330138124875 1103 1 -0.37035327625710934 1103 2 -0.09066089869661909 1103 3 -0.0958979695641311 1103 4 -0.522563109029953 1103 5 -0.5381808827920516 1103 6 -0.9787076781633246 1103 7 -0.7727933973884333 1103 8 -0.5194357300684729 1103 9 -0.20441025597305185 1103 10-11 -0.4937936252645372 1103 12-13 0.14454994761232598 1103 14-15 -0.24278116437576358 1103 16-17 -0.2948087685475542 1103 18-19 -0.11678643125991073 1103 20-21 -0.21514070351673098 1103 22-23 -0.0612052395760756 1103 24-25 -0.18204959654664066 1103 26-27 -0.3908217450836631 1103 28-29 -0.15401506533845577 1103 30-31 -0.3355652266209077 1103 32-33 -0.18702416516283904 1103 34-35 -0.7756414127813471 1103 36-37 -0.44449162282754173 1103 38-39 -0.5305396843848911 1103 40-41 -0.44406648582685904 1103 42-43 -0.6904152116254139 1103 44-45 -0.4025337130177391 1103 46-47 -0.11832253656147884 1103 48-49 -0.04564832263994134 1103 50-51 -0.7421218305507367 1103 52-53 -0.38147193208614993 1103 54-55 -0.7144116733284349 1103 56-57 -0.7706848315766166 1103 58-59 -0.6974944241356269 1103 60-61 -0.16353444918873805 1103 62-63 -0.5162290666193741 1103 64-65 -0.1008383250823286 1103 66-67 -0.38409214155428373 1103 68-69 -0.26919333633414766 1103 70-71 -0.2440772814945973 1103 72-73 0.0818727551003704 1103 74-75 -0.2949566773651142 1103 76 0.08111040111320023 1104 1 0.3356163674353283 1104 2 0.2284997058487619 1104 3 -0.2669546335962636 1104 4 0.1538567177358594 1104 5 -0.35572044115777857 1104 6 0.06664935524151616 1104 7 0.28004786912446633 1104 8 0.2050348088285716 1104 9 0.7420387657538043 1104 10-11 0.11268733349152882 1104 12-13 0.30663843576497385 1104 14-15 0.3947819435165556 1104 16-17 -0.3416981341620158 1104 18-19 0.6284673713434401 1104 20-21 -0.03905960281831966 1104 22-23 0.5759304282788307 1104 24-25 0.4547070207090371 1104 26-27 0.563740572447827 1104 28-29 0.5520997466685742 1104 30-31 0.3494517903315284 1104 32-33 0.48117752201557096 1104 34-35 0.34254172511241876 1104 36-37 0.27575290513348705 1104 38-39 0.5108736098067652 1104 40-41 0.22382873490963107 1104 42-43 0.2262743056092944 1104 44-45 0.5912159845482634 1104 46-47 0.7247827749858544 1104 48-49 0.32971661437314026 1104 50-51 0.5986204634312386 1104 52-53 0.4868442767626675 1104 54-55 0.3014358676816187 1104 56-57 0.16288453468303743 1104 58-59 0.28548055852482435 1104 60-61 0.33983668167243053 1104 62-63 0.22605685044112533 1104 64-65 0.16686817684519184 1104 66-67 0.4834982257215543 1104 68-69 0.2276774799956769 1104 70-71 0.17764473731276098 1104 72-73 0.3283362936586869 1104 74-75 0.11275659908374536 1104 76 0.29423361675267046 1105 1 -0.10381598579637341 1105 2 0.20732819355084686 1105 3 -0.2573895879935506 1105 4 -0.2796983183182711 1105 5 0.21699695372549854 1105 6 -0.004454658455756544 1105 7 -0.08923532866290174 1105 8 0.1208426518367034 1105 9 0.1253485020153704 1105 10-11 -0.10860106662700986 1105 12-13 -0.9116701480814555 1105 14-15 -0.8286713507444219 1105 16-17 -0.6999263782106198 1105 18-19 -0.48950701211445846 1105 20-21 -0.5100598991536316 1105 22-23 -0.16461976763174846 1105 24-25 0.17318846682539402 1105 26-27 -0.7255073943626087 1105 28-29 -0.42795523999633645 1105 30-31 -0.1668631153350617 1105 32-33 -0.11377325339518052 1105 34-35 0.15894553137497525 1105 36-37 -0.4330317907935495 1105 38-39 -0.2407397713792392 1105 40-41 -0.42310419452963544 1105 42-43 -0.017321558866690623 1105 44-45 -0.018913744469543303 1105 46-47 -0.28784440225558683 1105 48-49 -0.622696872176526 1105 50-51 -0.05888999062032951 1105 52-53 -0.05578685161637509 1105 54-55 -0.140433020402412 1105 56-57 -0.09933106572524508 1105 58-59 -0.24412004731627235 1105 60-61 -0.38381416946846514 1105 62-63 -0.3251844652876983 1105 64-65 -0.3663049159077687 1105 66-67 -0.31056519003689687 1105 68-69 -0.3953016777534941 1105 70-71 -0.20147514678720313 1105 72-73 -0.40295342350570706 1105 74-75 -0.10144444796771523 1105 76 0.30789796761368393 1106 1 0.011546392192265387 1106 2 -0.029382346189997577 1106 3 -0.2895801807902032 1106 4 0.16969460077161713 1106 5 -0.46738899328671124 1106 6 -0.4494262599930181 1106 7 -0.7615306726754127 1106 8 -1.0126131179827027 1106 9 -0.9351045129831022 1106 10-11 -0.6531628460372687 1106 12-13 -0.38707231792806596 1106 14-15 -1.0494821523633036 1106 16-17 -0.33073786003220107 1106 18-19 -0.5861638158419709 1106 20-21 -0.21183352769494235 1106 22-23 -1.001209571393268 1106 24-25 -0.3438846209547606 1106 26-27 -0.35417623767791895 1106 28-29 -0.20024888350209835 1106 30-31 -0.5513646968063455 1106 32-33 -0.5480061547998005 1106 34-35 -0.28248901612017363 1106 36-37 -0.17404461261445192 1106 38-39 -0.281135309249521 1106 40-41 -0.46316813358347275 1106 42-43 -0.5014813384810068 1106 44-45 -0.2479294911620613 1106 46-47 -0.2669621793531576 1106 48-49 -0.12061375140653752 1106 50-51 -0.6702136650753161 1106 52-53 -0.48413599966249876 1106 54-55 -0.9674064584869626 1106 56-57 -0.3358120370547013 1106 58-59 -0.4021311346202907 1106 60-61 -0.022983641971265456 1106 62-63 -0.34879600152549983 1106 64-65 -0.18805779826670488 1106 66-67 -0.02304350857582449 1106 68-69 -0.35032327418922904 1106 70-71 -0.11589714454252942 1106 72-73 -0.28587353369854895 1106 74-75 -0.10703911517854436 1106 76 -0.8139263360287998 1107 1 -0.6452262196630869 1107 2 -0.5856733890058621 1107 3 -0.45842704183564464 1107 4 -0.35381472415614823 1107 5 -0.552617957475487 1107 6 -0.03893681073288491 1107 7 0.07065565076386093 1107 8 -0.07486686108751428 1107 9 -0.4328192489957061 1107 10-11 -0.6498794207680234 1107 12-13 -0.2301421016597871 1107 14-15 -0.46867486753711063 1107 16-17 -0.1424356097914199 1107 18-19 -0.28136563704852335 1107 20-21 -0.0453069538397628 1107 22-23 0.08235579065908638 1107 24-25 -0.03089101268928829 1107 26-27 -0.0728873838122297 1107 28-29 0.12383832427305208 1107 30-31 -0.20287507243019576 1107 32-33 -0.20683637982387637 1107 34-35 -0.3181601482452052 1107 36-37 -0.297019833698414 1107 38-39 -0.32281292066709 1107 40-41 0.09455010474941616 1107 42-43 0.02334135655444669 1107 44-45 -0.49739976521862417 1107 46-47 -0.119847215689731 1107 48-49 0.2047780706755873 1107 50-51 -0.18075564913976194 1107 52-53 -0.12122557150384239 1107 54-55 -0.3566806026074403 1107 56-57 -0.0025049711235212158 1107 58-59 0.0847176527013076 1107 60-61 -0.04242151956517404 1107 62-63 0.36699946296972996 1107 64-65 0.23710637299675597 1107 66-67 -0.06620085031472911 1107 68-69 0.23232272878774296 1107 70-71 0.09185173909287414 1107 72-73 -0.29296573228010914 1107 74-75 0.36883865384343295 1107 76 0.1982092037663179 1108 1 0.6665690543833875 1108 2 0.7373582807656831 1108 3 0.5147664085026236 1108 4 0.7660989928749338 1108 5 0.2890920140903539 1108 6 0.876662757852273 1108 7 1.0038255693833378 1108 8 0.3777265304214623 1108 9 0.449090742179898 1108 10-11 0.8923796242363906 1108 12-13 0.6255104132926519 1108 14-15 0.6272070816735962 1108 16-17 0.690302893983457 1108 18-19 0.4652859954644697 1108 20-21 0.5971972132354395 1108 22-23 0.6638735865214258 1108 24-25 0.5774235279519502 1108 26-27 0.9250143712617955 1108 28-29 0.2664405401765748 1108 30-31 0.40155673460971286 1108 32-33 0.5358344702594593 1108 34-35 0.5147744531862983 1108 36-37 0.5768893730865585 1108 38-39 0.3855408395054809 1108 40-41 0.449653546263292 1108 42-43 0.746598538631126 1108 44-45 0.2749602700789566 1108 46-47 0.5929873759368931 1108 48-49 0.6000913366820626 1108 50-51 0.7258626467716027 1108 52-53 0.1226512791677905 1108 54-55 0.5847450273562842 1108 56-57 0.5671293067468568 1108 58-59 0.8776189175311941 1108 60-61 0.5606768234096293 1108 62-63 0.5639403395262548 1108 64-65 0.6251640257078037 1108 66-67 0.626327828967554 1108 68-69 0.5321462408967719 1108 70-71 0.649278937837849 1108 72-73 0.7202708595426159 1108 74-75 0.5160099451465001 1108 76 0.5294769751164452 1109 1 -0.8638615828325662 1109 2 -0.6729388748039966 1109 3 -0.5673883666443658 1109 4 -0.4417509991797317 1109 5 -0.1971625544778952 1109 6 -0.8862484132902679 1109 7 -0.3486295394677654 1109 8 -0.6438847837371284 1109 9 -0.8258178864550914 1109 10-11 -0.6711036111044635 1109 12-13 -1.2348900316461666 1109 14-15 -0.5940705283693077 1109 16-17 -0.06593419484539709 1109 18-19 -0.23573100755476162 1109 20-21 -0.6444999105528879 1109 22-23 -0.7022475004005528 1109 24-25 -0.4977393514378434 1109 26-27 -0.4624796697361404 1109 28-29 -0.7258524838183433 1109 30-31 -0.3076439645798601 1109 32-33 -0.5042894769835016 1109 34-35 -0.42738350009512516 1109 36-37 -0.6592460154403739 1109 38-39 -0.9238505477502059 1109 40-41 -0.7694788230359251 1109 42-43 -0.8454879066255145 1109 44-45 -0.7510209789278761 1109 46-47 -0.7011489660303738 1109 48-49 -0.254429559551852 1109 50-51 -0.6056167748079275 1109 52-53 -0.38600913716781804 1109 54-55 -0.4299538444059934 1109 56-57 -0.7711036505072357 1109 58-59 -0.7245581045568414 1109 60-61 -0.5418974654827906 1109 62-63 -1.1859404804324214 1109 64-65 -0.761614935042239 1109 66-67 -0.7851611270152787 1109 68-69 -0.46062350326340606 1109 70-71 -0.3813900594607347 1109 72-73 -0.9722446338627506 1109 74-75 -0.9929682846326173 1109 76 -0.8717624973132345 1110 1 -0.306052252203763 1110 2 -0.05843100268963397 1110 3 0.24872773496869272 1110 4 0.006114353858034605 1110 5 0.35238830034679225 1110 6 0.44097557975522506 1110 7 0.4498588213938177 1110 8 0.3381494092504127 1110 9 0.06761879856374264 1110 10-11 -0.01554483587023725 1110 12-13 0.5288378733089161 1110 14-15 0.17361758135734107 1110 16-17 -0.01652822556113165 1110 18-19 0.2410269370023741 1110 20-21 0.4001248358974081 1110 22-23 -0.0907460032499614 1110 24-25 -0.06870911865507878 1110 26-27 -0.04105592782558176 1110 28-29 0.09096389790676085 1110 30-31 0.30366435185015916 1110 32-33 -0.046583980992004115 1110 34-35 0.18446813711148735 1110 36-37 0.2385976047663263 1110 38-39 0.26912491829828866 1110 40-41 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12-13 0.7336353660765198 2116 14-15 0.8913530361950208 2116 16-17 0.7218999325873767 2116 18-19 0.8835454198053299 2116 20-21 0.37698115219442485 2116 22-23 0.6342218527448935 2116 24-25 0.4916412510009849 2116 26-27 0.4874718767388657 2116 28-29 0.35550906023398454 2116 30-31 -0.08025560957703703 2116 32-33 -0.24302403242241155 2116 34-35 0.10756360480676364 2116 36-37 0.3820000477107186 2116 38-39 0.6152756736824045 2116 40-41 0.5462766481746044 2116 42-43 0.7990651096064241 2116 44-45 0.9327188256429011 2116 46-47 0.6307980581156301 2116 48-49 -0.038629672610071 2116 50-51 0.6927358053422736 2116 52-53 0.5889767305540516 2116 54-55 0.4214149676750836 2116 56-57 0.030055196298874876 2116 58-59 0.08049721321752656 2116 60-61 -0.06265172535519525 2116 62-63 0.43311386827144105 2116 64-65 0.1075807364428627 2116 66-67 0.4342525853322954 2116 68-69 0.34592579819827307 2116 70-71 0.33168431670013376 2116 72-73 0.05565652094626472 2116 74-75 0.9120022394730043 2116 76 0.5406577562602095 2117 1 0.36725839436891405 2117 2 0.5709645571872102 2117 3 0.5153376847337228 2117 4 0.49302895440899874 2117 5 0.31617050744450736 2117 6 0.4707519531144868 2117 7 -0.027251857588517225 2117 8 0.15390050307637182 2117 9 0.5261749482963651 2117 10-11 0.6785890160176251 2117 12-13 0.5490736535714333 2117 14-15 0.5390972442969044 2117 16-17 0.47982568790508395 2117 18-19 0.4691706738359471 2117 20-21 0.20275001820173344 2117 22-23 0.6680248604674262 2117 24-25 0.020295904841923118 2117 26-27 -0.08294541089153995 2117 28-29 -0.029194909417824988 2117 30-31 0.21743440532608815 2117 32-33 0.32630939123291824 2117 34-35 0.17960668839976535 2117 36-37 0.9016789530081013 2117 38-39 0.4555412203562952 2117 40-41 0.7421850616687138 2117 42-43 0.5301991022903394 2117 44-45 0.24348294974533502 2117 46-47 0.44356055642209924 2117 48-49 0.416559326170578 2117 50-51 0.8853248854127287 2117 52-53 0.4462792640861011 2117 54-55 0.38436036802734463 2117 56-57 0.4564540582416967 2117 58-59 0.4335659030969481 2117 60-61 0.1409792189612915 2117 62-63 0.39588991487759273 2117 64-65 -0.04399086632098914 2117 66-67 0.13984803310359695 2117 68-69 0.50965699993246 2117 70-71 0.37083890279957643 2117 72-73 -0.04758152267925908 2117 74-75 0.46053902310666217 2117 76 -0.22102765222102505 2118 1 0.5311836130161112 2118 2 0.24977383264255693 2118 3 1.0173837410604527 2118 4 1.0587033506217907 2118 5 0.8820856161663251 2118 6 0.5373490806118824 2118 7 0.9410543287229629 2118 8 0.9241002944588672 2118 9 0.49351828457173497 2118 10-11 0.48036226483412037 2118 12-13 0.36657345297768273 2118 14-15 0.7958572539253979 2118 16-17 0.9643398498243698 2118 18-19 0.2558993908382803 2118 20-21 0.2599192390955878 2118 22-23 0.3256113163769143 2118 24-25 0.4758844469264929 2118 26-27 0.8671869809884214 2118 28-29 0.9338156016950734 2118 30-31 0.6419710337462163 2118 32-33 0.1734569480009469 2118 34-35 0.5845252473342128 2118 36-37 0.8018233805135253 2118 38-39 0.729552293131718 2118 40-41 0.9141741493683 2118 42-43 0.7638908298037776 2118 44-45 0.9516190325504326 2118 46-47 1.0146911028394925 2118 48-49 0.5582587564843067 2118 50-51 0.7001367284681734 2118 52-53 0.7825546391964195 2118 54-55 1.3030797774793186 2118 56-57 0.9552103769450611 2118 58-59 1.0727779708173983 2118 60-61 0.7446059540972954 2118 62-63 1.1799306755291212 2118 64-65 0.5448320495098713 2118 66-67 0.3484655409267532 2118 68-69 0.9325086407893934 2118 70-71 1.0745619229391892 2118 72-73 1.21736271225614 2118 74-75 1.2186630702863184 2118 76 1.8113882989945722 2119 1 0.7952212468865945 2119 2 0.8825433251858499 2119 3 0.621327290254726 2119 4 1.0980387524834683 2119 5 1.1792530668719579 2119 6 0.8158997762379414 2119 7 1.4295940144401058 2119 8 1.3096472867519253 2119 9 1.373410187284179 2119 10-11 1.083505093379813 2119 12-13 1.209932476098487 2119 14-15 1.3648193197846723 2119 16-17 1.2396876628560918 2119 18-19 1.270312362512783 2119 20-21 1.2321442415978296 2119 22-23 1.4100372997619601 2119 24-25 1.5708198888242038 2119 26-27 1.0388915150690963 2119 28-29 1.559328563360566 2119 30-31 1.4250257830205655 2119 32-33 1.017116241364981 2119 34-35 1.3955051292279208 2119 36-37 1.2121927129126817 2119 38-39 0.9843816395434857 2119 40-41 1.7228019143567934 2119 42-43 1.1978313762956176 2119 44-45 1.6807752731669936 2119 46-47 1.5700409176728414 2119 48-49 1.3474190007041074 2119 50-51 1.6007973230032562 2119 52-53 1.1131021319398968 2119 54-55 1.30708338072224 2119 56-57 1.2553805300828813 2119 58-59 1.2921754285293474 2119 60-61 1.432024630906426 2119 62-63 1.648552435112748 2119 64-65 1.5405281760237344 2119 66-67 1.0365848483033915 2119 68-69 0.5292957491869501 2119 70-71 1.0888247594920841 2119 72-73 1.2020989748925146 2119 74-75 1.4875050280482967 2119 76 2.158200429912398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 10.0 13 152.0 14 603.0 15 976.0 16 1290.0 17 1382.0 18 1349.0 19 1098.0 20 868.0 21 598.0 22 433.0 23 298.0 24 279.0 25 302.0 26 319.0 27 379.0 28 406.0 29 539.0 30 683.0 31 886.0 32 1130.0 33 1715.0 34 2495.0 35 4185.0 36 9079.0 37 39920.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.94306049822064 21.71098719421638 14.543110936755681 27.802841370807297 2 18.691680443859106 21.77823857427074 33.32726202818954 26.202818953680612 3 18.033177347493485 24.639224367416706 27.65572897693838 29.67186930815143 4 15.081121977190573 18.871017457337405 31.51848011881077 34.52938044666125 5 15.172191554347522 33.126908958444254 32.82427774819963 18.876621739008602 6 28.609857931459636 32.626726819289935 19.502900215764846 19.260515033485586 7 27.355899907529352 28.52999691764508 21.076302294953344 23.037800879872222 8 23.91487096141452 31.281699218202707 21.56107265951187 23.242357160870906 9 23.347437442205845 16.993583097486482 21.879115644352286 37.77986381595539 10-11 25.7019362793174 23.93588701768151 25.10017653487264 25.262000168128452 12-13 24.68896236724858 21.47210468798162 24.85919242301118 28.97974052175862 14-15 23.841314764480064 22.450051839605457 22.78070445820607 30.92792893770841 16-17 24.6518340011769 24.377924734497157 23.69280130019335 27.2774399641326 18-19 23.74323983523412 23.662678286210664 24.81155602880601 27.782525849749206 20-21 23.61714349763219 24.00664107378037 24.682657550368482 27.69355787821896 22-23 24.91103202846975 23.67528791997086 24.26653963628212 27.14714041527727 24-25 24.166363101409477 24.86129402863788 23.502255723372656 27.470087146579985 26-27 23.343234230952447 24.94325664807913 24.6462297195057 27.06727940146272 28-29 23.839213158853365 24.19228290413876 25.646593997814332 26.321909939193546 30-31 24.122929918457704 23.555496399249026 25.63608596968084 26.68548771261244 32-33 23.16810042872755 24.698769860173172 25.098074929245946 27.035054781853336 34-35 23.315212822596465 24.24552358001513 25.82312887045703 26.616134726931374 36-37 24.1292347353378 23.539384089444333 25.145010788242217 27.18637038697565 38-39 22.79891837363746 23.488245019194665 27.159750049037463 26.553086558130413 40-41 23.087538879704095 23.194720766665732 26.862022585255136 26.855717768375037 42-43 22.04584302407039 23.629753131392384 26.623840614229273 27.700563230307957 44-45 21.57228122285426 23.396474906828818 27.4455684142685 27.58567545604842 46-47 21.78314232073304 23.941491299352705 27.812648863731894 26.462717516182366 48-49 21.699778630873986 23.786673018185894 27.561156723736936 26.95239162720318 50-51 21.554067307422873 24.62941687449211 26.625241684647072 27.191274133437947 52-53 20.64827528231569 24.508924818561383 27.128225964636982 27.714573934485948 54-55 20.87875136604366 24.306470143189397 27.04696388040463 27.76781461036232 56-57 20.901168492728445 24.221705382912546 26.82839689522795 28.04872922913106 58-59 21.142853139798806 24.244823044806232 26.499845882254043 28.112477933140916 60-61 21.005548238854484 23.505758399417154 26.943985204696386 28.544708157031973 62-63 20.89136099980385 24.03396194692745 26.58320957211309 28.491467481155603 64-65 21.668254546473506 24.478801804578698 25.224871802056768 28.628071846891025 66-67 21.38033457561577 24.86129402863788 24.50472160730798 29.25364978843837 68-69 21.25774091405834 25.26690391459075 24.697368789755373 28.77798638159554 70-71 22.44865076918766 24.854989211757783 24.623112057612015 28.073247961442544 72-73 22.354779051195113 24.520833917112675 24.438170762462523 28.686216269229693 74-75 21.458794519012525 25.537310505225992 24.453582537058313 28.55031243870317 76 21.498024490710904 24.782133550032228 23.92047524308572 29.799366716171154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.5 15 3.0 16 4.0 17 3.0 18 3.5 19 9.5 20 17.0 21 19.0 22 30.0 23 47.5 24 59.0 25 64.0 26 115.5 27 196.5 28 314.0 29 402.0 30 448.0 31 566.0 32 825.5 33 1013.0 34 1112.5 35 1387.0 36 1686.0 37 1810.0 38 2040.5 39 2536.5 40 3055.5 41 3496.0 42 3683.0 43 3661.0 44 3924.0 45 4051.0 46 3893.0 47 3870.5 48 3663.0 49 3275.5 50 3073.0 51 2931.0 52 2504.5 53 2037.5 54 1855.0 55 1842.0 56 1618.5 57 1373.0 58 1338.0 59 1326.5 60 1374.5 61 1480.5 62 1527.0 63 1648.0 64 1743.5 65 1776.0 66 1786.0 67 1738.0 68 1719.0 69 1646.5 70 1414.5 71 1236.0 72 1095.0 73 751.0 74 452.0 75 356.0 76 243.0 77 94.0 78 40.0 79 22.0 80 16.0 81 8.0 82 6.0 83 6.0 84 4.5 85 4.0 86 4.0 87 3.0 88 3.0 89 2.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.5 99 11.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 71374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.79390254154174 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11585866885785 49.96637430997282 2 12.158889417463133 15.756437918569787 3 4.367945335812827 8.490486731863143 4 2.158024477792674 5.5930731078544005 5 1.431475154607966 4.6375430829153474 6 0.8260173852873762 3.211253397595763 7 0.5146391039225015 2.3341833160534646 8 0.4108463434675432 2.129627035054782 9 0.27678069454655535 1.6140331213046768 >10 0.7395234182415776 6.2669879788158145 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002802140835598397 0.0 2 0.0 0.0 0.0 0.0042032112533975955 0.0 3 0.0 0.0 0.0 0.0042032112533975955 0.0 4 0.0 0.0 0.0 0.01821391543138958 0.0 5 0.0 0.0 0.0 0.022417126684787177 0.0 6 0.0 0.0 0.0 0.022417126684787177 0.0 7 0.0 0.0 0.0 0.023818197102586376 0.0 8 0.0 0.0 0.0 0.023818197102586376 0.0 9 0.0 0.0 0.0 0.025219267520385575 0.0 10 0.0 0.0 0.0 0.025219267520385575 0.0 11 0.0 0.0 0.0 0.025219267520385575 0.0 12 0.0 0.0 0.0 0.025219267520385575 0.0 13 0.0 0.0 0.0 0.026620337938184774 0.0 14 0.0 0.0 0.0 0.026620337938184774 0.0 15 0.0 0.0 0.0 0.026620337938184774 0.0 16 0.0 0.0 0.0 0.02942247877378317 0.0 17 0.0 0.0 0.0 0.02942247877378317 0.0 18 0.0 0.0 0.0 0.033625690027180764 0.0 19 0.0 0.0 0.0 0.033625690027180764 0.0 20 0.0 0.0 0.0 0.033625690027180764 0.0 21 0.0 0.0 0.0 0.03502676044497997 0.0 22 0.0 0.0 0.0 0.04203211253397596 0.0 23 0.0 0.0 0.0 0.04343318295177516 0.0 24 0.0 0.0 0.0 0.056042816711967945 0.0 25 0.0 0.0 0.0 0.06164709838316474 0.0 26 0.0 0.0 0.0 0.06865245047216073 0.0 27 0.0 0.0 0.0 0.1064813517527391 0.0 28 0.0 0.0 0.0 0.16672737971810464 0.0 29 0.0 0.0 0.0 0.21856698517667497 0.0 30 0.0 0.0 0.0 0.31524084400481966 0.0 31 0.0 0.0 0.0 0.395101857819374 0.0 32 0.0 0.0 0.0 0.4945778574831171 0.0 33 0.0 0.0 0.0 0.6318827584274386 0.0 34 0.0 0.0 0.0 0.7958079973099448 0.0 35 0.0 0.0 0.0 1.0171771233222182 0.0 36 0.0 0.0 0.0 1.3604393756830218 0.0 37 0.0 0.0 0.0 1.8017765572897695 0.0 38 0.0 0.0 0.0 2.2529212318211114 0.0 39 0.0 0.0 0.0 2.751702300557626 0.0 40 0.0 0.0 0.0 3.2546865805475385 0.0 41 0.0 0.0 0.0 3.81091153641382 0.0 42 0.0 0.0 0.0 4.452601787765853 0.0 43 0.0 0.0 0.0 5.141928433323058 0.0 44 0.0 0.0 0.0 5.768206910079301 0.0 45 0.0 0.0 0.0 6.422506795191526 0.0 46 0.0 0.0 0.0 7.002549948160395 0.0 47 0.0 0.0 0.0 7.627427354498837 0.0 48 0.0 0.0 0.0 8.168240535769328 0.0 49 0.0 0.0 0.0 8.769299745005185 0.0 50 0.0 0.0 0.0 9.289096870008686 0.0 51 0.0 0.0 0.0 9.820102558354582 0.0 52 0.0 0.0 0.0 10.503824922240591 0.0 53 0.0 0.0 0.0 11.193151567797797 0.0 54 0.0 0.0 0.0 11.792809706615854 0.0 55 0.0 0.0 0.0 12.363045366660128 0.0 56 0.0 0.0 0.0 12.9374842379578 0.0 57 0.0 0.0 0.0 13.455880292543503 0.0 58 0.0 0.0 0.0 14.086361980553143 0.0 59 0.0 0.0 0.0 14.57953876761846 0.0 60 0.0 0.0 0.0 15.075517695519377 0.0 61 0.0 0.0 0.0 15.668170482248438 0.0 62 0.0 0.0 0.0 16.244010423963907 0.0 63 0.0 0.0 0.0 16.797433238994593 0.0 64 0.0 0.0 0.0 17.390086025723654 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCATA 20 0.0069254762 52.5 20 TACGACA 20 0.0069254762 52.5 49 GGCATAA 20 0.0069254762 52.5 21 GATCTCG 20 0.0069254762 52.5 41 AGATCTC 30 5.8422016E-4 46.666668 40 GTGTAGA 55 6.6113553E-6 38.18182 36 TCTCGGT 50 1.6944873E-4 35.000004 43 TGTAGAT 50 1.6944873E-4 35.000004 37 TAGATCT 40 0.0024043082 35.0 39 TATACAA 40 0.0024043082 35.0 10 TATGAGG 45 0.004281691 31.111113 16 GCATAAT 50 0.0071658394 28.000002 22 CGGTGGT 50 0.0071658394 28.000002 46 AGGCAAC 175 0.0010016086 14.000001 70 GGCAACC 150 0.0058635217 14.0 70 GAGGCAA 205 2.1180467E-4 13.658537 70 GACGAGG 365 8.312782E-9 13.424657 70 CGACGAG 390 2.102206E-8 12.564102 69 CGAGGCA 230 5.598995E-4 12.173913 69 CCGACGA 505 7.4859236E-7 9.7029705 68 >>END_MODULE