FastQCFastQC Report
Wed 25 May 2016
SRR1780200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71374
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT30584.284473337629949No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG17072.391627203183232Illumina PCR Primer Index 8 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG7911.108246700479166No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5890.8252304760837279No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3300.46235323787373556No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2040.28581836523103654RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT1060.14851346428671505No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT950.13310168969092387No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT780.1092834925883375No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC770.1078824221705383No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG730.1022781404993415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAT150.002216338270.0000120
ATCGTAC150.002216338270.0000125
CACTAGA150.002216338270.0000117
TCACTAG150.002216338270.0000116
CATCGTA150.002216338270.0000124
ACGTACT150.002216338270.0000140
TCTTTAT308.3001505E-658.3333361
CTTTATA308.3001505E-658.3333362
ATTTATA200.006925476252.52
CTAGACA200.006925476252.519
TTAGCAA200.006925476252.54
GCAAACT200.006925476252.57
GTCTAAT200.006925476252.557
ATCACTA200.006925476252.515
CCGTATT200.006925476252.512
ACTAGAC200.006925476252.518
TCCGTAT200.006925476252.511
ACTCGCA200.006925476252.519
GTATCAA200.006925476252.532
TCGTACT200.006925476252.526
Produced by FastQC (version 0.11.5)