##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780200_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71374 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.39255190965898 34.0 34.0 34.0 33.0 34.0 2 33.52797937624345 34.0 34.0 34.0 34.0 34.0 3 33.58391010732199 34.0 34.0 34.0 34.0 34.0 4 33.63331185025359 34.0 34.0 34.0 34.0 34.0 5 33.587048505057865 34.0 34.0 34.0 34.0 34.0 6 37.16116513015944 38.0 38.0 38.0 36.0 38.0 7 37.15565892341749 38.0 38.0 38.0 36.0 38.0 8 36.96029366435957 38.0 37.0 38.0 35.0 38.0 9 37.03782890128058 38.0 37.0 38.0 35.0 38.0 10-11 36.9635231316726 38.0 37.0 38.0 35.0 38.0 12-13 36.93363829966094 38.0 37.0 38.0 35.0 38.0 14-15 36.82232325496679 38.0 37.0 38.0 34.0 38.0 16-17 36.871178580435455 38.0 37.0 38.0 34.5 38.0 18-19 36.831605346484714 38.0 37.0 38.0 34.5 38.0 20-21 36.85983691540336 38.0 37.0 38.0 34.5 38.0 22-23 36.81510774231513 38.0 37.0 38.0 34.5 38.0 24-25 36.82540560988595 38.0 37.0 38.0 34.0 38.0 26-27 36.64877266231401 38.0 37.0 38.0 34.0 38.0 28-29 36.60577381119175 38.0 37.0 38.0 34.0 38.0 30-31 36.55885896825174 38.0 37.0 38.0 34.0 38.0 32-33 36.43891332978396 38.0 37.0 38.0 34.0 38.0 34-35 36.47871774035363 38.0 37.0 38.0 34.0 38.0 36-37 36.56886961638692 38.0 37.0 38.0 34.0 38.0 38-39 36.022199960770024 38.0 37.0 38.0 33.5 38.0 40-41 36.23804886933617 38.0 37.0 38.0 34.0 38.0 42-43 36.42107770336537 38.0 37.0 38.0 34.0 38.0 44-45 36.5050928909687 38.0 37.0 38.0 34.0 38.0 46-47 36.403115980609186 38.0 37.0 38.0 34.0 38.0 48-49 36.441722475971645 38.0 37.0 38.0 34.0 38.0 50-51 36.19778911088072 38.0 37.0 38.0 34.0 38.0 52-53 36.24812957099223 38.0 37.0 38.0 34.0 38.0 54-55 36.16168352621403 38.0 37.0 38.0 33.5 38.0 56-57 36.242679407067 38.0 37.0 38.0 34.0 38.0 58-59 36.107069801328215 38.0 37.0 38.0 33.5 38.0 60-61 36.12376355535629 38.0 37.0 38.0 34.0 38.0 62-63 36.0427816852075 38.0 37.0 38.0 33.0 38.0 64-65 35.947733068064 38.0 37.0 38.0 32.5 38.0 66-67 35.471102922632895 38.0 36.5 38.0 29.5 38.0 68-69 35.406583629893234 38.0 36.0 38.0 30.5 38.0 70-71 34.83558438647127 38.0 35.5 38.0 25.5 38.0 72-73 34.10214503880965 38.0 34.5 38.0 22.5 38.0 74-75 33.836263905623895 38.0 34.0 38.0 21.5 38.0 76 32.95345644072071 37.0 33.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06513305026574301 1101 2 0.04747384889127204 1101 3 -0.03886375369287265 1101 4 0.028773951418344268 1101 5 -0.02159321553298099 1101 6 -0.11186242374125754 1101 7 0.2745371552124496 1101 8 0.1002323873629436 1101 9 -0.048102610051060424 1101 10-11 0.13873641384878255 1101 12-13 0.21817391172699985 1101 14-15 0.34879949633227625 1101 16-17 0.2264384280348608 1101 18-19 0.3590499482710783 1101 20-21 0.17173244991175807 1101 22-23 0.09508684769668463 1101 24-25 0.31967795601666893 1101 26-27 0.19694517740972373 1101 28-29 -0.20873850777556413 1101 30-31 0.139085787466513 1101 32-33 0.005656894150462222 1101 34-35 0.5542979807571484 1101 36-37 0.45739926926592744 1101 38-39 0.2450506536638244 1101 40-41 0.17180273784150302 1101 42-43 0.40305654596725304 1101 44-45 -0.05200692347528957 1101 46-47 0.17876474234179796 1101 48-49 0.5219897994714628 1101 50-51 0.04709319922947941 1101 52-53 0.13935094312380158 1101 54-55 -0.14753978451956584 1101 56-57 0.09834251422859097 1101 58-59 0.2865142179547604 1101 60-61 0.5923156180295877 1101 62-63 0.4210489204666956 1101 64-65 0.17141451066571278 1101 66-67 0.35033877651070355 1101 68-69 -0.33814551671891024 1101 70-71 0.0897837075686283 1101 72-73 -0.4308815625609981 1101 74-75 -0.48602858187663145 1101 76 -0.8564622055643838 1102 1 0.08795913722226345 1102 2 0.24680764973278713 1102 3 -0.23027329085977044 1102 4 -0.32999182698277707 1102 5 0.020798088814842686 1102 6 0.3105779689656174 1102 7 -0.02837308321672083 1102 8 0.06292523448776421 1102 9 0.027633715334630438 1102 10-11 0.3283051375514461 1102 12-13 0.2992748934941858 1102 14-15 0.4511311933869777 1102 16-17 0.3169713873616544 1102 18-19 0.11199524981385167 1102 20-21 0.4669989295751549 1102 22-23 0.22196272427451902 1102 24-25 0.11187641323967767 1102 26-27 0.47941362060747394 1102 28-29 0.43161913598320467 1102 30-31 0.43738522645669775 1102 32-33 0.606445814206559 1102 34-35 0.18333023882166088 1102 36-37 0.15359492143984 1102 38-39 0.3476803871841625 1102 40-41 0.6516239159621264 1102 42-43 0.6385930116054013 1102 44-45 0.5372462322049358 1102 46-47 0.46328437768541875 1102 48-49 0.23608517114046634 1102 50-51 0.4567706185843221 1102 52-53 0.2833025560270386 1102 54-55 0.3787049560133937 1102 56-57 0.2860879560238203 1102 58-59 0.44328139888042273 1102 60-61 0.5826907933451579 1102 62-63 0.29934485314551296 1102 64-65 0.4919439636811447 1102 66-67 0.4531613571558708 1102 68-69 0.363362197166083 1102 70-71 0.6627500469795677 1102 72-73 0.984984496345028 1102 74-75 0.6869202259775022 1102 76 0.8347369529208919 1103 1 0.30405225463117347 1103 2 0.2014080154112463 1103 3 0.13140556508903245 1103 4 -0.021058824501388074 1103 5 0.12859161108205086 1103 6 -0.12085731900617702 1103 7 0.10560562907268434 1103 8 0.3464566436558485 1103 9 0.2423392135715403 1103 10-11 0.2281924955245458 1103 12-13 0.08758950757280104 1103 14-15 0.12015137100222972 1103 16-17 0.0563275320658434 1103 18-19 0.3422747326400284 1103 20-21 0.12210210314182746 1103 22-23 0.34135511032650356 1103 24-25 -0.1941752388433784 1103 26-27 -0.15575681167671007 1103 28-29 0.19685029701232537 1103 30-31 -0.10110079940199768 1103 32-33 0.25417828123020314 1103 34-35 -0.43962914474892756 1103 36-37 -0.009613580584449721 1103 38-39 -0.20137603177887797 1103 40-41 0.41020266743136347 1103 42-43 -0.07590842498856176 1103 44-45 0.16893129162768616 1103 46-47 0.003109047921803665 1103 48-49 0.012009162259701611 1103 50-51 -0.30553837971788766 1103 52-53 0.0484602548646933 1103 54-55 0.004202866422161833 1103 56-57 -0.45686783606184633 1103 58-59 0.3058418011267321 1103 60-61 0.356468055980649 1103 62-63 0.48850183368620037 1103 64-65 0.38606862085758564 1103 66-67 0.260257804850788 1103 68-69 0.35035122681216535 1103 70-71 0.13243464930423698 1103 72-73 0.6210283124610001 1103 74-75 0.606560750987299 1103 76 0.19844010036766946 1104 1 0.2946324439951695 1104 2 -0.10521972464069052 1104 3 0.11632419018379636 1104 4 0.16036930911798208 1104 5 -0.16993896297798727 1104 6 0.09820166771898897 1104 7 0.046215658590242015 1104 8 -0.2960260571199953 1104 9 0.06708879822885905 1104 10-11 0.010310985128882066 1104 12-13 0.09353334356486442 1104 14-15 0.24497999174194263 1104 16-17 0.1521789442309469 1104 18-19 -0.11763461781241347 1104 20-21 0.17045928171498304 1104 22-23 0.2300513375633031 1104 24-25 0.0691842785526191 1104 26-27 0.03583310396437156 1104 28-29 0.242231525136269 1104 30-31 0.2641507450275711 1104 32-33 0.33963402908896967 1104 34-35 0.7694374229882257 1104 36-37 0.24896344733625853 1104 38-39 0.03234495472047172 1104 40-41 0.09741333891546589 1104 42-43 0.10849565908002035 1104 44-45 0.06083735159832315 1104 46-47 0.12459879742581137 1104 48-49 -0.08646334447449533 1104 50-51 -0.3345801075434238 1104 52-53 0.147916680175598 1104 54-55 -0.2866095922451848 1104 56-57 0.06310087210145099 1104 58-59 0.43468674523693096 1104 60-61 0.5812208666005247 1104 62-63 0.7329491456529098 1104 64-65 0.2965400952837598 1104 66-67 -0.06499986890762699 1104 68-69 -0.15798788637073358 1104 70-71 -0.012693340759803107 1104 72-73 0.8403292463771592 1104 74-75 0.7388977995541666 1104 76 0.7889041550799973 1105 1 -0.2705119371578988 1105 2 0.2001867486397444 1105 3 -0.05255401599973197 1105 4 -0.02487319195211768 1105 5 0.056955113608232466 1105 6 0.0651767426259724 1105 7 0.06483539452254661 1105 8 0.27033659145924105 1105 9 0.21025168387635063 1105 10-11 0.33298722232884614 1105 12-13 0.2363916623558211 1105 14-15 -0.028257636258452123 1105 16-17 0.37162348013691116 1105 18-19 0.28668200001380484 1105 20-21 0.29907704207848695 1105 22-23 0.37253923720440696 1105 24-25 0.3512625769293649 1105 26-27 0.26320460967706083 1105 28-29 0.38026616344254904 1105 30-31 0.23268981190057048 1105 32-33 0.4863611701116568 1105 34-35 0.03363682373235832 1105 36-37 0.21609492143984 1105 38-39 0.07825223469149023 1105 40-41 0.2826723030588951 1105 42-43 0.3208610734555748 1105 44-45 0.3023588688351566 1105 46-47 0.09146686235617096 1105 48-49 -0.024578651840073462 1105 50-51 -0.24051010655564653 1105 52-53 0.5393509431238073 1105 54-55 0.16377893628532547 1105 56-57 0.4697223201933838 1105 58-59 0.390862044041711 1105 60-61 0.4163184926253436 1105 62-63 0.4536037174483738 1105 64-65 0.1080296741583453 1105 66-67 0.40612390047764535 1105 68-69 0.7836834448333718 1105 70-71 0.869661537248831 1105 72-73 0.8222445603279667 1105 74-75 0.48405765599616757 1105 76 0.5827041616652266 1106 1 0.35081921952679096 1106 2 0.09837740945666695 1106 3 0.08937027743971271 1106 4 -0.019159489268716356 1106 5 0.21472812996710644 1106 6 0.08085541487029957 1106 7 0.1422594668961139 1106 8 0.3969223140045557 1106 9 0.22582832571562506 1106 10-11 0.21662154535710698 1106 12-13 0.41066809673326077 1106 14-15 0.3636411495797276 1106 16-17 0.16513944789530655 1106 18-19 0.24736628393041116 1106 20-21 0.13388131375144496 1106 22-23 0.28965653816009507 1106 24-25 0.25395958063646873 1106 26-27 0.1278272672290086 1106 28-29 0.4071766827773118 1106 30-31 0.3673019263106738 1106 32-33 0.4395717268575652 1106 34-35 0.21273419474891142 1106 36-37 0.39819368687194157 1106 38-39 -0.07653639231935472 1106 40-41 0.04193156231815465 1106 42-43 0.06077706842207675 1106 44-45 0.20445219264566106 1106 46-47 0.23564432724070628 1106 48-49 -0.005618001566297437 1106 50-51 0.4528545161019082 1106 52-53 0.6741246056752459 1106 54-55 0.41775812208268803 1106 56-57 0.6253867081809972 1106 58-59 0.4381871469223739 1106 60-61 0.7415999264926398 1106 62-63 0.6907393838769664 1106 64-65 0.7048820361622248 1106 66-67 0.7032194349469165 1106 68-69 0.7231624095648641 1106 70-71 0.1074433962428003 1106 72-73 -0.07668582092564691 1106 74-75 -0.01984500699740721 1106 76 -0.39911742116645854 1107 1 -0.1636366074585851 1107 2 -0.09692948135850088 1107 3 0.013772694040710576 1107 4 -0.18112429094983185 1107 5 -0.15739340054685869 1107 6 -0.26958675306595836 1107 7 0.01079987491642953 1107 8 0.013645893375894502 1107 9 0.28899267209509816 1107 10-11 -0.07578717542040891 1107 12-13 0.27492691820248183 1107 14-15 0.3605941309298757 1107 16-17 0.11940790074531549 1107 18-19 0.23509065132380869 1107 20-21 0.3416214415861276 1107 22-23 0.16821358220177274 1107 24-25 0.39812383945793073 1107 26-27 0.35799975650314764 1107 28-29 0.25569257455561 1107 30-31 0.39236978376622744 1107 32-33 0.26546262958061817 1107 34-35 0.23302138501247072 1107 36-37 0.21503109165260526 1107 38-39 0.11355204126789431 1107 40-41 0.11245953710145074 1107 42-43 0.11619253116614203 1107 44-45 0.5067812356366375 1107 46-47 0.37978231866001977 1107 48-49 0.2700934072420438 1107 50-51 0.4449655396550156 1107 52-53 0.4553083899323198 1107 54-55 0.3402955531307583 1107 56-57 -0.0017037392404120055 1107 58-59 0.1270322568076665 1107 60-61 -0.40199520528216226 1107 62-63 0.2648047021503217 1107 64-65 0.2601749176962471 1107 66-67 0.9131047339575176 1107 68-69 1.047284640542884 1107 70-71 0.27618518767962996 1107 72-73 0.27577893697646516 1107 74-75 0.32081693153687496 1107 76 0.43835740590647987 1108 1 0.10466420634669049 1108 2 0.04116248844245973 1108 3 -0.02117190837129357 1108 4 0.12853351863934392 1108 5 0.06400085379180354 1108 6 -0.3414957637532794 1108 7 0.16920756194456743 1108 8 0.10900818379651867 1108 9 -0.018449233974124013 1108 10-11 -0.1811994707434792 1108 12-13 0.07404447874763775 1108 14-15 0.1685851104376681 1108 16-17 0.011671848191141976 1108 18-19 0.29978326824703316 1108 20-21 0.373104920358557 1108 22-23 0.25738899155562933 1108 24-25 0.17207226577423995 1108 26-27 0.08310025655217146 1108 28-29 0.15224125579887016 1108 30-31 0.0517285444290394 1108 32-33 0.19947576812361234 1108 34-35 0.17180950149447227 1108 36-37 0.42231611959652327 1108 38-39 0.49226564064038314 1108 40-41 0.09373221089299477 1108 42-43 -0.14683325567570193 1108 44-45 0.37278770685931306 1108 46-47 0.40078437768541875 1108 48-49 0.2487579822003667 1108 50-51 0.08580287664883457 1108 52-53 0.07276108137265425 1108 54-55 -0.04093790112946749 1108 56-57 0.144094868466226 1108 58-59 0.20192195187581063 1108 60-61 0.7569419454188875 1108 62-63 0.41628033701648093 1108 64-65 0.35283106045533685 1108 66-67 0.46900237098075337 1108 68-69 0.47108109117530717 1108 70-71 1.0884412912191976 1108 72-73 1.423636570077747 1108 74-75 0.7476921153784275 1108 76 1.0876171372526855 1109 1 0.06763894017792893 1109 2 -0.14539400289712034 1109 3 0.026182294711496468 1109 4 0.24532995912877453 1109 5 0.2430887292089281 1109 6 0.13617784184023662 1109 7 0.2976041931220692 1109 8 0.16859820445439766 1109 9 0.11360225180372652 1109 10-11 0.1965218068665635 1109 12-13 0.12004797469551676 1109 14-15 0.18097983473450796 1109 16-17 0.1368903449335548 1109 18-19 0.19355412475426448 1109 20-21 0.37818993545469937 1109 22-23 0.25421085712054037 1109 24-25 0.22633891125334316 1109 26-27 0.11774192189461985 1109 28-29 0.12888196770098403 1109 30-31 0.32767006675543087 1109 32-33 0.28647719828411056 1109 34-35 0.06661325169310572 1109 36-37 -0.024052861811391324 1109 38-39 0.2084582355871447 1109 40-41 0.10286934739387021 1109 42-43 0.20911780105399913 1109 44-45 0.6760611853274483 1109 46-47 0.6142755583623654 1109 48-49 0.595174635624808 1109 50-51 0.4342853174560872 1109 52-53 0.2942770514981916 1109 54-55 -0.08972226470804401 1109 56-57 0.55478929084029 1109 58-59 0.3416009602978676 1109 60-61 0.12226849873209034 1109 62-63 0.2081125315183101 1109 64-65 0.06679896129754326 1109 66-67 -0.14842969639570214 1109 68-69 0.288894534683962 1109 70-71 0.23686016933773857 1109 72-73 -0.319808527649883 1109 74-75 -0.12438797360933762 1109 76 -0.25117199352754227 1110 1 0.11982188232030211 1110 2 0.17620360166194615 1110 3 0.047794387824602325 1110 4 0.05963499148654705 1110 5 0.19971965744229436 1110 6 0.09568233329198961 1110 7 -0.13582090105353473 1110 8 0.27086324840301046 1110 9 0.26008153317144433 1110 10-11 0.14191203192210367 1110 12-13 0.38458482391796167 1110 14-15 -0.15959777561997868 1110 16-17 0.12492521405361146 1110 18-19 0.15943357998462915 1110 20-21 0.16631898017604385 1110 22-23 0.0457688596318917 1110 24-25 0.46888937971659317 1110 26-27 0.1067064732323928 1110 28-29 0.044522361789653075 1110 30-31 0.07333557433778282 1110 32-33 0.10917351819139043 1110 34-35 -0.043741234929122186 1110 36-37 0.1670753135967047 1110 38-39 0.008733518113956507 1110 40-41 0.3488487736471271 1110 42-43 0.029761575805274276 1110 44-45 0.29780126066792434 1110 46-47 0.18786533910224534 1110 48-49 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-0.12059535380810615 2116 6 0.006241638981478559 2116 7 0.296917768184656 2116 8 -0.0021197152742686853 2116 9 0.0035723721299802946 2116 10-11 -0.5750198227869916 2116 12-13 -0.2586970790071419 2116 14-15 0.0023276502880875682 2116 16-17 -0.8244881578525209 2116 18-19 -0.6768730738244315 2116 20-21 -0.27381281024504744 2116 22-23 -0.3581205649904149 2116 24-25 -0.3015622435556722 2116 26-27 -0.9449650150350379 2116 28-29 -0.34687108083330287 2116 30-31 -0.23279589464326023 2116 32-33 -0.21544075374689697 2116 34-35 0.062494702068626395 2116 36-37 -0.32898704577327464 2116 38-39 -0.7403021617317549 2116 40-41 -0.5288031067771684 2116 42-43 -0.6746341168980337 2116 44-45 -1.0299470517717992 2116 46-47 -0.9266878592262628 2116 48-49 -0.5155012910488566 2116 50-51 -0.7037264729016641 2116 52-53 -0.4483539749089829 2116 54-55 -0.17255760347893556 2116 56-57 -0.8267822184870113 2116 58-59 -1.1378264805484548 2116 60-61 -0.26160455303242003 2116 62-63 -0.4183309797471395 2116 64-65 -0.7216004572601591 2116 66-67 0.2519781207729963 2116 68-69 0.3516620385198692 2116 70-71 0.13016146950732832 2116 72-73 -0.6096413629886541 2116 74-75 -0.02986322193365254 2116 76 -1.614694564794604 2117 1 -0.4027433421165796 2117 2 -0.19237523483133145 2117 3 -0.0442895531898202 2117 4 -0.06619550600170498 2117 5 -0.00916058887110438 2117 6 0.0073255029565544305 2117 7 -0.2533464236592735 2117 8 -0.4486716730035738 2117 9 -0.7236326136443054 2117 10-11 -0.29924418262983465 2117 12-13 -0.484682717809477 2117 14-15 0.05851922324568193 2117 16-17 -0.18416164383003064 2117 18-19 -0.20918576858124283 2117 20-21 -0.9757163463512626 2117 22-23 0.08741527026225526 2117 24-25 -0.28923329083923477 2117 26-27 0.06898973364400263 2117 28-29 -0.9027916877971265 2117 30-31 0.030210473057600495 2117 32-33 -0.8380189951775989 2117 34-35 -0.21016482916020607 2117 36-37 -0.20332656616346156 2117 38-39 0.07618611898901406 2117 40-41 -0.4115425729741631 2117 42-43 -0.24732074472624532 2117 44-45 -0.7823518749664942 2117 46-47 -0.30316123681738105 2117 48-49 -0.5452398088648707 2117 50-51 -0.42646051977877875 2117 52-53 -0.004037486628263309 2117 54-55 -0.3713450306568262 2117 56-57 -0.8319305723686057 2117 58-59 -0.2826916749665571 2117 60-61 -0.5237641520027481 2117 62-63 -0.25714008420452217 2117 64-65 -0.4064331357590092 2117 66-67 -0.8004876697702912 2117 68-69 -0.7328454807864517 2117 70-71 -0.5642227602718322 2117 72-73 -0.386433125622446 2117 74-75 -0.02214069111126804 2117 76 0.02485292199580158 2118 1 -0.840438921030163 2118 2 -0.29676422624591936 2118 3 -0.7397079917680074 2118 4 -0.325763828235516 2118 5 -0.041095115068650045 2118 6 -0.3190806592836779 2118 7 -0.27645482452583536 2118 8 -0.46447846109632707 2118 9 -0.32501269869606375 2118 10-11 -0.36134800995872496 2118 12-13 -0.33676488101255586 2118 14-15 -0.7580177261244572 2118 16-17 -0.8039963545738331 2118 18-19 -0.8495612800953225 2118 20-21 -0.39202863071297855 2118 22-23 -0.5883449800787446 2118 24-25 -0.584627658166518 2118 26-27 -0.3713536828688788 2118 28-29 -0.39601161879287616 2118 30-31 -0.8138479398445924 2118 32-33 -1.1650269386903958 2118 34-35 -0.3389059027379986 2118 36-37 -0.00963323389996873 2118 38-39 0.027722667171630633 2118 40-41 -0.24159954160129615 2118 42-43 -0.15625117881113226 2118 44-45 -0.05692461026296769 2118 46-47 -0.06758392798323598 2118 48-49 3.350928441889778E-4 2118 50-51 0.1451514516566661 2118 52-53 -0.2203577947402664 2118 54-55 -0.439667264468909 2118 56-57 -0.7912100395915829 2118 58-59 -0.5703030045019801 2118 60-61 -0.5888367669429755 2118 62-63 0.2814541547427609 2118 64-65 -0.5607298540451353 2118 66-67 -0.3899524856252228 2118 68-69 0.4799844959446631 2118 70-71 0.24288628249475153 2118 72-73 -0.5256050745913967 2118 74-75 -0.35263896600495315 2118 76 -0.4659598839096759 2119 1 -0.5241542648395949 2119 2 0.04814619313152235 2119 3 0.1566987743214696 2119 4 -0.2962372543189886 2119 5 -0.6866761379892807 2119 6 -0.6609883762289286 2119 7 -0.5231768743706979 2119 8 -0.18582767538919143 2119 9 -0.6334903285629423 2119 10-11 -1.1191300137621525 2119 12-13 -1.0123729282550755 2119 14-15 -1.0906671105704575 2119 16-17 -0.4019344989037279 2119 18-19 -0.7670370082506608 2119 20-21 -0.4208045290214528 2119 22-23 -0.572080341904396 2119 24-25 -1.7057366562648113 2119 26-27 -0.2108091928278384 2119 28-29 0.0432337019958382 2119 30-31 -1.0978034998485953 2119 32-33 -1.3937828189826647 2119 34-35 -0.8374545965624378 2119 36-37 -0.7761731197972708 2119 38-39 0.12440968548811782 2119 40-41 0.17442488037848136 2119 42-43 -0.6888305002003818 2119 44-45 -0.4786541668639046 2119 46-47 -0.35794525317589887 2119 48-49 -0.5614705322183653 2119 50-51 0.009979797167623872 2119 52-53 -0.8240511187318589 2119 54-55 -0.35123767784094895 2119 56-57 -0.8017695431447862 2119 58-59 -1.0833647600819987 2119 60-61 -0.6073033869009308 2119 62-63 -0.850264391949267 2119 64-65 -0.4908669774562071 2119 66-67 -0.9512076152418771 2119 68-69 -0.8920002903630149 2119 70-71 0.05786976763137375 2119 72-73 0.4697907816792508 2119 74-75 0.29859714649629865 2119 76 -0.46385799220714574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 4.0 22 5.0 23 12.0 24 23.0 25 55.0 26 113.0 27 216.0 28 419.0 29 689.0 30 1008.0 31 1469.0 32 2394.0 33 3787.0 34 6126.0 35 8488.0 36 13126.0 37 33437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.901028385686665 18.54737019082579 13.095805195169108 32.455796228318434 2 19.769103595146692 20.448622747779304 35.07159469834954 24.710678958724465 3 18.595006585030962 20.01989519993275 31.477849076694596 29.90724913834169 4 15.048897357581193 23.294196766329474 32.98119763499314 28.6757082410962 5 15.59671589094068 31.651581808501696 37.625746069997476 15.125956230560147 6 29.489730153837534 36.42082551069017 16.76380754896741 17.325636786504887 7 27.305461372488583 25.94081878555216 23.08823941491299 23.665480427046262 8 23.986325552722278 35.4919158236893 17.10006444923922 23.421694174349202 9 22.921512035194887 16.15574298764256 18.46190489534004 42.46084008182252 10-11 26.883739176731027 26.075321545660884 24.009443214615967 23.031496062992126 12-13 25.88617703925799 19.835654439992155 25.129599013646427 29.148569507103428 14-15 22.777201782161573 19.581360159161598 20.991537534676493 36.64990052400034 16-17 26.391963460083506 25.654299885112223 20.87314708437246 27.080589570431812 18-19 28.3240395662286 24.444475579342615 21.52814750469359 25.7033373497352 20-21 24.697368789755373 23.52887606131084 21.189788998795077 30.583966150138707 22-23 23.6038333286631 26.357637234847424 21.197494886092976 28.841034550396504 24-25 26.646958276122955 26.76604926163589 22.119399221004848 24.467593241236305 26-27 27.95625858155631 27.605990977106508 21.678762574607 22.75898786673018 28-29 23.502255723372656 26.178300221369128 24.919438450976546 25.400005604281674 30-31 22.60767226160787 28.11598060918542 25.43643343514445 23.839913694062265 32-33 24.8283688738196 27.18777145739345 24.252528932104127 23.731330736682825 34-35 24.625914198447614 22.52991285342001 29.573794378905482 23.270378569226892 36-37 24.278448734833415 27.198980020735842 25.712444307450895 22.810126936979852 38-39 22.10118530557346 25.934513968672064 27.037856922688935 24.926443803065542 40-41 23.545688906324433 21.172275618572588 27.231204640345226 28.050830834757758 42-43 26.67778182531454 21.021660548659177 26.405273629052594 25.89528399697369 44-45 22.408720262280383 24.644828649087902 28.502676044498 24.443775044133716 46-47 24.87600526802477 22.11659708016925 27.753803906184327 25.253593745621654 48-49 24.24832572085073 21.29136660408552 25.20875949225208 29.25154818281167 50-51 23.642362765152576 21.64093367332642 26.79687281082747 27.91983075069353 52-53 20.405189564827527 21.744612884243562 33.207470507467704 24.642727043461203 54-55 21.57368229327206 22.839549415753634 28.976938380923023 26.609829910051282 56-57 23.245159301706504 24.204192002690057 25.31243870316922 27.23820999243422 58-59 21.12113654832292 24.45148093143161 29.07431277496007 25.3530697452854 60-61 23.56460335696472 27.146439880068375 25.454647350575836 23.834309412391068 62-63 20.137585115027882 32.44178552414044 22.995768767338244 24.424860593493428 64-65 19.753691820550902 33.85756718132653 21.918345616050665 24.470395382071903 66-67 19.927424552358 33.07506935298568 22.0318323198924 24.965673774763918 68-69 19.47417827219996 33.44915515453807 22.351276375150615 24.72539019811136 70-71 19.7578950318043 32.361223975116985 22.41502507916048 25.465855913918233 72-73 19.9288256227758 30.40252753103371 22.94112702104408 26.727519825146413 74-75 19.71936559531482 30.183960545857037 23.587721018858407 26.508952839969734 76 19.627595482948973 30.40182699582481 23.537983019026537 26.43259450219968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.5 12 3.0 13 1.5 14 0.0 15 1.0 16 1.5 17 1.0 18 2.5 19 6.5 20 9.5 21 10.0 22 19.0 23 36.5 24 53.5 25 62.0 26 94.5 27 151.5 28 227.5 29 279.0 30 361.5 31 469.5 32 641.5 33 788.0 34 895.0 35 1214.0 36 2213.5 37 3001.0 38 3728.5 39 4428.5 40 4278.5 41 3881.0 42 3606.0 43 3593.5 44 3653.0 45 3679.5 46 3634.0 47 3558.0 48 3333.5 49 2951.0 50 2717.0 51 2532.0 52 2074.0 53 1717.5 54 1634.0 55 1627.0 56 1463.0 57 1303.0 58 1300.0 59 1234.5 60 1215.0 61 1316.5 62 1372.0 63 1435.5 64 1544.0 65 1650.5 66 1676.0 67 1640.0 68 1591.0 69 1513.5 70 1373.0 71 1261.0 72 1065.5 73 730.5 74 451.5 75 312.0 76 223.5 77 94.5 78 35.5 79 17.0 80 12.5 81 5.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 71374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.96570179617228 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4340792666487 36.60156359458626 2 15.494742518198976 16.103903382183987 3 5.637638177406309 8.788914730854373 4 2.8686977622000542 5.962955698153389 5 1.7120517659746561 4.448398576512456 6 1.1215961175519007 3.4970717628268 7 0.6740361283364789 2.4518732311485976 8 0.5176597465624158 2.1520441617395694 9 0.3990293879751955 1.8662257965085325 >10 1.1027231059584794 7.91885000140107 >50 0.018873011593421408 0.7019362793173985 >100 0.008088433540037746 0.896685067391487 >500 0.005392289026691831 1.933477176562894 >1k 0.005392289026691831 6.676100540813182 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 3058 4.284473337629949 No Hit CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG 1707 2.391627203183232 Illumina PCR Primer Index 8 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 791 1.108246700479166 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 589 0.8252304760837279 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 330 0.46235323787373556 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 204 0.28581836523103654 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 106 0.14851346428671505 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT 95 0.13310168969092387 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 78 0.1092834925883375 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 77 0.1078824221705383 No Hit CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 73 0.1022781404993415 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002802140835598397 0.0 2 0.0 0.0 0.0 0.0042032112533975955 0.0 3 0.0 0.0 0.0 0.0042032112533975955 0.0 4 0.0 0.0 0.0 0.01821391543138958 0.0 5 0.0 0.0 0.0 0.022417126684787177 0.0 6 0.0 0.0 0.0 0.022417126684787177 0.0 7 0.0 0.0 0.0 0.023818197102586376 0.0 8 0.0 0.0 0.0 0.023818197102586376 0.0 9 0.0 0.0 0.0 0.025219267520385575 0.0 10 0.0 0.0 0.0 0.025219267520385575 0.0 11 0.0 0.0 0.0 0.025219267520385575 0.0 12 0.0 0.0 0.0 0.025219267520385575 0.0 13 0.0 0.0 0.0 0.026620337938184774 0.0 14 0.0 0.0 0.0 0.028021408355983973 0.0 15 0.0 0.0 0.0 0.028021408355983973 0.0 16 0.0 0.0 0.0 0.03082354919158237 0.0 17 0.0 0.0 0.0 0.03082354919158237 0.0 18 0.0 0.0 0.0 0.033625690027180764 0.0 19 0.0 0.0 0.0 0.033625690027180764 0.0 20 0.0 0.0 0.0 0.033625690027180764 0.0 21 0.0 0.0 0.0 0.03502676044497997 0.0 22 0.0 0.0 0.0 0.04063104211617676 0.0 23 0.0 0.0 0.0 0.04203211253397596 0.0 24 0.0 0.0 0.0 0.04903746462297195 0.0 25 0.0 0.0 0.0 0.05464174629416874 0.0 26 0.0 0.0 0.0 0.06164709838316474 0.0 27 0.0 0.0 0.0 0.0994759996637431 0.0 28 0.0 0.0 0.0 0.15691988679351024 0.0 29 0.0 0.0 0.0 0.2059573514164822 0.0 30 0.0 0.0 0.0 0.2970269285734301 0.0 31 0.0 0.0 0.0 0.37268473113458683 0.0 32 0.0 0.0 0.0 0.4679575195449323 0.0 33 0.0 0.0 0.0 0.6024602796536553 0.0 34 0.0 0.0 0.0 0.76918765937176 0.0 35 0.0 0.0 0.0 0.9849525037128366 0.0 36 0.0 0.0 0.0 1.3282147560736404 0.0 37 0.0 0.0 0.0 1.756942303920195 0.0 38 0.0 0.0 0.0 2.1912741334379464 0.0 39 0.0 0.0 0.0 2.6620337938184773 0.0 40 0.0 0.0 0.0 3.1369966654524055 0.0 41 0.0 0.0 0.0 3.669403424216101 0.0 42 0.0 0.0 0.0 4.276066915123154 0.0 43 0.0 0.0 0.0 4.945778574831171 0.0 44 0.0 0.0 0.0 5.552442065738224 0.0 45 0.0 0.0 0.0 6.173116260823269 0.0 46 0.0 0.0 0.0 6.741950850449744 0.0 47 0.0 0.0 0.0 7.355619693445792 0.0 48 0.0 0.0 0.0 7.869812536778099 0.0 49 0.0 0.0 0.0 8.437246055986774 0.0 50 0.0 0.0 0.0 8.947235688065682 0.0 51 0.0 0.0 0.0 9.465631742651386 0.0 52 0.0 0.0 0.0 10.131140191106004 0.0 53 0.0 0.0 0.0 10.806456132485218 0.0 54 0.0 0.0 0.0 11.386499285454088 0.0 55 0.0 0.0 0.0 11.952531734244964 0.0 56 0.0 0.0 0.0 12.511558830946843 0.0 57 0.0 0.0 0.0 13.011740970101158 0.0 58 0.0 0.0 0.0 13.611399108919214 0.0 59 0.0 0.0 0.0 14.083559839717545 0.0 60 0.0 0.0 0.0 14.566929133858268 0.0 61 0.0 0.0 0.0 15.142769075573739 0.0 62 0.0 0.0 0.0 15.707400453946816 0.0 63 0.0 0.0 0.0 16.242609353546108 0.0 64 0.0 0.0 0.0 16.808641802336986 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAT 15 0.0022163382 70.00001 20 ATCGTAC 15 0.0022163382 70.00001 25 CACTAGA 15 0.0022163382 70.00001 17 TCACTAG 15 0.0022163382 70.00001 16 CATCGTA 15 0.0022163382 70.00001 24 ACGTACT 15 0.0022163382 70.00001 40 TCTTTAT 30 8.3001505E-6 58.333336 1 CTTTATA 30 8.3001505E-6 58.333336 2 ATTTATA 20 0.0069254762 52.5 2 CTAGACA 20 0.0069254762 52.5 19 TTAGCAA 20 0.0069254762 52.5 4 GCAAACT 20 0.0069254762 52.5 7 GTCTAAT 20 0.0069254762 52.5 57 ATCACTA 20 0.0069254762 52.5 15 CCGTATT 20 0.0069254762 52.5 12 ACTAGAC 20 0.0069254762 52.5 18 TCCGTAT 20 0.0069254762 52.5 11 ACTCGCA 20 0.0069254762 52.5 19 GTATCAA 20 0.0069254762 52.5 32 TCGTACT 20 0.0069254762 52.5 26 >>END_MODULE