FastQCFastQC Report
Wed 25 May 2016
SRR1780199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592309
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACAC553.450623E-935.966233
CCGTAAT353.233646E-431.3990861
GACGAAT300.00574294429.3330523
CCCTAGT406.9832447E-427.52997615
CGAAACT500.002580305621.9979325
GTACAGA602.882069E-421.98678632
TATCAGG500.002592991321.979362
GTATCAG500.002592991321.979361
CTCTAGC602.8886602E-421.979362
CCAAAGT654.928391E-420.30749923
TAGCCCG654.9593666E-420.2886415
ATAGTAT654.9593666E-420.2886416
GTATTAG1553.45608E-1119.8523241
CCTAGTA708.0573856E-418.87769916
CAAAGTG708.084926E-418.86812224
CTGCCGA4650.018.47172518
TAGATTA600.00738291418.34400735
CTAGTCC600.00744796218.3161333
TACTCAC600.00744796218.3161332
CGTGATA750.001290628317.59834529
Produced by FastQC (version 0.11.5)