FastQCFastQC Report
Wed 25 May 2016
SRR1780199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592309
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT142362.403475213106672No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC39760.6712712452453027No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG36460.6155570825363113No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG33870.5718299063495574Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC9410.15886977911866948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1350.035.9362531
TACGATG250.002352004635.1994621
TTAAGCC250.00235297935.19648730
TCTTCTG5500.033.59948344
GTCTTCT6100.030.29461943
CTTTATA1650.029.3378372
TTTGAAC300.00574547929.33040629
CCGTCTT6350.029.10191341
ATGCCGT23800.028.65502244
CGTCTTC6700.027.58166742
TTTATAC2650.024.9094833
TATGCCG27600.024.70976643
GTTCTGA450.001378358124.5019911
TTAATAC450.001396123124.4481962
GCCGTCT7650.023.86891440
CTTAATA500.002543922722.0517881
GAATAGA500.002543922722.0517881
GATTTCT703.212165E-521.99966243
AAAACGT703.214259E-521.99780514
AAACGTA703.214259E-521.99780515
Produced by FastQC (version 0.11.5)