FastQCFastQC Report
Wed 25 May 2016
SRR1780198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70373
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAA150.002216208770.02
GAAGCGG150.002216208770.011
AATTTCC200.00692507552.516
AGGGTAA200.00692507552.512
CCTCGAT200.00692507552.536
CGGATGC200.00692507552.534
GGACCAC200.00692507552.516
GACTACA200.00692507552.540
TCTTACG350.001247751440.011
TTACGCT350.001247751440.013
CTTACGC350.001247751440.012
AACAAGC400.002404079835.032
CGTATCA501.6942459E-435.053
TATACCC501.6942459E-435.03
GCCGTAT400.002404079835.051
ATCTTAC652.0762494E-532.30769310
ACATCTT652.0762494E-532.3076938
CGACGAA450.004281286631.11110919
GTATCAT604.937447E-429.16666654
TTCTACA500.00716516727.9999982