##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70373 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.149617040626378 34.0 31.0 34.0 12.0 34.0 2 30.23537436232646 34.0 32.0 34.0 12.0 34.0 3 30.04007218677618 34.0 31.0 34.0 11.0 34.0 4 29.997527460815938 34.0 31.0 34.0 11.0 34.0 5 29.962698762309408 34.0 31.0 34.0 11.0 34.0 6 32.85028348940645 37.0 34.0 38.0 11.0 38.0 7 33.25417418612252 38.0 34.0 38.0 12.0 38.0 8 33.317067625367685 38.0 34.0 38.0 11.0 38.0 9 33.454790899919004 38.0 34.0 38.0 12.0 38.0 10-11 33.19350461114348 38.0 34.0 38.0 11.0 38.0 12-13 33.232027908430794 38.0 34.0 38.0 11.0 38.0 14-15 33.10651101985137 38.0 34.0 38.0 11.0 38.0 16-17 33.19169994173902 38.0 34.0 38.0 11.0 38.0 18-19 33.188353487843344 38.0 34.0 38.0 11.0 38.0 20-21 33.19836442953974 38.0 34.0 38.0 11.0 38.0 22-23 33.210499765535076 38.0 34.0 38.0 11.0 38.0 24-25 33.22652864024555 38.0 34.0 38.0 11.0 38.0 26-27 33.18742983814815 38.0 34.0 38.0 11.0 38.0 28-29 33.27126881048129 38.0 34.0 38.0 11.0 38.0 30-31 33.2501669674449 38.0 34.0 38.0 11.0 38.0 32-33 33.3216645588507 38.0 34.0 38.0 11.0 38.0 34-35 33.279134042885765 38.0 34.0 38.0 11.0 38.0 36-37 33.2546075909795 38.0 34.0 38.0 11.0 38.0 38-39 33.23811689142143 38.0 34.0 38.0 11.0 38.0 40-41 33.27032384579313 38.0 34.0 38.0 11.0 38.0 42-43 33.36796072357296 38.0 34.0 38.0 11.0 38.0 44-45 33.41094595938783 38.0 34.0 38.0 11.0 38.0 46-47 33.3292384863513 38.0 34.0 38.0 11.0 38.0 48-49 33.272412715103805 38.0 34.0 38.0 11.0 38.0 50-51 33.211863925084906 38.0 34.0 38.0 11.0 38.0 52-53 33.26454037770168 38.0 34.0 38.0 11.0 38.0 54-55 33.26034132408736 38.0 34.0 38.0 11.0 38.0 56-57 33.18882952268626 38.0 34.0 38.0 11.0 38.0 58-59 33.261847583590296 38.0 34.0 38.0 11.0 38.0 60-61 33.292832478365284 38.0 34.0 38.0 11.0 38.0 62-63 33.14797578616799 38.0 34.0 38.0 11.0 38.0 64-65 33.08082645332727 38.0 34.0 38.0 11.0 38.0 66-67 33.0763503048044 38.0 34.0 38.0 11.0 38.0 68-69 33.040796896537024 38.0 34.0 38.0 11.0 38.0 70-71 32.929497108265956 38.0 33.5 38.0 11.0 38.0 72-73 32.99547411649354 38.0 34.0 38.0 11.0 38.0 74-75 33.074737470336636 38.0 34.0 38.0 11.0 38.0 76 31.775922583945547 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5952709439821966 1101 2 -0.06507508390381389 1101 3 0.3369988121708687 1101 4 -0.5442256952950189 1101 5 -0.73277867929529 1101 6 -0.16642789728130936 1101 7 -0.6568932346040768 1101 8 0.3780060507178078 1101 9 -0.014959771792639742 1101 10-11 -0.4520311102532091 1101 12-13 -0.7869100859964107 1101 14-15 -0.5154892577146342 1101 16-17 -0.3796733512039978 1101 18-19 -1.0112432704629768 1101 20-21 -0.23085069742470665 1101 22-23 -0.6112192617113266 1101 24-25 -0.6452414535509092 1101 26-27 -0.4617540453143363 1101 28-29 -0.7028062741082266 1101 30-31 -0.7596321471312208 1101 32-33 -0.4367253152227306 1101 34-35 -1.175625344792202 1101 36-37 -0.9977713128976902 1101 38-39 -0.4685509763275917 1101 40-41 -0.6831372627142756 1101 42-43 -0.8783239273303138 1101 44-45 -0.4169181308792389 1101 46-47 -0.3525370378836854 1101 48-49 -0.4933596100851432 1101 50-51 -0.8881534025796896 1101 52-53 -0.6938051728627457 1101 54-55 -0.757013610947439 1101 56-57 -0.6611035755292676 1101 58-59 -0.5497610446492516 1101 60-61 -0.8758616829115695 1101 62-63 -0.7586601804429165 1101 64-65 -0.47942075219435054 1101 66-67 -0.9633361388489803 1101 68-69 -0.5982316887547938 1101 70-71 -0.42461308108654094 1101 72-73 -0.5678897843670043 1101 74-75 -0.7945217562947136 1101 76 -1.6356425400524408 1102 1 0.20067685326345952 1102 2 -0.3988410367482871 1102 3 0.31529573336201366 1102 4 0.48677870300334547 1102 5 -0.5537463049352063 1102 6 -0.15755919730293755 1102 7 0.41881517984541006 1102 8 0.09103510832830608 1102 9 0.7963460180984896 1102 10-11 0.6552585285094992 1102 12-13 0.5683426359013524 1102 14-15 0.3883610559345172 1102 16-17 0.751374309045481 1102 18-19 0.22929317773083113 1102 20-21 0.42752409527844293 1102 22-23 -0.004975120100539243 1102 24-25 -0.11600358492303542 1102 26-27 -0.11681208843207713 1102 28-29 -0.21286467774223183 1102 30-31 0.41038732074677853 1102 32-33 0.19446655153046777 1102 34-35 0.34438330759802227 1102 36-37 0.5549001125836028 1102 38-39 0.2504265995610808 1102 40-41 0.3978346891207494 1102 42-43 -0.005261702223961606 1102 44-45 0.3545145607351543 1102 46-47 -0.24586027676175348 1102 48-49 0.20373462886503546 1102 50-51 0.4119403316477275 1102 52-53 0.5462986558199319 1102 54-55 0.09373265770928185 1102 56-57 0.17796196632667005 1102 58-59 0.17004571863577667 1102 60-61 0.1369726959189208 1102 62-63 0.04806705295531799 1102 64-65 0.19600645957290652 1102 66-67 0.42108234842479675 1102 68-69 0.08541529372411816 1102 70-71 0.3631149454474567 1102 72-73 0.07375705390540332 1102 74-75 -0.1505315623369654 1102 76 -0.005027499314103068 1103 1 -1.0744658784010106 1103 2 -0.8087883358260193 1103 3 -1.404181649158847 1103 4 -0.25487698430451644 1103 5 -1.0197981275087677 1103 6 -0.9225789601487904 1103 7 -0.7828317239754057 1103 8 -1.2997641889927252 1103 9 -0.7688494144882512 1103 10-11 -0.8490234214246257 1103 12-13 -0.2594790593116514 1103 14-15 -0.9015928307585099 1103 16-17 -1.2827714968394588 1103 18-19 -0.5884933835339865 1103 20-21 -0.6388900461356926 1103 22-23 -0.7033721337149501 1103 24-25 -0.5706258949801253 1103 26-27 -0.9604989571189435 1103 28-29 -0.709669684329846 1103 30-31 -0.5166877780674497 1103 32-33 -0.6280301546618929 1103 34-35 -0.9951753221779995 1103 36-37 -1.0874253156113909 1103 38-39 -1.4501882445320184 1103 40-41 -1.253241288042183 1103 42-43 -1.316768070252067 1103 44-45 -1.3949145126069666 1103 46-47 -1.2434009003893394 1103 48-49 -0.8140629117585902 1103 50-51 -1.431889268703614 1103 52-53 -1.128404901492324 1103 54-55 -1.1699037059270836 1103 56-57 -1.1292514724085052 1103 58-59 -1.4535489234371255 1103 60-61 -0.8785301587143692 1103 62-63 -1.0023282039616817 1103 64-65 -0.9400607341029854 1103 66-67 -0.8796862066915807 1103 68-69 -0.9851380659596813 1103 70-71 -0.9881476808151675 1103 72-73 -1.2613768942720256 1103 74-75 -0.9351933980857581 1103 76 -1.6385145436707127 1104 1 -0.5841460130811456 1104 2 -0.16796342000110442 1104 3 -1.300646295623494 1104 4 -0.33105543548296623 1104 5 -0.431161763872403 1104 6 -0.3234207109905398 1104 7 -0.9708788620225448 1104 8 -0.712306276534818 1104 9 -0.8999100205488588 1104 10-11 -0.7088719062731101 1104 12-13 -1.2112467360793246 1104 14-15 -1.0965423257080005 1104 16-17 -1.0946401837081439 1104 18-19 -1.1062543262949305 1104 20-21 -0.8178462750919309 1104 22-23 -1.1610741539169709 1104 24-25 -0.6295905414447702 1104 26-27 -0.7279231995431843 1104 28-29 -0.8229693476295097 1104 30-31 -0.912479023858694 1104 32-33 -0.7555133196450612 1104 34-35 -0.7978268373295165 1104 36-37 -0.8171812078672858 1104 38-39 -1.0133616452054213 1104 40-41 -0.9845123318132281 1104 42-43 -0.7947141981981929 1104 44-45 -0.596471751664204 1104 46-47 -0.7740406310290666 1104 48-49 -0.6149046626003454 1104 50-51 -1.1089936458079919 1104 52-53 -1.2574453055327268 1104 54-55 -0.8159895645129396 1104 56-57 -0.6577868259438588 1104 58-59 -1.2742980816862897 1104 60-61 -0.47242746511167155 1104 62-63 -1.0515706282041037 1104 64-65 -0.9989412054834546 1104 66-67 -0.5805279575333273 1104 68-69 -0.5656515339731456 1104 70-71 -0.5141998693673528 1104 72-73 -0.24125904915418062 1104 74-75 -1.1486193240116833 1104 76 -0.8377569679131369 1105 1 -0.21801767544117823 1105 2 -0.7714733252551973 1105 3 -0.5381139958818899 1105 4 -0.006356899737919974 1105 5 -0.41430129875612565 1105 6 0.0752011667011061 1105 7 -0.22479789733692712 1105 8 -0.6480728570688399 1105 9 -0.11018133837831101 1105 10-11 -0.3846471000715539 1105 12-13 -1.201233816182686 1105 14-15 -0.25164568488113304 1105 16-17 -0.6883740338373485 1105 18-19 -0.2398675907221488 1105 20-21 -0.5316490313365492 1105 22-23 -0.20897467071283415 1105 24-25 -0.4283954510054926 1105 26-27 -0.21086893597730239 1105 28-29 -0.31997623824105403 1105 30-31 -0.4986116681308772 1105 32-33 -0.2660322688267982 1105 34-35 0.05382044949219278 1105 36-37 -0.23525829658390762 1105 38-39 -0.08591763142419495 1105 40-41 -0.024488645336056436 1105 42-43 -0.2355841379053416 1105 44-45 -0.6288468593300109 1105 46-47 -0.32373916677411074 1105 48-49 -0.30174790118776684 1105 50-51 -0.29383430041608705 1105 52-53 -0.4846416879616626 1105 54-55 -0.8364999004302547 1105 56-57 -0.09706331173197214 1105 58-59 -0.5598385640290928 1105 60-61 -0.18888956674819468 1105 62-63 -0.4734698530103074 1105 64-65 -0.42563155862729474 1105 66-67 -0.37492933565564357 1105 68-69 -0.3949689327672843 1105 70-71 -0.24644577806675017 1105 72-73 -0.5489033213333414 1105 74-75 -0.25321665389971315 1105 76 -0.9770980531844558 1106 1 0.2487201099225942 1106 2 0.008684558252380015 1106 3 0.9200468746109607 1106 4 0.16151167290982826 1106 5 0.68237034621934 1106 6 0.5600315486085066 1106 7 0.2272183177260132 1106 8 0.2160406483717452 1106 9 0.6616343219587861 1106 10-11 -0.02270982676240152 1106 12-13 0.7460672292621666 1106 14-15 0.5213220983490814 1106 16-17 0.48244952067513935 1106 18-19 0.6343589934468312 1106 20-21 0.324058244106169 1106 22-23 0.5435002599464411 1106 24-25 0.22355675723005675 1106 26-27 0.44944683103784655 1106 28-29 0.2697506659620643 1106 30-31 0.11750738456808563 1106 32-33 0.29734515809873585 1106 34-35 0.5590156703157447 1106 36-37 -0.013175941132658409 1106 38-39 0.526542364308682 1106 40-41 0.3947987453189441 1106 42-43 0.6230567837929755 1106 44-45 0.520139699236239 1106 46-47 0.6565573981928097 1106 48-49 0.3820797070904476 1106 50-51 0.2716553518134788 1106 52-53 0.5622539809096807 1106 54-55 0.2611638503698259 1106 56-57 0.5297768845556305 1106 58-59 -0.10786502018441269 1106 60-61 0.17242897389613887 1106 62-63 0.3861205032943644 1106 64-65 0.23719114246081574 1106 66-67 0.27540136628589806 1106 68-69 -0.23030070148928417 1106 70-71 0.053991601920444054 1106 72-73 0.1866631390891058 1106 74-75 -0.13037688432429206 1106 76 -0.273919047423842 1107 1 -0.5727666430569158 1107 2 0.17077835916123618 1107 3 -0.5348103662361616 1107 4 -0.14124062066815313 1107 5 0.12140832958554171 1107 6 -0.8402085268437745 1107 7 -0.5558563628878943 1107 8 -0.7701550131218724 1107 9 -0.12148322927159683 1107 10-11 -0.45926131437278883 1107 12-13 0.3392465141802816 1107 14-15 -0.44324533713064085 1107 16-17 0.04879179967711167 1107 18-19 -0.007907147340269205 1107 20-21 -0.26847798156475733 1107 22-23 0.006695835698820929 1107 24-25 -0.06410507934715781 1107 26-27 -0.06982351106967144 1107 28-29 -0.17443179138167153 1107 30-31 -0.40330629973269794 1107 32-33 -0.2976775633279942 1107 34-35 0.025576589461763888 1107 36-37 -0.6525697506156405 1107 38-39 -0.6755068511590423 1107 40-41 -0.31016567206937395 1107 42-43 -0.22263043218919165 1107 44-45 -0.10544977174035353 1107 46-47 -0.6309115206102334 1107 48-49 -0.5721130392012412 1107 50-51 -0.3098416901139842 1107 52-53 -0.5247090646189179 1107 54-55 -0.2614075292066076 1107 56-57 -0.722688175891939 1107 58-59 -0.5302906396648197 1107 60-61 -0.2858793515775844 1107 62-63 -0.21940551916983964 1107 64-65 -0.3656554664734202 1107 66-67 -0.05759614721834083 1107 68-69 -0.46568182126978286 1107 70-71 -0.1864696859128756 1107 72-73 0.04244638525219102 1107 74-75 0.24868510658713916 1107 76 -0.06633952243027252 1108 1 -0.08745289138802192 1108 2 -0.3085718856095703 1108 3 -0.7022335972107925 1108 4 -0.2650501444776765 1108 5 9.810932704539255E-4 1108 6 -0.16045774802757506 1108 7 -0.5020957932394765 1108 8 -0.23677717600571668 1108 9 -0.7582433538821931 1108 10-11 -0.11482428722548832 1108 12-13 -0.30926260909519954 1108 14-15 0.09115608699040934 1108 16-17 -0.0728147868827449 1108 18-19 -0.4651167601573647 1108 20-21 -0.22882511107076198 1108 22-23 -0.4696058999487178 1108 24-25 -0.25082070017492697 1108 26-27 0.34112441950443184 1108 28-29 0.1263692767091129 1108 30-31 0.09651568513601205 1108 32-33 -0.4629868646185997 1108 34-35 -0.36627921828189614 1108 36-37 -0.2091785623646345 1108 38-39 -0.09001508435886052 1108 40-41 0.05325221845131267 1108 42-43 0.10184664836265256 1108 44-45 -0.46201407970653463 1108 46-47 0.06352550653706857 1108 48-49 0.25162972893404856 1108 50-51 0.07936614255179109 1108 52-53 -0.34593736902478867 1108 54-55 -0.22769591371928755 1108 56-57 -0.12048523864227434 1108 58-59 -0.025951520839718967 1108 60-61 -0.08069466087886923 1108 62-63 0.5924769908435081 1108 64-65 0.5823418632996109 1108 66-67 -0.2675649945703711 1108 68-69 0.030446349624739355 1108 70-71 -0.22894854661603148 1108 72-73 -0.09297862587375505 1108 74-75 -0.09590768380004278 1108 76 0.2539096987535281 1109 1 -0.7332106868920114 1109 2 -1.063079983264874 1109 3 -0.7120958162100699 1109 4 -0.31890559528744333 1109 5 -0.3607302917911852 1109 6 -0.849459440074952 1109 7 -0.510265964861425 1109 8 -0.309293492176856 1109 9 -0.39716044356070057 1109 10-11 0.014266841611835446 1109 12-13 0.14612777040685643 1109 14-15 0.03748192697107555 1109 16-17 -0.32511113689483295 1109 18-19 0.1840493022066667 1109 20-21 0.07360418552097059 1109 22-23 -0.16449349965864712 1109 24-25 -0.05404767624454365 1109 26-27 -0.05195027230122662 1109 28-29 -0.3776553788383836 1109 30-31 -0.2670569520406758 1109 32-33 -0.07515751968266215 1109 34-35 -0.1405721841992289 1109 36-37 -0.5194396211028547 1109 38-39 0.3958200618823753 1109 40-41 -0.4784374117154684 1109 42-43 0.0633888286865556 1109 44-45 0.029151015773280164 1109 46-47 0.032971965323632446 1109 48-49 0.09953884181777539 1109 50-51 0.19266080051650647 1109 52-53 -0.40260887011253743 1109 54-55 -0.2159120123414766 1109 56-57 -0.38339314292073823 1109 58-59 -0.6292872713835962 1109 60-61 -0.5574410954933242 1109 62-63 -0.12750125426163805 1109 64-65 -0.0879264470702239 1109 66-67 -0.47458699118627834 1109 68-69 -0.06697932115118022 1109 70-71 0.2816146634395622 1109 72-73 0.049467591191749705 1109 74-75 0.24335990140662744 1109 76 -0.29059114727015967 1110 1 0.14549757523078455 1110 2 0.24998279254309708 1110 3 0.2825474508868382 1110 4 0.004488022547928239 1110 5 -0.6189758342241625 1110 6 0.9175992033962075 1110 7 -0.31295963626411805 1110 8 0.4825336639081428 1110 9 -0.03831035555722906 1110 10-11 -0.24383840543558932 1110 12-13 0.10079960674590893 1110 14-15 0.05469999701673345 1110 16-17 0.23652955386862828 1110 18-19 -0.3366889066038823 1110 20-21 -0.2924647377757168 1110 22-23 -0.1036690729566061 1110 24-25 -0.25085659392941295 1110 26-27 -0.09308232852141884 1110 28-29 -0.019251685243503402 1110 30-31 5.118564688757488E-4 1110 32-33 0.27490497553453963 1110 34-35 0.04524267690834449 1110 36-37 0.725171760679153 1110 38-39 0.04597438680649191 1110 40-41 0.09531969870975132 1110 42-43 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1.4278713175006672 2116 22-23 1.771911957194149 2116 24-25 0.9103400141107869 2116 26-27 0.9710351337901457 2116 28-29 0.8772621338519713 2116 30-31 1.4279144946598308 2116 32-33 0.8998107544290121 2116 34-35 1.3167564960038192 2116 36-37 0.8881428662067847 2116 38-39 1.2049253918316225 2116 40-41 0.7666450755941767 2116 42-43 0.801697838838848 2116 44-45 1.1235513964839399 2116 46-47 1.3330195541561167 2116 48-49 1.2438916336959522 2116 50-51 1.24737209493275 2116 52-53 1.1210269166894946 2116 54-55 1.096857657709279 2116 56-57 0.7679076185005869 2116 58-59 1.1700306220174141 2116 60-61 0.9544243867401789 2116 62-63 1.253637705129222 2116 64-65 1.1613597204424693 2116 66-67 1.056393338762966 2116 68-69 1.2688392067675949 2116 70-71 1.2842955010030153 2116 72-73 1.3460094693643399 2116 74-75 1.4210566019142377 2116 76 1.6205763654201952 2117 1 0.6499855322572969 2117 2 0.5039075889388691 2117 3 1.5549092599320637 2117 4 0.8518628276077074 2117 5 0.4817692706103571 2117 6 0.6556342059954119 2117 7 1.3596447650528063 2117 8 1.194749917590343 2117 9 0.7084233127844755 2117 10-11 1.5931395879797705 2117 12-13 1.1876996240739288 2117 14-15 1.68516265513491 2117 16-17 0.3064134561385998 2117 18-19 1.2068044221086467 2117 20-21 0.8945739037075597 2117 22-23 0.9754679916769078 2117 24-25 1.020361565834925 2117 26-27 0.8916170303418696 2117 28-29 0.6886845476450745 2117 30-31 0.9129360463839618 2117 32-33 0.5370952371876356 2117 34-35 0.9324892546245067 2117 36-37 1.0797376937929997 2117 38-39 1.0930719435557563 2117 40-41 0.9034985238700344 2117 42-43 0.7888745629767726 2117 44-45 0.8607281206218715 2117 46-47 1.1283859334664612 2117 48-49 0.5632881854200917 2117 50-51 1.3214022673465422 2117 52-53 0.6932251925515587 2117 54-55 0.9178705887437673 2117 56-57 0.655623135741962 2117 58-59 1.3686297599484547 2117 60-61 0.8427864557056921 2117 62-63 0.7533144292671565 2117 64-65 0.9670709273390159 2117 66-67 0.5743890284181319 2117 68-69 0.8134512757331152 2117 70-71 1.0910842941064587 2117 72-73 0.13674222798502456 2117 74-75 0.5880824639832056 2117 76 1.3826453309374394 2118 1 -0.019295857582132925 2118 2 0.46452880504837424 2118 3 1.1442222370313004 2118 4 -0.14887420845441213 2118 5 -0.09432970280370157 2118 6 0.8507381807256067 2118 7 0.9499580053792087 2118 8 -0.16371283576579998 2118 9 0.5679652975173042 2118 10-11 -0.23399913273620854 2118 12-13 0.903151058661976 2118 14-15 0.5789241713233793 2118 16-17 0.4703513345276491 2118 18-19 0.7274677545645574 2118 20-21 0.35966998162280106 2118 22-23 0.656620926659798 2118 24-25 0.72033524311842 2118 26-27 0.9756391414237342 2118 28-29 0.712901446829072 2118 30-31 0.976268502293415 2118 32-33 0.6892668612992452 2118 34-35 0.6743423738664092 2118 36-37 0.3861867593365602 2118 38-39 0.4106028727476456 2118 40-41 0.8088683580369249 2118 42-43 0.225147266319766 2118 44-45 0.22677181633126509 2118 46-47 0.23938882896527502 2118 48-49 0.7510481222455709 2118 50-51 0.5172432781388494 2118 52-53 0.7920669930253723 2118 54-55 0.5441143370985912 2118 56-57 0.6797873894929509 2118 58-59 0.20850867545253493 2118 60-61 -0.08193057509188151 2118 62-63 0.14149552955670686 2118 64-65 0.09444655250354117 2118 66-67 0.2844706288393013 2118 68-69 0.8253754663095805 2118 70-71 0.42630408878927284 2118 72-73 0.6808854235628132 2118 74-75 0.6222493500058377 2118 76 0.35721758679423843 2119 1 0.21734536379191027 2119 2 0.07521582794913684 2119 3 0.03735200802366734 2119 4 0.7213547991791742 2119 5 0.928571060555079 2119 6 0.3850893257637722 2119 7 0.6064465549975324 2119 8 0.4469741871349626 2119 9 0.7740721677463114 2119 10-11 0.5713443787141728 2119 12-13 0.025288463242134185 2119 14-15 -0.043213233256771844 2119 16-17 0.0924887391078073 2119 18-19 0.09387996830501066 2119 20-21 0.06196005795867876 2119 22-23 -0.18307372982876302 2119 24-25 0.09056425075200991 2119 26-27 0.006173492568777306 2119 28-29 0.01824495827945327 2119 30-31 -0.3748765358745203 2119 32-33 -0.3260003142732728 2119 34-35 0.09418970211068967 2119 36-37 -0.14053079791534628 2119 38-39 -0.3031375852676206 2119 40-41 0.3355859152888385 2119 42-43 -0.0153107489474138 2119 44-45 0.6847870835068477 2119 46-47 0.3767934091179441 2119 48-49 0.19379621384862844 2119 50-51 0.3340371712686263 2119 52-53 0.532525008292545 2119 54-55 0.21587006228943295 2119 56-57 -0.21334238149941598 2119 58-59 0.17034073652123283 2119 60-61 -0.1620832468476081 2119 62-63 -0.33560370708451615 2119 64-65 -0.05822520322165303 2119 66-67 -0.0819415849011591 2119 68-69 -0.15935735811790153 2119 70-71 -0.24545163640156176 2119 72-73 0.26867168310479883 2119 74-75 -0.0113384362537019 2119 76 0.12057636542019523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 6.0 13 125.0 14 652.0 15 1257.0 16 1628.0 17 1869.0 18 1928.0 19 1698.0 20 1299.0 21 853.0 22 667.0 23 528.0 24 412.0 25 394.0 26 425.0 27 470.0 28 546.0 29 661.0 30 844.0 31 1036.0 32 1398.0 33 1789.0 34 2764.0 35 4427.0 36 8871.0 37 33825.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.401361317550766 20.672701178008612 12.274593949383997 31.651343555056627 2 18.603725860770464 19.76041947906157 35.0091654469754 26.626689213192563 3 17.783809131342988 21.92460176488142 31.13978372387137 29.15180537990422 4 14.13752433461697 21.732766828186946 31.62150256490415 32.50820627229193 5 15.729043809415543 32.75546019069814 32.61051823852898 18.90497776135734 6 27.068620067355376 32.44141929433163 18.149006010828018 22.340954627484972 7 25.775876769169553 29.173534928664807 20.4911044165293 24.559483885636332 8 22.534210563710513 34.17930172083043 19.22186065678598 24.06462705867307 9 22.532789564179446 18.663407841075415 20.492234237562702 38.31156835718244 10-11 24.82557230756114 24.44261293393773 25.21705767837097 25.514757080130163 12-13 23.675983686925385 21.80168530544385 24.905858781066602 29.616472226564166 14-15 23.485569749761982 22.789279979537607 23.11682037144928 30.608329899251135 16-17 24.357352962073524 24.49163741775965 23.58575021670243 27.5652594034644 18-19 23.17010785386441 23.413098773677405 24.885254287866086 28.5315390845921 20-21 23.03440239864721 24.004234578602592 24.864649794665567 28.096713228084635 22-23 24.899464283176787 23.248262828073265 24.10086254671536 27.75141034203459 24-25 23.730692168871585 24.945646767936566 23.22765833487275 28.096002728319096 26-27 22.811305472269193 25.085615221746977 24.90301678200446 27.20006252397937 28-29 23.75058616230657 24.2394100009947 26.161311866767083 25.848691969931647 30-31 23.749165162775494 23.425887769457034 26.071078396544127 26.75386867122334 32-33 22.765122987509415 24.727523339917298 25.593622554104556 26.913731118468732 34-35 22.809884472738123 24.468190925496994 25.91121594929874 26.810708652466143 36-37 23.39249428047689 23.284498316115556 26.061131399826635 27.26187600358092 38-39 22.148409191024967 23.304392309550536 28.367413638753497 26.179784860670996 40-41 22.019808733463115 23.121083370042488 27.914114788342122 26.944993108152275 42-43 20.888693106731274 23.355548292669063 27.997243260909727 27.758515339689936 44-45 20.419763261478124 22.7729384849303 29.244880849189318 27.562417404402257 46-47 20.575362710130303 23.68379918434627 28.780924502294912 26.959913603228514 48-49 20.382106773904766 23.823057138391142 28.60827305926989 27.1865630284342 50-51 20.403421766870817 24.87459679138306 27.27466499936055 27.44731644238557 52-53 19.301436630525913 24.625921873445783 27.892089295610532 28.180552200417775 54-55 19.518849558779646 24.188254017876172 27.580890398306167 28.71200602503801 56-57 19.91175592912054 24.892359285521437 26.982649595725633 28.213235189632385 58-59 20.29755730180609 24.230884003808278 26.577664729370635 28.893893965014993 60-61 19.84283745186364 23.509726741790175 27.291716993733395 29.355718812612793 62-63 20.075881374959145 23.7775851533969 26.920125616358547 29.22640785528541 64-65 20.65991218222898 23.85431912807469 26.05615790146789 29.429610788228437 66-67 20.298267801571626 25.040143236752733 24.464638426669318 30.196950535006323 68-69 20.662043681525585 25.52399357708212 24.16409702584798 29.649865715544315 70-71 21.834368294658464 25.315106646014808 24.260724993960753 28.589800065365978 72-73 21.188524007787077 25.136771204865504 23.841530132295055 29.833174655052364 74-75 20.56754721270942 26.076051894902875 24.166228525144586 29.190172367243118 76 20.411237264291703 25.16021769712816 23.547383229363533 30.8811618092166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.0 15 2.0 16 2.5 17 1.0 18 3.5 19 9.5 20 13.0 21 13.0 22 28.0 23 51.0 24 67.5 25 76.0 26 119.5 27 189.5 28 253.0 29 290.0 30 383.5 31 558.0 32 789.0 33 939.0 34 1075.5 35 1332.0 36 1639.0 37 1826.0 38 2007.0 39 2520.0 40 3093.5 41 3418.5 42 3502.0 43 3646.5 44 3822.5 45 3930.0 46 4006.0 47 4048.5 48 3903.5 49 3544.0 50 3372.0 51 3183.5 52 2680.0 53 2176.0 54 1987.0 55 1911.5 56 1726.0 57 1506.5 58 1397.0 59 1361.5 60 1353.5 61 1390.0 62 1399.0 63 1475.0 64 1585.0 65 1620.5 66 1570.5 67 1519.0 68 1444.0 69 1312.0 70 1140.0 71 1025.0 72 914.5 73 679.5 74 436.5 75 318.0 76 228.0 77 104.0 78 45.5 79 21.0 80 16.0 81 9.5 82 5.0 83 2.0 84 2.0 85 2.5 86 4.5 87 6.0 88 4.5 89 3.5 90 2.5 91 2.0 92 3.0 93 3.0 94 2.0 95 2.0 96 3.0 97 1.5 98 1.0 99 8.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0014209995310701547 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.61542068691118 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33469936742114 52.96633652110895 2 11.867683836664355 16.048768703906326 3 4.1863690813946155 8.491893197675244 4 2.1099972679318246 5.706734116777742 5 1.1937036336506734 4.03563866823924 6 0.7965029527352205 3.231352933653532 7 0.5211945442700124 2.4668551859377885 8 0.2605972721350062 1.4096315348215935 9 0.20385431771851292 1.240532590624245 >10 0.5253977260786415 4.402256547255339 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.004262998593210464 0.0 3 0.0 0.0 0.0 0.004262998593210464 0.0 4 0.0 0.0 0.0 0.009946996717491084 0.0 5 0.0 0.0 0.0 0.009946996717491084 0.0 6 0.0 0.0 0.0 0.009946996717491084 0.0 7 0.0 0.0 0.0 0.009946996717491084 0.0 8 0.0 0.0 0.0 0.009946996717491084 0.0 9 0.0 0.0 0.0 0.012788995779631393 0.0 10 0.0 0.0 0.0 0.012788995779631393 0.0 11 0.0 0.0 0.0 0.012788995779631393 0.0 12 0.0 0.0 0.0 0.015630994841771703 0.0 13 0.0 0.0 0.0 0.015630994841771703 0.0 14 0.0 0.0 0.0 0.017051994372841855 0.0 15 0.0 0.0 0.0 0.017051994372841855 0.0 16 0.0 0.0 0.0 0.017051994372841855 0.0 17 0.0 0.0 0.0 0.01847299390391201 0.0 18 0.0 0.0 0.0 0.02415699202819263 0.0 19 0.0 0.0 0.0 0.02984099015247325 0.0 20 0.0 0.0 0.0 0.031261989683543406 0.0 21 0.0 0.0 0.0 0.039787986869964334 0.0 22 0.0 0.0 0.0 0.05115598311852557 0.0 23 0.0 0.0 0.0 0.052576982649595726 0.0 24 0.0 0.0 0.0 0.05826098077387634 0.0 25 0.0 0.0 0.0 0.06252397936708681 0.0 26 0.0 0.0 0.0 0.0753129751467182 0.0 27 0.0 0.0 0.0 0.16483594560413795 0.0 28 0.0 0.0 0.0 0.24725391840620692 0.0 29 0.0 0.0 0.0 0.3183038949597147 0.0 30 0.0 0.0 0.0 0.39077487104429254 0.0 31 0.0 0.0 0.0 0.4902448382192034 0.0 32 0.0 0.0 0.0 0.5911358049251844 0.0 33 0.0 0.0 0.0 0.7517087519361119 0.0 34 0.0 0.0 0.0 1.0188566637773009 0.0 35 0.0 0.0 0.0 1.3783695451380502 0.0 36 0.0 0.0 0.0 1.8657723842951133 0.0 37 0.0 0.0 0.0 2.4100152046949823 0.0 38 0.0 0.0 0.0 3.012519005868728 0.0 39 0.0 0.0 0.0 3.5851818168900005 0.0 40 0.0 0.0 0.0 4.209000611029798 0.0 41 0.0 0.0 0.0 4.984866354994103 0.0 42 0.0 0.0 0.0 5.69110312193597 0.0 43 0.0 0.0 0.0 6.447074872465292 0.0 44 0.0 0.0 0.0 7.208730621118895 0.0 45 0.0 0.0 0.0 8.018700353828883 0.0 46 0.0 0.0 0.0 8.844301081380642 0.0 47 0.0 0.0 0.0 9.584641837068194 0.0 48 0.0 0.0 0.0 10.252511616671166 0.0 49 0.0 0.0 0.0 10.82659542722351 0.0 50 0.0 0.0 0.0 11.39073224105836 0.0 51 0.0 0.0 0.0 11.997499040825316 0.0 52 0.0 0.0 0.0 12.685262813863272 0.0 53 0.0 0.0 0.0 13.30908160800307 0.0 54 0.0 0.0 0.0 13.947110397453569 0.0 55 0.0 0.0 0.0 14.595086183621559 0.0 56 0.0 0.0 0.0 15.189063987608884 0.0 57 0.0 0.0 0.0 15.818566779872963 0.0 58 0.0 0.0 0.0 16.385545592769954 0.0 59 0.0 0.0 0.0 16.98520739488156 0.0 60 0.0 0.0 0.0 17.62749918292527 0.0 61 0.0 0.0 0.0 18.228581984567946 0.0 62 0.0 0.0 0.0 18.754351811063902 0.0 63 0.0 0.0 0.0 19.271595640373437 0.0 64 0.0 0.0 0.0 19.83146945561508 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGAA 15 0.0022162087 70.0 2 GAAGCGG 15 0.0022162087 70.0 11 AATTTCC 20 0.006925075 52.5 16 AGGGTAA 20 0.006925075 52.5 12 CCTCGAT 20 0.006925075 52.5 36 CGGATGC 20 0.006925075 52.5 34 GGACCAC 20 0.006925075 52.5 16 GACTACA 20 0.006925075 52.5 40 TCTTACG 35 0.0012477514 40.0 11 TTACGCT 35 0.0012477514 40.0 13 CTTACGC 35 0.0012477514 40.0 12 AACAAGC 40 0.0024040798 35.0 32 CGTATCA 50 1.6942459E-4 35.0 53 TATACCC 50 1.6942459E-4 35.0 3 GCCGTAT 40 0.0024040798 35.0 51 ATCTTAC 65 2.0762494E-5 32.307693 10 ACATCTT 65 2.0762494E-5 32.307693 8 CGACGAA 45 0.0042812866 31.111109 19 GTATCAT 60 4.937447E-4 29.166666 54 TTCTACA 50 0.007165167 27.999998 2 >>END_MODULE