FastQCFastQC Report
Wed 25 May 2016
SRR1780198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70373
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT48046.826481747261023No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG18942.691373111846873RNA PCR Primer, Index 47 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG12421.764881417589132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC10661.514785500120785No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3760.5342958236823782No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2590.3680388785471701Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT1920.2728319099654697No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1550.22025492731587398No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1040.14778395123129612No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG960.13641595498273484No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT920.13073195685845423No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG900.12788995779631393No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGC150.002216208770.017
GCTTATA150.002216208770.03
CAGCTTA150.002216208770.01
CTTAATA200.00692507552.51
CTAAGGG200.00692507552.59
CCGCTGC200.00692507552.517
CTTTATA551.289227E-744.5454521
TGTATGG404.550752E-543.752
GCGGGCG350.001247751440.09
TCTTTAT350.001247751440.01
TAAGACC701.884473E-840.033
CTTGAAA11650.035.15021557
AAGACCT806.1099854E-835.034
CTAAGAC806.1099854E-835.032
ACCTCGT806.1099854E-835.037
GTGTATG601.2004213E-535.01
TAAGAAC501.6942459E-435.033
TGCTTGA11850.034.8523255
CGTATGC12200.034.71311641
TCGTATG12200.034.71311640
Produced by FastQC (version 0.11.5)