##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780198_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70373 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.54873317891805 34.0 34.0 34.0 34.0 34.0 2 33.60133858155827 34.0 34.0 34.0 34.0 34.0 3 33.637701959558356 34.0 34.0 34.0 34.0 34.0 4 33.66491410057834 34.0 34.0 34.0 34.0 34.0 5 33.65323348443295 34.0 34.0 34.0 34.0 34.0 6 37.319511744561126 38.0 38.0 38.0 36.0 38.0 7 37.30528753925511 38.0 38.0 38.0 36.0 38.0 8 37.158924587554885 38.0 38.0 38.0 36.0 38.0 9 37.188850837679226 38.0 38.0 38.0 36.0 38.0 10-11 37.131243516689636 38.0 38.0 38.0 36.0 38.0 12-13 37.110738493456296 38.0 38.0 38.0 36.0 38.0 14-15 37.016639904508835 38.0 38.0 38.0 35.0 38.0 16-17 37.03460133858155 38.0 38.0 38.0 35.5 38.0 18-19 36.956588464325804 38.0 37.5 38.0 35.0 38.0 20-21 37.006138717974224 38.0 38.0 38.0 35.0 38.0 22-23 37.007104997655354 38.0 38.0 38.0 35.0 38.0 24-25 36.9857900046893 38.0 37.5 38.0 35.0 38.0 26-27 36.84035070268427 38.0 37.0 38.0 34.5 38.0 28-29 36.79575263240163 38.0 37.0 38.0 34.5 38.0 30-31 36.79904224631606 38.0 37.0 38.0 34.5 38.0 32-33 36.61760902618902 38.0 37.0 38.0 34.0 38.0 34-35 36.70276952808605 38.0 37.0 38.0 34.0 38.0 36-37 36.72299745641084 38.0 37.0 38.0 34.0 38.0 38-39 35.879350034814486 38.0 37.0 38.0 32.0 38.0 40-41 36.2683557614426 38.0 37.0 38.0 34.0 38.0 42-43 36.61124294828983 38.0 37.0 38.0 34.0 38.0 44-45 36.730201924033366 38.0 37.0 38.0 34.0 38.0 46-47 36.654121609139864 38.0 37.0 38.0 34.0 38.0 48-49 36.67637446179643 38.0 37.0 38.0 34.0 38.0 50-51 36.4579099938897 38.0 37.0 38.0 34.0 38.0 52-53 36.45987097324257 38.0 37.0 38.0 34.0 38.0 54-55 36.404096741648075 38.0 37.0 38.0 34.0 38.0 56-57 36.457661318971766 38.0 37.0 38.0 34.0 38.0 58-59 36.30465519446379 38.0 37.0 38.0 34.0 38.0 60-61 36.31355775652594 38.0 37.0 38.0 34.0 38.0 62-63 36.28350361644381 38.0 37.0 38.0 34.0 38.0 64-65 36.14639137169085 38.0 37.0 38.0 33.0 38.0 66-67 35.6723530331235 38.0 37.0 38.0 31.0 38.0 68-69 35.55670498628736 38.0 37.0 38.0 31.0 38.0 70-71 34.72589629545422 38.0 35.5 38.0 25.5 38.0 72-73 33.566744347974364 38.0 34.0 38.0 20.0 38.0 74-75 33.25323632643201 38.0 34.0 38.0 15.0 38.0 76 32.46586048626604 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10916286359145744 1101 2 0.2164496587184317 1101 3 0.11876912979605692 1101 4 0.0780027046346703 1101 5 -0.1301630474379749 1101 6 0.17530784509097685 1101 7 0.24771606493150955 1101 8 -0.013039767030967653 1101 9 0.08926176567803878 1101 10-11 0.23303491806145615 1101 12-13 0.39709563240573686 1101 14-15 0.49536790915106366 1101 16-17 0.2902461863148389 1101 18-19 0.4211836124514008 1101 20-21 0.2883459867577045 1101 22-23 0.39853477277105753 1101 24-25 0.3075653523803794 1101 26-27 0.4370096737885234 1101 28-29 0.11386939269952734 1101 30-31 0.18612736685378195 1101 32-33 0.14889509719566263 1101 34-35 0.2883287240556811 1101 36-37 0.3461193536778211 1101 38-39 0.030328202020022843 1101 40-41 0.4998066421623619 1101 42-43 0.4714356243033535 1101 44-45 0.4931362719332455 1101 46-47 0.41147708734251864 1101 48-49 0.39602446333734065 1101 50-51 0.23576396881708206 1101 52-53 0.18940273371534744 1101 54-55 0.2843090881674186 1101 56-57 0.32568577275069543 1101 58-59 0.601670682585727 1101 60-61 0.4009119024139949 1101 62-63 -0.03467179994662217 1101 64-65 0.26760366513584444 1101 66-67 0.2835588508164264 1101 68-69 -0.3803558306430048 1101 70-71 -0.3358565109089682 1101 72-73 -0.3101135247916815 1101 74-75 -0.5915330480091043 1101 76 -0.17185427851410395 1102 1 0.10094601231884326 1102 2 0.11637034514139089 1102 3 -0.03344083487335325 1102 4 -0.27198647987755464 1102 5 -0.3228950396508239 1102 6 0.027207765777404802 1102 7 0.06406908245260468 1102 8 0.3277923044954605 1102 9 -0.03302534947085434 1102 10-11 -0.007862046391643673 1102 12-13 0.22314653730155243 1102 14-15 0.3379665103479752 1102 16-17 0.21212231292815176 1102 18-19 0.2685049766449197 1102 20-21 0.29123011683196864 1102 22-23 0.19783537122805228 1102 24-25 0.23351531272358983 1102 26-27 0.21850076903692184 1102 28-29 -0.03278862157691265 1102 30-31 0.22344079968960529 1102 32-33 0.303520520802266 1102 34-35 0.32499322762479466 1102 36-37 0.1178404582996393 1102 38-39 0.1815647727893719 1102 40-41 0.5015731718328524 1102 42-43 0.35206869085465087 1102 44-45 0.3917590998227851 1102 46-47 0.5558999008114043 1102 48-49 0.5066308516854576 1102 50-51 0.08359005577359824 1102 52-53 0.05024345763198568 1102 54-55 0.06713409357516298 1102 56-57 0.23195154533704 1102 58-59 0.4581491597650498 1102 60-61 0.346329740758506 1102 62-63 0.11555532529672519 1102 64-65 0.4954138893769624 1102 66-67 0.4372469321488879 1102 68-69 0.5765985279985628 1102 70-71 0.24129396857765784 1102 72-73 0.3243950915469185 1102 74-75 0.5685722227386094 1102 76 1.0664469688721567 1103 1 -0.0316187658981093 1103 2 0.0031072797044089384 1103 3 -0.050656469480294675 1103 4 0.05034553593272051 1103 5 0.19179534243086493 1103 6 -0.07202367910621632 1103 7 -0.05022604271209019 1103 8 0.044151549115575506 1103 9 -0.025098252413322086 1103 10-11 0.033453280793246165 1103 12-13 0.21100336646273377 1103 14-15 0.15592874135368362 1103 16-17 0.12527558477707146 1103 18-19 0.22498279834013601 1103 20-21 0.2340605955320143 1103 22-23 0.2622842074160161 1103 24-25 -0.09791859153640559 1103 26-27 -0.04245663105091069 1103 28-29 -0.026892705898390545 1103 30-31 -0.06746829121949105 1103 32-33 -0.054406576255260575 1103 34-35 -0.39447317553725725 1103 36-37 0.06064897828206739 1103 38-39 -0.19897980340737575 1103 40-41 0.2266610067032957 1103 42-43 0.40024611729295145 1103 44-45 0.16759353109200248 1103 46-47 -0.04002675707177872 1103 48-49 0.08997516437759856 1103 50-51 -0.2854481523950483 1103 52-53 -0.22674380631179503 1103 54-55 -0.08625852829572267 1103 56-57 -0.3672359821113602 1103 58-59 0.13123875133290142 1103 60-61 -0.2132332807610382 1103 62-63 0.2133155361004171 1103 64-65 0.306601855999709 1103 66-67 -0.5164750704861589 1103 68-69 -0.4480062150844404 1103 70-71 -0.8537104231658645 1103 72-73 -1.070323836867665 1103 74-75 -1.3042648435767177 1103 76 -0.7550066982336858 1104 1 0.19051928123994344 1104 2 0.3007503773475122 1104 3 0.173080904257084 1104 4 0.1144869500741379 1104 5 0.239101739737265 1104 6 0.13992918284664313 1104 7 0.19430257681653273 1104 8 0.24566670063072849 1104 9 0.06480073748566895 1104 10-11 0.17903408887405448 1104 12-13 0.28474074020010676 1104 14-15 0.134464094889033 1104 16-17 0.12506514706663552 1104 18-19 0.07275215860949658 1104 20-21 0.16794106691248345 1104 22-23 0.14570171583352476 1104 24-25 0.19959824348043043 1104 26-27 0.40771171911742954 1104 28-29 0.10740864090296043 1104 30-31 0.20029265154145293 1104 32-33 0.12004628569759745 1104 34-35 0.35186520830112755 1104 36-37 0.16262709276018228 1104 38-39 -0.07839899532657313 1104 40-41 0.26445561949790886 1104 42-43 0.20744308698991887 1104 44-45 0.47247568597415324 1104 46-47 0.03758267053765252 1104 48-49 0.019815231717672077 1104 50-51 -0.14386566081255125 1104 52-53 0.01109289402490532 1104 54-55 -0.31933933637652956 1104 56-57 -0.3016635915389685 1104 58-59 0.2867522193463685 1104 60-61 0.3025495475217923 1104 62-63 -0.011558201273324187 1104 64-65 0.31455640145425434 1104 66-67 -0.23596160247268472 1104 68-69 -0.16404156861978691 1104 70-71 -0.3639797834352194 1104 72-73 -0.47049218703601525 1104 74-75 -0.37345676276864026 1104 76 -0.7004612436882311 1105 1 0.03571738632176391 1105 2 0.19782186744225783 1105 3 0.21868813939919818 1105 4 -0.15166292072689913 1105 5 0.1686452367226181 1105 6 0.02554503125319485 1105 7 0.18243780506804086 1105 8 -0.12952709781888672 1105 9 0.028495827924949424 1105 10-11 0.24767103977844585 1105 12-13 0.06988285906315639 1105 14-15 0.2185080225376126 1105 16-17 0.0953601513732707 1105 18-19 0.14253036705049738 1105 20-21 -0.024554626455305595 1105 22-23 0.011227124963582469 1105 24-25 0.19780022453125667 1105 26-27 0.3042664133465536 1105 28-29 0.11153224124748817 1105 30-31 0.22344079968960529 1105 32-33 0.2094412038715916 1105 34-35 -0.04790869350765803 1105 36-37 -0.12640176167565187 1105 38-39 0.01349376953130843 1105 40-41 -0.27064343304299854 1105 42-43 -0.09827396727364857 1105 44-45 0.049887400013780336 1105 46-47 -0.16174980146923446 1105 48-49 -0.14231870454418072 1105 50-51 0.12723683703413258 1105 52-53 0.45739995690173174 1105 54-55 0.10417487550977 1105 56-57 0.5174363223283933 1105 58-59 0.3009533390707446 1105 60-61 -0.029459496405863206 1105 62-63 -0.41268869222939486 1105 64-65 -0.3252691799410954 1105 66-67 -0.34327211065528473 1105 68-69 -0.029771542779990057 1105 70-71 0.29792805463539906 1105 72-73 0.562604281525573 1105 74-75 -0.22487795139912947 1105 76 0.6890736400326958 1106 1 0.28944045011523656 1106 2 -0.051271369169647585 1106 3 -0.13247465129847313 1106 4 0.0752412823881059 1106 5 -0.1999060754173385 1106 6 0.15454012406308948 1106 7 -0.22724856147772954 1106 8 0.22884712876528113 1106 9 0.05842968753663769 1106 10-11 0.19961258020942552 1106 12-13 0.05946041400234492 1106 14-15 0.25309304494000884 1106 16-17 -0.11680114583176504 1106 18-19 0.14153826122587532 1106 20-21 0.15313899419756893 1106 22-23 0.01645434920084199 1106 24-25 0.13433745516918805 1106 26-27 -0.17264845757301828 1106 28-29 0.2529446585720052 1106 30-31 -0.2949078241636087 1106 32-33 0.18454254801496006 1106 34-35 -0.18069085693842624 1106 36-37 -0.11130681821501298 1106 38-39 -0.13580215536735096 1106 40-41 -0.14567010255441204 1106 42-43 -0.0955003380865378 1106 44-45 -0.0448960435535426 1106 46-47 0.128009106147573 1106 48-49 0.1653087757203906 1106 50-51 2.899627008687844E-4 1106 52-53 0.03672575165317227 1106 54-55 0.17097666770094122 1106 56-57 0.38772648661257847 1106 58-59 0.16011698319278622 1106 60-61 0.12027380847999325 1106 62-63 0.17186432675929808 1106 64-65 0.3655885115459938 1106 66-67 0.5009352714654725 1106 68-69 0.5504716831641048 1106 70-71 0.17675246257224586 1106 72-73 0.6201890905718699 1106 74-75 0.636227233153889 1106 76 0.8752268297062642 1107 1 0.2112444456566962 1107 2 0.0144921565098457 1107 3 0.13388048888844395 1107 4 0.028297957343085045 1107 5 0.032109700969520816 1107 6 0.09233485955138576 1107 7 0.16578925040600723 1107 8 0.3142025261758974 1107 9 0.2441880687421616 1107 10-11 0.19078123321465768 1107 12-13 0.29694219399034694 1107 14-15 0.38044021119225846 1107 16-17 0.47659249216874855 1107 18-19 0.4185681849161753 1107 20-21 0.2975057951921727 1107 22-23 0.5133462295060767 1107 24-25 0.2684153082087093 1107 26-27 0.12041507668060802 1107 28-29 0.4661616696326263 1107 30-31 -0.11768069563749606 1107 32-33 0.4982697194116881 1107 34-35 0.1069924149470225 1107 36-37 0.1309336002021979 1107 38-39 0.1810117004159011 1107 40-41 0.4205649999063539 1107 42-43 0.23100227702106935 1107 44-45 0.3523216534369382 1107 46-47 0.13004546043034537 1107 48-49 0.08655545324760539 1107 50-51 0.396200105888731 1107 52-53 0.14619587083348762 1107 54-55 0.21622660339501465 1107 56-57 -0.27964040012325597 1107 58-59 0.14005353468039772 1107 60-61 -0.08186114822068191 1107 62-63 0.3343010925999934 1107 64-65 0.6410750930430424 1107 66-67 0.7773864418333076 1107 68-69 0.4165010066657757 1107 70-71 -0.026968706938170328 1107 72-73 0.341337165681189 1107 74-75 0.4531268301700919 1107 76 -0.1794332063050561 1108 1 -0.15564473992407812 1108 2 0.020856197453326786 1108 3 0.12466820584438665 1108 4 -0.025195589608401292 1108 5 0.05391655455207456 1108 6 -0.5389067959893339 1108 7 -0.20239054487659303 1108 8 -0.10195234698831968 1108 9 -0.18202465933972434 1108 10-11 -0.1779103555703898 1108 12-13 -0.22081481535545322 1108 14-15 0.09835298377792157 1108 16-17 0.018782078283564374 1108 18-19 0.1132944866518244 1108 20-21 -0.12643723996582423 1108 22-23 -0.4636898185580094 1108 24-25 -0.17811339673121296 1108 26-27 -0.0944046829989631 1108 28-29 -0.11964162364731123 1108 30-31 0.11391699016579793 1108 32-33 -0.29509921694790364 1108 34-35 0.23420647814239715 1108 36-37 0.47049746313054897 1108 38-39 0.3789422745146922 1108 40-41 0.3083709634132603 1108 42-43 0.048514515561343785 1108 44-45 0.3099095484080152 1108 46-47 0.44882605678103715 1108 48-49 0.22623057563301074 1108 50-51 0.23100206405517554 1108 52-53 0.06459818503019932 1108 54-55 -0.01775203478922549 1108 56-57 0.27042635555098116 1108 58-59 0.15494005003419886 1108 60-61 0.6154463729186119 1108 62-63 0.03864021142508989 1108 64-65 0.6222944966923478 1108 66-67 0.2810357520246711 1108 68-69 0.04409335201513187 1108 70-71 1.0020255075700604 1108 72-73 0.5137670722232386 1108 74-75 0.5368606975488177 1108 76 1.0352530420260564 1109 1 0.09729217088084852 1109 2 0.09027848413450101 1109 3 -0.03348987972486839 1109 4 0.12351120275321392 1109 5 0.12329049702246664 1109 6 0.07769944041385912 1109 7 0.28329956873230344 1109 8 0.02400849420094886 1109 9 0.11908725750823379 1109 10-11 -0.18704741140795278 1109 12-13 0.004565895304452283 1109 14-15 0.06755772151058181 1109 16-17 0.029405718602646402 1109 18-19 0.060285059530720275 1109 20-21 0.12811920190026882 1109 22-23 0.019667686880715962 1109 24-25 -0.10164494132874324 1109 26-27 0.10784332280233855 1109 28-29 0.11931172418928782 1109 30-31 -0.1369652916809585 1109 32-33 0.14886984275719328 1109 34-35 -0.059690065246535084 1109 36-37 -0.0235803710488085 1109 38-39 -0.05869369357812815 1109 40-41 0.358975266048013 1109 42-43 0.2005691445195268 1109 44-45 0.00408407100893271 1109 46-47 -0.1465449850367051 1109 48-49 -0.18230217756979528 1109 50-51 0.0259500940285875 1109 52-53 0.09568351256222485 1109 54-55 -0.3190573284803122 1109 56-57 -0.06042933697983699 1109 58-59 -0.26648852139437196 1109 60-61 0.3215498295296868 1109 62-63 0.020837455805072125 1109 64-65 -0.13817202494168157 1109 66-67 0.20127989028910775 1109 68-69 0.3384612514591723 1109 70-71 -0.2376167452701523 1109 72-73 -0.5735425724468044 1109 74-75 -0.008256537162324662 1109 76 -0.1712734264293445 1110 1 0.14126839719006057 1110 2 -0.25303707844999934 1110 3 0.0660178110130829 1110 4 -0.110527008608166 1110 5 0.12091822959395415 1110 6 -0.12677470697282445 1110 7 -0.09899260278518085 1110 8 0.2741424125235241 1110 9 0.3628186045990134 1110 10-11 0.10550672985674936 1110 12-13 -0.030865066611731606 1110 14-15 -0.22141251035943554 1110 16-17 0.06691560305026201 1110 18-19 0.06038709253839869 1110 20-21 0.2077814726448537 1110 22-23 -0.06035169939074336 1110 24-25 -0.034430697520868137 1110 26-27 0.14091477141596442 1110 28-29 0.20324662713039032 1110 30-31 -0.2916345771948201 1110 32-33 0.025767018993704482 1110 34-35 -0.015841380196924604 1110 36-37 -0.042902871877828375 1110 38-39 0.0013399773144087135 1110 40-41 0.1574855841359195 1110 42-43 -0.02821436694642898 1110 44-45 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-0.23957331135265036 2116 2 -0.05711999302285875 2116 3 0.09877534870152971 2116 4 -0.21954082770363925 2116 5 -0.012750112114588319 2116 6 0.007521775439236933 2116 7 -0.342271497257542 2116 8 0.14983336729738994 2116 9 0.0641062930412275 2116 10-11 0.08458964442961303 2116 12-13 0.10731018464454678 2116 14-15 -0.16310534955540845 2116 16-17 -0.3946107788592883 2116 18-19 -0.12643765620532577 2116 20-21 -0.15173485901343753 2116 22-23 -0.12038624712943857 2116 24-25 -0.22320268244549624 2116 26-27 -0.39217254014182146 2116 28-29 -0.24166543317111433 2116 30-31 -0.3859342003103947 2116 32-33 -0.04048612170980448 2116 34-35 -0.10612090280998387 2116 36-37 -0.14304420353612102 2116 38-39 -0.335343439771016 2116 40-41 -0.6704980842057964 2116 42-43 -0.6415152463434168 2116 44-45 -0.26166783254436865 2116 46-47 -0.12781084798086084 2116 48-49 -0.15888847198603884 2116 50-51 -0.030902697849590766 2116 52-53 -0.17930062449361373 2116 54-55 -0.19676989193209238 2116 56-57 -0.09564507302044944 2116 58-59 -0.3039885213943734 2116 60-61 -0.1842560080337634 2116 62-63 -0.16850264571777274 2116 64-65 -0.2916935985457485 2116 66-67 -0.1548273432134195 2116 68-69 -0.04147212417534263 2116 70-71 0.4602100313795887 2116 72-73 0.8762670722232428 2116 74-75 0.8633487927869155 2116 76 0.7995387563117689 2117 1 0.2108577231301041 2117 2 0.24417311042541456 2117 3 0.11924948663256174 2117 4 -0.00811841391053747 2117 5 0.19414643960954692 2117 6 0.12562522371509743 2117 7 0.3107457441217676 2117 8 -0.446287322357783 2117 9 -0.39860922420015044 2117 10-11 -0.0020482866048681103 2117 12-13 0.024012770851442156 2117 14-15 0.17881275389286344 2117 16-17 -0.14008491679032886 2117 18-19 0.3635407920705376 2117 20-21 0.10279100305552191 2117 22-23 0.3129111666636675 2117 24-25 0.1482412830717479 2117 26-27 0.3139697012374896 2117 28-29 -0.1412343986883542 2117 30-31 0.07516493762063448 2117 32-33 0.06910439553156777 2117 34-35 0.2342885799486325 2117 36-37 0.2141756240500854 2117 38-39 0.6681048360910538 2117 40-41 -0.4030411876540754 2117 42-43 0.22626492607037108 2117 44-45 -0.31242214288919 2117 46-47 -0.4059358479808637 2117 48-49 -0.37656088577914204 2117 50-51 -0.29641993922889753 2117 52-53 0.19990196171328733 2117 54-55 0.31820855634377665 2117 56-57 -0.35297265922734766 2117 58-59 0.36109768550218035 2117 60-61 0.26725261265589495 2117 62-63 0.34529045773050626 2117 64-65 -0.25052980544229797 2117 66-67 0.1806252429934716 2117 68-69 -0.466579882796033 2117 70-71 -0.039682209999718054 2117 72-73 0.1758360377404884 2117 74-75 0.4579608617524329 2117 76 -1.2642543471365073 2118 1 -0.11485957089463739 2118 2 -0.3940474739388904 2118 3 -0.14392503297786163 2118 4 -0.06023739258913707 2118 5 -0.43717759303061854 2118 6 -0.51938662150733 2118 7 0.027671250834060857 2118 8 -0.7441551823209309 2118 9 -0.5571246229893063 2118 10-11 -0.1847805845780215 2118 12-13 -0.2956239756607886 2118 14-15 -0.37009485337220127 2118 16-17 -0.8204218475615761 2118 18-19 -1.0555168546786007 2118 20-21 -0.548538294127944 2118 22-23 -0.30103987308363855 2118 24-25 -0.03978188091877399 2118 26-27 -0.18360860884411068 2118 28-29 -0.45855474614820935 2118 30-31 -0.7765592003103947 2118 32-33 -0.6532962362136203 2118 34-35 -0.3532344524283033 2118 36-37 -0.27632187529184193 2118 38-39 -0.812442676412239 2118 40-41 -1.2579503742821245 2118 42-43 -0.7046116203892154 2118 44-45 -0.9038672600252866 2118 46-47 -0.8425770693549097 2118 48-49 -0.8809304567188647 2118 50-51 -1.011055369605316 2118 52-53 -1.735001960371477 2118 54-55 -1.0466506171229284 2118 56-57 -0.8279924012647228 2118 58-59 -1.1748854679592569 2118 60-61 -0.7010021912398656 2118 62-63 -0.661632416710134 2118 64-65 -0.8366840565610119 2118 66-67 -1.003896999701972 2118 68-69 -1.2076696432593153 2118 70-71 -1.441340541139489 2118 72-73 -0.8037901796851514 2118 74-75 -0.027824871335219825 2118 76 -1.8703085719325045 2119 1 0.10651447490688781 2119 2 -0.020001672412178095 2119 3 -0.07522274290152353 2119 4 0.17640382878490612 2119 5 -0.38374247852680554 2119 6 -0.7636614306676321 2119 7 -0.31584019954762255 2119 8 -0.3395750296491755 2119 9 -0.4960559206992343 2119 10-11 -0.69623096625741 2119 12-13 -0.9750132886378964 2119 14-15 -1.648720807570676 2119 16-17 -0.6944676490882955 2119 18-19 -0.6891046409381474 2119 20-21 -0.9378512712271814 2119 22-23 -0.5834826211752357 2119 24-25 -0.7115376061096157 2119 26-27 -0.13017349434029768 2119 28-29 -0.07687535683523805 2119 30-31 -0.2727424064172652 2119 32-33 -1.0616931827785123 2119 34-35 -1.1891123150237277 2119 36-37 -0.6580012646048203 2119 38-39 -0.7170228290839944 2119 40-41 -0.6472633513813619 2119 42-43 -0.9679703990151722 2119 44-45 -0.854248939414596 2119 46-47 -0.8578442449274277 2119 48-49 -0.8274953422150446 2119 50-51 -0.9270859039564598 2119 52-53 -0.6166813496844554 2119 54-55 -0.4245132125427773 2119 56-57 -0.22493896615021924 2119 58-59 -1.0680152389516238 2119 60-61 -0.5864983744459806 2119 62-63 0.22768056038910345 2119 64-65 -0.90538634663735 2119 66-67 -0.8359580684042669 2119 68-69 -1.016829948602826 2119 70-71 -0.8993558083150646 2119 72-73 -0.7923397980057629 2119 74-75 -0.024008077442090325 2119 76 -1.6642017017034973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 2.0 22 11.0 23 14.0 24 36.0 25 58.0 26 111.0 27 217.0 28 339.0 29 605.0 30 947.0 31 1431.0 32 2188.0 33 3474.0 34 5506.0 35 8170.0 36 11228.0 37 36034.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.27735068847427 17.275091299219874 13.13856166427465 35.30899634803121 2 18.684722834041466 20.11424836229804 36.76978386597132 24.43124493768917 3 17.70991715572734 18.983132735566198 33.92778480383101 29.37916530487545 4 14.13894533414804 25.595043553635627 32.97713611754508 27.288874994671254 5 14.425987239424213 30.386653972404186 40.555326616742214 14.632032171429382 6 26.986202094553306 38.725079220723856 16.88857942676879 17.400139257954045 7 25.866454464070028 25.487047589274297 22.942037429127648 25.704460517528027 8 22.092279709547697 37.33818367839938 16.475068563227374 24.094468048825544 9 21.307887968396972 16.42107058104671 17.651656174953462 44.61938527560286 10-11 25.30160715046964 27.742884344848168 24.124309038978016 22.831199465704177 12-13 24.512952410725706 19.365381609424066 26.285649325735722 29.836016654114506 14-15 21.54448439032015 18.935529251275344 20.60662469981385 38.91336165859065 16-17 26.848365140039505 26.133602375911213 19.449220581757206 27.568811902292072 18-19 29.52410725704461 24.39785144870902 20.1803248404928 25.89771645375357 20-21 23.990024583291888 22.410583604507412 20.11993236042232 33.47945945177838 22-23 21.84076279254828 25.74495900416353 21.497591405794836 30.91668679749336 24-25 28.292100663606778 25.914768448126413 21.58640387648672 24.206727011780085 26-27 27.298821991388746 27.01462208517471 22.503659073792505 23.182896849644038 28-29 22.596024043312067 25.687408523155185 25.169454194080114 26.54711323945263 30-31 26.86825913347449 21.625481363591152 28.618930555752915 22.887328947181448 32-33 23.765506657382804 20.91569209782161 27.330083981072285 27.988717263723302 34-35 21.322808463473205 29.720915692097822 24.984724255040998 23.97155158938798 36-37 27.955323774743157 27.921930285763008 22.340244127719437 21.782501811774402 38-39 22.02122973299419 24.847597800292725 27.64199337814219 25.4891790885709 40-41 21.85355178832791 19.95154391599051 28.55924857544797 29.63565572023361 42-43 26.9130206187032 19.647450016341494 27.71020135563355 25.729328009321755 44-45 20.511417731232147 25.298054651641966 30.844926321174317 23.345601295951575 46-47 24.573344890796186 21.06276554928737 28.680744035354465 25.68314552456198 48-49 24.27422448950592 20.13698435479516 24.77796882327029 30.81082233242863 50-51 22.479502081764313 20.551916217867646 28.069003737228766 28.899577963139272 52-53 18.323788953149645 20.459551248348088 36.91756781720262 24.299091981299647 54-55 19.49540306651699 22.304008639677146 30.717036363377996 27.483551930427865 56-57 23.31291830673696 23.611328208261693 25.403208616941157 27.672544868060196 58-59 19.778892472965484 23.873502621744137 31.124863228795135 25.222741676495247 60-61 22.90438094155429 28.061188239807883 25.81671948048257 23.217711338155258 62-63 19.115285691955723 35.8923166555355 21.992809742372785 22.99958791013599 64-65 18.158242507779974 37.29200119363961 20.80556463416367 23.74419166441675 66-67 18.1831099995737 37.066772767964984 20.792065138618504 23.95805209384281 68-69 17.85841160672417 37.026274281329485 21.278046978244497 23.837267133701843 70-71 18.207977491367426 35.12355590922655 21.819447799582225 24.849018799823796 72-73 18.44883691188382 31.891492475807482 22.780753982351186 26.878916629957512 74-75 18.55825387577622 31.204439202535063 23.590013215295638 26.64729370639308 76 18.64635584670257 31.40835263524363 23.41949327156722 26.525798246486577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 1.0 15 0.5 16 2.5 17 4.0 18 4.5 19 6.0 20 13.0 21 19.0 22 16.0 23 23.0 24 42.0 25 51.0 26 96.5 27 153.0 28 201.5 29 239.0 30 304.5 31 427.0 32 601.5 33 719.0 34 829.0 35 1043.0 36 1252.0 37 1357.0 38 1640.0 39 3268.0 40 5441.5 41 6062.0 42 5854.0 43 5270.5 44 4382.5 45 3908.0 46 3738.0 47 3631.5 48 3383.0 49 2935.0 50 2629.0 51 2466.0 52 2033.0 53 1684.5 54 1606.0 55 1580.5 56 1405.0 57 1230.5 58 1206.0 59 1176.0 60 1204.0 61 1249.5 62 1237.0 63 1244.5 64 1335.0 65 1426.0 66 1389.0 67 1344.0 68 1359.0 69 1277.5 70 1050.5 71 920.0 72 853.0 73 671.0 74 444.0 75 332.0 76 235.5 77 87.0 78 23.0 79 11.0 80 6.0 81 0.5 82 0.5 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.29551106248135 #Duplication Level Percentage of deduplicated Percentage of total 1 67.38753052637773 32.545152260099755 2 17.730308647424014 17.125886348457506 3 6.2847559360932115 9.105764995097552 4 3.0835319386824374 5.956830034246089 5 1.7389001677111837 4.199053614312307 6 1.135728366728455 3.2910349139584785 7 0.7649983817341925 2.586219146547682 8 0.47959513931797454 1.8529833885154816 9 0.3619030805896372 1.5730464808946614 >10 0.985670991849825 6.680118795560798 >50 0.02059611027745903 0.7431827547496909 >100 0.014711507341042164 1.5432054907421882 >500 0.0 0.0 >1k 0.01176920587283373 12.797521776817813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 4804 6.826481747261023 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 1894 2.691373111846873 RNA PCR Primer, Index 47 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 1242 1.764881417589132 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 1066 1.514785500120785 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 376 0.5342958236823782 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 259 0.3680388785471701 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 192 0.2728319099654697 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 155 0.22025492731587398 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 104 0.14778395123129612 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 96 0.13641595498273484 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT 92 0.13073195685845423 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 90 0.12788995779631393 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.004262998593210464 0.0 3 0.0 0.0 0.0 0.004262998593210464 0.0 4 0.0 0.0 0.0 0.009946996717491084 0.0 5 0.0 0.0 0.0 0.009946996717491084 0.0 6 0.0 0.0 0.0 0.009946996717491084 0.0 7 0.0 0.0 0.0 0.009946996717491084 0.0 8 0.0 0.0 0.0 0.009946996717491084 0.0 9 0.0 0.0 0.0 0.012788995779631393 0.0 10 0.0 0.0 0.0 0.012788995779631393 0.0 11 0.0 0.0 0.0 0.012788995779631393 0.0 12 0.0 0.0 0.0 0.015630994841771703 0.0 13 0.0 0.0 0.0 0.015630994841771703 0.0 14 0.0 0.0 0.0 0.015630994841771703 0.0 15 0.0 0.0 0.0 0.015630994841771703 0.0 16 0.0 0.0 0.0 0.015630994841771703 0.0 17 0.0 0.0 0.0 0.017051994372841855 0.0 18 0.0 0.0 0.0 0.022735992497122475 0.0 19 0.0 0.0 0.0 0.028419990621403095 0.0 20 0.0 0.0 0.0 0.02984099015247325 0.0 21 0.0 0.0 0.0 0.039787986869964334 0.0 22 0.0 0.0 0.0 0.04831398405638526 0.0 23 0.0 0.0 0.0 0.05115598311852557 0.0 24 0.0 0.0 0.0 0.05826098077387634 0.0 25 0.0 0.0 0.0 0.0596819803049465 0.0 26 0.0 0.0 0.0 0.07389197561564805 0.0 27 0.0 0.0 0.0 0.16199394654199764 0.0 28 0.0 0.0 0.0 0.24583291887513678 0.0 29 0.0 0.0 0.0 0.31261989683543406 0.0 30 0.0 0.0 0.0 0.3865118724510821 0.0 31 0.0 0.0 0.0 0.4845608400949228 0.0 32 0.0 0.0 0.0 0.5911358049251844 0.0 33 0.0 0.0 0.0 0.7502877524050418 0.0 34 0.0 0.0 0.0 1.01033066659088 0.0 35 0.0 0.0 0.0 1.358475551703068 0.0 36 0.0 0.0 0.0 1.8330893950804996 0.0 37 0.0 0.0 0.0 2.371648217356088 0.0 38 0.0 0.0 0.0 2.967047020874483 0.0 39 0.0 0.0 0.0 3.532604834240405 0.0 40 0.0 0.0 0.0 4.157844627911273 0.0 41 0.0 0.0 0.0 4.922342375627016 0.0 42 0.0 0.0 0.0 5.631421141631023 0.0 43 0.0 0.0 0.0 6.376024895911784 0.0 44 0.0 0.0 0.0 7.103576655819704 0.0 45 0.0 0.0 0.0 7.907862390405411 0.0 46 0.0 0.0 0.0 8.739147116081451 0.0 47 0.0 0.0 0.0 9.463856876927231 0.0 48 0.0 0.0 0.0 10.124621658874853 0.0 49 0.0 0.0 0.0 10.705810467082546 0.0 50 0.0 0.0 0.0 11.262842283262046 0.0 51 0.0 0.0 0.0 11.856820087249371 0.0 52 0.0 0.0 0.0 12.534636863569835 0.0 53 0.0 0.0 0.0 13.151350660054282 0.0 54 0.0 0.0 0.0 13.76806445653873 0.0 55 0.0 0.0 0.0 14.391883250678527 0.0 56 0.0 0.0 0.0 14.97591405794836 0.0 57 0.0 0.0 0.0 15.596890853026018 0.0 58 0.0 0.0 0.0 16.1596066673298 0.0 59 0.0 0.0 0.0 16.753584471317126 0.0 60 0.0 0.0 0.0 17.395876259360833 0.0 61 0.0 0.0 0.0 17.99127506287923 0.0 62 0.0 0.0 0.0 18.497150895940205 0.0 63 0.0 0.0 0.0 18.98455373509727 0.0 64 0.0 0.0 0.0 19.538743552214626 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCGGC 15 0.0022162087 70.0 17 GCTTATA 15 0.0022162087 70.0 3 CAGCTTA 15 0.0022162087 70.0 1 CTTAATA 20 0.006925075 52.5 1 CTAAGGG 20 0.006925075 52.5 9 CCGCTGC 20 0.006925075 52.5 17 CTTTATA 55 1.289227E-7 44.545452 1 TGTATGG 40 4.550752E-5 43.75 2 GCGGGCG 35 0.0012477514 40.0 9 TCTTTAT 35 0.0012477514 40.0 1 TAAGACC 70 1.884473E-8 40.0 33 CTTGAAA 1165 0.0 35.150215 57 AAGACCT 80 6.1099854E-8 35.0 34 CTAAGAC 80 6.1099854E-8 35.0 32 ACCTCGT 80 6.1099854E-8 35.0 37 GTGTATG 60 1.2004213E-5 35.0 1 TAAGAAC 50 1.6942459E-4 35.0 33 TGCTTGA 1185 0.0 34.85232 55 CGTATGC 1220 0.0 34.713116 41 TCGTATG 1220 0.0 34.713116 40 >>END_MODULE