FastQCFastQC Report
Wed 25 May 2016
SRR1780197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384746
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA250.002349201135.2048924
GTTTACG300.005747257329.32596227
GGTTTAC300.005747257329.32596226
GTTATAC454.0838517E-529.3107151
TCGTAAC406.9803593E-427.52889420
AGAATCC406.9963187E-427.51814340
ATTTGCG407.0336746E-427.49309230
CGTAACA450.001387961824.47012721
AGACAAG450.001392180324.45738813
TATGCAA551.5935769E-423.9939710
AGTCGTA500.002561595522.02311318
GAGACAA500.002573242422.00591912
TGCAATG500.002573242422.00591912
ATATAGA500.00259079421.9801794
GAAAATT755.470774E-520.52817526
GTTAGGA550.004461081720.01319514
GGTAAGT550.00448466419.99497629
AGTATCC550.00448466419.99497629
ATGCTCA550.00448804119.99237641
AAATTTG808.985592E-519.24516328
Produced by FastQC (version 0.11.5)