FastQCFastQC Report
Wed 25 May 2016
SRR1780197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384746
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT160784.1788608588523335No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG42581.1067041632661547No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC40011.0399068476345432No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG37530.9754487376087081RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC10210.26536988038862No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT5180.13463427819912357RNA PCR Primer, Index 35 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGTT207.852571E-443.9970114
TCTTCTG4200.039.2881544
TTAATAC357.2809744E-637.7117273
CGTCTTC4400.037.00229642
GTCTTCT4450.036.5865443
TCTTTAT1250.035.257181
GGTACTA250.0023500935.20218344
CTTTATA1250.035.197612
CCGTCTT4800.033.9187741
CTTAATA401.8177096E-532.9977572
ATGCCGT22750.032.10748344
ACAGTAA300.005742115429.33133919
GAAGCAA300.005742115429.33133917
TGCTACG300.005742115429.3313392
TAATACA852.6375346E-1028.4686554
TTTATAC1750.027.6552623
TATCCGC407.0208136E-427.50170538
TATGCCG27550.026.51344143
TAATACG508.3307736E-526.3982074
GCCGTCT6200.025.90483340
Produced by FastQC (version 0.11.5)