FastQCFastQC Report
Wed 25 May 2016
SRR1780196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43083
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAA150.002210346570.043
TAAGAGC150.002210346570.032
CTCGTAC200.006906897352.545
TAACGTC200.006906897352.516
AGTGAGC200.006906897352.58
GTCCGCG200.006906897352.523
CACGTAC200.006906897352.535
CAAAGAG200.006906897352.536
CAATCTC500.00713473828.0000025
TGACGCT4955.6216225E-47.77777770
ATCTGAC5652.7874042E-47.433628667
CTGACGC5300.00116589547.26415169
TCTGACG5350.00128821717.19626268
CATCTGA5800.00302308826.63793166
ACATCTG6450.0091464775.96899265
Produced by FastQC (version 0.11.5)