##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780196_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43083 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.051041013856974 34.0 31.0 34.0 12.0 34.0 2 30.060975326694983 34.0 31.0 34.0 12.0 34.0 3 29.958452289766264 34.0 31.0 34.0 11.0 34.0 4 29.820323561497574 34.0 31.0 34.0 11.0 34.0 5 29.748346215444606 34.0 31.0 34.0 11.0 34.0 6 32.84488081145695 37.0 34.0 38.0 11.0 38.0 7 33.08769120070561 38.0 34.0 38.0 12.0 38.0 8 32.98495926467516 38.0 34.0 38.0 11.0 38.0 9 33.34059837987141 38.0 34.0 38.0 12.0 38.0 10-11 33.0971728988232 38.0 34.0 38.0 11.0 38.0 12-13 33.154991527980876 38.0 34.0 38.0 11.0 38.0 14-15 33.07311468560685 38.0 34.0 38.0 11.0 38.0 16-17 33.10637606480515 38.0 34.0 38.0 11.0 38.0 18-19 33.09530441241325 38.0 34.0 38.0 11.0 38.0 20-21 33.19175776988604 38.0 34.0 38.0 11.0 38.0 22-23 33.118480607200055 38.0 34.0 38.0 11.0 38.0 24-25 33.12868184666806 38.0 34.0 38.0 11.0 38.0 26-27 33.11750574472529 38.0 34.0 38.0 11.0 38.0 28-29 33.17135529095003 38.0 34.0 38.0 11.0 38.0 30-31 33.183947264582315 38.0 34.0 38.0 11.0 38.0 32-33 33.268574611795835 38.0 34.0 38.0 11.0 38.0 34-35 33.25980084952302 38.0 34.0 38.0 11.0 38.0 36-37 33.168314648469234 38.0 34.0 38.0 11.0 38.0 38-39 33.17822575029594 38.0 34.0 38.0 11.0 38.0 40-41 33.18132442030499 38.0 34.0 38.0 11.0 38.0 42-43 33.29476823805213 38.0 34.0 38.0 11.0 38.0 44-45 33.33130236984425 38.0 34.0 38.0 11.0 38.0 46-47 33.24632685746118 38.0 34.0 38.0 11.0 38.0 48-49 33.1872316226818 38.0 34.0 38.0 11.0 38.0 50-51 33.1715293735348 38.0 34.0 38.0 11.0 38.0 52-53 33.227792864935125 38.0 34.0 38.0 11.0 38.0 54-55 33.186546897848345 38.0 34.0 38.0 11.0 38.0 56-57 33.12014019450828 38.0 34.0 38.0 11.0 38.0 58-59 33.16479818025671 38.0 34.0 38.0 11.0 38.0 60-61 33.224032681103914 38.0 34.0 38.0 11.0 38.0 62-63 33.03561729684562 38.0 34.0 38.0 11.0 38.0 64-65 33.01631734094654 38.0 34.0 38.0 11.0 38.0 66-67 33.04158252675069 38.0 34.0 38.0 11.0 38.0 68-69 32.97435183250934 38.0 34.0 38.0 11.0 38.0 70-71 32.87163150198454 38.0 33.5 38.0 11.0 38.0 72-73 32.934092333402965 38.0 34.0 38.0 11.0 38.0 74-75 33.012034909361006 38.0 34.0 38.0 11.0 38.0 76 31.671146391848293 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.203172948023564 1101 2 0.49596558018727066 1101 3 0.7078446560744389 1101 4 0.3719894006471769 1101 5 -0.2270611324647085 1101 6 -0.036867359400787336 1101 7 -0.3062226322811128 1101 8 -0.17133757606333688 1101 9 -0.023324770551496954 1101 10-11 -0.43789053312141135 1101 12-13 -0.04988617825352293 1101 14-15 0.08031643593510296 1101 16-17 -0.038595845187579414 1101 18-19 0.12609087734424662 1101 20-21 -0.1544271728374511 1101 22-23 0.017559849498397284 1101 24-25 0.03397194833724626 1101 26-27 -0.09218766632833564 1101 28-29 -0.40353955047301326 1101 30-31 -0.3094915496502182 1101 32-33 0.0338134710338025 1101 34-35 0.026458167288275547 1101 36-37 -0.5654918620108091 1101 38-39 0.05553706702176697 1101 40-41 -0.1963158724975358 1101 42-43 -0.2771465572677698 1101 44-45 0.2862892346296704 1101 46-47 0.8012873806693364 1101 48-49 0.01425693528564409 1101 50-51 0.37823333600888986 1101 52-53 0.24197357437595457 1101 54-55 0.9210220109082385 1101 56-57 0.5582669135336786 1101 58-59 0.3614054371113511 1101 60-61 0.48082480040380915 1101 62-63 0.3352449812256566 1101 64-65 -0.25643599038806997 1101 66-67 -0.052865851138371056 1101 68-69 0.32947816951688225 1101 70-71 0.9152547105658257 1101 72-73 0.031125246867404144 1101 74-75 0.520552931481248 1101 76 -1.560099622621319 1102 1 0.9587504220736314 1102 2 0.8496361452147454 1102 3 0.5426105195848407 1102 4 0.5825948207777465 1102 5 0.04991035783635667 1102 6 0.31450176370287153 1102 7 -0.02624517701832474 1102 8 0.5447866078641397 1102 9 -0.011300910704612477 1102 10-11 0.7225998149129751 1102 12-13 0.7962459902683818 1102 14-15 0.9201313812171392 1102 16-17 0.20376007985117184 1102 18-19 0.4453807181220384 1102 20-21 0.5812592674424835 1102 22-23 0.7362203163623349 1102 24-25 0.49200585937947494 1102 26-27 0.35894191894242766 1102 28-29 0.3783636010933762 1102 30-31 0.41973676278227146 1102 32-33 0.3902081918078366 1102 34-35 0.2239124907113137 1102 36-37 0.19405442515277826 1102 38-39 -0.12052324015028404 1102 40-41 0.48681314915480556 1102 42-43 0.4260613709648169 1102 44-45 0.28995275442676416 1102 46-47 -0.08129211301343275 1102 48-49 0.35076062228954186 1102 50-51 0.0604464777119631 1102 52-53 0.7730430403474884 1102 54-55 1.634321660546334E-4 1102 56-57 0.4500756590129882 1102 58-59 0.35438369083358623 1102 60-61 0.028497525968603554 1102 62-63 0.26523887535933 1102 64-65 0.314204186212379 1102 66-67 0.2529910837167293 1102 68-69 -0.24766710313353002 1102 70-71 0.2329006642551832 1102 72-73 0.06905207040787076 1102 74-75 0.20300091419694866 1102 76 0.7906119456469618 1103 1 -0.957737063950276 1103 2 -1.3064660307549794 1103 3 -0.5651128828405483 1103 4 -0.37384278135521853 1103 5 -0.9980383821653618 1103 6 -0.6688559381978791 1103 7 -1.1396020188519884 1103 8 -1.4799403208362953 1103 9 -1.0066995517986186 1103 10-11 -1.4644862778022585 1103 12-13 -1.0590047192869605 1103 14-15 -1.5352311370592808 1103 16-17 -1.2199304680115617 1103 18-19 -0.5636714879500317 1103 20-21 -1.093613872662445 1103 22-23 -0.662485282674389 1103 24-25 -1.8536120988212659 1103 26-27 -1.2379231365318972 1103 28-29 -1.444204559223131 1103 30-31 -1.4533388371145257 1103 32-33 -1.2774833881353942 1103 34-35 -1.7231503801926138 1103 36-37 -1.064524744022421 1103 38-39 -1.3406359089384452 1103 40-41 -1.7693793338588648 1103 42-43 -1.829486061734933 1103 44-45 -1.4681318761744215 1103 46-47 -1.2990395973172113 1103 48-49 -1.263628300046399 1103 50-51 -1.7261923801189525 1103 52-53 -0.8577086582850058 1103 54-55 -1.4456308397871638 1103 56-57 -0.6799664842741819 1103 58-59 -1.7231943602544 1103 60-61 -1.3967196409704208 1103 62-63 -1.4478212433299262 1103 64-65 -0.8982108821629566 1103 66-67 -1.7188417192419045 1103 68-69 -1.6211297332186803 1103 70-71 -1.6175121679457334 1103 72-73 -1.2403355618274432 1103 74-75 -1.547859152888286 1103 76 -1.6007185781338933 1104 1 -0.08107124610798877 1104 2 0.05207738661196615 1104 3 -0.9871908049184697 1104 4 -0.887084441655702 1104 5 -0.22845175963756148 1104 6 -0.2952458881172433 1104 7 -0.11320358068884673 1104 8 0.20357550973835714 1104 9 0.15954270267892667 1104 10-11 -0.13278693139702824 1104 12-13 -0.43015317867071445 1104 14-15 -0.3244256027243324 1104 16-17 -0.013008305207044657 1104 18-19 0.010112571128139791 1104 20-21 -0.4805292448719385 1104 22-23 -0.21432723157516165 1104 24-25 0.14897681553235742 1104 26-27 0.30156841766554265 1104 28-29 0.26430914929058247 1104 30-31 0.6345357066423745 1104 32-33 0.9944587220420118 1104 34-35 0.5426589738520349 1104 36-37 0.31248060357116714 1104 38-39 0.32545780127291124 1104 40-41 -0.2428323514295343 1104 42-43 0.09227355120115277 1104 44-45 0.16252341624557687 1104 46-47 0.7148599018764088 1104 48-49 0.6095148966262016 1104 50-51 0.08488053389513794 1104 52-53 0.07419440597099936 1104 54-55 0.9559267529469011 1104 56-57 0.8105266826895772 1104 58-59 0.7315208591667002 1104 60-61 0.3444738061053769 1104 62-63 0.38370833819965355 1104 64-65 0.7795117220302359 1104 66-67 0.9407372638664881 1104 68-69 0.4734080819073725 1104 70-71 0.41497658513125657 1104 72-73 0.2851928313480059 1104 74-75 0.03466779728572078 1104 76 0.8330306791447732 1105 1 -0.5774031121877528 1105 2 -0.6874184117073625 1105 3 -0.5314084919933002 1105 4 0.0773013126460178 1105 5 -0.22778482619752083 1105 6 -0.11690788624982673 1105 7 -0.294209316316433 1105 8 -1.261944031411435 1105 9 -0.11523387461866008 1105 10-11 -0.738295801611784 1105 12-13 -1.7052632226883233 1105 14-15 -0.495960883503308 1105 16-17 -0.12732069683035974 1105 18-19 -0.6927685813328424 1105 20-21 -0.22889525794382592 1105 22-23 -0.9279460124208327 1105 24-25 -0.7667227976462456 1105 26-27 -0.9093826294199587 1105 28-29 -0.74816250603822 1105 30-31 -0.4015453924639374 1105 32-33 -0.4288439323530824 1105 34-35 -0.7593284877992943 1105 36-37 -1.2055265993099908 1105 38-39 -0.7495722107318912 1105 40-41 -0.4569179856832335 1105 42-43 -0.3519041198672781 1105 44-45 -0.5434256300396072 1105 46-47 -0.5140983480902435 1105 48-49 -0.3239251460575332 1105 50-51 -1.0856543467237714 1105 52-53 -0.33343531258308445 1105 54-55 -0.5574119158539546 1105 56-57 -0.7084142270076441 1105 58-59 -0.5701331357646069 1105 60-61 -0.6088408530916354 1105 62-63 -0.8419152445667919 1105 64-65 -1.0115380312043953 1105 66-67 -0.916739059996388 1105 68-69 -0.6738508216540566 1105 70-71 -0.7991757424664456 1105 72-73 -0.22100964964438674 1105 74-75 -0.5350267471987422 1105 76 -0.4677563023144735 1106 1 -0.20841256294792387 1106 2 -0.8750182838709897 1106 3 -0.37645254988491317 1106 4 -0.05592244665636059 1106 5 -0.9125768638179395 1106 6 -0.2942808488576887 1106 7 -1.265811379960681 1106 8 -1.0914194457382393 1106 9 -0.9786772385414082 1106 10-11 -1.080258089882797 1106 12-13 -0.3706058698142911 1106 14-15 -1.3958604406416057 1106 16-17 -0.46626969925011963 1106 18-19 -1.1445788356359046 1106 20-21 -0.44254179038231456 1106 22-23 -0.737378510252185 1106 24-25 -0.6893133103614062 1106 26-27 -0.8606678414913631 1106 28-29 -1.111902632961474 1106 30-31 -1.0290165151412012 1106 32-33 -0.1799282587010751 1106 34-35 -0.747686247010364 1106 36-37 -1.2295644023506682 1106 38-39 -0.7093494102022859 1106 40-41 -0.7807320750773812 1106 42-43 -0.7311900624322263 1106 44-45 -0.6630896030113433 1106 46-47 -0.10351532293387322 1106 48-49 -1.109570948002471 1106 50-51 -0.5116233920600308 1106 52-53 -0.8918976236820271 1106 54-55 -0.8034056153471667 1106 56-57 -0.4368304257018778 1106 58-59 -0.5496564887299513 1106 60-61 -0.3563640234335992 1106 62-63 -0.6953371502418122 1106 64-65 -0.2676216727146894 1106 66-67 -0.3290570073095367 1106 68-69 -0.33373759515540513 1106 70-71 -0.37688199275180523 1106 72-73 -0.6294011941816677 1106 74-75 -0.8590462879009237 1106 76 -0.7160327941192506 1107 1 0.11677236822338699 1107 2 -0.35338990979564855 1107 3 -0.08208131586737366 1107 4 -0.9354599962571051 1107 5 -0.06595951213028783 1107 6 -0.18155856403919302 1107 7 0.14990409547164063 1107 8 -1.2841397710211488 1107 9 -0.39796886642560025 1107 10-11 -0.5903986865613788 1107 12-13 -0.2878889737186583 1107 14-15 0.16772648925601175 1107 16-17 -0.09070020921925703 1107 18-19 -0.45847194296946725 1107 20-21 -0.32256922388057063 1107 22-23 0.05400443199050642 1107 24-25 -0.20957000952370208 1107 26-27 -0.2947294688856701 1107 28-29 -0.08767787422929274 1107 30-31 -0.9455892873941778 1107 32-33 -0.48316639126365857 1107 34-35 -0.4722735198267003 1107 36-37 -0.16240132000118734 1107 38-39 0.3638147498917732 1107 40-41 0.27477177051121515 1107 42-43 -0.16892581905789683 1107 44-45 -0.21386606898890648 1107 46-47 0.3371107486871523 1107 48-49 -0.259646880006585 1107 50-51 0.07746199470330595 1107 52-53 -0.008220555147268271 1107 54-55 -0.07007391496016169 1107 56-57 0.11399502866543543 1107 58-59 -0.9004623477413674 1107 60-61 0.2116318615416617 1107 62-63 -0.3944749545821793 1107 64-65 -0.3014481558381874 1107 66-67 -1.035885988840917 1107 68-69 -0.3737366153876067 1107 70-71 -0.4021600018636349 1107 72-73 -0.5111432213212339 1107 74-75 -0.8277672010445087 1107 76 0.26192243049251474 1108 1 -1.0724556786688915 1108 2 -1.5499725242614737 1108 3 -0.7750695927972586 1108 4 -1.3705960281084657 1108 5 -0.6311552652822456 1108 6 -1.2781632975052375 1108 7 -1.375532754782725 1108 8 -1.8608927017886785 1108 9 -2.1690372141362815 1108 10-11 -1.6917590050749851 1108 12-13 -1.3831389184211602 1108 14-15 -1.564993041821186 1108 16-17 -1.280536528617624 1108 18-19 -1.4927840420625884 1108 20-21 -1.588743742792314 1108 22-23 -2.1473337675228734 1108 24-25 -1.5224432676524344 1108 26-27 -1.6134642620730233 1108 28-29 -1.789983780002352 1108 30-31 -2.000416759192447 1108 32-33 -1.7103838210358333 1108 34-35 -2.2215270035692356 1108 36-37 -2.325888380386054 1108 38-39 -1.7340341773367136 1108 40-41 -2.0356131000926325 1108 42-43 -1.5556765379254074 1108 44-45 -1.4924825255250695 1108 46-47 -1.8499053981830116 1108 48-49 -1.6840828455009458 1108 50-51 -1.8014088303354043 1108 52-53 -2.5941805197568684 1108 54-55 -1.6225788917352126 1108 56-57 -2.6117846660923654 1108 58-59 -1.5976532347132775 1108 60-61 -1.772801892052673 1108 62-63 -1.6263926719013568 1108 64-65 -1.5443147782668518 1108 66-67 -1.520789771189957 1108 68-69 -1.1839002959892468 1108 70-71 -0.8875338129673764 1108 72-73 -1.2333009297928115 1108 74-75 -1.4320582870874219 1108 76 -1.0628397902551043 1109 1 -1.434381387368795 1109 2 -1.171673404026869 1109 3 -1.8259004823378255 1109 4 -1.3075461747360322 1109 5 -1.9222109837573171 1109 6 -1.798935536019414 1109 7 -1.9043695094259334 1109 8 -1.799797882629342 1109 9 -1.4417644868635549 1109 10-11 -1.5209378907054827 1109 12-13 -2.0999033367984836 1109 14-15 -1.5747682128866778 1109 16-17 -2.0362794416185963 1109 18-19 -1.276019623685265 1109 20-21 -1.4762070859008176 1109 22-23 -1.589538068598145 1109 24-25 -1.5763288981509689 1109 26-27 -1.4051309287396876 1109 28-29 -1.9314793811753717 1109 30-31 -1.4344343545003646 1109 32-33 -1.9531746323750312 1109 34-35 -1.7064976780560421 1109 36-37 -1.8390848613244728 1109 38-39 -1.507005829340624 1109 40-41 -2.2463902261923394 1109 42-43 -1.6812874861170002 1109 44-45 -1.5632303261115794 1109 46-47 -1.6151791029728457 1109 48-49 -1.0803438425683964 1109 50-51 -0.9735740307264109 1109 52-53 -1.8219420055828692 1109 54-55 -2.0291038184214294 1109 56-57 -1.8383242555351806 1109 58-59 -1.6897842220055566 1109 60-61 -2.2025428500194373 1109 62-63 -2.0387827012268716 1109 64-65 -2.029285452753655 1109 66-67 -1.1303938767618078 1109 68-69 -1.8354154475043956 1109 70-71 -2.1931300984023707 1109 72-73 -1.9889216531554723 1109 74-75 -1.3519018843503723 1109 76 -1.3837097804799328 1110 1 -0.9292738604870721 1110 2 -0.4636088878978377 1110 3 0.3211425284148639 1110 4 -0.2645354220478602 1110 5 -1.369791628918609 1110 6 -0.831193600535542 1110 7 -0.5785630578130281 1110 8 -0.007862398758376798 1110 9 -0.2000978201968877 1110 10-11 -0.36448627780225706 1110 12-13 0.18087623309398992 1110 14-15 -0.3843112236393651 1110 16-17 0.2084786228975375 1110 18-19 -0.2984658602444057 1110 20-21 -0.5497285912771659 1110 22-23 -0.547333767522872 1110 24-25 -0.21638266159182962 1110 26-27 0.1573690712603124 1110 28-29 0.5865313715127982 1110 30-31 0.2101893014136138 1110 32-33 -0.15129291194492112 1110 34-35 0.12468511764288337 1110 36-37 0.4578237408260648 1110 38-39 0.3511930953905633 1110 40-41 -0.0019009788805135486 1110 42-43 -0.029540174289046206 1110 44-45 0.0749417168991684 1110 46-47 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-1.002973571276108 2116 40-41 -0.1863308034419191 2116 42-43 -0.33896999885045176 2116 44-45 -0.20181266906573825 2116 46-47 0.12696860500678042 2116 48-49 -0.22953739095549253 2116 50-51 0.3309675651988897 2116 52-53 0.3637540894935327 2116 54-55 0.9581627350590409 2116 56-57 0.7285263386923262 2116 58-59 1.1527454893696572 2116 60-61 0.22699874590585978 2116 62-63 0.6176264668546168 2116 64-65 -0.1950324816161384 2116 66-67 0.18794228622630982 2116 68-69 0.5395845524956044 2116 70-71 0.5893401902224156 2116 72-73 0.4656623875915571 2116 74-75 0.5360437862778085 2116 76 0.0356450582297434 2117 1 -0.8849044911177053 2117 2 -1.2041494284383774 2117 3 -0.7169205346481995 2117 4 -0.4559768634893011 2117 5 -0.17722406135104052 2117 6 -1.3987611681031105 2117 7 -0.5028873821373523 2117 8 0.6448403039443278 2117 9 -0.3201428652419338 2117 10-11 -0.18070249401847605 2117 12-13 -0.9692363795186196 2117 14-15 0.11017075834261192 2117 16-17 -0.8790213771024682 2117 18-19 -0.19722712150566934 2117 20-21 -0.22664300569157803 2117 22-23 -0.7914779116670161 2117 24-25 -0.6551214003305645 2117 26-27 -0.9625633611721227 2117 28-29 -0.6508560158745809 2117 30-31 -0.5197656535413486 2117 32-33 -1.0130046236566344 2117 34-35 -0.9933329003751368 2117 36-37 -0.9760726555703343 2117 38-39 -0.631351949654487 2117 40-41 0.6554188409393049 2117 42-43 -0.980328462577333 2117 44-45 -0.4110943191368648 2117 46-47 -0.46620351448112984 2117 48-49 -1.3072400936581943 2117 50-51 -1.0162941702207462 2117 52-53 -0.5537423543187003 2117 54-55 -0.39745604161236514 2117 56-57 -1.2794750837827813 2117 58-59 -0.6054337424937799 2117 60-61 -1.0898575841083655 2117 62-63 -0.9961715416802264 2117 64-65 -0.4613909453430267 2117 66-67 0.02097927057908322 2117 68-69 -1.5786586907476412 2117 70-71 0.24954644911288426 2117 72-73 -1.3353689068607935 2117 74-75 -0.4447527997819378 2117 76 -0.7075981850134987 2118 1 0.7188444190828172 2118 2 1.5138104669408712 2118 3 1.4321640337912065 2118 4 1.0230989865542917 2118 5 1.610047080758811 2118 6 1.5720322059160736 2118 7 1.5795014583160025 2118 8 1.700202117370658 2118 9 0.8969451905557975 2118 10-11 0.930675012520318 2118 12-13 1.0654192438466765 2118 14-15 1.4776511419520304 2118 16-17 1.2096883003168912 2118 18-19 0.9538190859921514 2118 20-21 1.3221800108733746 2118 22-23 1.309655479788951 2118 24-25 2.0970581986232233 2118 26-27 1.6714819744861202 2118 28-29 1.4757786833407636 2118 30-31 1.456694677757703 2118 32-33 1.2685995611733603 2118 34-35 1.5193087735568582 2118 36-37 1.5520441709335913 2118 38-39 1.247026428723892 2118 40-41 1.4189323544528207 2118 42-43 2.0203254171088005 2118 44-45 1.2351567706626128 2118 46-47 1.8243370260594105 2118 48-49 1.3712690606574114 2118 50-51 1.2844904854026282 2118 52-53 1.8111225105461628 2118 54-55 1.2934768267398553 2118 56-57 1.4842563896261112 2118 58-59 1.1005383586396036 2118 60-61 1.1361668273999186 2118 62-63 1.5620237503860324 2118 64-65 1.2328113214398968 2118 66-67 1.3575093490446477 2118 68-69 1.2452297137859247 2118 70-71 1.3149344177266613 2118 72-73 1.942529959747759 2118 74-75 1.1480981156496313 2118 76 1.3259676388749035 2119 1 0.9299366658287163 2119 2 1.5556893577161972 2119 3 0.6794758617481982 2119 4 1.4503768586538968 2119 5 1.6236294237129698 2119 6 0.642490609990773 2119 7 0.6635422053448679 2119 8 0.25792707492583844 2119 9 0.4529723552417053 2119 10-11 2.1276189853556318 2119 12-13 1.9021481629185573 2119 14-15 1.4659080746062472 2119 16-17 1.8753645878098126 2119 18-19 1.5333323853696257 2119 20-21 0.823736321003544 2119 22-23 0.9009118465122157 2119 24-25 1.145020847180099 2119 26-27 1.5619743344182027 2119 28-29 1.2760050557233313 2119 30-31 1.8386980733434442 2119 32-33 1.7732684915638544 2119 34-35 2.169421959748149 2119 36-37 1.1483939162646593 2119 38-39 2.0014123936361727 2119 40-41 1.5461253369089576 2119 42-43 1.395679123956569 2119 44-45 1.1293276818114535 2119 46-47 1.512933517287479 2119 48-49 1.084059100272576 2119 50-51 0.6533359862515198 2119 52-53 1.7277891772128342 2119 54-55 2.528338173655534 2119 56-57 1.4675614264116277 2119 58-59 1.3895875946328218 2119 60-61 2.0077005002918185 2119 62-63 1.3456966422932197 2119 64-65 1.2172482201382522 2119 66-67 0.4796089528929812 2119 68-69 0.8970406928464811 2119 70-71 1.5608314182925938 2119 72-73 0.9678553700477011 2119 74-75 0.9570964178567607 2119 76 2.0531889178788667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 7.0 13 104.0 14 490.0 15 955.0 16 1144.0 17 1234.0 18 1234.0 19 946.0 20 695.0 21 482.0 22 330.0 23 258.0 24 220.0 25 210.0 26 224.0 27 270.0 28 289.0 29 367.0 30 460.0 31 567.0 32 778.0 33 1025.0 34 1589.0 35 2637.0 36 5901.0 37 20667.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.03470046189913 20.367662419051598 12.271661676299237 32.325975442750035 2 18.294919109625607 19.04695587586751 31.60875519346378 31.049369821043104 3 20.14947891279623 21.18933221920479 27.655919968433025 31.00526889956595 4 16.619084093493957 18.617552166747906 30.27876424575819 34.48459949399995 5 14.880579346842143 31.467168024510826 30.046654132720562 23.605598495926465 6 28.30582828493837 34.30587470696098 16.470533621149876 20.917763386950767 7 26.425736369333613 29.471021052387254 19.246570573079868 24.85667200519927 8 23.6218462038391 30.877608337395262 21.04774505025184 24.4528004085138 9 23.686837035489635 17.540561242253325 20.548708307220945 38.223893415036095 10-11 25.290717916579624 23.389736090801478 24.799805027505048 26.519740965113854 12-13 23.805213193138826 20.59513032982847 24.769630712810155 30.830025764222547 14-15 23.81565814822552 21.806745119884873 22.71429566186199 31.66330107002762 16-17 25.27679130979737 23.38857554023629 23.296892045586425 28.037741104379915 18-19 23.981616879047422 22.87793329155351 23.985098530742984 29.155351298656086 20-21 23.316621405194624 23.15066267437272 24.355314161037995 29.177401759394655 22-23 25.897685862173013 22.754914931643572 23.019520460506467 28.327878745676948 24-25 24.436552700601165 24.417983891558155 22.67019474038484 28.47526866745584 26-27 23.551052619362626 24.690713274377366 24.27175452034445 27.486479585915557 28-29 24.676786667595106 23.563818675579697 25.588979411832973 26.170415244992224 30-31 24.44003435229673 23.1332544158949 24.764988510549404 27.661722721258965 32-33 23.441960866234943 24.391291228558828 25.13172248914885 27.03502541605738 34-35 23.48606178771209 24.066337070306155 25.209479377016457 27.2381217649653 36-37 24.054731564654272 22.882575493814265 25.332497736926396 27.730195204605064 38-39 23.18315809019799 22.42880022282571 27.571199777174293 26.816841909802008 40-41 22.730543369774622 22.456653436390223 27.203305248009656 27.609497945825503 42-43 21.68488731054012 22.57851124573498 26.87719053919179 28.85941090453311 44-45 21.061671657034097 21.906552468491054 28.683007218624518 28.348768655850336 46-47 21.026855140078453 23.243506719587774 28.513566836107053 27.216071304226723 48-49 21.16496065733584 23.554534271058188 27.447020866699162 27.833484204906807 50-51 21.470185455980317 24.65705730798691 26.163451941601096 27.70930529443168 52-53 20.62530464452336 24.339066453125362 26.399043706334286 28.636585196016988 54-55 20.636910150175243 23.57542418123158 26.58589234732957 29.20177332126361 56-57 20.951419353341226 23.656662720794746 26.45010793120256 28.941809994661465 58-59 20.8852679711255 23.859759069702665 25.594782164658913 29.66019079451292 60-61 20.64387345356637 22.948726876029987 26.55339693150431 29.854002738899332 62-63 20.95490100503679 23.559176473318942 26.121672121254324 29.364250400389945 64-65 22.023768075575052 23.59863519253534 24.40289673421071 29.9746999976789 66-67 21.225309286725622 24.825337139939187 23.289928742195297 30.65942483113989 68-69 21.062832207599286 25.456676647401526 23.60327739479609 29.877213750203097 70-71 22.732864470905 24.493419678295382 23.88993338439756 28.883782466402064 72-73 22.023768075575052 24.723208690202632 23.583548035187892 29.669475199034423 74-75 21.455098298632873 25.681823457048026 23.681034282663695 29.182043961655406 76 21.024534038948076 24.71276373511594 23.354919573845834 30.907782652090148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 3.0 17 5.0 18 8.0 19 10.0 20 8.0 21 7.0 22 16.0 23 41.0 24 51.5 25 46.0 26 69.5 27 111.0 28 155.0 29 181.0 30 217.0 31 285.5 32 411.0 33 504.0 34 558.0 35 715.5 36 860.0 37 901.0 38 1076.0 39 1401.5 40 1705.5 41 1902.5 42 1946.0 43 2014.0 44 2178.0 45 2307.5 46 2341.0 47 2299.5 48 2170.0 49 1998.5 50 1915.0 51 1811.0 52 1597.0 53 1346.0 54 1205.0 55 1148.0 56 1049.5 57 977.5 58 947.0 59 915.5 60 907.5 61 955.0 62 979.0 63 1027.0 64 1112.5 65 1178.0 66 1169.5 67 1133.0 68 1106.0 69 1040.5 70 944.5 71 887.0 72 822.0 73 593.5 74 375.0 75 320.0 76 225.0 77 98.0 78 47.5 79 29.0 80 17.5 81 5.5 82 5.0 83 5.0 84 3.0 85 1.5 86 2.5 87 3.0 88 3.5 89 3.5 90 2.0 91 2.0 92 3.0 93 3.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.5 99 4.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 43083.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.40289673421071 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62533146155046 60.73161107629459 2 11.109031352363125 16.530882250539655 3 3.6063016690063954 8.049578720144837 4 1.6970831383559506 5.050716059698721 5 0.8079862735922634 3.0058259638372444 6 0.4523475276867884 2.019357983427338 7 0.22773358290438311 1.186082677622264 8 0.1965372016846046 1.1698349697096302 9 0.10918733426922478 0.7311468560685189 >10 0.16846045858680395 1.5249634426571965 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011605505651881252 0.0 2 0.0 0.0 0.0 0.011605505651881252 0.0 3 0.0 0.0 0.0 0.011605505651881252 0.0 4 0.0 0.0 0.0 0.013926606782257503 0.0 5 0.0 0.0 0.0 0.018568809043010004 0.0 6 0.0 0.0 0.0 0.018568809043010004 0.0 7 0.0 0.0 0.0 0.018568809043010004 0.0 8 0.0 0.0 0.0 0.018568809043010004 0.0 9 0.0 0.0 0.0 0.018568809043010004 0.0 10 0.0 0.0 0.0 0.023211011303762505 0.0 11 0.0 0.0 0.0 0.025532112434138755 0.0 12 0.0 0.0 0.0 0.025532112434138755 0.0 13 0.0 0.0 0.0 0.025532112434138755 0.0 14 0.0 0.0 0.0 0.025532112434138755 0.0 15 0.0 0.0 0.0 0.025532112434138755 0.0 16 0.0 0.0 0.0 0.025532112434138755 0.0 17 0.0 0.0 0.0 0.025532112434138755 0.0 18 0.0 0.0 0.0 0.025532112434138755 0.0 19 0.0 0.0 0.0 0.03249541582526751 0.0 20 0.0 0.0 0.0 0.03249541582526751 0.0 21 0.0 0.0 0.0 0.03945871921639626 0.0 22 0.0 0.0 0.0 0.04642202260752501 0.0 23 0.0 0.0 0.0 0.04874312373790126 0.0 24 0.0 0.0 0.0 0.05106422486827751 0.0 25 0.0 0.0 0.0 0.05338532599865376 0.0 26 0.0 0.0 0.0 0.06499083165053501 0.0 27 0.0 0.0 0.0 0.10677065199730752 0.0 28 0.0 0.0 0.0 0.1601559779959613 0.0 29 0.0 0.0 0.0 0.21354130399461504 0.0 30 0.0 0.0 0.0 0.29245874242740755 0.0 31 0.0 0.0 0.0 0.36673397859944756 0.0 32 0.0 0.0 0.0 0.4456514170322401 0.0 33 0.0 0.0 0.0 0.5825963837244389 0.0 34 0.0 0.0 0.0 0.8031009911101826 0.0 35 0.0 0.0 0.0 1.0143211939744214 0.0 36 0.0 0.0 0.0 1.3856973748346215 0.0 37 0.0 0.0 0.0 1.9056240280389016 0.0 38 0.0 0.0 0.0 2.402339669939419 0.0 39 0.0 0.0 0.0 2.8990553118399367 0.0 40 0.0 0.0 0.0 3.3331012232202957 0.0 41 0.0 0.0 0.0 3.9017710001624772 0.0 42 0.0 0.0 0.0 4.551679316667827 0.0 43 0.0 0.0 0.0 5.196945430912425 0.0 44 0.0 0.0 0.0 5.754009702202725 0.0 45 0.0 0.0 0.0 6.325000580275282 0.0 46 0.0 0.0 0.0 6.909918065130098 0.0 47 0.0 0.0 0.0 7.473945639811527 0.0 48 0.0 0.0 0.0 7.935844764756401 0.0 49 0.0 0.0 0.0 8.390780586310145 0.0 50 0.0 0.0 0.0 8.827147598820881 0.0 51 0.0 0.0 0.0 9.356358656546666 0.0 52 0.0 0.0 0.0 10.068936703572176 0.0 53 0.0 0.0 0.0 10.867395492421604 0.0 54 0.0 0.0 0.0 11.505698303275073 0.0 55 0.0 0.0 0.0 12.055799271174244 0.0 56 0.0 0.0 0.0 12.559478216465891 0.0 57 0.0 0.0 0.0 13.014414038019636 0.0 58 0.0 0.0 0.0 13.385790218879837 0.0 59 0.0 0.0 0.0 13.726992085045145 0.0 60 0.0 0.0 0.0 14.202817816772276 0.0 61 0.0 0.0 0.0 14.62757932363113 0.0 62 0.0 0.0 0.0 15.121973864401271 0.0 63 0.0 0.0 0.0 15.641900517605553 0.0 64 0.0 0.0 0.0 16.215212496808487 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGATAA 15 0.0022103465 70.0 43 TAAGAGC 15 0.0022103465 70.0 32 CTCGTAC 20 0.0069068973 52.5 45 TAACGTC 20 0.0069068973 52.5 16 AGTGAGC 20 0.0069068973 52.5 8 GTCCGCG 20 0.0069068973 52.5 23 CACGTAC 20 0.0069068973 52.5 35 CAAAGAG 20 0.0069068973 52.5 36 CAATCTC 50 0.007134738 28.000002 5 TGACGCT 495 5.6216225E-4 7.777777 70 ATCTGAC 565 2.7874042E-4 7.4336286 67 CTGACGC 530 0.0011658954 7.264151 69 TCTGACG 535 0.0012882171 7.196262 68 CATCTGA 580 0.0030230882 6.637931 66 ACATCTG 645 0.009146477 5.968992 65 >>END_MODULE