FastQCFastQC Report
Wed 25 May 2016
SRR1780196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43083
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT26216.083606062716153No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG15123.509504909128891RNA PCR Primer, Index 18 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG6361.4762203189192953No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4170.9678991713668964No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC2730.6336606085927163No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT1930.44797251816261635RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1280.29710094468816006No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT1200.27853213564515006No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC770.1787247870389713No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG650.1508715734744563No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG630.14622937121370377No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT600.13926606782257503No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGTCTTCTG460.10677065199730754RNA PCR Primer, Index 30 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC450.10444955086693128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA150.002210346570.01
TCTTTAT352.9087278E-760.0000041
TTATCCC200.006906897352.553
AAGTTAA200.006906897352.519
GCTTATA200.006906897352.53
TCAGAGC200.006906897352.58
GTCAACC200.006906897352.520
GCTCGTA200.006906897352.538
CGATCGG200.006906897352.526
CTTTATA451.6480135E-646.6666642
TTTATAC503.4032018E-642.0000043
CGGAACT556.550319E-638.18181634
GCCGGAA708.444258E-735.00000432
CCGGAAC601.1896227E-535.033
AACTCGT601.1896227E-535.037
ACTCGTA652.0576521E-532.30769338
GGAACTC802.3965877E-630.62535
CTCGTAT7550.028.27814539
CTTGAAA7250.027.51724257
CGTATGC7900.027.46835541